ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Jijuan Zhou,Dianlong Ge,Yue Liu,Yajing Chu,Xiangxue Zheng,Yan Lu,Yannan Chu
Cellular volatile organic compound (VOC) detection is crucial for studying lung cancer biomarkers. However, the reported VOC biomarkers from the same cell line seem to be inconsistent across different research groups. It is possibly related to the variation in culture media, and the result obtained by a conventional single medium approach (SMA) depends on what medium is used in the cell experiment. This study proposes a multiple media approach (MMA) to investigate reproducible characteristic VOCs of lung cancer cells. Using solid-phase microextraction–gas chromatography–mass spectrometry (SPME-GC-MS) in combination with untargeted analysis, we conducted two independently repetitive experiments to compare lung cancer cells (A549) and normal lung cells (BEAS-2B) under three culture media conditions (RPMI 1640, DMEM, and Ham’s F12). Both experiments indicated that, compared with 62–96 VOCs obtained by the SMA, only two VOCs (3-methyl-1-butanol and 2-methyl-1-butanol) were reproducibly achieved by the MMA. Moreover, their concentrations were significantly lower in lung cancer cells than in normal cells. Further targeted analysis confirmed the downregulation trend of both VOCs in subcutaneous and primary tumor tissues from the lung cancer model mouse. The present work demonstrated that the MMA cell experiment, just like the multicenter trials for cell lines, can facilitate the discovery of reproducible characteristic VOCs. This provides a cellular experimental basis and scientific evidence for lung cancer biomarker investigation and even breath biopsy technique development.
研究领域:
医学
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分析化学
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肿瘤学
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DOI
PubMed
高影响因子
CRYSTENGCOMM
·
2016
·Vol. 18
·期 21
·3842-3849
·
IF 3.849
Chunping Gao,Qi Shi,Jinxiang Dong
The performance of two hydrophobic zeolitic imidazolate frameworks, MAF-6 and TIF-1 Zn, in adsorptive separation of 1-butanol from acetone–butanol–ethanol model fermentation broth was studied and compared to the data reported for ZIF-8. It was found that, in single-component systems, the adsorption capacities of ZIF-8 and MAF-6 were larger than that of TIF-1 Zn, mainly because of their larger pore volume; however, the time necessary to reach equilibrium was shorter when TIF-1 Zn with a larger pore aperture was used. In breakthrough experiments, it was shown that the adsorptive separation mechanism on ZIF-8 and MAF-6 was based on combined thermodynamics and kinetic effects; on TIF-1 Zn, thermodynamic adsorption took place.
研究领域:
生物工程
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化学工程
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DOI
高影响因子
COLLOID AND POLYMER SCIENCE
·
2021
·Vol. 299
·期 9
·1459-1468
·
IF 1.931
Guan Peiwen,Ren Cong,Shan Houchao,Cai Di,Zhao Peimian,Ma Dongze,Qin Peiyong,Li Shufeng,Si Zhihao
To meet the increasing requirements toward biobutanol, pervaporation membrane with high separation performance needs to be developed. In this work, a hydrophobic MAF-6 (RHO-[Zn(eim) 2 ]) was synthesized and its adsorption properties for 1-butanol was systematically studied, which are supposed to benefit the separation performance of polydimethylsiloxane (PDMS) membrane. The hydrophobic MAF-6 particles were therefore doped into PDMS to fabricate mixed matrix membrane. The morphology, chemical structure, and other properties of particles and hybrid membrane were fully studied by SEM, PXRD, FT-IR, TGA, and BET. Results demonstrate that the hydrophobicity of PDMS membrane is improved with the water contact angle increasing from 112.7° to 118.1° after being doped with MAF-6, as well as enhanced adsorption ability toward 1-butanol. More importantly, when doping MAF-6 into PDMS layer, the enhancements of 23.30% and 41.93% are separately observed in the separation factor and total flux to separate 1.5 wt% 1-butanol solution, compared with the PDMS membrane. In addition, the heat energy consumption of pervaporation process is decreased from 4.27 to 3.71 kJ g −1 via the doping of MAF-6. Overall, this work confirms that the hydrophobic particle such as MAF-6 is not only conductive to enhance the separation performance of PDMS membrane but also helpful to decrease the energy consumption.
研究领域:
材料科学
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化学工程
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产品关联
DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2014
·
IF 4.28
Xiuting Hu,Hongyan Li,Benxi Wei,Xueming Xu,Zhengyu Jin,Yaoqi Tian
The aim of this study was to examine 1-buatnol–hydrochloric acid (1-butanol–HCl) hydrolysis process of starch . Normal rice starch was hydrolyzed by 0.36% HCl in anhydrous 1-butanol at 40 °C from 4 h to 7 days. The changes in starch granule, crystalline, and molecular weight during the 1-butanol–HCl hydrolysis were determined using SEM, particle size analyzer, XRD, HPSEC–MALLS–RI, and iodine staining. SEM and particle size analyzer analysis showed that the granule morphology significantly changed and the granule size decreased from 5.47 μm to 5.07 μm after the hydrolysis by 1-butanol–HCl for 7 days. XRD results indicated that amorphous areas of normal rice starch were firstly hydrolyzed followed by the ordered areas during 1-butanol–HCl hydrolysis. The peak (2 θ ) of V-type crystallinity was detected at around 20° in the XRD curves of 1-butanol–HCl-hydrolyzed starches. This peak confirmed the formation of amylose–1-butanol complex. HPSEC–MALLS–RI and iodine staining analysis demonstrated that the weight-average molecular weight of normal rice starch sharply decreased from 2.4 × 10 7 Da to 4.9 × 10 5 Da after 1-butanol–HCl hydrolysis for 4 h, then gradually decreased, and finally stabilized at about 2.6 × 10 4 Da after hydrolysis for 4 days. These results suggest that the attack of 1-butanol–HCl on normal rice starch preferentially occurred in the amylopectin molecules of amorphous areas.
研究领域:
食品科学
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生物化学
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产品关联
DOI
高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2023
·
IF 2.6
Ao Dong,Taotao Zhan,Yuqi Su,Hongyu He,Weijie Jia,Maogang He,Ying Zhang
The speeds of sound in 1-butanol + ethyl laurate binary mixtures determine the injection time and combustion characteristics of diesel engine. The speed of sound of 1-butanol + ethyl laurate binary mixture system was measured by the Rayleigh–Brillouin light scattering method at temperatures from 293 to 520 K and pressures from 0.1 to 7.5 MPa. A set of parameters was fitted using 1-butanol and ethyl laurate experiment data for the volume-translated Peng–Robinson (VTPR-BE) equation of state (EoS), taking into account the temperature influence on the binary interaction parameter kij. Increasing the order of the temperature function of kij can reduce the calculation deviation of the VTPR-BE EoS. However, beyond the second-order term, further increases in order do not yield significant improvements in accuracy. By adopting the second-order kij form, the density and the speed of sound of 1-butanol + ethyl laurate binary mixture system were calculated using the VTPR-BE EoS. The average absolute relative deviation is 1.05% for density and 3.90% for the speed of sound. Especially, when the molar fraction of 1-butanol is 0.5, the absolute relative deviation of the speed of sound is 1.79%.
研究领域:
热力学
·
物理化学
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产品关联
DOI
高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2017
·
IF 2.323
Tiantian Ye,Haibin Qu,Xingchu Gong
The liquid–liquid equilibrium (LLE) data of ternary systems of water + d-fructose + 1-butanol, water + d-glucose + 1-butanol, and water + d-galactose + 1-butanol were determined at three temperatures (288.2, 303.2, and 318.2 K) and atmospheric condition. The distribution coefficients of the monosaccharides were lower than 0.05. For all the three saccharides, their content in the organic-rich phase increased as their content in the aqueous phase increased. The experimental tie-line data were fitted using the Othmer–Tobias equation. The saccharide universal quasichemical functional-group activity coefficient (S-UNIFAC) model was used to predict the LLE data with all the average absolute deviations (AAD) values higher than 0.013. The modified universal quasichemical model was then used to correlate the LLE data. The correlation results agreed better with experimental data than the prediction results obtained with S-UNIFAC. In the production of traditional Chinese medicines, 1-butanol is a promising extractant used to separate active compounds and saccharides in aqueous herbal extracts.
研究领域:
化学工程
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生物化学
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产品关联
DOI
高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
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2011
·
IF 2.089
Baimei Yang,Hui Wan,Guofeng Guan
Isobaric binary vapor–liquid equilibrium (VLE) data for 1-butanol + ethylbenzene, or o-, or m-, or p-xylene and isobaric quinary VLE data for 1-butanol + ethylbenzene + o-xylene + m-xylene + p-xylene are measured at 101.33 kPa using a modified Rose cell with circulation of both phases. The four binary systems of 1-butanol + ethylbenzene, or o-, or m-, or p-xylene exhibit minimum boiling azeotropes, and the composition of the azeotropes are reported. The VLE data for the four binary systems are checked to meet rigorous thermodynamic consistency by Herington method and the point-to-point test of the Fredenslund method. The nonideality in vapor phase of the measured binary systems is analyzed through calculating fugacity coefficients. Combined with the Hayden–O'Connell (HOC) equation, the VLE data for 1-butanol + ethylbenzene, or o-, or m-, or p-xylene are well-correlated by the nonrandom two-liquid (NRTL), universal quasichemical activity coefficient (UNIQUAC), and Wilson equations. The NRTL model parameters obtained from correlation are used to predict the VLE data of the quinary system. According to the average absolute deviation values, the obtained quinary predicted values are in good agreement with the experimental values.
研究领域:
化学工程
·
热力学
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产品关联
DOI
高影响因子
JOURNAL OF BIOTECHNOLOGY
·
2019
·
IF 3.163
Xiao-Ling Tang,Nan-Nan Zhang,Guo-Yan Ye,Yu-Guo Zheng
( R )-3-amino-1-butanol is a key intermediate of Dolutegravir for the treatment of HIV/AIDS and its green and efficient biosynthesis has attracted a great deal of attention. Transaminases are currently used as promising biocatalyst for the synthesis of chiral amines. However, many transaminases have ( S )-specificity and ( R )-selective transaminases were less exploited and studied, making the production of ( R )-amines remain challenging. In this study, a novel transaminase from Actinobacteria sp. ( As -TA) was obtained and applied for the biosynthesis of ( R )-3-amino-1-butanol by transferring the amino group from isopropylamine to 4-hydroxy-2-butanone. After optimization of the reaction condition and using a substrate fed-batch strategy, the conversion of 100, 200, 300, 400 and 500 mM 4-hydroxy-2-butanone reached 100%, 94.9%, 86.1%, 76.1% and 70.9%, respectively. ( R )-3-amino-1-butanol with a maximum yield of 29.6 g/L and 99.9% e.e. value was obtained. This was the first time demonstrating the successful biosynthesis of ( R )-3-amino-1-butanol with transaminase as biocatalyst and the obtained As -TA enriched the enzyme pool of transaminase with ( R )-specificity.
研究领域:
生物化学
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酶工程
·
药物合成
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DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
·
IF 8.6
Siyu Pang,Zhihao Si,Yan Zhuang,Hanzhu Wu,Jan Baeyens,Xinmiao Zhang,Peiyong Qin
Polydimethylsiloxane (PDMS) is the benchmark pervaporation (PV) material, and its hollow fiber (HF) membrane is generally fabricated by thermal curing, which is energy-intensive and hard to be controlled. For the first time, a continuous and controllable photo-curing system for the fabrication of hydrophobic PDMS HF membrane is proposed. In this process, the uncoated PVDF HF membrane firstly exits from the feed roll, and enters into the tank filled with photo-cured PDMS solution. After being coated by PDMS, the new liquid layer uninterruptedly enters into the UV irradiation module and realizes the photo-curing reaction. Finally, the cured PDMS HF membrane moves into the take-up roll for collection. Due to this technology firstly being assessed, a series of operation parameters are optimized, including 126, 507 g mol −1 of the PDMS molecular weight, 12 wt% of PDMS concentration in its coating solution, 20.1 mW cm −2 of the irradiation intensity, 0.86 m min −1 of the rotation speed of roll and 1 time of the number of dip-coating. Under these parameters, the prepared PDMS HF membrane achieves an outstanding separation factor of 18.2 with a total flux of 1821 g m −2 h −1 for PV separation of 1-butanol. Our study has proven the feasibility of photo-cured fabrication of PDMS HF membranes for 1-butanol pervaporation.
研究领域:
材料科学
·
化学工程
·
分离技术
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DOI
高影响因子
JOURNAL OF CHEMICAL THERMODYNAMICS
·
2020
·
IF 2.888
Xingchuan Yang,Xiangmeng Chen,Chunmei Cao,Li Xu,Guoji Liu
Solubility and liquid–liquid equilibria data for ternary systems for (water + 1,6-hexanediol + 1-butanol/2-methyl-1-propanol) were obtained at (293.15–323.15) K under atmospheric pressure. The LLE data were determined using a double-jacketed glass vessel, and the solubility curves were acquired by the cloud-point method. The distribution coefficient and separation factors were calculated and the reliability of experimental data was assessed by the Othmer–Tobias equation and Hand equation. The measured LLE data were fitted by NRTL and UNIQUAC models and binary parameters were obtained by using of Aspen Plus software. The calculated root-mean-square deviation values show that the calculated results from models showed good agreement with experimental data.
研究领域:
化学工程
·
热力学
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产品关联
DOI
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2020
·
IF 7.183
Zhihao Si,Chang Liu,Guozhen Li,Ze Wang,Jingfang Li,Tanlong Xue,Shuai Yang,Di Cai,Shufeng Li,Haoning Zhao,Peiyong Qin,Tianwei Tan
The ultrashort and controllable membrane-forming process is highly required in large-scale production of PDMS membrane. To shorten the cross-linking time, an UV-induced ring-opening polymerization of the epoxide groups was introduced to the preparation of PDMS membranes. PDMS was firstly functionalized with epoxide groups, providing the possibility of being cross-linked under UV irradiation. With this strategy, the membrane curing was finished within 200 s, hence shortened by two orders of magnitude in comparison with the conventional thermal-driven method. Meanwhile, the cationic photoinitiation of epoxide groups can be achieved without oxygen inhibition in an open-air environment. Moreover, the viscosity of the coating solution in the groove is kept constant when protected from light, thus meeting the requirement of the coatability. When applied to the pervaporation of a 1.5 wt% 1-butanol/water solution at 55 °C, the measured 1-butanol separation factor was 45 and the total flux was 1119 g m −2 h −1 . This novel strategy shows a great prospect in the continuous preparation of membrane.
研究领域:
材料科学
·
化学工程
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产品关联
DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2016
·
IF 2.74
Xue Wang,Mingjie Wang,Zhiyun Chen,Xiaoyi Tao,Weiguo Shen
The liquid-liquid equilibrium data and the specific heat capacities for the binary room temperature ionic solution {1-butanol + 1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide} have been reported. The corresponding critical exponents β and α were obtained and found to be well coincided with the 3D-Ising criticality. The critical complete scaling theory was applied to successfully describe the asymmetric behavior of the diameter of the coexistence curve with the consideration of the heat capacity related term. The large values of the reduced critical density, the reduced critical temperature and the critical amplitude related to the heat capacity were deduced, which indicated a solvophobic criticality of the studied system.
研究领域:
物理化学
·
材料科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）