MOLECULES
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2022
·Vol. 27
·期 9
·2906
·
IF 4.927
Shuying Han,Zhaohua Zhang,Jia Chen,Jie Li,Mi Zhou,Zejian He,Zhen He,Longfei Li
Tympanic membrane perforation (TMP), a common disease, often needs a scaffold as the patch to support surgery. Due to the environment of auditory meatus, the patch can be infected by bacteria that results in failure; therefore, the ideal scaffold may combine biomimetic and antibacterial features. In this work, gelatin was used as the electrospinning framework, genipin as the crosslinking agent, and levofloxacin as an antibacterial in order to prepare the scaffold for TMP. Different contents of levofloxacin have been added to gelatin/genipin. It was found that, with the addition of levofloxacin, the gelatin/genipin membranes exhibit improved hydrophilia and enhanced tensile strength. The antibacterial and cell-cultured experiments showed that the prepared antibacterial membranes had excellent antibacterial properties and good biocompatibility, respectively. In summary, levofloxacin is a good group for the gelatin/genipin scaffold because it improves the physical properties and antibacterial action. Compared with different amounts of levofloxacin, a gelatin/genipin membrane with 1% levofloxacin is more suitable for a TM.Keywords:gelatin;genipin;levofloxacin;nanofiber membrane;tympanic membrane
研究领域:
生物材料学
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耳科学
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组织工程学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
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2018
·
IF 1.911
Longfei Li,Weizheng Zhang,Mengjia Huang,Jie Li,Jia Chen,Mi Zhou,Jianguo He
The wound healing for tympanic membrane (TM) perforations is one of the most common clinical treatment in otomicrosurgery. Recently, tissue-engineered composites and grafts, also new designs of biomaterials are applied to the management of TM perforation. In this work, a series of gelatin/genipin nanofibrous films were prepared as a patch for repairing TM perforation. Gelatin, a type of biomaterial with excellent electrospinning performance, has been used for preparing the nanofibers. The genipin, as a crosslinking agent, has been blended into the gelatin nanofibers. The reaction between gelatin and genipin engender suitable tensile strength and water-resistance for TM patch. The survival rate of human umbilical vein endothelial cells and fibroblasts demonstrated that the gelatin/genipin nanofiber scaffolds had good biocompatibility, which indicated the genipin was a kind of effective and nontoxic crosslinking agent for improving the mechanical property and water-resistance of gelatin films. In short, our work provides a novel macromolecular material with good mechanical properties, water-tolerance and excellent biocompatibility which could be used as a potential patch for TM repair.
研究领域:
生物材料学
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组织工程学
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耳科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
International Journal of Polymeric Materials and Polymeric Biomaterials
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2023
·
IF 3.2
Yangjing Ren,Shuying Han,Jia Chen,Jie Li,Mi Zhou,Zejian He,Zhen He
This work focuses on developing a novel bioscaffold for tympanic membrane (TM) repair. A strategy to fabricate porous scaffolds is proposed, which is based on the chitosan membranes that were cross-linked and modified by tert-butyl acetoacetate esterified polyethylene glycol, while their mechanical properties and hydrophilicity were also improved. With two different crosslink methods, direct and freeze cross-linking, the chitosan-polyethylene glycol membranes were fabricated. Based on the morphology, hydrophilicity, and mechanical property, the biomedical scaffold has good enough properties for TM repair and the freeze cross-linking membrane is better. Our work provides a simple and reliable method for the preparation of bioscaffolds.
研究领域:
生物材料学
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耳科学
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组织工程学
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产品关联
DOI
高影响因子
MACROMOLECULAR RESEARCH
·
2018
·Vol. 26
·期 10
·892-899
·
IF 1.767
Huang Mengjia,Li Jie,Chen Jia,Zhou Mi,He Jianguo
The wound healing for tympanic membrane (TM) perforations is one of the most common clinic treatment. New designs of biomaterials, and lately tissue-engineered composites and grafts, have been applied for the management of TM perforation. In this work, a series of chitosan (CS)/polyvinyl alcohol (PVA) nanofibrous films have been fabricated as a kind of patch for repairing TM perforation. The scaffolds were crosslinked by glutaraldehyde solution to prevent them from being dissolved in the culture medium. The morphology of nanofibers from different precursors has been observed by using scanning electron microscopy (SEM). And the crosslinked films have been characterized by SEM and Fourier transform infrared spectroscopy for confirming its morphology and chemical structure. We found the films with crosslinking showed stronger tensile strength and modulus compared to the uncrosslinked one. And the mechanical properties could be tuned by the crosslinking time. In addition, after crosslinked, the films showed good thermostability and water-resistance, characterized by thermo gravimetric analyzer and contact angle meter. The cell viability studies with human umbilical vein endothelial cells, fibroblasts and mesenchymal stem cells demonstrated that the nanofibrous scaffolds formed by electrospinning process had no side effects for cell proliferation. However, a better way of crosslinking CS/PVA films needs to be further studied.
研究领域:
生物材料学
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组织工程学
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耳科学
✓ 已验证
产品关联
DOI
高影响因子
Heliyon
·
2024
·
IF 3.4
Yuan Lv,Linrong Wu,Jiangyu Yan,Zhisen Shen,Junjun Zhang,Xiaoqin Zhang,Tian Li
Perforation of the tympanic membrane (TM) is a common condition that often requires a scaffold as a support for surgery. However, because of the external environment of the auditory canal, the scaffold could become bacterially infected and prevent the TM from healing. As a result, the perfect scaffold should have both antibacterial and biomimetic qualities. In this study, the biodegradable biomaterial poly(1,4-butylene carbonate) (PBC) films containing levofloxacin (LEV) was successfully prepared for the first time. The results showed that the hydrophilicity of the LEV/PBC film was improved after the addition of LEV, and the tensile strength was also complied with the requirements of the standard. The created antibacterial film demonstrated excellent antibacterial properties. In vitro hemolysis experiments revealed no risk of hemolysis for the new material, and the cytotoxicity study further confirmed its non-cytotoxic nature. Overall, LEV was a good component of PBC/LEV film, which is expected to be used for TM repair in the future.
研究领域:
生物材料学
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耳科学
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抗菌材料研究
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产品关联
DOI
PubMed
高影响因子
International Journal of Polymeric Materials and Polymeric Biomaterials
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2022
·
IF 3.221
Jia Chen,Shuying Han,Mengjia Huang,Jie Li,Mi Zhou,Jianguo He
For treating tympanic membrane (TM) perforation, a scaffold is helpful for TM repair in myringoplasty. In this work, the green-crosslinked method respectively based on four kinds of polybasic organic acids (maleic acid, tartaric acid, citric acid, and malic acid) has been used to crosslink the composite nanofibrous membranes of chitosan (CS) and poly(vinyl alcohol) (PVA) for preparing the TM scaffold. The crosslinked membranes exhibit nanofibrous morphology, good physical properties, and excellent biocompatibility to promote TM cell proliferation. To summarize, the polybasic organic acids are effective and nontoxic crosslinked agents to prepare composite CS/PVA nanofiber membranes for TM scaffold.
研究领域:
生物材料学
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耳科学
✓ 已验证
产品关联
DOI
高影响因子
Materials Today Bio
·
2024
·
IF 8.7
Shengjia Chen,Xiangshu Guo,Yanyu Yang,Junjie Deng,Ting Xu,Zhechen Yuan,Hao Xue,Longxing Niu,Rong Wang,Yi Shen
The tympanic membrane (TM) is constantly in a state of vibrating. However, there is currently a lack of drug-delivery scaffolds suitable for the dynamic environment of TM perforation. In this study, a mechano-responsive tough hydrogel was developed. It consists of basic fibroblast growth factor (bFGF)-loaded sodium alginate (SA) microspheres, polysulfobetaine methacrylate (polySBMA), and gelatin methacrylate (GelMA). This hydrogel was designed to serve as a TM scaffold to promote perforation healing under dynamic conditions. bFGF was encapsulated in SA microspheres, which were then incorporated into polySBMA-GelMA hydrogels through photo-initiated free radical polymerization. The mechanical properties, tissue adhesiveness, swelling properties, and degradation of the hydrogels were evaluated before and after microsphere incorporation. It was observed that incorporating bFGF-loaded SA microspheres did not significantly impact the adhesion and degradation mechanisms of the hydrogel. The compressive strength and tensile strength of the microsphere-incorporated hydrogel were up to 6.6 MPa and 64.1 kPa, respectively, suitable for a TM scaffold. The release behavior of bFGF from the hydrogel could be controlled by vibration stimulation without significantly affecting the hydrogel's mechanical properties, indicating a mechano-responsive nature of the hydrogel. The in vitro cytotoxicity assay demonstrated that the hydrogels showed no cytotoxic effects. Moreover, cell culture assays demonstrated that vibration stimulation could enhance the release of bFGF, significantly promoting cell proliferation and migration. The results demonstrate the significant potential of the mechano-responsive hydrogel as a scaffold for repairing TM perforations.
研究领域:
生物材料学
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组织工程学
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耳科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MACROMOLECULAR BIOSCIENCE
·
2025
·
IF 4.1
Hao Xue,Shengjia Chen,Zhechen Yuan,Yi Hu,Juntao Huang,Yi Shen
Tympanic membrane perforation (TMP) often leads to hearing loss and requires effective repair strategies. However, existing surgical options are invasive and lack ideal biomaterials for scaffold-based healing. Herein, we present a custom-engineered mechano-acoustic responsive hydrogel incorporating bFGF-loaded sodium alginate microspheres, designed for controlled drug release and tissue regeneration under dual stimulation: vibrational simulation and low-frequency acoustic waves (generated by radio devices) — representing its first transition from in vitro characterization to in vivo regenerative application. This polymer-based scaffold exhibits robust adhesion, biocompatibility, and mechanoresponsive release behavior. A rat acute TMP model was employed to evaluate the hydrogel's therapeutic efficacy. Compared with control and blank hydrogel groups, the bFGF-loaded mechanoresponsive hydrogel (SGM) noticeably accelerated tympanic membrane closure, reduced local inflammation, and enhanced early auditory recovery, as confirmed by otoscopic inspection, ABR tests, histological staining, and TEM imaging. Our findings demonstrate that the SGM hydrogel effectively promotes functional tissue regeneration and early hearing restoration in vivo. This work highlights the potential of polymer-based, stimuli-responsive biomaterials in advancing minimally invasive strategies for TMP treatment and offers valuable insights for future tissue engineering applications in otolaryngology.
研究领域:
耳鼻喉科学
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组织工程
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生物材料学
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再生医学
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药剂学
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanoscale
·
2014
·Vol. 7
·期 3
·877-884
·
IF 6.739
Zenghui Wang,Hao Jia,Xuqian Zheng,Rui Yang,Zefang Wang,G. J. Ye,X. H. Chen,Jie Shan,Philip X.-L. Feng
We report on the experimental demonstration of a new type of nanoelectromechanical resonator based on black phosphorus crystals. Facilitated by a highly efficient dry transfer technique, crystalline black phosphorus flakes are harnessed to enable drumhead resonators vibrating at high and very high frequencies (HF and VHF bands, up to ∼100 MHz). We investigate the resonant vibrational responses from the black phosphorus crystals by devising both electrical and optical excitation schemes, in addition to measuring the undriven thermomechanical motions in these suspended nanostructures. Flakes with thicknesses from ∼200 nm down to ∼20 nm clearly exhibit elastic characteristics transitioning from the plate to the membrane regime. Both frequency- and time-domain measurements of the nanomechanical resonances show that very thin black phosphorus crystals hold interesting potential for moveable and vibratory devices and for semiconductor transducers where high-speed mechanical motions could be coupled to the attractive electronic and optoelectronic properties of black phosphorus.
研究领域:
材料科学
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纳米技术
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物理学
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电子学
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2022
·Vol. 10
·期 14
·2719-2727
·
IF 7.571
Zhili Zhang,Jin-Bo Li,Xu Li,Cheng-Ye Zhu,Liujie Ren,Xiao-Jun Huang,Jian Wu,Jian Ji,Zhi-Kang Xu
The tympanic membrane plays an important role in the human hearing system, which is easily perforated under unfavorable conditions, leading to loss of hearing and otitis media. Many autologous materials and artificial materials have been used to repair a perforated tympanic membrane, but these materials sometimes can cause severe hearing loss because of their adhesion to the ossicle during the healing process and the postoperative process. Herein, we report Janus membranes with asymmetric cellular adhesion behaviors for regenerating the eardrum. These Janus membranes are constructed by co-depositing a tannic acid (TA)/3-aminopropyltriethoxysilane (APTES) coating on one surface of the polypropylene microfiltration membrane. Cellular experiments indicate that the Janus membranes have good biocompatibility and asymmetric cellular adhesion properties. The repair of the tympanic membrane perforation experiment and laser Doppler vibrometer (LDV) measurements prove that the hydrophilic surface of Janus membranes repairs perforated eardrums, and meanwhile the hydrophobic surface can avoid adhering to the inner ear tissue for reducing hearing loss. The Janus membranes have good prospects in the treatment of tympanic membrane perforation.
研究领域:
生物材料
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组织工程
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耳鼻喉科
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Biomaterials Science & Engineering
·
2024
·
IF 5.4
Zhechen Yuan,Bing Mei Teh,Xiaoling Liu,Ziqian Liu,Juntao Huang,Yi Hu,Chengchen Guo,Yi Shen
Tympanic membrane perforation (TMP) is prevalent in clinical settings. Patients with TMPs often suffer from infections caused by Staphylococcus aureus and Pseudomonas aeruginosa, leading to middle ear and external ear canal infections, which hinder eardrum healing. The objective of this study is to fabricate an enzyme-responsive antibacterial electrospun scaffold using poly(lactic-co-glycolic acid) and hyaluronic acid for the treatment of infected TMPs. The properties of the scaffold were characterized, including morphology, wettability, mechanical properties, degradation properties, antimicrobial properties, and biocompatibility. The results indicated that the fabricated scaffold had a core–shell structure and exhibited excellent mechanical properties, hydrophobicity, degradability, and cytocompatibility. Furthermore, in vitro bacterial tests and ex vivo investigations on eardrum infections suggested that this scaffold possesses hyaluronidase-responsive antibacterial properties. It may rapidly release antibiotics when exposed to the enzyme released by S. aureus and P. aeruginosa. These findings suggest that the scaffold has great potential for repairing TMPs with infections.
研究领域:
生物医学工程
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材料科学
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组织工程
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生物材料
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抗菌技术
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耳部感染
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伤口愈合
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）