PLANT GROWTH REGULATION
·
2025
·
IF 3.9
Liu Lin,Liu Liqin,Shi Shengyou,Shu Bo
Root senescence (RS) is a common phenomenon in longans; however, its regulation is poorly understood. In this study, the main root was divided into three segments from tip to crown, i.e., distal (MDR), middle (MMR) and proximal (MPR) parts, and lateral roots emerging from the corresponding segments of the main root, i.e., lateral roots from the distal (LDR), middle (LMR) and proximal (LPR) parts, were used for analysis of morphological characteristics, physiological root activity, transcriptome, proteome, and phytohormone quantification, in order to understand the regulation of RS in longan seedlings. The root hair and epidermis of the proximal parts of the main and lateral roots exhibited senescence, and activity decreased from the distal to the proximal parts, suggesting that both the main and lateral roots underwent senescence. The transcriptome profile showed that 2692 and 1406 differentially expressed genes were involved in the RS of the main and lateral roots, respectively, and the proteome data exhibited 222 and 124 differentially accumulated proteins involved in the RS of the main and lateral roots, respectively, based on the MPR vs. MMR and LPR vs. LMR groups. Based on the correlation analysis of DAPs and DEGs, the KEGG pathways of carotenoid biosynthesis (abscisic acid) and steroid biosynthesis (brassinosteroid) were found to be significantly different in the main roots, and two KEGG pathways related to plant hormones, phenylalanine metabolism (salicylic acid) and cysteine and methionine metabolism (ethylene), were significant in the lateral roots. Furthermore, the abscisic acid concentration in MMR was 9.47% lower than that in MPR and was negatively related to the root activity of the main root. The concentration of 1-aminocyclopropane-1-carboxylic acid increased 1.59 times from LDR to LPR and was negatively related to the activity of the lateral root. Thus, the results of this study indicate that different phytohormones are related to senescence regulation in the main and lateral roots of longan seedlings. In addition, transcriptome and proteome data provide basic information related to RS in longan seedlings.
研究领域:
植物学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2024
·Vol. 31
·期 25
·37316-37325
·
IF 0
Liu Yanping,Wang Xiaonan,Wang Siwei
Litchi and longan pests significantly affect crop yield and quality. Chemical prevention and control are very effective for production; therefore, it is crucial to study fate assessment and appropriate field efficacy before pesticide application on crops to appropriately assess the health and ecological risks linked with these agents. This study conducted Good Agricultural Practice (GAP) field trials and laboratory experiments to elucidate the dissipation, terminal residues, and efficacy of methoxyfenozide on litchi and longan in six locations throughout China. To detect methoxyfenozide residues on litchi and longan, a QuEChERS/UPLC-MS/MS-based method was designed. The initial methoxyfenozide levels in litchi and longan ranged from 2.21–2.86 to 0.83–0.95 mg kg −1 and indicated half-lives of 5.1–5.3 and 5.3–5.7 days, respectively. After 7 days of foliage treatment, the concentrations of terminal methoxyfenozide residue were 0.78–2.61 and 0.02–1.01 mg kg −1 , which were less than the established maximum residue limit for methoxyfenozide in litchi and longan. The chronic (acceptable daily intake = 0.0055–0.0331%) dietary intake risk analysis for methoxyfenozide in longan and litchi indicated acceptable concentrations of terminal residue for the general population. Methoxyfenozide in litchi and longan was readily degraded in first-order kinetics models, the degradation rate on longan was higher than that on litchi, and their dietary risks were negligible to consumers. Two hundred forty grams per liter of methoxyfenozide suspension concentrate (SC) represents a highly efficacious insecticidal dose to control litchi and longan pests and indicates a significant application potential as it is rapidly degraded and linked with reduced post-treatment residue levels.
研究领域:
农业科学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Ya-Hui Yu,Kouadio Jean Eric-Parfait Kouame,Xiaozhen Liu,Xiangying Yu,Ming-Yu Jin,Long-Qing Li,Fengyuan Liu,Yuting Li,Jing-Kun Yan,Bing Li
The combination of selenium nanoparticles (SeNPs) with natural polysaccharides represents an effective strategy to address the limitations of SeNPs and providing a stable, highly bioactive form of Se. In this study, we explored the potential of longan polysaccharides (LP) in the preparation and stabilization of SeNPs. The morphology, stability, stabilization mechanism, and cytotoxicity of LP-SeNPs were investigated. The results showed that the LP-SeNPs were uniform spherical nanoparticles with a mean particle size of 100.4 ± 1.1 nm, and demonstrated excellent stability over a 14-day storage period, which was attributed to the interaction between the SeNPs and the hydroxyl groups present on the surface of LP. Meanwhile, LP-SeNPs have no toxic effect on rat intestinal epithelial (IEC-6) cells, while they are cytotoxic to human colon cancer (HCT-116 and HT-29) cells, including growth inhibition, morphological alteration, and the promotion of reactive oxygen species and mitochondrial membrane potential loss in a dose-dependent manner. LP-SeNPs induced apoptosis in HCT-116 and HT-29 cells by regulation of the apoptosis-related proteins including Bax, Bcl-2, caspase-3, and cytochrome c . Therefore, our results provide a theoretical basis for the development and application of LP and indicate the potential promise of LP-SeNPs as dietary Se supplements and health-promoting food ingredients.
研究领域:
食品科学
·
生物医学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Yang Xiao,Nan Chen,Qiang He,Wei-Cai Zeng
In the present study, the preparation of resistant starch from longan seeds was investigated, and the properties and in-vitro digestibility of this resistant starch were further determined. Longan seed resistant starch (LRS I, LRS-II, and LRS-III) was successfully prepared, by once retrogradation, twice retrogradation and combining retrogradation and debranching, respectively. All LRS groups had higher amylose content (from 422.1 to 462.4 g/kg) and increased particle size than those of longan seed starch (LSS). Meanwhile, the resistant starch exhibited a B-type crystalline structure, and showed a block, uneven and irregular microstructure. Compared with LSS group, LRS groups exhibited higher crystallinity (from 49.03 to 56.14 %), more of short-range ordered structure (DO: from 0.771 to 0.775, DD: 0.808 to 0.942), and lower molecular weight (Mw: from 2.43 to 2.81 × 10 5 Da). Moreover, LRS groups showed lower viscosity during gelatinization and weaker binding capability to iodine than those of LSS group. Furthermore, all LRS groups exhibited strong resistance to enzymatic hydrolysis with high content of resistant starch (from 360 to 398 g/kg) and low rate of in-vitro digestion ( k : from 23.87 to 33.74, C ∞ : from 63.5 to 65.5). All present results suggest that longan seeds show the potential to prepare resistant starch applied in food industry.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Weiming Chai,Wenshuang Wei,Xinru Hu,Qiuhan Bai,Yiwen Guo,Mingyi Zhang,Shuting Li,Qiuxia Pan
Tyrosinase is a biological macromolecule closely related to browning of fruit and vegetables, melanin production , and tyrosinase inhibitors are usually used to prevent browning and pigmentation . In this study, longan shell tannins (LSTs) were screened as tyrosinase inhibitors and their structures were proved to be mixtures of procyanidins (condensed tannins) and ellagitannins (hydrolyzed tannins). Enzymatic experiments verified that LSTs were efficient inhibitors, and the IC 50 values for monophenolase and bisphenolase were 176.04 ± 10 and 59.94 ± 5 μg mL −1 , respectively. Fluorescence detections and molecular docking revealed that the combination of LSTs to tyrosinase was mainly driven by hydrogen bonding, hydrophobic interaction, as well as van der Waals force , which changed the microenvironment of tyrosine and tryptophan residues as well as enzyme conformation. Circular dichroism and molecular dynamics simulation showed that LSTs affected secondary structures of tyrosinase, resulting in structural stretching and conformational modification of the enzyme. In addition, preservation studies demonstrated that LSTs owned the ability to delay the browning of fresh-cut apples by inhibiting phenolic metabolism, strengthening the antioxidant system, and reducing lipid peroxidation . This paper testified that LSTs are exteaordinary tyrosinase inhibitors, and offered a scientific foundation for the application of LSTs in food industry and medicine.
研究领域:
食品科学
·
植物化学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Hongkai Huang,Jiahao Wang,Yuqing Zheng,Weidong Bai,Ya Ma,Xiaojuan Zhao
The discarded longan shell-derived porous carbon material (LPC) served as a scaffold for synthesizing bismuth nanoparticle-loaded longan porous carbon nanocomposite (BiNPs@LPC) via a hydrothermal method . Then BiNPs@LPC was utilized to modify screen-printed carbon electrodes (SPCE) for simultaneous detection of Pb(II) and Cd(II) by square wave anodic stripping voltammetry (SWASV). The material was thoroughly characterized by scanning electron microscopy , X-ray diffraction, Raman spectra , Brunauer-Emmett-Teller analysis, electrochemical impedance spectroscopy and cyclic voltammetry . BiNPs@LPC exhibited abundant porous structures, high surface area , and numerous active sites, which could improve significantly response sensitivity. Under optimal conditions, the peak currents of Pb(II) and Cd(II) exhibited favorable linear relationships with the concentration within a range of 0.1–150 μg L −1 , with detection limits (S/N = 3) of 0.02 μg L −1 and 0.03 μg L −1 , respectively. BiNPs@LPC/SPCE demonstrated remarkable selectivity , stability and repeatability. The proposed method was successfully applied for the detection of Pb(II) and Cd(II) in seafoods achieving satisfying recovery of 97.8%–108.3% and 96.7%–106.4%. These excellent test properties were coupled with convenience for batch preparation of the modified electrodes , highlighting its potential for practical applications in heavy metal detection of real samples.
研究领域:
环境科学
·
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2014
·
IF 3.065
Xiangnan Li,Jiale Huang,Zidan Wang,Xinde Jiang,Wencheng Yu,Yanmei Zheng,Qingbiao Li,Ning He
The alkaline extraction and acid precipitation of longan seed phenolic compounds were investigated to develop a more practical process than currently exists to isolate these chemicals. By using an alkaline buffer as the extraction solvent, the yield of total phenols reached 46.86±0.43 mg/g after conventional solid–liquid extraction. Because of the benefit achieved from the preconcentration process, an acid precipitation efficiency of 65.29 ± 0.38% was obtained, and the separation yield of phenols reached 22.04 ± 1.95 mg/g. However, the purity of the phenolic extract was influenced by the presence in the precipitate of proteins and carbohydrates, which both moved together in all four precipitation fractions at different pH values. The FRAP assay and the assay of the radical-scavenging activity revealed the strong antioxidant activity possessed by the phenolic compounds in the precipitate extracts. This preparation process for phenolic compounds has obvious advantages, such as easy operation, safe products and low production cost, which indicated its prospective industrial potential.
研究领域:
食品科学
·
植物化学
✓ 已验证
产品关联
DOI
高影响因子
Nutrients
·
2022
·Vol. 14
·期 9
·1710
·
IF 6.706
Ya-Hui Yu,Zhi-Mei Tang,Cen Xiong,Fei-Fei Wu,Jun-Ren Zhao,Xin-Huai Zhao
The selenylated polysaccharides chemically belong to the organic Se-conjugated macromolecules and have recently been attracting more and more attention due to their potential to promote body health or prevent cancers. Longan (Dimocarpus longanL.), as a subtropical fruit, contains soluble and non-digestible polysaccharides that are regarded with health care functions in the body. In this study, the longan polysaccharides (LP) were obtained via enzyme-assisted water extraction, and then chemically selenylated using a reaction system composed of HNO3–Na2SeO3to yield two selenylated products, namely, SeLP1 and SeLP2, with Se contents of 1.46 and 4.79 g/kg, respectively. The anti-cancer effects of the three polysaccharide samples (LP, SeLP1, and SeLP2) were thus investigated using the human colon cancer HT-29 cells as the cell model. The results showed that SeLP1 and SeLP2 were more able than LP to inhibit cell growth, alter cell morphology, cause mitochondrial membrane potential loss, increase intracellular reactive oxygen and [Ca2+]ilevels, and induce apoptosis via regulating the eight apoptosis-related genes and proteins including Bax, caspases-3/-8/-9, CHOP, cytochrome c, DR5, and Bcl-2. It was thereby proven that the selenylated polysaccharides could induce cell apoptosis via activating the death receptor, mitochondrial-dependent, and ER stress pathways. Collectively, both SeLP1 and SeLP2 showed higher activities than LP in HT-29 cells, while SeLP2 was consistently more active than SeLP1 in exerting these assessed anti-cancer effects on the cells. In conclusion, this chemical selenylation covalently introduced Se into the polysaccharide molecules and caused an enhancement in their anti-cancer functions in the cells, while higher selenylation extent was beneficial to the activity enhancement of the selenylated products.Keywords:longan polysaccharides;selenylation;HT-29 cells;anti-cancer activity;apoptosis
研究领域:
药理学
·
癌症研究
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2018
·Vol. 19
·期 8
·2403
·
IF 3.687
Yukun Chen,Xiaoping Xu,Xiaohui Chen,Yan Chen,Zihao Zhang,Xu Xuhan,Yuling Lin,Zhongxiong Lai
Mother of FT and TFL1(MFT) belongs to phosphatidylethanolamine-binding protein (PEBP) family, which plays an important role in flowering time regulation, seed development, and germination. To gain insight into the molecular function ofDlMFTinDimocarpus longanLour., we isolatedDlMFTand its promoter sequence from longan embryogenic callus (EC). Bioinformatic analysis indicated that the promoter contained multiphytohormones and light responsive regulatory elements. Subcellular localization showed that the given the DlMFT signal localized in the nucleus, expression profiling implied thatDlMFTshowed significant upregulation during somatic embryogenesis (SE) and zygotic embryogenesis (ZE), and particular highly expressed in late or maturation stages. The accumulation ofDlMFTwas mainly detected in mature fruit and seed, while it was undetected in abortive seeds, and notably decreased during seed germination.DlMFTresponded differentially to exogenous hormones in longan EC. Auxins, salicylic acid (SA) and methyl jasmonate (MeJa) suppressed its expression, however, abscisic acid (ABA), brassinosteroids (BR) showed the opposite function. Meanwhile,DlMFTdifferentially responded to various abiotic stresses. Our study revealed thatDlMFTmight be a key regulator of longan somatic and zygotic embryo development, and in seed germination, it is involved in complex plant hormones and abiotic stress signaling pathways.Keywords:mother of FT and TFL1;longan;embryogenesis;phytohormones;stress response;quantitative real-time PCR
研究领域:
植物生物学
·
分子遗传学
·
发育生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 17
·5737
·
IF 4.927
Siwei Wang,Xiaonan Wang,Qiang He,Haidan Lin,Hong Chang,Haibin Sun,Yanping Liu
This study established a QuEChERS high-performance liquid chromatography/tandem triple-quadrupole mass spectrometry method for determining azoxystrobin, pyraclostrobin, picoxystrobin, difenoconazole, chlorantraniliprole, imidacloprid, and cyantraniliprole and its metabolite (IN-J9Z38) in litchi and longan, and applied this method to the real samples. The residues in samples were extracted with acetonitrile and purified with nano-ZrO2, C18, and PSA. The samples were then detected with multireactive ion monitoring and electrospray ionization in the positive ion mode and quantified using the external matrix-matched standard method. The results showed good linearities for the eight analytes in the range of 1–100 μg/L, with correlation coefficients (r2) of >0.99. The limit of quantification was 1–10 μg/kg, and the limit of detection was 0.3–3 μg/kg. Average recovery from litchi and longan was 81–99%, with the relative standard deviation of 3.5–8.4% at fortified concentrations of 1, 10, and 100 μg/kg. The developed method is simple, rapid, efficient, and sensitive. It allowed the rapid screening, monitoring, and confirming of the aforementioned seven pesticides and a metabolite in litchi and longan.Keywords:QuEChERS;HPLC-MS/MS;litchi and longan;pesticide and metabolite;residue
研究领域:
分析化学
·
食品安全
·
植物保护
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Plants-Basel
·
2024
·Vol. 13
·期 21
·3061
·
IF 4
Rob Md Mahfuzur,Dongmei Han,Jianhang Xu,Yuqiong Lin,Xiaomeng Guo,Tao Luo,Zhenxian Wu,Shilian Huang,Xinmin Lv,Junbin Wei
SO 2 fumigation treatment (commonly known as sulfur treatment, ST) is a key method in the postharvest preservation of imported and exported fresh longan fruits, effectively reducing pericarp browning and enhancing color. Nonetheless, distinctive aromas, often referred to as “sulfur flavor”, may develop in the aril during the extended preservation period. This study employed “Caopu” longan as the test material and patented SO 2 -releasing paper (ZL201610227848.7) as a treatment to perform a 35-day low-temperature (5 °C) storage of the fruit. The changes in glucosinolates (GSLs) and associated metabolites in the aril of treated fruit (ST) were examined utilizing ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) detection and widely targeted metabolomics technology. The findings indicated that following 35 days of storage, nearly all control (CK) fruit pericarp turned to brown, resulting in an edible fruit rate of 75.41% and a commercial fruit rate of 0%. In contrast, the treated (ST) fruit demonstrated an edible fruit rate and a commercial rate of 99.44%, while the pericarp color changed from dark yellow-brown to light earthy yellow. The sulfur-containing metabolites identified in longan fruit aril predominantly consist of amino acids and their derivatives (60.44%), followed by alkaloids (15.38%), nucleotides and their derivatives (1.10%), and other types (23.08%), which include GSLs. SO 2 treatment significantly reduced the content of oxidized glutathione in fruit aril but increased the content of GSLs and related amino acids and their derivatives. Via screening, 19 differential sulfur-containing metabolites were obtained between ST and CK, including 11 GSLs. The identified differential metabolites of GSLs were all increased, primarily comprising aliphatic GSLs, such as 1-hydroxymethyl glucosinolate, 2-Propenyl glucosinolate (Sinigrin), and 4-Methylsulfinylbutyl glucosinolate (Glucoraphanin). Pathway analysis showed that these differential metabolites were mainly involved in coenzyme factor synthesis, cysteine and methionine metabolism, and amino acid synthesis, among other pathways. To the best of our knowledge, this is the first study to reveal the causes of the special flavor of longan aril after SO 2 treatment, which is a great concern for longan consumers. Moreover, this study provides a scientific basis for exploring the reasons and mechanisms for the development of the sulfur flavor in the SO 2 -treated fruits during postharvest storage.
研究领域:
食品科学
·
采后技术
·
代谢组学
·
植物生理学
✓ 已验证
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Weiming Chai,Tingting Shao,Shanmei Zeng,Yiwen Guo,Yifeng Wu,Wenshuang Wei,Qiuxia Pan,Xiao-Lei Xia
Longan seed tannins (LSTs) were separated and confirmed to be mixtures of procyanidins, ellagitannins, and gallotannins. Multiple spectroscopy methods and molecular simulation technique demonstrated that LSTs were competitive inhibitors of tyrosinase and formed stable complex with the enzyme. The LSTs influenced the micro-environment of amino acid residues in the catalytic center of tyrosinase by non-covalent interactions, and induced stretching of polypeptide framework and conformational change of the enzyme by altering its secondary structures, generating an intense inhibition in enzyme activity. Moreover, LSTs effectively prevented the enzymatic browning of apples by inhibiting the polyphenol oxidation, enhancing the anti-oxidation system, and preventing the lipid peroxidation of cell membrane. This study demonstrated that LSTs were quality tyrosinase inhibitors and browning resistance agents, and provided novel insights for understanding the suppressive mechanism of LSTs on tyrosinase and browning, as well as scientific evidence for their potential application as food preservative.
研究领域:
食品科学
·
植物化学
·
酶学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）