Advanced Healthcare Materials
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2025
·
IF 9.6
Xiaoliang Qi,Yulong Lan,Jing Chen,Yajing Xiang,Yingying Wang,Liting Jiang,Yujie Dong,Jiaxin Li,Zhiyong Liao,Zhangping Li,Jianliang Shen
Current guidelines for addressing multidrug-resistant bacteria-infected diabetic foot ulcers (DFUs), a leading cause of disability and death among diabetes sufferers, still lack specificity. Such DFU lesions often experience delayed recovery, primarily due to the bacteria-induced inflammation in the adverse diabetic microenvironment. Here, an endogenous adenosine triphosphate (ATP)-responsive hydrogel prodrug platform (abbreviated as HSAQ3), which embeds hemoglobin@zeolitic imidazolate framework-8 (Hb@ZIF-8) nanoparticles in a prodrug (1-naphthylacetic acid, NAA)-loaded biopolymer matrix, targeting multidrug-resistant bacterial infections in DFUs is presented. Initially, using a simple local injection, an HSAQ3 adhesive barrier triggered by UV light is applied to the wound. Concurrently, HSAQ3's composition, enriched with quaternary ammonium salt and phenylboronic acid groups, exhibits strong bacterial trapping capabilities, effectively capturing bacteria at the wound location within the hydrogel. Following this, ATP secreted by bacteria initiates the degradation of Hb@ZIF-8, enabling the simultaneous interaction of the encapsulated NAA prodrug with Hb peroxidase. This process effectively produces reactive oxygen species (ROS) in situ, addressing their limited lifespan and diffusion range, thus guaranteeing a highly efficient bactericidal effect. This study unveils an innovative inorganic–organic hybrid prodrug system, leveraging endogenous ATP from bacteria for precise ROS generation, enhancing the healing of multidrug-resistant bacterial infections in DFUs.
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Frontiers in Bioengineering and Biotechnology
·
2023
·
IF 5.7
Ling Li-Juan,Wang Meng,Pan Chuan-Qing,Tang Dao-Bang,Yuan En,Zhang Yuan-Yuan,Chen Ji-Guang,Peng Da-Yong,Yin Zhong-Ping
Background: Plant cell culture technology is a potential way to produce polyphenols, however, this way is still trapped in the dilemma of low content and yield. Elicitation is regarded as one of the most effective ways to improve the output of the secondary metabolites, and therefore has attracted extensive attention.Methods: Five elicitors including 5-aminolevulinic acid (5-ALA), salicylic acid (SA), methyl jasmonate (MeJA), sodium nitroprusside (SNP) and Rhizopus Oryzae Elicitor (ROE) were used to improve the content and yield of polyphenols in the cultured Cyclocarya paliurus (C. paliurus) cells, and a co-induction technology of 5-ALA and SA was developed as a result. Meanwhile, the integrated analysis of transcriptome and metabolome was adopted to interpret the stimulation mechanism of co-induction with 5-ALA and SA.Results: Under the co-induction of 50 μM 5-ALA and SA, the content and yield of total polyphenols of the cultured cells reached 8.0 mg/g and 147.12 mg/L, respectively. The yields of cyanidin-3-O-galactoside, procyanidin B1 and catechin reached 28.83, 4.33 and 2.88 times that of the control group, respectively. It was found that expressions of TFs such as CpERF105, CpMYB10 and CpWRKY28 increased significantly, while CpMYB44 and CpTGA2 decreased. These great changes might further make the expression of CpF3′H (flavonoid 3′-monooxygenase), CpFLS (flavonol synthase), CpLAR (leucoanthocyanidin reductase), CpANS (anthocyanidin synthase) and Cp4CL (4-coumarate coenzyme A ligase) increase while CpANR (anthocyanidin reductase) and CpF3′5′H (flavonoid 3′, 5′-hydroxylase) reduce, ultimately enhancing the polyphenols accumulationConclusion: The co-induction of 5-ALA and SA can significantly promote polyphenol biosynthesis in the cultured C. paliurus cells by regulating the expression of key transcription factors and structural genes associated with polyphenol synthesis, and thus has a promising application.
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CHINESE JOURNAL OF CATALYSIS
·
2023
·
IF 16.5
Zhipeng Huang,Yang Yang,Junju Mu,Genheng Li,Jianyu Han,Puning Ren,Jian Zhang,Nengchao Luo,Ke-Li Han,Feng Wang
Radicals are key intermediates in numerous reactions. Their high reactivity enables various transformations to occur under mild conditions, however, also brings great challenges to control their reactions especially over heterogeneous catalysts. Here we propose to use metal nanoparticles to directly steer the conversion of free radical species. Results from photocatalytic reactions, in situ transient absorption spectroscopy, and theoretical simulation demonstrate that supported Pd nanoparticles can efficiently stabilize free radical species generated from photo-excited TiO 2 , and thus manipulate their conversion on catalyst surface, owing to the enhanced electronic interactions between metal and radical species. These understandings are crucial for the design of advanced heterogeneous catalytic systems with controllable radical reactions.
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JOURNAL OF SEPARATION SCIENCE
·
2023
·Vol. 46
·期 16
·2300250
·
IF 3.1
YueYue Zhang,Youfang Huang,Xiaojia Huang
Efficient enrichment is a challenging and indispensable step in the quantification of polar synthetic auxins in complex samples. In the current study, a new magnetic adsorbent based on polymeric ionic liquid/aminated carbon nanotube composite was fabricated with a one-pot precipitation copolymerization strategy and employed as the extraction phase of magnetic solid phase extraction of synthetic auxins. Various characterization techniques were utilized to inspect the morphology, structure, magnetic property, and functional groups of the prepared adsorbent. Under the optimal conditions, the obtained adsorbent displayed satisfactory capture performance towards studied auxins through multiple interactions. Adsorption studies evidenced that the adsorption procedure of the developed method towards analytes was fit for the Freundlich adsorption model and pseudo-second-order kinetics. Combining with high-performance liquid chromatography, sensitive and reliable method for the identification and quantification of trace synthetic auxins in environmental water and fruit juice samples was developed. The obtained limits of detection for water and fruit juice samples located in the ranges of 0.0059–0.013 and 0.018–0.031 μg/L, respectively. Recoveries in actual samples with different fortified contents varied from 82.2% to 117%, with satisfactory reproducibility. The results will evidence that the introduced extraction technique is a useful alternative for the entrapment of trace synthetic auxins from complex samples.
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JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2022
·
IF 3.571
Mengmeng Song,Xianjie Sheng,Genyi Ye,Zhuicheng Xu,Xinru Li,Tong Xie,Haishan Deng,An Kang
Gut microbiota-derived tryptophan (TRP) metabolites, especially the indole derivatives, function as critical modulators of intestinal immune function and integrity via modulating the aryl hydrocarbon receptor pathway. Selective enrichment of these indole metabolites in biological samples is important for quantitative determination by routine methods such as HPLC. Here, we report a molecularly imprinted polymer (MIP) for solid-phase extraction (SPE) of TRP-derived indole metabolites from faeces of mice. The MIP was synthesized by surface polymerization by using indole-3-acetic acid (IAA) and acrylamide after cross-linking by ethylene glycol dimethacrylate (EGDMA) and initialed by 2′,2-azobisisobutyronitrile (AIBN). The MIPs were then characterized by transmission electron microscope (TEM) and fourier transform infrared spectroscopy (FTIR), and the adsorption capacity, selectivity and reusability of MIPs were systematically evaluated. Results showed that IAA-MIPs showed a uniformly distributed nanoshell layer with a thin shell thickness of 26 nm . The IAA-MIPs could selectively adsorb IAA, indole-3-propionic acid (IPA), and indole-3-lactic acid (ILA) in a mixed solution that also contains TRP and tyrosine. The adsorption capacity of IAA-MIPs only slightly decreased with the increase of recycling use. As purification material for SPE, the IAA-imprinted polymers (IAA-MIPs) were successfully applied to extract IAA, IPA, and ILA from normal and colitis mice for HPLC determination. Collectively, these surface molecularly imprinted polymers could find extensive use to selective enrichment of microbiota-derived indole metabolites in biological samples.
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JOURNAL OF CHROMATOGRAPHY A
·
2021
·
IF 4.759
Xiaoxia Tian,Yue Dai,Yuanyuan Cheng,Lingdong Zhang,Rong-Mei Kong,Lian Xia,Cong Kong,Guoliang Li
Facile and sensitive determination of plant growth regulators (PGRs) in food samples is important but still remains great challenge. Herein, a pipette tip solid phase extraction (PT-SPE) method was developed for fast and sensitively detecting PGRs. The PT-SPE adsorbent was prepared by integrating a novel covalent organic framework (COF) of schiff base network 3 (SNW-3) and polyacrylonitrile (PAN) through electrospinning. The SNW-3 can easily adsorb PGRs with high special affinity through electrovalent bands between the ammonium ions of SNW-3 and the carboxy groups of PGRs. The polymer of PAN acts as scaffold material for SNW-3, which can lower seepage pressure hence accelerates adsorption/desorption kinetics. By combination with HPLC-DAD, a satisfactory method was successfully developed for simultaneous determination of ten PGRs in watermelon. Good analytical performances were achieved with this proposed method, including good linearity (5–500 ng/mL) with high correlation coefficients ( R ≥ 0.9981), low limits of detection (S/ N = 3, 0.24–3.19 ng/mL) and limits of quantification (S/ N = 10, 1.65–5.72 ng/mL), satisfactory precision (intra-day RSDs ≤ 2.7%, inter-day RSDs ≤ 3.7%), and high accuracy (recovery: 82.8–113.0%). The method developed in this study shows high potential for design of high target-affinity adsorbents for food sample preparing.
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2021
·Vol. 22
·期 24
·13229
·
IF 5.924
Xiaowei Zhang,Xin Fu,Fengjiao Liu,Yanan Wang,Huangai Bi,Xizhen Ai
As an important gas signaling molecule, hydrogen sulfide (H2S) plays a crucial role in regulating cold tolerance. H2S cooperates with phytohormones such as abscisic acid, ethylene, and salicylic acid to regulate the plant stress response. However, the synergistic regulation of H2S and auxin in the plant response to cold stress has not been reported. This study showed that sodium hydrosulfide (NaHS, an H2S donor) treatment enhanced the cold stress tolerance of cucumber seedlings and increased the level of auxin.CsARF5, a cucumber auxin response factor (ARF) gene, was isolated, and its role in regulating H2S-mediated cold stress tolerance was described. Transgenic cucumber leaves overexpressingCsARF5were obtained. Physiological analysis indicated that overexpression ofCsARF5enhanced the cold stress tolerance of cucumber and the regulation of the cold stress response byCsARF5depends on H2S. In addition, molecular assays showed thatCsARF5modulated cold stress response by directly activating the expression of the dehydration-responsive element-binding (DREB)/C-repeat binding factor (CBF) geneCsDREB3, which was identified as a positive regulator of cold stress. Taken together, the above results suggest that CsARF5 plays an important role in H2S-mediated cold stress in cucumber. These results shed light on the molecular mechanism by which H2S regulates cold stress response by mediating auxin signaling; this will provide insights for further studies on the molecular mechanism by which H2S regulates cold stress. The aim of this study was to explore the molecular mechanism of H2S regulating cold tolerance of cucumber seedlings and provide a theoretical basis for the further study of cucumber cultivation and environmental adaptability technology in winter.Keywords:ARF;auxin;cold stress;cucumber;DREB;hydrogen sulfide;module;resistance
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TALANTA
·
2023
·
IF 6.1
Jiangyi Wu,Lingxin Zheng,Xiaojia Huang
Specific extraction and separation plays a pivotal role in the accurate quantification of trace phytohormones (PHs). However, due to their high polarity, specific capture of PHs is challenging. In this study, under the assistance of electric field, a molecular-imprinted-polymer functionalized electrode (MIP@ED) was in-situ prepared using 3-indoleacetic acid (IAA) as template and employed as the adsorbent of electric field-assisted solid-phase microextraction (EA-SPME) for specific capture of PHs. Results showed that the implementation of electric field during the preparation of MIP@ED and EA-SPME procedures improved the extraction selectivity, the selective factors towards IAA and its structural analogues increased from 2.09 to 2.45 to 2.88–3.51. Under the optimum conditions, the proposed MIP@ED/EA-SPME was combined with HPLC technique to monitor trace PHs in water and agricultural products. The achieved limits of detection were in the ranges of 0.0053–0.011 μg/L and 0.048–0.12 μg/kg for water and agricultural product, respectively. The established approach was successfully applied to quantify trace PHs in real samples, and the spiked recoveries varied from 84.0 % to 118 % with good repeatability (RSDs blow 10 %). The obtained results provided clear evidence that the developed approach employing the MIP@ED/EA-SPME technique demonstrated high sensitivity, good selectivity, satisfactory reproducibility and environmental friendliness in the quantification of trace PHs in complex samples. In addition, the current study supplied a new strategy to enhance the specific recognition performance of MIP-based SPME.
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JOURNAL OF HAZARDOUS MATERIALS
·
2023
·
IF 13.6
Jiangping Cao,Lei Shi,Yifei Wang,Yiwei Liu,Baizhao Zeng,Faqiong Zhao
A new ferrofluid extractant (Fe 3 O 4 @SiO 2 -WDES) was ingeniously prepared by coating magnetic Fe 3 O 4 @SiO 2 microspheres with water-based deep eutectic solvent (WDES) and used for dispersive liquid-liquid microextraction (DLLME) and HPLC determination of naphthalene-derived plant growth regulators (PGRs, i.e., 1-naphthylacetic acid, 2-naphthylacetic acid, 1-naphthoxy acetic acid, 2-naphthyloxyacetic acid and 1-naphthylacetamide) in edible oil . Herein, mass transfer of the analytes in DLLME was significantly enhanced via increasing the contact surface by employing the microspheres as the supporter and dispersant of WDES , and phase separation can be efficiently achieved using an external magnet rather than centrifugation in traditional DLLME. Response surface methodology (RSM) based on Box-Behnken design (BBD) was employed for the optimization of core experimental conditions, and Analytical Eco-Scale and Analytical GREEnness Metric Approaches were adopted to evaluate the degree of greenness of the procedure. Under the optimal conditions, satisfactory performances of linearity ranged from 5 to 100 μg/L (R 2 ≥ 0.9982), limit of detection (0.58–0.91 μg/L), limit of quantitation (1.9–3.0 μg/L), precision (RSDs ≤ 5.5%), recovery (81.3%−108.1%) and negligible matrix effect were achieved, which introduced a promising alternative route for the determination of naphthalene-derived plant growth regulators in edible oil.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Shishi Shao,Jiaqiang Zhao,Wei Wang,Hanbing Nie,Yan Jie Li,Xuepeng Zhang,Cheng Zhi Huang,Peng Fei Gao
Host-guest doping room-temperature phosphorescence (RTP) materials have showed excellent potential for ultra-sensitive detection due to its bright luminescence in presence of trace guests. However, the inherent signal fluctuation of solid-state sensors restricts their widespread applications. Herein we constructed a simple internal standard method to overcome the signal fluctuation problem in RTP spectral analysis. Ultralong organic RTP can be obtained by using 1,4-dichlorobenzene (DCB) as a host and trace gaseous NL as a guest through the host-guest doping strategy. In this study, a dual-emission ratiometric phosphorescent probe pyrene (Py)/pentachloropyridine (PCP)-DCB was constructed by using the Py/PCP system fabricated via melt-casting as the reference afterglow and using the DCB as the stimuli-responsive unit of gaseous NL. This probe can be used for visual identification and content detection of NL in commercial mothballs. The ratio of phosphorescence intensity (I485/I595) was linearly related to the mass of added NL (mNL) within the range of 0.05–1.45 mg in this ratiometric RTP platform, and had a low detection limit of 0.012 mg. Furthermore, the NL content of five commercially available mothballs with different NL content was detected by this Py/PCP-DCB platform, and it had good performance compared to the gas chromatography (GC) analysis results.
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JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE
·
2023
·Vol. 148
·期 6
·276-282
·
IF 1.9
Ziying Jiang,Tongrui Liu,Qi Zhang,Lili Dong,Xinyi Deng
The branch number of plants is an important agronomic trait that directly influences the ornamental characters and production costs of ornamental plants. Shoot branching has always been a hot topic for Petunia hybrida. During our research, we isolated the homologous gene of narrow-leaf 1 (NAL1), denoted as PhNAL1. The expression level of PhNAL1 was higher in leaves and axils than in roots, stems, and flowers. Pertinent to shoot apex removal and 6-benzyladenine treatments, both interventions demonstrated a suppressive effect on the expression of PhNAL1. Through subcellular localization analysis, we found that PhNAL1 predominantly localized in the nucleus. By using RNA interference targeting PhNAL1, we induced a noticeable increase in branch number while concurrently reducing plant height of petunia. These findings demonstrate that PhNAL1 is involved in regulating branch development within petunia. This study provides genetic resources for the subsequent cultivation of new cultivars of petunia endowed with distinct branching characteristics.
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Analytical Methods
·
2021
·Vol. 13
·期 26
·2936-2942
·
IF 2.896
Jing Sun,Fang Xue,Cui-Ling Gao,Lei Li,Hai-Long Jiang,Ru-Song Zhao,Jin-Ming Lin
An ionic covalent organic framework (Fe3O4@EB-TFB-iCOF) as a polar adsorbent was synthesized and characterized. It was applied in the magnetic solid phase extraction (MSPE) of four polar organic acids, namely, 2-(2,4,5-trichlorophenoxy)propionic acid, 2-methyl-4-chlorophenoxy acetic acid, naphthyloxyacetic acid, and naphthylacetic acid. The organic acids were detected by high performance liquid chromatography-ultraviolet analysis (HPLC-UV). A method for the determination of organic acids based on MSPE-HPLC-UV was established. The method shows good linear regression (R2 ≥ 0.9950), high precision (1.53–3.80%, n = 6), and low detection limit (0.10–0.49 ng mL−1). The recovery rate of environmental water samples ranges from 73.3% to 101.0%. This method provides a possibility for high sensitivity analysis of polar organic acids.
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