Foods
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2025
·Vol. 14
·期 13
·2370
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IF 5.1
Shuyuan Chen,Weiyun Guo,Tonghe Zhang,Jianfang Chen,Li Huang,Jihong Huang,Ruqiang Huang
Solanum nigrumL., a widely consumed Asian medicinal edible plant, is a promising source of bioactive saponins for functional food applications. This study optimized the extraction of saponins fromS. nigrumfruits (8.59% total saponin yield), followed by isolation via column chromatography and structural elucidation using spectroscopic analyses (IR, NMR, and MS). Concurrently, the antioxidant properties and antibacterial activity of the purified substances were detected and analyzed. The three saponins (SNL1, SNL2, SNL3) were identified as γ2-Solamargine , Diosgenin, and β-Solanine. Then-butanol -purified fraction demonstrated a remarkable capacity to scavenge DPPH, hydroxyl, and ABTS radicals (DPPH IC50 = 0.0096 mg/mL; hydroxyl radical IC50 = 0.8 mg/mL; ABTS IC50 = 0.061 μg/mL), indicating the inhibition of a multi-pathway oxidative chain reaction. Concurrently, the saponins exhibited selective antimicrobial efficacy against key foodborne pathogens, particularly Escherichia coli. To the best of our knowledge, this work provides the first empirical evidence ofS. nigrumfruit saponins as dual-functional natural preservatives, synergistically suppressing lipid oxidation and microbial growth. These findings highlight their potential as safer, multi-mechanistic alternatives to synthetic additives, aligning with clean-label food industry demands.
研究领域:
食品科学
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植物化学
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微生物学
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药用植物学
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产品关联
DOI
PubMed
高影响因子
Results in Chemistry
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2025
·
IF 4.2
Yan Liu,Zhao Jiankan,Minzhen Si
This study employs Fourier Transform Infrared Spectroscopy (FTIR) to quantitatively analyze the distribution patterns and metabolic roles of α-solanine across different organs (roots, stems, leaves, flowers, and fruits) of Solanum nigrum . By extracting absorbance values from key characteristic peaks (2923 cm −1 , 2853 cm −1 , 1639 cm −1 , 1152 cm −1 , and 1072 cm −1 ) and applying a normalization ratio method for quantitative assessment, our results reveal that fruits serve as the primary accumulation site of α-solanine, followed by flowers and leaves, whereas the lowest concentrations are detected in roots and stems. Further analysis suggests that the high accumulation in fruits is likely associated with defensive functions and seed protection, while leaves and flowers function as the primary sites for α-solanine biosynthesis and signaling regulation, respectively. In contrast, roots and stems mainly facilitate transport and storage. This study highlights FTIR as a rapid, non-destructive technique for the quantitative analysis of plant secondary metabolites, demonstrating its potential in botanical research and biochemical profiling. Moreover, these findings provide valuable insights into the ecological roles of Solanaceae secondary metabolites and their potential applications in agriculture and pharmaceutical sciences.
研究领域:
植物学
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药理学
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分析化学
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产品关联
DOI
高影响因子
INTERNATIONAL IMMUNOPHARMACOLOGY
·
2025
·
IF 4.7
Wenwei Li,Yang Liu,Ming Wei,Zhichao Yang,Hao Tang,Wei Huang
α-Solanine, a glycoalkaloid (GA) extracted from the stems of the potato plant, exhibits bioactivity and medicinal potential that necessitate further investigation. The impact and underlying mechanisms of α-Solanine on osteoarthritis (OA) remain to be elucidated. To achieve enhanced therapeutic outcomes, we have designed and synthesized a UIO-66-NH 2 @α-Solanine@PEI charged particle (USP) that amplifies the therapeutic effects of α-Solanine, demonstrating superior efficacy. Our approach involved the synthesis of a novel drug delivery system, the USP, to augment the therapeutic potential of α-Solanine in the treatment of OA. An OA rat model was established, and USP treatment was administered. The therapeutic effects were verified through histochemical staining and micro-CT. In vitro, α-Solanine significantly suppressed the expression of proteins related to glycolysis and notably inhibited ferroptosis. RNA sequencing revealed hypoxia-inducible factor-1α (HIF-1α) as a potential pathway mediating the effects of α-Solanine, and it was found that the co-addition of cycloheximide (CHX) led to a shortened decay time of HIF-1α. In vivo, rats with OA demonstrated significant inhibition of glycolysis and ferroptosis following treatment with USP, along with improvements in OA characteristics. These findings suggest that α-Solanine can inhibit the intense glycolysis associated with OA via the HIF-1α pathway and alleviate ferroptosis in chondrocytes. Treatment with USP demonstrated superior efficacy in the management of OA, providing a new therapeutic strategy for the disease.
研究领域:
医学
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药理学
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生物化学
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产品关联
DOI
PubMed
高影响因子
PHYTOCHEMICAL ANALYSIS
·
2023
·
IF 3.3
Qi Zhang,Dongmei Liu,Yanru Cui,Tongxin Xu,Tiantian Lu,Xiaoli Liu,Kun Liu,Quande Wang,Aiqian Li,Pengfei Zhao,Zhongzhe Cheng
Introduction Solanum nigrum L. is a traditional medicinal herb and edible plant. Many studies provide evidence that S. nigrum L. is a nutritious vegetable. Polyphenols and steroidal glycoalkaloids are the main components. Objectives This study aimed to systemically evaluate the phytochemical profile, quantification, and bioactivities of polyphenolics and glycoalkaloids in different parts of S. nigrum L. Results Total polyphenols (TPC) and total glycoalkaloids (TGK) were determined using the Folin–Ciocalteu and acid dye colorimetric methods, respectively. A total of 55 polyphenolic constituents (including 22 phenolic acids and 33 flavonoids) and 24 steroidal glycoalkaloids were identified from different parts using ultrahigh-performance liquid chromatography Q-exactive high-resolution mass spectrometry (UHPLC-QE-HRMS), of which 40 polyphenols (including 15 phenolic acids and 25 flavonoids) and one steroidal glycoalkaloid were characterised for the first time in S. nigrum L. Moreover, typical polyphenols and glycoalkaloids were determined using HPLC-UV and HPLC–evaporative light-scattering detector (ELSD), respectively. In addition, the TPC and TGK and their typical constituents were compared in different anatomical parts. Finally, the antioxidant capacities of polyphenolic extracts from different parts of S. nigrum L. were evaluated by ·OH, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and ferric-reducing antioxidant power (FRAP) assay in vitro . In addition, the antitumour effects of TGK from different parts of S. nigrum L. on the proliferation of PC-3 cells were investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Polyphenolic and glycoalkaloid extracts from different parts of S. nigrum L. showed different antioxidant and cytotoxic capacities in vitro . Conclusion This is the first study to systematically differentiate between polyphenolic and glycoalkaloid profiles from different parts of S. nigrum L.
研究领域:
药用植物学
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分析化学
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药理学
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植物科学
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Shuchen Pei,Kangli Yan,Shaoyu Cai,Hang Zou,Kang Luo,Yunqi Ma
This paper reports a one-step hydrothermal carbonation method for the synthesis of yellow-green carbon dots from Solanum nigrum L, with phosphoric acid as dopant. The phosphorus-doped carbon dots ( P-CDs) were characterized with TEM, XRD, FT-IR, XPS, and UV–Visible. P-CDs provided good fluorescence enhancement of ciprofloxacin with excellent selectivity and sensitivity, while maintaining robust performance against ionic interference and pH variations. The P-CDs demonstrated high sensitivity toward (ciprofloxacin) CIP, with a wide linear detection range of 0.01–0.1 μM and an ultralow detection limit of 6.37 × 10 −3 μM. Moreover, the P-CDs effectively inhibited S. aureus and E. coli growth at a MIC of 0.27 mg/mL. The P-CDs enable rapid antibiotic detection within 5 min while demonstrating potent antibacterial efficacy with a remarkably low propensity for inducing drug resistance, thereby opening new avenues for combating drug-resistant bacterial infections. Notably, the P-CDs offer a simple, rapid, and highly sensitive detection method for CIP analysis in milk samples, making them a promising tool for food safety monitoring. This finding will support the future development of carbon dots for food chemistry applications.
研究领域:
纳米技术
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食品安全
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分析化学
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微生物学
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材料科学
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产品关联
DOI
高影响因子
Scientific Reports
·
2025
·Vol. 15
·期 1
·1-10
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IF 3.9
Shen Fangzhou,Lu Zhaoyang,Yan Kangli,Luo Kang,Pei Shuchen,Xiang Pu
This work reports a novel one-pot hydrothermal synthesis of N-CQDs using Solanum nigrum L . as sustainable carbon source and triethylamine as nitrogen provider. The resulting N-CQDs were systematically characterized with TEM, SEM, FT-IR, XPS, XRD, and UV–Visible. Significantly, the N-CQDs exhibited substantial antimicrobial efficacy against both S. aureus and E. coli bacterial strains, with minimum inhibitory concentration values of 1.2 mg/mL and 1.1 mg/mL, respectively. Moreover, the N-CQDs demonstrated pronounced fluorescence enhancement upon interaction with ciprofloxacin, displaying exceptional selectivity and sensitivity, with a calculated LOD of 316 nM. The feasibility of employing N-CQDs as fluorescent probes for ciprofloxacin detection was systematically validated through practical application in honey analysis. This study introduces a novel approach for the fabrication of N-CQDs derived from natural botanical sources, underscoring their potential dual functionality as fluorescent sensors and antimicrobial materials.
研究领域:
材料科学
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纳米技术
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分析化学
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微生物学
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药学
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环境科学
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产品关联
DOI
PubMed
高影响因子
Plants-Basel
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2025
·Vol. 14
·期 18
·2918
·
IF 4.1
Guannan Kong,Da Song,Chao Zhang,Xinyao Jia,Yingying Ren,Shuhe Wei,Huiping Dai
Cadmium contamination in soil threatens ecological safety and human health. Phytoremediation has gained attention due to its cost-effectiveness and environmental sustainability. Studies show that plant growth-promoting rhizobacteria can enhance the ability of hyperaccumulator plants to remove heavy metals. This research aimed to isolate and identify plant-growth-promoting rhizobacteria under Cd stress and assess their impact on the growth and Cd accumulation ofSolanum nigrumL. Six bacterial strains were isolated from the rhizosphere ofS. nigrum, all showing high Cd tolerance. Among them, LKT25 exhibited multiple growth-promoting traits, including indole-3-acetic acid production, nitrogen fixation, 1-aminocyclopropane-1-carboxylate deaminase, and siderophore synthesis. Under varying Cd concentrations (5, 25, and 50 mg/kg), theBacillus thuringiensisstrain LKT25 significantly improved Cd removal byS. nigrum. At 5 mg/kg Cd, the removal efficiency reached 45.13%. LKT25 also enhanced plant growth, photosynthesis, and antioxidant activity, contributing to improved Cd remediation. This study provides new microbial resources and technical support for using rhizobacteria in remediating heavy metal-contaminated soils.
研究领域:
环境微生物学
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植物生理学
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土壤修复
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重金属污染治理
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环境生态学
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产品关联
DOI
PubMed
高影响因子
FASEB JOURNAL
·
2025
·Vol. 39
·期 16
·e70906
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IF 4.2
Lina Wei,Lei Wang,Yang Yu,Xiaozhou Sun,Liang Liu,Jing Han,Yongji Wang,Yinan Guo
Pediatric bacterial pneumonia continues to pose a significant global health burden, often resulting in serious complications. Recent studies have emphasized the pivotal role of the gut-lung axis in the progression of respiratory diseases, wherein disruptions in gut microbiota can aggravate pulmonary inflammation. This research investigates the underlying mechanisms by which Ma-Xing-Shi-Gan Decoction (MXSG), a classical formula in traditional Chinese medicine, influences the gut-lung axis in the context of childhood bacterial pneumonia. Clinical data indicated pronounced alterations in intestinal microbial composition and a marked decline in short-chain fatty acid (SCFA) levels among affected pediatric patients—changes that coincided with dysfunctional gut-lung signaling. In a murine model of bacterial pneumonia, MXSG administration restored microbial diversity, elevated SCFA production, and attenuated inflammatory responses by suppressing the HDAC3/NF-κB signaling cascade. Key bioactive constituents of MXSG—namely, quercetin, osajin, nobiletin, and solasodine—were identified as critical regulators, modulating lipid metabolism and promoting SCFA biosynthesis. These results highlight the therapeutic promise of MXSG in reestablishing gut-lung homeostasis and shed light on its mechanistic potential in the treatment of pediatric bacterial pneumonia. Graphical This schematic illustrates how MXSG restores gut microbiota diversity and SCFA levels, suppresses HDAC3/NF-κB signaling, and alleviates lung inflammation in pediatric bacterial pneumonia. Integrated multi-omics and mouse model experiments reveal that MXSG modulates the gut-lung axis and provides a novel mechanism for its clinical efficacy.
研究领域:
中医药学
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微生物学
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免疫学
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药理学
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分子生物学
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儿科学
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呼吸疾病
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）