JOURNAL OF HAZARDOUS MATERIALS
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2024
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IF 12.2
Se-Ruo Li,Ning-Ning Kang,Rong-Rong Wang,Meng-Die Li,Li-Hong Chen,Peng Zhou,De-Xiang Xu,Hui Zhao,Lin Fu
Our previous study revealed that 1-nitropyrene (1-NP) exposure evoked pulmonary fibrosis in mice. However, the exact mechanism remained elusive. We found that 1-NP induced telomere damage and cellular senescence in mice lungs, and two alveolar epithelial cells lines. 1-NP downregulated telomere repeat binding factor 2 (TRF2), and upregulated FBXW7. Mechanistically, 1-NP-caused TRF2 ubiquitination and proteasomal degradation depended on E3 ubiquitin ligase activity of FBXW7. Moreover, 1-NP upregulated FBXW7 m6A modification via an ALKBH5-YTHDF1-dependent manner. Further analysis suggested 1-NP promoted ALKBH5 SUMOylation and subsequent proteasomal degradation. Additionally, 1-NP evoked mitochondrial reactive oxygen species (mtROS) overproduction. Mito-TEMPO, a mitochondrial-targeted antioxidant, mitigated 1-NP-caused mtROS overproduction, ALKBH5 SUMOylation, FBXW7 m6A modification, TRF2 degradation, cellular senescence, and pulmonary fibrosis. Taken together, mtROS-initiated ALKBH5 SUMOylation and subsequent FBXW7 m6A modification is indispensable for TRF2 degradation and cellular senescence in alveolar epithelial cells during 1-NP-induced pulmonary fibrosis. Our study provides target intervention measures towards 1-NP-evoked pulmonary fibrosis. Environmental Implication 1-nitropyrene (1-NP) is a representative of nitro polycyclic aromatic hydrocarbon compound that can be formed during the insufficient combustion of organic substances like coal, oil, gas, and tobacco. Moreover, mounting researches have revealed that 1-NP can cause various respiratory system diseases, inclusive of acute lung injury, chronic obstructive pulmonary disease, and allergic asthma. The current research provides evidence that exposure to 1-NP induces pulmonary fibrosis through evoking telomere damage and cellular senescenc. What’s more, Mito-TEMPO, a mitochondrial-targeted antioxidant, can mitigate 1-NP-caused cellular senescence and pulmonary fibrosis. Our study provides target intervention measures towards 1-NP-evoked pulmonary fibrosis.
研究领域:
毒理学
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肺科学
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细胞生物学
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分子生物学
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环境健康
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PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2026
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IF 6.6
Hanming Yu,Miao Li,Xin Kang,Yi Dai,Fei Li,Yuan Zhang,Qiuhong Li
Background Nitro-aromatic compounds (NACs) are pervasive environmental pollutants with recognized pulmonary toxicity, yet their mechanistic involvement in pulmonary fibrosis remains incompletely understood. Methods We applied an integrative framework combining network toxicology, transcriptomic analysis, prognostic modeling, immune infiltration profiling, molecular docking, and molecular dynamics (MD) simulations to investigate the fibrogenic effects of two representative NACs, 1-nitropyrene (1-NP) and nitrobenzene (NB). Identification and evaluation of NACs-related targets and their clinical relevance using the idiopathic pulmonary fibrosis (IPF) GEO dataset. Results Network analysis using eight topological algorithms identified ten key genes occupying central positions in the NACs-associated fibrosis-related candidate network. Among these, BCL2L1 and MYC exhibit significant prognostic significance in IPF.Risk models based on hub genes effectively stratify patient survival rates and are closely associated with immune dysregulation, characterized by macrophage aggregation and CD8⁺ T cell exhaustion. Molecular docking analysis indicates that 1-NP and NB exhibit strong binding affinity with the anti-apoptotic protein BCL2L1 . Notably, 100-ns MD simulations revealed that NACs binding was associated with significant conformational destabilization and partial unfolding of BCL2L1 , as evidenced by sustained increases in Root mean square deviation (RMSD), radius of gyration (Rg), and solvent-accessible surface area (SASA). These findings support a non-classical mechanism of structural toxicity, wherein NACs exert their effects by disrupting protein stability rather than acting as classical enzyme inhibitors. Conclusions This study suggests a potential mechanism by which environmental NACs may contribute to fibrotic progression by disrupting the structural stability of BCL2L1 and reshaping the immune microenvironment. Our findings elucidate a potential molecular association between environmental exposure and fibrosis progression, suggesting that BCL2L1 -mediated structural toxicity may serve as a potential biomarker and therapeutic target for pollution-related pulmonary fibrosis.
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高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
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2025
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IF 9
Zhuo Chen,Zhiyang Lu,Weijun Tian,Bingkun Liu,Bingjie Huo,Jing Zhao,Mengyuan Zou,Ruijuan Zhang,Xinran Yu,Xinbo Wang
Pyrene (Pyr) and its derivatives are classified as Persistent Organic Pollutants (POPs), which exhibit significant toxicological effects and pose serious threats to ecosystems. Photocatalytic technology is increasingly employed for the remediation of POPs due to its effective and environmentally sustainable degradation capabilities. However, conventional photocatalytic materials, primarily in powder form, face challenges related to separation and recovery from aqueous environments, significantly limiting their practical application. In this study, we successfully synthesized a three-dimensional (3D) porous immobilized photocatalytic material by decorating Ag 3 PO 4 and BiOBr onto Ni foam (NF) using a hydrothermal method combined with continuous ionic layer adsorption. The results demonstrated Ag 3 PO 4 /BiOBr/NF exceptional light degradation capabilities, achieving over 90 % degradation of Pyr, 1-methylpyrene, and 1-hydroxypyrene, as well as over 40 % degradation of 1-nitropyrene under visible light irradiation over a duration of 5 h. Its degradation efficiency decreased by 12.49 % after five cycles, demonstrating its reusability. The roles of superoxide radicals ( O 2 – ) and holes (h + ) were critical in the degradation process. Furthermore, based on experimental findings, we proposed a Z-type heterojunction degradation mechanism for the Ag 3 PO 4 /BiOBr/NF composite, accompanied by an analysis of the potential degradation pathways for Pyr. Consequently, the recyclable immobilized photocatalytic materials developed in this research contribute novel insights into the photocatalytic degradation of POPs.
研究领域:
环境科学
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材料科学
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DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
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2024
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IF 3.8
Qingwen Tao,Pu Ma,Beining Chen,Xiaolei Qu,Heyun Fu
Nitrated polycyclic aromatic hydrocarbons (nitro-PAHs) are pervasive contaminants in aquatic environments. They are characterized by persistence, toxicity, bioaccumulation, and long-range transport, significantly threatening human health. The development of sensitive methods for nitro-PAH analysis in environmental samples is in great need. This study developed a novel carbonaceous SPME coating derived from metal-organic framework (MOF), namely a spherical assembly consisting of carbon nanorods with hierarchical porosity (HP-MOF-C), for the extraction and determination of nitro-PAHs in waters. The HP-MOF-C coated fiber demonstrated superior nitro-PAH extraction efficiencies, with enrichment factors 2∼70 times higher than commercial fibers. This enhancement was due to the strong hydrophobic, π-π electron coupling/stacking, and π-π electron donor-acceptor interactions between the carbonaceous framework of HP-MOF-C and the nitro-PAHs. Moreover, the unique hierarchical porous structure of HP-MOF-C accelerated the diffusion of nitro-PAHs, further facilitating their enrichment. The fiber also exhibited good thermal stability, remarkable chemical stabilities against common acid, base, and polar/non-polar solvents, and long service life (> 150 SPME cycles). The nitro-PAH determination method based on HP-MOF-C coating yielded wide linear ranges, low detection limits (0.4∼5.0 ng L -1 ), satisfactory repeatability and reproducibility, and good recoveries in real water samples. The proposed method was considered to be green according to the Analytical GREEnness assessment. The present study not only offers an efficient SPME coating for the enrichment of nitro-PAHs, but also provides insights into the design of porous coating materials.
研究领域:
环境科学
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分析化学
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材料科学
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PubMed
高影响因子
ANALYTICAL CHEMISTRY
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2021
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IF 6.986
Yue Lin,Xiu Huang,Yongchun Liu,Dong Cao,Dawei Lu,Zeming Feng,Qian Liu,Zhenyu Lin,Guibin Jiang
Soot is ubiquitous and has large detrimental effects on climate, air quality, and human health. However, identification of soot in carbonaceous media is very challenging due to its nanoscale carbon nature and complex sources. Due to the shortage in the methodology, until now, the fate and health effect of soot particles after inhalation are still poorly understood. Here, we report a new method for label-free identification, quantification, and imaging of soot particles in complex media based on laser desorption/ionization mass spectrometry fingerprinting. We found that soot particles from different origins and with different morphologies showed highly consistent mass spectral fingerprints deriving from peak ratios of small carbon cluster anions (C2––C10–), which enabled both accurate quantification of soot in fine particulate matter (PM2.5) samples and label-free imaging of soot particles in biological media. By using this technique, we tracked and imaged the suborgan distribution of soot particles in mice after exposure to PM2.5. The results showed that the lung is the main target organ for short-term inhalation exposure to soot particles. This study helps to better understand the inhalation toxicology of soot and also provides a practical novel methodological platform for identification, tracing, and toxicological studies of elemental carbon-based nanomaterials.
研究领域:
环境科学
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分析化学
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毒理学
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高影响因子
Nanophotonics
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2022
·Vol. 11
·期 12
·2875-2889
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IF 7.923
Mengya Zhang,Yue Tian,Anxin Jiao,Hui Ma,Chang Wang,Linqi Zheng,Shuang Li,Ming Chen
Ultrasensitive trace-detection of toxic and carcinogenic polycyclic aromatic hydrocarbons (PAHs) can ceaselessly propel the environmental surveillance in aqueous ecosystems. Due to the intrinsic nonadsorptive feature of PAHs, the promising technique of surface-enhanced Raman scattering (SERS) spectroscopy has been restricted to diverse functional ligands-based surface modifications of nano-substrates. However, it is not suitable for practical ultralow liquid analysis. Herein, we propose an extraordinary strategy to boost liquid-near infrared (NIR)-SERS activity of plasmonic Au/Ag nano-urchins (NUs) by introducing extra 808 nm laser-triggered an additional strong electromagnetic enhancement into routine 785 nm laser-Raman system. The synergistic double laser-excited NIR-SERS of colloidal Au/Ag NUs enables the Raman signals of crystal violet to be significantly enhanced, approaching a maximum of ∼34-fold increase than that of traditional bare 785 nm laser-excitation. More importantly, the improved liquid-NIR-SERS enables the in-situ detection limit of pyrene molecules in lake water to be achieved at ∼10 −9 M, which is already better than many previous SERS results based on the complicated functionalized nano-substrates. The established double laser-boosted NIR-SERS can also be easily extended to the simultaneous trace-detection of three PAHs-contaminated mixtures, supporting well distinguishable capability. Undoubtedly, the present work opens a new versatile and innovative avenue for ultrasensitive NIR-SERS monitoring of nonadsorptive toxic pollutants in wastewater.
研究领域:
环境科学
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分析化学
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材料科学
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光学
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高影响因子
ANALYTICAL CHEMISTRY
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2022
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IF 8.008
Ke Min,Yong Li,Yue Lin,Xuezhi Yang,Zigu Chen,Bo Chen,Ming Ma,Qian Liu,Guibin Jiang
Soot, mainly derived from incomplete combustion of fossil fuel and biomass, exists ubiquitously in different environmental matrixes. To study the detrimental effects of soot on climate, air quality, and human health, accurate quantification of soot is an important prerequisite. However, until now, quantification of soot in environmental media, especially in carbonaceous media, is still very challenging. Here, we report a matrix-free laser desorption/ionization mass spectrometry (LDI-MS) method for in situ imaging of soot particles in size-segregated aerosol samples collected on filter membranes. A series of round-shaped sample spots in filter membranes were selected and subjected to MS imaging analysis, enabling direct in situ quantification of soot without solvent extraction or separation. Especially, the MS imaging with serial sample spots can overcome the problems of sweet-spot in LDI-MS and inhomogeneous distribution of soot in the filter membrane, thus greatly improving the precision of quantification. The limit of detection of soot was 4 ng/m2 and the recovery was 84.4–126%. By using this method, we found that a higher soot content was present in larger-sized particulate matter than smaller-sized particles, suggesting that aerosol soot was mainly derived from primary emission sources. Furthermore, this method also shows the potential to analyze nitrate and sulfate species in PM2.5. To the best of our knowledge, it is the first method capable of simultaneous analysis of inorganic salts and soot in air samples. It represents a novel strategy for in situ quantification of aerosol soot with the advantages of high specificity, high sensitivity, separation-, solvent- and matrix-free.
研究领域:
环境科学
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分析化学
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PubMed
高影响因子
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