POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2018
·
IF 3.112
Yurong Ma,Qingfeng Ban,Jingying Shi,Tiantian Dong,Cai-Zhong Jiang,Qingguo Wang
The effects of 1-methylcyclopropene (1-MCP) treatment, storage time, and shelf life and temperature on phenolic concentrations and antioxidant activity in apple peel and flesh of ‘Jonagold’ apple ( Malus domestica Borkh) have been investigated. Hyperoside and chlorogenic acid were the most abundant phenolic compounds in the peel and flesh, respectively. The flesh had lower concentrations of individual phenolics, total phenolics, flavonoids and lower antioxidant activity (determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay) than the peel. In addition, the profiles of phenolics as well as the changing patterns of individual phenolics also differed between the peel and the flesh. After long-time storage (180 d), the greatest decrease of concentrations was the concentrations of hyperoside (27 %) in the peel and total phenolics (35 %) in the flesh. Also, during the 90–180 d storage period, hyperoside and rutin in the peel, chlorogenic acid in the flesh, and flavonoids and antioxidant activity decreased in both peel and flesh tissues. Individual phenolics exhibited different stabilities during the early stages after harvest. 1-MCP reduced the reduction in the concentrations of hyperoside in the peel, chlorogenic acid in the flesh, and total phenolics, flavonoids and antioxidant activity in both the peel and flesh tissues during storage and shelf life. However, for quercetin and rutin, the protective effect of 1-MCP was only observed during the shelf life period. Overall, phenolic compounds responded variously to storage, shelf life, shelf temperature and 1-MCP treatment.
研究领域:
食品科学
·
植物生理学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2026
·
IF 10.4
Maratab Ali,Huizhi Chen,Weijie Wu,Ben Niu,Xiangjun Fang,Chaoyi Shen,Guannan Wang,Hangjun Chen,Ting Huang,Ming Yin,Ruiling Liu,Haiyan Gao
1-MCP upregulated the sugar and organic acid metabolism in goji berry. • Malic and fructose were main organic acid and sugar in goji berry. • 1-MCP interlinked sugar-acid homeostasis with extended shelf life of goji berry. • Molecular docking showed possible 1-MCP led modulations in enzyme function. • 1-MCP maintained postharvest quality and storage potential of goji berries. This study investigated postharvest quality and shelf life of “Ningnongqi 17” goji berries under 1-MCP-mediated sugar-acid metabolism. The results showed that 1-MCP-treated goji berries exhibited delayed firmness loss, TA loss, and color, along with a slower increase in TSS/TA ratio, weight loss, decay rate, and EL than control (CK). 1-MCP enhanced citric, succinic, fumaric, and malic acids accumulation by upregulating PEPC, CS, and NAD-MDH and suppressing ACL, ACO, and SDH enzymes and their transcription. Meanwhile, sucrose levels increased, and glucose and fructose remained lower via downregulated AI, NI, SS-c, and upregulated SPS and SPP enzymes and their transcription. Notably, malic acid and fructose were identified as the main organic acid and sugar in berries. Correlation analysis linked elevated malic, succinic, and sucrose levels with improved firmness, color, TSS, and lower weight loss, decay rate, and EL in treated berries. In conclusion, 1-MCP delayed ripening and senescence by maintaining optimal sugar-acid homeostasis, resulted in improved quality and extended shelf life of fresh goji berries. Download: Download high-res image (250KB) Download: Download full-size image
✓ 已验证
产品关联
DOI
高影响因子
Horticulturae
·
2025
·Vol. 11
·期 11
·1280
·
IF 3
Renchi Chen,Yuhua Yan,Weiliang Guan,Zhihai Huang
Both abscisic acid (ABA) and ethylene play essential roles in the ripening process of strawberry fruit (Fragaria×ananassaDuch.). In this study, the crosstalk between ABA and ethylene involved in strawberry fruit ripening was investigated. The results showed that ethylene triggered a 1.30-fold increase in ABA levels by suppressing the expression of miR396. Meanwhile, the silencing of miR396 (STTM396 reporter) demonstrated that miR396 acts as a negative regulatory factor of ABA biosynthesis by repressingFaNCED2expression. Additionally, dual-luciferase assays indicated a 0.56-fold suppression ofFaNCED2promoter activity by miR396. These findings reveal a novel regulatory mechanism in which ethylene promotes ABA biosynthesis through the suppression of miR396 expression in postharvest strawberry fruit.
✓ 已验证
产品关联
DOI
高影响因子
POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2023
·
IF 7
Renchi Chen,Yingyin Wu,Xiaobo Wei,Zhihai Huang,Linchun Mao
Both ethylene and abscisic acid (ABA) are identified to be crucial phytohormones that positively regulate strawberry fruit ( Fragaria × ananassa Duch.) ripening. In this work, the interplay between ethylene and ABA involved in the ripening of strawberry fruit was explored. Results suggested that ethylene promoted ABA biosynthesis via repressing miR161 expression. Meanwhile, miR161 silencing (STTM161) verified that miR161 negatively regulated ABA biosynthesis via suppressing FaNCED1 expression. In addition, dual-luciferase reporter demonstrated the suppression of miR161 on the promoter of FaNCED1 . These results illuminated a potential molecular mechanism of the collaboration between ethylene and ABA in postharvest strawberry fruit through miR161.
✓ 已验证
产品关联
DOI
高影响因子
Horticulturae
·
2021
·Vol. 7
·期 12
·519
·
IF 2.331
Junwei Wang,Shuxiang Mao,Yiming Yuan,Na Zhang,Qi Wu,Mantian Liang,Shengze Wang,Ke Huang,Qiuyun Wu
The effects of storage conditions and cooking methods on chlorophyll, glucosinolate (GSL), and sulforaphane content in broccoli florets were investigated in this study. For the storage experiment, fresh broccoli florets were stored for 2, 4, and 6 days at 10, 4, and 0 °C with or without 1-methylcyclopropene (1-MCP) treatment. For the cooking experiment, fresh broccoli florets were cooked for 1, 3, 5, 7, and 9 min under three cooking methods, namely, steaming, microwaving, and boiling. Results showed that the contents of chlorophyll, aliphatic GSL, indole GSL, and sulforaphane in broccoli florets of two cultivars decreased with prolonged storage time. The retained contents of chlorophyll, GSLs, and sulforaphane under 0 °C storage condition were significantly higher than those under 10 °C storage condition after 6 days of storage. The sulforaphane content was increased by 1-MCP treatment but differed among varieties. The cooking experiment showed that aliphatic GSL content decreased with increased cooking time under three cooking methods, and indole GSL and sulforaphane contents had a fluctuating trend with increasing cooking time after steaming and microwaving. Sulforaphane content increased by 17.15–50.16% relative to that in fresh broccoli florets and was considerably affected by cooking time. The highest level of sulforaphane content was retained for 7 min during steaming or 5 min during microwaving. Therefore, the combination of 1-MCP treatment and 0 °C storage condition had the best performance in preserving chlorophyll, GSLs, and sulforaphane. Moreover, steaming for 7 min or microwaving for 5 min is a more effective method for preserving the quality and increasing the sulforaphane content of broccoli florets than boiling.Keywords:broccoli;storage;cooking;glucosinolate;sulforaphane
✓ 已验证
产品关联
DOI
高影响因子
Horticultural Plant Journal
·
2025
·
IF 6.2
Yukuo Li,Zhe Song,Xu Zhan,Miaomiao Lin,Xiaohan Li,Ran Wang,Leiming Sun,Hong Gu,Feng Wei,Jinbao Fang,Xiujuan Qi
In kiwifruit ( Actinidia arguta ), fruit coloration is typically accompanied by ripening; however, the intrinsic connection between these two processes remains unclear. In this study, we found that ethylene and auxin accelerated and suppressed fruit coloration and ripening, respectively, in A. arguta . ETHYLENE INSENSITIVE 3-LIKE 2 (AaEIL2) and ETHYLENE RESPONSIVE FACTOR (AaERF059) were mined and identified using yeast two-hybrid library screening and transcriptome analysis. AaEIL2, specifically induced to high expression by ethylene, was confirmed as a transcription factor that positively regulates coloration and ripening by targeting AaLDOX (leucoanthocyanidin dioxygenase involved in anthocyanin biosynthesis) and AaPG18 (polygalacturonase involved in cell wall degradation) based on its subcellular localization in Arabidopsis protoplasts, stable genetic transformation in transgenic tomato, and yeast one-hybrid and luciferase activity assays. AaERF059 also responds to ethylene and regulates ethylene-/auxin-mediated fruit coloration and ripening by targeting the downstream genes AaACS2 (ACC synthase, which is involved in ethylene biosynthesis) and AaGH3 (Gretchen-Hagen 3, which is involved in the auxin pathway). Overall, AaEIL2 and AaERF059 regulate ethylene-and auxin-mediated fruit coloration and ripening by maintaining a dynamic balance in a positive and negative regulatory manner. Our results not only identified key genes but also established an intrinsic connection between fruit coloration and ripening in A. arguta .
✓ 已验证
产品关联
DOI
高影响因子
Science Advances
·
2025
·Vol. 11
·期 33
·
IF 12.5
Wenjing Liang,Sha Huang,Xinran Chen,Kai Jiang,Xin Gong,Hongwei Guo,Lin Li
Shade avoidance syndrome (SAS) commonly occurs in plants sensing shading and the proximity of neighbors, leading to morphological and physiological changes that are detrimental to plant health and consequently crop yield. Controlling SAS could be beneficial for sustainable agriculture. Here, we performed a phenotype-based chemical screening in Arabidopsis and identified pifithrin-α (PFT-α) as a compound that suppresses shade-induced hypocotyl elongation and gene expression. Through genetic, biochemical, and molecular approaches, we demonstrated that PFT-α mimics ethylene by deactivating the ethylene receptor 1 (ETR1). Accumulated ethylene-insensitive 3 (EIN3) influences the expression of phytochrome-interacting factors ( PIF4 and PIF7 ) and subsequently inhibits shade-induced hypocotyl elongation. Our findings not only provide molecular insights into SAS but also suggest that PFT-α, acting as a potent ethylene mimic, plays a crucial role in hormone-regulated plant development and holds potential for modulating SAS in agricultural practices.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Integrative Plant Biology
·
2025
·
IF 9.3
Jiawen Yan,Zhihui Liu,Tongtong Wang,Ruoman Wang,Shuya Wang,Xiao Liu,Ya Liu,Jingwei Yan,Jiasheng Wu
Waterlogging stress is a major abiotic stress that severely limits plant growth and development. However, little is known about the effects of waterlogging stress on the growth and development of gymnosperms. In this study, we demonstrated that the TgRAV1–TgWRKY74–TgACS13 module regulates ethylene-enhanced root vitality during waterlogging stress in the gymnosperm Torreya grandis . Root vitality, the physiological status of root tissues, reflects their metabolic activity and cell viability of roots. Waterlogging stress induces ethylene accumulation in T. grandis roots, thereby enhancing root vitality. The ethylene biosynthesis gene TgACS13 positively regulates root vitality under waterlogging stress by increasing ethylene levels. The transcription factors TgWRKY74 and TgRAV1 directly regulate TgACS13 expression by binding to its promoter. Furthermore, waterlogging stress activates TgWRKY74 to promote TgACS13 transcription and alleviates the inhibitory effect of TgRAV1 on its expression, resulting in ethylene-enhanced root vitality during waterlogging stress. In addition, TgRAV1 interacts with TgWRKY74 both in vivo and in vitro , reducing the transcriptional activity of TgWRKY74 by inhibiting its DNA-binding ability without affecting the transcriptional activity of TgRAV1. Therefore, the TgRAV1–TgWRKY74 module finely tunes TgACS13 expression to regulate ethylene accumulation through multiple mechanisms, thereby maintaining the vitality of T. grandis roots exposed to waterlogging stress.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
·
2025
·Vol. 122
·期 6
·e2417735122
·
IF 9.1
Wangshu Mou,Ria Khare,Joanna K. Polko,Isaiah Taylor,Juan Xu,Dawei Xue,Philip Benfey,Bram Van de Poel,Caren Chang,Joseph J. Kieber
In seed plants, the canonical role of 1-aminocyclopropane-1-carboxylic acid (ACC) is to serve as the precursor in the biosynthesis of the phytohormone ethylene, and indeed, ACC treatment is often used as a proxy for ethylene treatment. Increasing evidence suggests that ACC can also act independently of ethylene to regulate various aspects of plant growth and development. Here, we explore the effects of ACC on Arabidopsis thaliana root growth and the mechanisms by which it acts. ACC inhibits growth of the primary root in Arabidopsis seedlings when ethylene signaling is blocked, which becomes evident after 36 h of treatment with ACC. This reduced root growth is in part the result of suppressed cell proliferation in the root meristem resulting from altered expression of a key regulator of stem cell niche activity, WOX5. ACC also promotes lateral root (LR) development, in contrast to ethylene, which inhibits LR formation. Transcriptomic analysis of roots revealed no significant changes in gene expression after 45 min or 4 h of ACC treatment, but longer treatment times revealed a large number of differentially expressed genes, including the downregulation of the expression of a small group of phylogenetically related CLE peptides. Reduced expression of these group 1 CLEs in response to ACC leads to the activation of a transcription factor, LBD18, which promotes LR development. These results suggest that ACC acts to modulate multiple aspects of Arabidopsis root growth independently of ethylene via distinct transcriptional effects in the root meristem and LR precursor cells.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Xiaoqin Wu,Zhiyi Shan,Lufan Wang,Wenwei Shi,Yanyan Li,Hongfang Cai,Liang Zhou,Chen Huan
Anthocyanins are bioactive substances that are not only responsible for the pigmentation of various fruits, but also hold potential benefits to human health. In this study, postharvest peaches were treated with 10 ppm 1-MCP, and changes of anthocyanin biosynthesis were then revealed at transcriptional level to gain insight into the underlying molecular mechanisms. The functions of key transcription factors (TFs) were systematically analyzed. Results demonstrated that 1-MCP effectively up-regulated expression of structural genes and stimulated anthocyanin accumulation. A novel transcription factor PpbHLH135 can respond positively to 1-MCP treatment and has been noted for its remarkable gene expression and protein abundance. The transcription factor bHLH3 was also investigated in this study due to its widespread interest and attention, serving to compare the regulatory function of bHLH135 in anthocyanin synthesis, although it didn’t show a significant response to 1-MCP treatment in this study. PpbHLH3 TF showed a stronger regulatory effect on PpF3H , while PpbHLH135 was more dominant over the regulation of PpUFGT . Future genetic and molecular experimentation will elucidate whether PpbHLH135 TF, in combination with other TFs, can play a stronger regulating role in fruit pigmentation, or in other metabolic pathways.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）