INDUSTRIAL CROPS AND PRODUCTS
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2018
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IF 3.849
Shujie Ma,Leilei Fu,Siqi He,Xiaopeng Lu,Yuanyong Wu,Zhiqing Ma,Xing Zhang
The evaluation of herbicidal activity and the isolation of plant allelopathic substances can lead to the discovery of new herbicide. This study has confirmed that the ethanol extract of S. mukorossi leaves was inhibitory to growth of Avena fatua L. and Amaranthus retroflexus L. both in petri dish experiment and pot culture assay, with the fresh weight inhibition varied between 47.60% and 62.05% at a concentration of 40 g/L of the leaf extract, being comparable to that of a commercialized natural product herbicide pelargonic acid at a concentration of 4 g/L. Five herbicidal compounds, oleanolic acid (1) , lupeol (2) , d-pinitol (3) , hexadecanoic acid (4) and octadecanoic acid (5) were isolated from the most active fraction, petroleum ether extract. D-pinitol (3) showed the highest growth inhibitory activity against both the shoot and root of A. fatua , with the half maximal inhibitory concentration (IC 50 ) values of 53.61 and 56.43 μg/mL, respectively. Furthermore, oleanolic acid (1) and d-pinitol (3) also inhibited the growth of A. retroflexus with the IC 50 value of 56.05 and 61.89 μg/mL against shoot growth, and 58.07 and 66.70 μg/mL against root growth, respectively. Thus, oleanolic acid (1) and d-pinitol (3) are the main phytotoxin constituents of S. mukorossi , and these compounds have the potential for further development as a botanical herbicide.
研究领域:
植物生理学
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化感作用
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天然产物化学
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杂草科学
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农药学
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2026
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IF 6.1
Qiaomei Lu,Le Huang,Haiyang Qiu,Yahui Shangguan,Wenmin Zhang,Yanjian Chen,Qinying Liu,Li Chen,Lan Zhang
Background Microporous organic network (MON) is a novel type of adsorbent and has wide applications in sample pretreatment. However, MON has a rigid plane and exhibits adsorption with most organic compounds, so they suffer from poor selectivity. The lack of selectivity hinders its practical application in complex matrices. Results This study focuses on enhancing the selectivity of MON by constructing a molecularly imprinted polymer (MIP) layer on its surface. A ganoderic acid molecularly imprinted magnetic microporous organic network composite (MMON@OA-MAA) was synthesized for the enrichment and separation of ganoderic acid (GD). The prepared MMON@OA-MAA material featured two key advantages: a high adsorption capacity (14.38-22.94 mg g −1 ) and excellent selectivity (α ≥ 1.5). The composite enabled hydrogen bonding, π-π interaction, and host-guest interactions with GD, which possessed active sites such as steroidal rings, hydroxyl groups, and carbonyl groups. An UPLC-MS/MS method was established and successfully applied to the efficient analysis of four trace GDs in complex Ganoderma samples, demonstrating a good linearity (r ≥ 0.9991), a low limit of detection (5.0 pg mL −1 ) and satisfactory recovery (81.0%-119.8%). Significance This research was a novel attempt to combine MIP with MMON to form MMON-MIP composite, achieving the synergistic enhancement of high selectivity and large adsorption capacity. The extraction process was simple and rapid, facilitating its broader application in the field of sample pretreatment, particularly for efficient analysis of trace bioactive components in complex natural matrices.
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JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2026
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IF 5.3
Huan Liu,Liying Liang,Minggang Wang,Qinfeng Huang,Yu Pan,Xiaojie Wei,Wenchao Zhang
The naturally occurring pentacyclic triterpenoid compounds ursolic acid (UA) and oleanolic acid (OA) are commonly found together in various fruits and find broad applications in pharmaceuticals, cosmetics, food and agricultural fields. In this study, a sensitive and practical icELISA method was developed based on a monoclonal antibody (mAb) YC23-101, with a median inhibitory concentration (IC 50 ) of 15.56 ng/mL and a linear range of 5.26 to 54.1 ng/mL. The limits of detection (LOD) ranges from 0.98-5.11 ng/mL in fruit matrices and the recoveries were 94.03-112.21% with coefficients of variation below 5.73%. Cross-reactivity analysis showed that mAb YC23-101 could also specifically recognize OA with similar sensitivity to UA, indicating feasibility for the simultaneously quantification of UA and OA in fruits. The developed icELISA was applied to several fruits, and the results showed good correlation with that of HPLC. Molecular docking analysis predicted equal binding energy of UA and OA to the mAb with hydrophobic interactions playing a predominant role. All these findings demonstrated that this icELISA is reliable, sensitive, and suitable for the high-throughput quantification of total UA and OA content in fruits composition analysis.
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CHEMICAL ENGINEERING JOURNAL
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2026
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IF 12.5
Yujie Xiao,Xianda Liu,Ziyue Ling,Shifan Chen,Ran Wei,Weifeng Zhao,Changsheng Zhao
Low-density lipoprotein apheresis (LDL-a) mainly aims to eliminate circulating low-density lipoprotein (LDL) in patients with atherosclerosis, but seldom alleviates the inflammatory responses that drive disease progression. Since the LDL provides more binding sites for acrylated β-cyclodextrin (AβCD) than oleanolic acid (OA), we propose that LDL could displace AβCD-bound OA, enabling LDL-responsive OA release and simultaneous LDL clearance. Herein, a dual-responsive LDL microsphere adsorbent with inflammation-regulating capability is successfully developed. By combining AβCD with the thermo-responsive poly(N-acryloylglycinamide) (PNAGA), the microspheres enable OA release triggered by both elevated LDL concentrations and temperature. The preloaded OA helps reduce nonspecific interactions between the microspheres and other serum proteins, without compromising the intrinsic LDL adsorption capacity of the microspheres. In normal saline, the elevated LDL concentration and temperature both trigger the release of OA from the microspheres, reflecting their dual-responsive behavior. In plasma, the LDL adsorption capacity of the microspheres reaches 76.9 mg/g. Under dynamic whole blood conditions, this value further increases to 184.6 mg/g. To elucidate the underlying mechanism, the isothermal titration calorimetry (ITC) results reveal that the binding constant (Ka) between AβCD and LDL is significantly higher than that between AβCD and OA. Moreover, the lower Gibbs free energy (ΔG) of AβCD-LDL indicates a more stable interaction. The anti-inflammatory potential of the microspheres is verified through co-incubation with RAW 264.7 macrophages, followed by qualitative immunofluorescence staining and quantitative ELISA analysis of inflammatory cytokines. Subsequently, the in vivo LDL clearance efficacy and biosafety of OA/NDSCD microspheres are validated using the rat hyperlipidemic model. This study demonstrates that the dual-responsive microspheres effectively integrate LDL removal and inflammation modulation, offering a promising approach to atherosclerosis.
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JOURNAL OF LIPOSOME RESEARCH
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2025
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IF 4.3
Amna Naz,Sadia Haider,Malik Ihsan Ullah Khan,Rabia Khokhar,Mavra Rubab,Muhammad Farooq,Attia Afzal,Muhammad Sarfraz
This study addresses the limitations of traditional cancer therapies—such as high toxicity and non-selectivity—by developing a novel nanoparticulate system co-loaded with plant-derived compounds, apigenin (APG) and oleanolic acid (OA). These naturally occurring phytochemicals are known for their anticancer properties but face clinical challenges due to poor solubility and limited efficacy. The Apigenin and Oleanolic Acid Nanoparticulate System (AONS) was refined and optimized using the Box-Behnken statistical design approach, resulting in nanoparticles with a mean diameter of 169 nm and size homogeneity. A validated HPLC technique was employed to simultaneously quantify both apigenin and oleanolic acid in the developed formulation as well as in various biological matrices. In vitro drug release was enhanced at acidic pH (5.5), mimicking the tumor microenvironment. AONS showed strong physicochemical stability under refrigerated storage. Cytotoxicity tests on HepG2, MDA-MB-231, and U87 glioma cells demonstrated significantly improved anticancer activity compared to control, free drugs, or individual nanoformulations. Cellular uptake studies confirmed efficient internalization in U87 cells, and ELISA results indicated apoptosis via activation of the p53 pathway. Biodistribution analysis revealed prolonged systemic drug retention with AONS (up to 72 hours), surpassing the short circulation time of free drugs. Notably, LC-MS data confirmed that the nanoparticle system could cross the blood-brain barrier, highlighting its therapeutic potential for treating brain cancers.
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PubMed
高影响因子
Food Bioscience
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2023
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IF 5.2
Man Ding,Xuechen Zhang,Jiangying Shi,Kaili Cui,Ruipeng Yang,Fengming Liu,Shuhua Shan,Ghani Israr,Zhuoyu Li
Colorectal cancer (CRC) is one of the most common malignant tumors worldwide, occurring in the colon or rectum portion. Quinoa is a dicotyledonous plant, and the quinoa bran is the outer seed coat of quinoa. Functional molecules in plants play an important role in the treatment and alleviation of chronic diseases. In this study, quinoa bran was used as the raw material. The active components with an anti-colorectal cancer effect were extracted by acetone and separated with an AB-8 macroporous resin column, which was further identified as terpenoids by liquid-mass spectrometry (LMS), named Quinoa bran terpenoids (QBT). The yield of quinoa bran terpenoids was 0.77%. Moreover, the results showed that QBT significantly inhibited the proliferation of colorectal cancer cells DLD-1 and HCT-8 with a concentration gradient dependent manner. Respectively, the IC 50 values of DLD-1 and HCT-8 cells were 0.42 ± 0.02 and 0.54 ± 0.05 mg/mL. Further studies showed that QBT upregulated apoptosis-related proteins such as activated-caspase-3, 8, and 9. In addition, QBT decreased mitochondrial membrane potential , promoted the expression of the pro-apoptotic protein Bax, and inhibited the anti-apoptotic protein Bcl-2. The experiments showed that QBT significantly repressed the growth of tumors in nude mice . In conclusion, these results indicated that QBT largely inhibits the development of colorectal cancer to large extent through upregulation of caspase-3, 8, and 9 and reduction of mitochondrial membrane potential, and has great potential for anti-tumor progression.
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高影响因子
FOOD RESEARCH INTERNATIONAL
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2023
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IF 8.1
Qiang Lyu,Wanying Zheng,Qiyuan Shan,Lichuang Huang,Yiwen Wang,Lu Wang,Haodan Kuang,Muhammad Azam,Gang Cao
Chemical structural characterization of chemical compounds from hawthorn fruits and its thermal processed products was carried out in present study. By linking Global Natural Products Social (GNPS) Molecular Networking and MolNetEnhancer workflow, seventy-four chemical compounds in hawthorn fruits and its thermal processed products were tentatively identified. Three quercetagetin derivatives (quercetagetin-3- O -glucoside, quercetagetin-di-glucoside and its isomer), five quercetin or kaempferol derivatives (quercetin-acetylapiosyl-hexoside, quercetin-3- O -(6″-malonyl-hexoside), quercetin-3- O -(6″-malonyl-hexoside)-(1 → 2)- O -hexoside, quercetin-3- O -(6″-malonyl-hexoside)-(1 → 2)- O -deoxyhexoside, kaempferol-3- O -(6″-malonyl-hexoside)), six procyanidins including four (E)C-ethyl-procyanidins and two A-type procyanidins digallate, as well as 13 triterpenoids including ursolic aldehyde, triterpenoid glycosides, and triterpene acids were reported for the first time in hawthorn fruits. In addition, triterpenoids exhibited considerable thermal stability, while all of flavonoid glycosides, proanthocyanidins and 10 in 13 organic acids showed dramatic decrease after thermal processing.
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PubMed
高影响因子
Small
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2022
·Vol. 19
·期 9
·2205531
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IF 15.153
Qian-Wei Luo,Lu Yao,Ling Li,Zhuo Yang,Mei-Mei Zhao,Yong-Zhe Zheng,Fang-Fang Zhuo,Ting-Ting Liu,Xiao-Wen Zhang,Dan Liu,Peng-Fei Tu,Ke-Wu Zeng
Understanding the direct interaction of nanostructures per se with biological systems is important for biomedical applications. However, whether nanostructures regulate biological systems by targeting specific cellular proteins remains largely unknown. In the present work, self-assembling nanomicelles are constructed using small-molecule oleanolic acid (OA) as a molecular template. Unexpectedly, without modifications by functional ligands, OA nanomicelles significantly activate cellular proteasome function by directly binding to 20S proteasome subunit alpha 6 (PSMA6). Mechanism study reveals that OA nanomicelles interact with PSMA6 to dynamically modulate its N-terminal domain conformation change, thereby controlling the entry of proteins into 20S proteasome. Subsequently, OA nanomicelles accelerate the degradation of several crucial proteins, thus potently driving cancer cell pyroptosis. For translational medicine, OA nanomicelles exhibit a significant anticancer potential in tumor-bearing mouse models and stimulate immune cell infiltration. Collectively, this proof-of-concept study advances the mechanical understanding of nanostructure-guided biological effects via their inherent capacity to activate proteasome.
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PubMed
高影响因子
TALANTA
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2023
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IF 6.1
Ying Wang,Xiaodan Wu,Guansong Shao,Bowen Zhai,Zihan Wang,Bingyang Qin,Tao Wang,Zhiguo Liu,Yujie Fu
An oleanolic acid (OA) surface molecularly imprinted polymer silylated porous composite aerogels (OA-MIP@Si-PC-aerogels) adsorbent material was successfully prepared and characterized. The material not only has a great selectivity for the target molecule OA but also has other noteworthy qualities including high stability, excellent repeatability, and a sizable adsorption capacity. via cellulose and sodium alginate as the main materials, the carrier Si-PC-aerogels were made through ionic cross-linking, chemical cross-linking, and silylation procedures. By adopting a surface molecular imprinting approach on Si-PC-aerogels, OA-MIP@Si-PC-aerogels were effectively created utilizing OA as the template molecule and MAA as the functional monomer . Due to the presence of a specific imprinted layer on the aerogel surface, the adsorption capacity of OA-MIP@Si-PC-aerogels for OA could reach 66.20 mg g −1 . OA-MIP@Si-PC-aerogels could achieve a 68.86% yield of OA from the extracts of lingonberry (Vaccinium Vitis-Idaea L .). The adsorption capacity remained at 90% after five consecutive adsorption-desorption cycles. HepG2 cells were exposed to OA that was effectively enriched with OA-MIP@Si-PC-aerogels in lingonberry (Vaccinium Vitis-Idaea L .) fruit homogenates. This OA significantly inhibited the growth of HepG2 cells in vitro. It further demonstrated that OA-MIP@Si-PC-aerogels could efficiently target OA enrichment and separation with good recovery.
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PubMed
高影响因子
BRITISH JOURNAL OF PHARMACOLOGY
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2023
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IF 7.3
Luyao Ma,Fangjun Yu,Di He,Lianxia Guo,Yu Yang,Wangchun Li,Tianpeng Zhang
Background and purpose The role of CLOCK in regulation of drug chronoefficacy and chronotoxicity remains elusive. Here, we aimed to uncover the impact of CLOCK and dosing time on clopidogrel efficacy and toxicity. Experimental Approach The antiplatelet effect, toxicity and pharmacokinetics experiments were conducted with Clock −/− and wild-type mice after gavage administration of clopidogrel at different circadian time points. The expression levels of drug metabolizing enzymes were determined by qPCR and Western blotting. Transcriptional gene regulation was investigated using luciferase reporter and chromatin immunoprecipitation assays. Key Results The antiplatelet effect and toxicity of clopidogrel in wild-type mice showed a dosing-time dependent variation. Clock ablation reduced the antiplatelet effect of clopidogrel, increased clopidogrel-induced hepatotoxicity, and blunted their rhythms. This was associated with attenuated rhythms of clopidogrel active metabolite (Clop-AM) and clopidogrel, respectively. We further found that Clock regulated the diurnal variation of Clop-AM formation by modulating the rhythmic expression of CYP1A2 and CYP3A11, and altered clopidogrel chronopharmacokinetics by circadian regulation of CES1D expression. Mechanistic studies revealed that CLOCK activated Cyp1a2 and Ces1d transcription by directly binding to the E-box elements in their promoters, and promoted Cyp3a11 transcription through enhancing the transactivation activity of DBP and TEF. Conclusion and implication CLOCK regulates the diurnal rhythmicity in clopidogrel efficacy and toxicity through regulation of CYP1A2, CYP3A11 and CES1D expression. The findings may contribute to the optimization of dosing schedule for clopidogrel, and deepen understanding of the connection between the circadian clock and chronopharmacology.
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PubMed
高影响因子
JOURNAL OF INORGANIC BIOCHEMISTRY
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2022
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IF 4.336
Mengdi Lv,Xiaoting Qian,Shijie Li,Jie Gong,Qun Wang,Yong Qian,Zhi Su,Xuling Xue,Hong-Ke Liu
It is a major challenge to design novel multifunctional metal-based chemotherapeutic agents for anti-tumor and anti-metastasis applications. Two complexes ( OA-Ir and OA-Ru ) were synthesized via CuAAC (copper-catalyzed azide-alkyne cycloaddition) reaction from nontoxic Ir - N 3 or Ru - N 3 species and low toxic alkynyl precursor OA-Alkyne, and exhibited satisfactory anti-tumor and anti-metastasis pharmacological effects . Conjugation of Oleanolic acid (OA) and metal-arene species significantly enhanced the cytotoxicity in A2780 cells compared to the precursors through mitochondrial-induced autophagy pathway. Moreover, the two complexes could inhibit the cell metastasis and invasion through damage of actin dynamics and down-regulation of MMP2/MMP9 proteins. Combination of two precursors improved the lipophilicity and biocompatibility, simultaneously enhanced the cell uptake and the mitochondrial accumulation of metal-arene complexes, which caused mitochondrial membrane potential damage, oxidative phosphorylation , ATP depletion and autophagy. Besides, OA-Ir and OA-Ru displayed excellent activity to disintegrate the 3D multicellular tumor spheroids , showing potential for the treatment of solid tumors. This work provides a new way for developing novel metal-based complexes via CuAAC reaction for simultaneously inhibiting tumor proliferation and metastasis.
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PubMed
高影响因子
Fermentation-Basel
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2023
·Vol. 9
·期 5
·432
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IF 3.7
Yuhao Sun,Zhenzhen Wang,Jing Dai,Ruyi Sha,Jianwei Mao,Yangchen Mao,Yanli Cui
In this work, spontaneously fermented and inoculation-fermentedAkebia trifoliatafruit Jiaosu (SFAJ/IFAJ) were compared. The key metabolites and antioxidant activities of SFAJ and IFAJ were tracked and tested during fermentation. The antioxidant effect of fermentedAkebia trifoliatafruit and the underlying mechanisms were explored using network pharmacology for the prediction and verification of the molecular targets and pathways of theAkebia trifoliatafruit’s action against oxidative stress. Furthermore, the results were verified by molecular docking and then investigated, based on a HepG2 cell model. The results of correlation analysis and principal component analysis (PCA) showed that there were significant positive correlations between the phenols, flavonoids, and terpenoids in SFAJ and IFAJ and their antioxidant activities. Network pharmacology and molecular docking analysis disclosed the antioxidation mechanism at the molecular level. In addition, both SFAJ and IFAJ were effective at alleviating oxidative stress in HepG2 cells. In particular, IFAJ performed better than SFAJ in protecting cells with an intracellular reactive oxygen species (ROS) level of 99.96 ± 4.07 U/mg prot, superoxide dismutase (SOD) activity of 41.56 ± 0.06 U/mg prot, catalase (CAT) activity of 91.78 ± 3.85 U/mg prot, and glutathione peroxidase (GSH-Px) activity of 39.32 ± 2.75 mU/mg prot in the IFAJ group. Collectively, this study revealed the changes in bioactive metabolite contents and the in vitro antioxidant activity during fermentation and investigated the protectiveness of SFAJ and IFAJ against oxidative stress within HepG2 cells, promoting the study of the antioxidant efficacy of IFAJ, thereby providing valuable reference data for the optimization of its preparation and the development of relevant products with health benefits.Keywords:Akebia trifoliatafruit;bioactive metabolites;antioxidant activity;network pharmacology;molecular docking;oxidative stress
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危险品化学品经营许可证（带存储）