JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2021
·Vol. 168
·期 5
·057509
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IF 4.316
Yuan Li,Yutong Li,Chao Du,Pengju Zhang,Peipei Cen,Xinsheng Liu,Yonghong Li
Urinary 1-hydroxypyrene (1-OHP) is generally used as an exposure biomarker of polycyclic aromatic hydrocarbons (PAHs). In this work, a colloidal gold modified zeolitic imidazolate frameworks-67/carbon nanofiber paste electrode (Au@ZIF-67/CNFPE) was fabricated for the rapid and sensitive detection of 1-OHP. The morphologies of carbon nanofibers (CNF), ZIF-67, colloidal gold and colloidal gold@ZIF-67 (Au@ZIF-67) were studied by transmission electron microscopy (TEM). The electrochemical behavior of 1-OHP was investigated by square wave voltammetry (SWV). The effect factors like pH value, amount of colloidal gold, accumulation potential and accumulation time have been optimized. Under the optimal conditions, the constructed sensor responded to 1-OHP linearly in the concentration range of 0.0005–10 μM, and the detection limit was 0.1 nM. The reproducibility, stability and selectivity of this sensor were also examined. Finally, the electrode was successfully applied for the analysis of 1-OHP in human urine samples.
研究领域:
分析化学
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环境科学
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生物医学工程
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DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2020
·
IF 3.594
Yuan Li,Yutong Li,Yan Wang,Guodong Ma,Xinsheng Liu,Yonghong Li,Jeffrey Soar
1-hydroxypyrene (1-OHP) is an important biomarker for evaluating human exposure to polycyclic aromatic hydrocarbons (PAHs). In this work, a simple sensor based on nano-carbon paste electrode modified with zeolitic imidazolate frameworks (ZIF-8)/ionic liquid composites was developed for convenient and fast electrochemical detection of 1-OHP. The structure of ZIF-8 and the morphologies of different electrodes were investigated by transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The electrochemical behavior of l-OHP on the constructed modified electrode was studied using differential pulse voltammetry (DPV). Under the optimized conditions, the fabricated sensor gave a linear calibration curve of the concentration ranging from 0.05 to 20 μM with a detection limit of 0.01 μM for 1-OHP. The proposed method was successfully applied to the determination of 1-OHP in urine samples with the recoveries of 94.2%–103.2%.
研究领域:
环境科学
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分析化学
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生物医学
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DOI
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 11
·1-9
·
IF 5.479
Xue Jin-Hua,Liu Ling,Wang Yong-Sheng,Li Jia-Qian,Li Mei,Qu Yue-Ning,Li Le
This work describes a method for the determination of 1-hydroxypyrene (OH-Py) via aggregation-induced quenching of the emission of protamine-coated gold nanoclusters using 9-hydroxyphenanthrene (OH-Phe) as a sensitizer to boost the emission efficiency of nanoprobe. Under optimum conditions, the drop in fluorescence intensity at excitation/emission wavelengths of 300/596 nm is proportional to the concentrations of OH-Py in the range from 1.0 to 65 nM. The relative standard deviations are 4.2, 2.4 and 1.9% (for n = 11) at concentration levels of 8.0, 32 and 48 nM of OH-Py, respectively. The detection limit is 0.3 nM which is much lower than that of some previously reported methods. The recoveries from urine samples spiked with OH-Py ranged between 94.4 and 98.8%.
1-Hydroxypyrene (OH-Py) can trigger the aggregation of protamine-gold nanoclusters (PRT-AuNCs), resulting in the emission quenching of PRT-AuNCs. 9-Hydroxyphenanthrene (OH-Phe) can boost the emission efficiency of nanoprobe. Thereby, a highly sensitive assay of OH-Py was established.
研究领域:
分析化学
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环境医学
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纳米生物学
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产品关联
DOI
PubMed
高影响因子
Journal of Environmental Sciences
·
2025
·
IF 6.3
Jin Chen,Yiwei Fang,Borui Sun,Ying Xu,Wanyi Chen,Kai zhao,Huiping Zhang
Polycyclic aromatic hydrocarbons (PAHs) are widespread pollutants from incomplete combustion of organic materials, linked to various health issues and pregnancy complications. This study explored the relationship between PAH metabolites and gestational hypertension. Urine samples from 118 normal pregnant women and 40 with gestational hypertension were analyzed. In vitro experiments using human trophoblast cells (HTR-8/SVneo) exposed to these metabolites assessed their effects on cellular metabolism. Comprehensive metabolomics analysis was performed using Tidymass and DRomics to integrate global and dose-response metabolomics data. Results showed higher levels of 1-hydroxypyrene (1-OHPYR) and 9-Hydroxyphenanthrene (9-OHPHE) linked to gestational hypertension, inhibiting trophoblast cell invasion and migration. Identified markers include Isobutyryl-L-carnitine, methyl-3-hydroxybutyric acid, threonine, and glutamic acid for 1-OHPYR, and phosphocreatine for 9-OHPHE. This study highlights the connection between PAH metabolites and gestational hypertension, suggesting potential biomarkers and mechanisms. Future research should confirm these findings and explore prevention and treatment strategies.
研究领域:
环境健康
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生殖医学
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代谢组学
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产品关联
DOI
PubMed
高影响因子
ANALYST
·
2015
·Vol. 140
·期 13
·4662-4667
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IF 4.107
Yaqi Hu,Chunyan Du,Yunchao Li,Louzhen Fan,Xiaohong Li
Direct and rapid detection of 1-hydroxypyrene (1-OHP) is of great importance owing to its high carcinogenicity, teratogenicity and toxicity. In this study, a simple colorimetric assay for rapid determination of 1-OHP is reported, which is based on non-crosslinking aggregation of gold nanoparticles (Au NPs) induced by 1-OHP in the presence of formic acid (FA). Initially, Au NPs were synthesized with citrate as the capping agent and exhibited red color. Subsequently, the addition of FA did not cause aggregation of Au NPs, but a proton transfer process occurred from FA to carboxylic anions on the surface of Au NPs with a decreased zeta potential. The subsequent addition of 1-OHP resulted in a further decreased zeta potential and an intensely hydrophobic environment, which led to a strong and rapid non-crosslinking aggregation of Au NPs within 5 min with the color changing from red to violet blue. Based on this principle, sensitive and selective detection of 1-OHP was achieved. The detection limit was 3.3 nM. Finally, the colorimetric assay was successfully applied to detect 1-OHP in a urine sample. This strategy provides new insights into developing colorimetric methods for on-site and real-time detection of polycyclic aromatic hydrocarbons.
研究领域:
环境科学
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分析化学
·
毒理学
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产品关联
DOI
PubMed
高影响因子
ACS Omega
·
2021
·
IF 3.512
Hong Cui,Shuaishuai Cui,Qiuju Tian,Siyuan Zhang,Mingxiu Wang,Ping Zhang,Yunfeng Liu,Jialing Zhang,Xiangjun Li
A nanocomposite was formed by combining graphene oxide (GO) with chromium-centered metal–organic framework (Cr-MOF) nanoparticles regulated by the dendrimer polyamidoamine (PAMAM). PAMAM can successfully regulate the synthesis of Cr-MOF; in doing so, the size of Cr-MOF is reduced, its original morphology is maintained, and it has good crystallinity. A simple ultrasonication method was used to make the Cr-MOF/GO hybrid nanocomposite. Various characterization methods confirmed the successful synthesis of PAMAM/Cr-MOF/GO nanocomposites. The PAMAM/Cr-MOF/ERGO modified electrode could be used with cyclic voltammetry (CV) and differential pulse voltammetry (DPV) to study the electrochemical behaviors of 1-hydroxypyrene (1-OHPyr). The results indicated that the constructed PAMAM/Cr-MOF/ERGO electrochemical sensor had a significantly enhanced electrocatalytic effect on the electrochemical reduction of 1-OHPyr compared with the sensors with no PAMAM and the ERGO sensor, which could be ascribed to the synergetic effect from the high porosity of Cr-MOF and the high conductivity of ERGO, as well as the further electron transport action of the nanocomposite. Under the optimal conditions, the reduction peak current and concentration of 1-OHPyr showed a good linear relationship in the range of 0.1–1.0 and 1.0–6.0 μM, and the detection limit of 1-OHPyr was calculated to be 0.075 μM. Moreover, the PAMAM/Cr-MOF/ERGO electrochemical sensor constructed in this paper can be expected to provide some instructions for the construction of electrochemical sensing platforms and wider potential applications.
研究领域:
分析化学
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环境科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2020
·
IF 4.389
Jin-Hua Xue,Ke-Ping Xiao,Yong-Sheng Wang,Ling Liu,Jia-Qian Li,Mei Li,Yue-Ning Qu,Xi-Lin Xiao
In this study, we confirmed that protamine-templated gold nanoclusters (PRT-AuNCs) exhibit aggregation-induced emission properties (AIE-PRT-AuNCs). 1-Hydroxypyrene (1−OHPy) further induced the aggregation of AIE-PRT-AuNCs via hydrogen bonding and electrostatic and hydrophobic interactions, resulting in the aggregation-induced photoluminescence enhancement of AIE-PRT-AuNCs. 9-Hydroxyphenanthrene was able to decrease the background signal, thus increasing the sensitivity of the method. Based on these findings, a cost-effective, highly sensitive and selective strategy was proposed for the quantitative detection of 1−OHPy. This method displayed a wide linear range of 0.924 – 74.1 nmol/L with a low detection limit of 0.277 nmol/L, showing great potential for the monitoring of 1−OHPy in human urine. This strategy may provide a theoretical basis for future studies of the AIE properties of metal nanoclusters and their applications in the field of chemical and biological sensing.
研究领域:
分析化学
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生物医学工程
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环境科学
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DOI
PubMed
高影响因子
POLYMER
·
2019
·
IF 3.771
Yuehong Pang,Nianci Yang,Xiaofang Shen,Yi Zhang,Liheng Feng
Urinary 1-hydroxypyrene (1-OHP), one of the metabolite of polycyclic aromatic hydrocarbons (PAHs), has been extensively applied as a biomarker of animals and human exposure to PAHs. Herein, we synthesized a novel conjugated polymer poly-8-methoxy-7-methyl-5-(10-anthracen-9-yl)quinolone (PMMAYQ) using Suzuki coupling reaction, and then fabricated a homogeneous PMMAYQ/grahene multilayer film via layer-by-layer self-assembly technique and applied for electrochemical sensing of 1-OHP. Under the optimal conditions, {PMMAYQ-Graphene} 16 modified glass carbon electrode exhibited excellent response for detection of 1-OHP. A linear range from 0.5 to 120 nM was obtained with a low detection limit of 0.07 nM (S/N = 3) and a sensitivity of 0.5539 μA/μM. The electrochemical sensor exhibited excellent stability, accepted repeatability, and good selectivity. The developed method was employed for determination of 1-OHP in human urine samples and recoveries in the range of 95.6%–107.1% were achieved.
研究领域:
分析化学
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环境科学
·
医学
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产品关联
DOI
高影响因子
ELECTROCHIMICA ACTA
·
2011
·
IF 3.65
Xiaofang Shen,Yan Cui,Yuehong Pang,He Qian
1-Hydroxypyrene (1-OHP) is a ubiquitous metabolite of polycyclic aromatic hydrocarbons (PAHs) and has been widely used as a biomarker for evaluation of human exposure to PAHs. In this paper, an electrochemical sensor consisted of graphene oxide nanoribbon (GON)/octa(3-aminopropyl)octasilae quioxane octahydrochloride (OA-POSS) framework films (GON/OA-POSS) modified electrode for pre-concentrating and sensing of 1-OHP was described. The GON/OA-POSS composite films were prepared by hierarchically layer-by-layer assembly via electrostatic interactions. GON, which has higher electrical conductivity than that of graphene oxide, offers residual π-conjugated systems for adsorption of 1-OHP via π–π stacking interaction. Meanwhile, OA-POSS nanocages make the GON layers frameworked to enhance contacting between GON and 1-OHP. When 1-OHP was pre-concentrated on GON/OA-POSS frameworks modified electrode, the response signal and adsorption stability was enhanced significantly. Differential pulse voltammetry was used for direct determination of 1-OHP. The electrochemical sensor calibration curve had a range of 0.1–12.55 μM ( R = 0.995) with a detection limit of 0.04 μM. The results showed that the GON/OA-POSS framework films modified electrode established a new way for simple and sensitive analysis of 1-OHP.
研究领域:
环境科学
·
分析化学
·
材料科学
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DOI
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 5
·1-9
·
IF 5.479
Chen Dan,Xu Hui
The exploration of monohydroxy polycyclic aromatic hydrocarbons and 8-hydroxy-2′-deoxyguanosine (8-OHdG) produced by oxidative stress and DNA damage is a powerful and non-invasive tool to study the health risk of exposure to polycyclic aromatic hydrocarbons (PAHs). A nanocomposite prepared from graphene oxide, poly(3,4-ethylenedioxythiophene) and polypyrrole was electrodeposited on the internal surface of a stainless-steel tube for online in-tube solid phase microextraction (IT-SPME) of 8-OHdG, 3-hydroxyphenanthrene and 1-hydroxypyrene from urine. The coating possesses excellent chemical and mechanical stability, high extraction efficiency, good resistance to matrix interference, and a long lifespan. An online IT-SPME-high performance liquid chromatography-mass spectrometry method was developed for the determination of these three metabolite biomarkers in human urine. Figures of merit include (a) enrichment factors of 30–48; (b) low limits of detection (4–41 pg·mL −1 at S/ N = 3); (c) wide linear ranges (0.05–50 ng·mL −1 ); (d) good recoveries from spiked samples (71.6–109.5%); and (e) acceptable repeatability (2.3–14.6%). The method offers the advantages of low cost, simplicity, sensitivity, rapidity and automation.
Schematic illustration of online in-tube solid phase microextraction using graphene oxide/poly(3,4-ethylenedioxythiophene)/polypyrrole composites as adsorbent in a stainless-steel (SS) tube for the enrichment and simultaneous determination of 8-hydroxy-2′-deoxyguanosine, 3-hydroxyphenanthrene and 1-hydroxypyrene prior to HPLC-MS analysis.
研究领域:
分析化学
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环境健康
·
毒理学
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Yang Yang,Yifan Li,Qinhui Xie,Bo Jiang,Junbo Li,Yunfei Xie,Wei Ji
Biomimetic cytochrome P450 for chemical activation of environmental carcinogens is an efficient in vitro model for evaluating their mutagenicity and ultimately acquiring the metabolites that cannot be easily accessed by conventional routes of organic synthesis. Different kinds of mutagen derived from polyaromatic hydrocarbons (PAHs) by metalloporphyrin/oxidant model systems have been reported, but the underlying molecular mechanisms are poorly understood. Herein, we have for the first time demonstrated an effective surface-enhanced Raman scattering (SERS) protocol to study the dynamics and biomimetic metabolic behaviors of pyrene (Pyr) in the presence of various oxygen donors. Quantitative information on the relative concentration of Pyr and its metabolites in the biomimetic system can be extracted from the SERS spectra. On the basis of our results, we conclude that the oxidative metabolism of Pyr is highly influenced by the types and concentrations of oxygen donors, leading to the formation of 1-hydroxypyrene and dioxygenated products. Besides, the addition of an appropriate amount of an organic solvent can promote the formation of secondary oxidation products. These results offer valuable insights into the dynamics of PAHs metabolism and the regulation of their metabolic pathways in biomimetic activation. In comparison to traditional liquid chromatography–mass spectrometry, the present SERS approach is more suitable for high-throughput evaluation of the metabolic process and kinetics of PAHs. We anticipate that this approach will enable a more general and comprehensive tracking of metabolic dynamics and molecular mechanisms involved in the biomimetic activation of other xenobiotics, such as procarcinogens, promutagens, and drugs.
研究领域:
环境科学
·
分析化学
·
生物化学
·
毒理学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2024
·
IF 3.8
Haidong Gao,Shucai Gao,Ming Lv,Yeru Liu,Jingjing Yao,Hongwei Liu,Fayun Wang,Mantang Chen,Libin Wan
Polycyclic aromatic hydrocarbons (PAHs) have been classified as the earliest discovered class of environmental carcinogens, seriously endangering human health and ecological safety, and the hydroxylpolycyclic aromatic hydrocarbons (OH-PAHs) in human urine are considered as the main biomarkers to evaluate the exposure levels of PAHs in human body. Therefore, it is necessary to develop an accurate and sensitive method for the determination of urinary OH-PAHs. Herein, we proposed electromembrane extraction (EME) as a simple, effective and ecofriendly sample pretreatment technique for selective extraction, purification and enrichment of four typical OH-PAHs (2-naphthol, 2- and 3-phenanthrol, 2-hydroxyfluorene) in human urine for the first time. Under the optimum conditions, an analytical method of EME coupled with HPLC was established, which provided wide linear ranges for four OH-PAHs from 1 to 500 ng mL -1 with low LODs of 0.05–0.3 ng mL -1 . The average recoveries of four OH-PAHs at three spiked levels in human urine were 81.6–102.5% with RSDs all below 9.4%. The present method has been successfully applied for the sensitive determination of trace four OH-PAHs in urine samples from non-smokers and smokers with a maximum concentration of 2.24 and 3.56 ng mL -1 , respectively, which showed a great potential for the analysis of trace OH-PAHs in biological samples.
研究领域:
环境科学
·
分析化学
·
公共卫生
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）