JOURNAL OF SOLID STATE CHEMISTRY
·
2017
·
IF 2.299
Sen Du,Luyan Wang,Ni Xue,Meishan Pei,Weiping Sui,Wenjuan Guo
The modified bentonite with a graft amount of 30% was prepared by polyethylenimine reacting with the epoxy bonds on the molecular of (3-Glycidyloxypropyl) trimethoxysilane and being grafted on the bentonite to remove amino black10B. The effects of pH, time, temperature and concentration on the adsorption capacity were studied. As the pH value increases from 3 to 10, the adsorption capacity decreased from 264.5 to 140.4 mg/g. The adsorption process reached equilibrium at 90 min when pH=3. The adsorption kinetics indicates that the adsorption process was very fast and in accordance with the secondary adsorption kinetics. The data about thermodynamic equilibrium was consistent with the Langmuir model. The adsorption process mainly depended on electrostatic attraction and hydrogen bonding and the adsorption data of Bent-PGLYMO-PEI showed that the maximum adsorption capacity reached 327.7 mg/g.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2016
·Vol. 408
·期 12
·3239-3246
·
IF 3.125
Ma Fangfang,Liang Sheng,Peng Yibo,Kuang Yangfang,Zhang Xing,Chen Shu,Long Yunfei,Zeng Rongjin
Novel fluorescent silver nanoclusters (AgNCs) were synthesized using amido black 10B (AB) as a stabilizing agent and then employed for the detection of copper ions (Cu 2+ ). The AB-stabilized AgNCs (AB–AgNCs) were well dispersed in aqueous solution with an average diameter of around 1.3 nm and exhibited illustrious blue fluorescence emission. Moreover, the fluorescence of AB–AgNCs could be quenched efficiently by Cu 2+ , which might be a result of the coordination between Cu 2+ and the free recognition group of AB on surfaces of AB–AgNCs, inducing the aggregation of AB–AgNCs. Based on the linear decrease of fluorescence intensity, the Cu 2+ concentration was determined in the range of 0.01–1.1 μmol L −1 and the limit of detection (LOD) was 4.0 nΜ. In addition, the detection of Cu 2+ could be performed with AB–AgNCs in the presence of other ions, including 13 kinds of conventional metal ions and 11 kinds of anions. Based on the above experiment, the developed AB–AgNC probe was successfully further applied to detect Cu 2+ in three electroplating effluents, which showed high accuracy.
The process of synthesised silver nanoclusters and the application for Cu 2+ detection.
研究领域:
分析化学
·
环境科学
·
材料科学
✓ 已验证
DOI
高影响因子
Journal of Materials Chemistry B
·
2024
·Vol. 12
·期 25
·6128-6136
·
IF 6.1
Dandan Ding,Shushan Mo,Qishan Li,Fei Wang,Xueyi Wang,Caiwen Ou,Zhenhua Li
Boron neutron capture therapy (BNCT) is an emerging approach for treating malignant tumors with binary targeting. However, its clinical application has been hampered by insufficient 10B accumulation in tumors and low 10B concentration ratios of tumor-to-blood (T/B) and tumor-to-normal tissue (T/N). Herein, we developed fluorinated BPA derivatives with different fluorine groups as boron delivery agents for enabling sufficient 10B accumulation in tumors and enhancing T/B and T/N ratios. Our findings demonstrated that fluorinated BPA derivatives had good biological safety. Furthermore, fluorinated BPA derivatives showed improved 10B accumulation in tumors and enhanced T/B and T/N ratios compared to the clinical boron drug fructose-BPA (f-BPA). In particular, in B16-F10 tumor-bearing mice, fluorinated BPA derivatives met the requirements for clinical BNCT even at half of the clinical dose. Thus, fluorinated BPA derivatives are potentially effective boron delivery agents for clinical BNCT in melanoma.
研究领域:
肿瘤学
·
药理学
·
放射医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ONCOLOGY REPORTS
·
2025
·Vol. 53
·期 5
·1-14
·
IF 3.9
Ying Hu,Xin Liu,Ziheng Yuan,Jianping He,Run Ma,Yuming Wang,Genfa Yi
Globally, lung adenocarcinoma (LUAD) remains the leading cause of cancer‑related mortality, highlighting the urgent need for innovative therapeutic approaches. Necroptosis has been recognized as a crucial mechanism for inhibiting cancer progression. Research has revealed a significant association between microRNA (miRNA)‑mediated necroptosis and tumor progression. The present study aimed to elucidate the role and underlying mechanisms of miR‑10b‑5p in regulating necroptosis in the context of LUAD. In an investigation of LUAD, miRNA sequencing was conducted on both LUAD and adjacent non‑tumor tissues, followed by the integration of external database information to identify specific target miRNAs. The expression of miR‑10b‑5p was verified in LUAD tissues and corresponding adjacent non‑cancerous tissues using immunohistochemistry. In vitro experiments, utilizing LUAD cell lines engineered to modulate miR‑10b‑5p levels, assessed its effects on cellular activities and necroptosis. The inhibition of PKP3 by miR‑10b‑5p was determined using a dual luciferase reporter system. Furthermore, alterations in miR‑10b‑5p levels were found to affect PKP3 expression and inhibit the RIPK3/MLKL signaling pathway, as evidenced by western blot analysis in LUAD cell lines. The effect of PKP3 knockdown on cell activity and necroptosis in LUAD cell lines with low miR‑10b‑5p expression levels was assessed using cell function assays. Finally, a nude mouse xenograft model was used to investigate the effect of miR‑10b‑5p on LUAD growth in vivo and its specific mechanism of action. It has been revealed that miR‑10b‑5p levels are significantly elevated in LUAD specimens. Further investigations demonstrated that an increase in miR‑10b‑5p enhances the proliferation of LUAD cells and suppresses the progression of necroptosis, as evidenced by in vitro experiments. Through dual luciferase reporter assays, PKP3 was confirmed as a direct target negatively regulated by miR‑10b‑5p, leading to reduced expression levels. Western blot analysis indicated that miR‑10b‑5p inhibits the RIPK3/MLKL pathway activation through downregulation of PKP3, which leads to increased cell proliferation and decreased necroptosis. However, knockdown of PKP3 reversed the inhibitory effect of miR‑10b‑5p inhibitors on cellular activity and inhibited necrosis by suppressing the RIPK3/MLKL signalling pathway. In addition, animal model studies demonstrated that inhibition of miR‑10b‑5p activated the RIPK3/MLKL pathway by promoting PKP3 expression and significantly reduced LUAD growth by promoting necroptosis. In conclusion, our studies have revealed that the miR‑10b‑5p functions as a tumorigenic factor, enhancing various cellular activities in LUAD cells and suppressing necroptosis by specifically targeting PKP3, thereby inhibiting activation of the RIPK3/MLKL pathway. Importantly, interventions using inhibitors that specifically target miR‑10b‑5p have shown significant success in impeding the progression of LUAD by promoting necroptosis in both cellular and animal models. Thus, targeting miR‑10b‑5p holds considerable potential as a therapeutic strategy for LUAD.
研究领域:
肿瘤学
·
分子生物学
·
医学遗传学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Plasmonics
·
2017
·Vol. 13
·期 4
·1459-1465
·
IF 2.139
Yu Haili,Wang Zeru,Huang Wei
Here, we report a highly stable and sensitive colorimetric assay for Cr 3+ based on amido black 10B-stabilized silver nanoparticles (AgNPs) as the probes. The detection mechanism is that the coordination interaction between Cr 3+ and amido black 10B on the surface of AgNPs causes the cross-linking/aggregation of amido black-stabilized AgNPs, generating a redshift of the absorption peak and a color change from yellow to pink. Under the optimized assay conditions, this colorimetric assay displays a good linear relationship ( R
2 = 0.996) between relative absorbance ratios and the concentration of Cr 3+ in the range of 0.05–20 μM, and the limit of detection (LOD) was estimated to be 0.01 μM by the UV-Vis spectra and 15 μM by the naked eye. Additionally, the colorimetric assay shows a good selectivity over other metal ions, and it is successfully applied to detection of Cr 3+ in tap water, lake water, and river water samples with satisfactory recoveries.
研究领域:
分析化学
·
环境科学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2016
·
IF 3.138
Pan Hu,Jing Wang,Ruihua Huang
In this study, simultaneous removal of Cr(VI) and Amido Black 10B (AB10B) using quaternized chitosan coated bentonite was investigated by a batch method. The factors affecting adsorption process such as pH, adsorbent dosage, contact time and initial concentration and the interaction of two components in binary solutions have been studied. The results showed that the antagonism effect existed between Cr(VI) and AB10B in binary systems. The equilibrium time for Cr(VI) adsorption was less than the one for AB10B adsorption. The maximum adsorption capacities of the modified bentonite, according to the Langmuir isotherm model were 847.5 mg/g for AB10B and 66.6 mg/g for Cr(VI) at 298 K. The experimental results demonstrated that both Cr(VI) and AB10B were well described by the pseudo-second-order model. Thermodynamic study depicted that the adsorption of Cr(VI) and AB10B onto the modified bentonite was both spontaneous. The adsorption for Cr(VI) was exothermic while the one for AB10B was endoth- ermic in nature.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOCATALYSIS AND BIOTRANSFORMATION
·
2024
·
IF 1.4
Luyao Wang,Qiaoyue Chen,Bingchen Zeng,Yuting Shi,YiLinna YiLi,Chengli Yang,Dali Li
Laccase has a wide range of substrates and the oxidation product is water, providing an ideal choice for the biological degradation and decolorization of dyeing wastewater. This study extracted laccase from the fruiting body of Agaricus bisporus, then used o-phthalaldehyde (OPA) as a cross-linking agent and added surface-aminated Fe3O4 nanoparticles (NH2-nanoFe3O4) to prepare magnetic cross-link enzyme aggregates (MCLEAs) of laccase, which was characterized by scanning electron microscope and vibrating sample magnetometer. The optimal temperature for the catalytic reaction of the MCLEAs was 35 °C, and the optimal pH was 3.0. The stability to temperature pH and storage stability were all enhanced. The immobilized laccase was utilized to treat amido black 10B solution, and the decolorization rate reached 92.48%. After five cycles of use, the MCLEAs of laccase could maintain 42.91% of the relative activity and 32.31% of the relative degradation ability to amido black 10B.
研究领域:
环境生物学
·
酶工程
✓ 已验证
产品关联
DOI
高影响因子
ADVANCED MATERIALS
·
2023
·
IF 29.4
Yucai Zhang,Heon Gyu Kang,Hua-zhen Xu,Honghui Luo,Minoru Suzuki,Qing Lan,Xiao Chen,Naoki Komatsu,Li Zhao
Boron neutron capture therapy (BNCT) has emerged as a treatment modality with high precision and efficacy of intractable tumors. At the core of effective tumor BNCT are 10 B carriers with facile preparation as well as advantageous pharmacokinetic and therapeutic profiles. We herein report the design and preparation of sub-10 nm 10 B-enriched hexagonal boron nitride nanoparticles grafted with poly(glycerol) (h- 10 BN-PG), and their application to cancer treatment by BNCT. By virtue of their small particle size and outstanding stealth property, h- 10 BN-PG nanoparticles accumulate efficiently in murine CT26 colon tumors with a high intratumor 10 B concentration of 8.8%ID/g or 102.1 μg/g at 12 h post-injection. Moreover, h- 10 BN-PG nanoparticles penetrate into the inside of the tumor parenchyma and then are taken up by the tumor cells. BNCT comprising a single bolus injection of h- 10 BN-PG nanoparticles and subsequent one-time neutron irradiation results in significant shrinkage of subcutaneous CT26 tumors. h- 10 BN-PG-mediated BNCT not only causes direct DNA damage to the tumor cells, but also triggers pronounced inflammatory immune response in the tumor tissues, which contributes to long-lasting tumor suppression after the neutron irradiation. Thus, the h- 10 BN-PG nanoparticles are promising BNCT agents to eradicate tumor through highly efficient 10 B accumulation. This article is protected by copyright. All rights reserved
研究领域:
癌症研究
·
纳米技术
·
治疗放射学
·
免疫疗法
✓ 已验证
产品关联
DOI
PubMed
高影响因子
WATER AIR AND SOIL POLLUTION
·
2020
·Vol. 231
·期 6
·1-16
·
IF 1.9
Xie Chunsheng,Wen Xinyi,Xiao Chun,Wei Shoulian,Wu Xiange,Liu Shasha,Cao Jiangfei
Cu-based copper nanoclusters have generated a great deal of interest based on for their fluorescent and catalytic properties. However, as heterogeneous catalysts, little attention has been given to the degradation of organic pollutants in Fenton-like reaction systems. Here, flower-like copper nanoclusters/flower carbon heterostructured microspheres were prepared by a one-pot mixing template-based method. With Cu-based copper nanoclusters/carbon (CuNCs/C) composite as a Fenton-like catalyst, a CuNCs/C-H 2 O 2 system was used to degrade Amido Black 10B (AB-10B). The effects of different factors on the degradation rate of AB-10B were studied. A 98.6% degradation ratio of AB-10B was reached for the CuNCs/C -H 2 O 2 based Fenton-like reaction within 180 min under optimal experimental conditions. The CuNCs/C concentration was 0.06 g/L, the dosage of H 2 O 2 was 25 mmol/L, the mass ratio of CuNCs and the carbon was 1:2, pH = 6.0, and the reaction temperature was 40.0 °C. The removal rate of AB-10B on CuNCs/C decreased by less than 10% even after 6 catalytic cycles, which showed a remarkable reusability characteristic and high catalytic activity. Cu 0 and Cu + were found to co-exist in the catalysts and presented a high degradation effective for intermediates that were identified by liquid chromatography-mass spectrometry (LC-MS). These results indicated that the catalysts can be used as highly efficient Fenton-like catalyst for the degradation of toxic organic pollutants in wastewater.
Graphical Abstract
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
American Journal of Rhinology & Allergy
·
2021
·
IF 2.467
Zhiqi Ma,Haijuan Lian,Xiaoyan Lin,Yong Li
BackgroundAllergic rhinitis (AR) is one of the most common noninfectious respiratory diseases caused by immunoglobulin E (IgE) response.ObjectiveThe study sought to explore the relationship between lncRNA MIAT and miR-10b-5p and their interaction in the regulation of allergic phenotypes in allergic rhinitis (AR) mice.MethodsA mice model of AR was constructed using ovalbumin (OVA) sensitization. AR mice were treated with miR-10b-5p agomiR and LNA mediated lncRNA MIAT. The targeting relationship between MIAT and miR-10b-5p was analyzed by the ENCORI website and dual-luciferase reporter assay. The numbers of rubbing and sneezing of mice were counted. Hematoxylin-eosin (HE) staining visualized the eosinophils infiltration in nasal mucosa tissues of mice. The percentage of Th17 cells was quantitated by flow cytometry analysis. ELISA was used to detect the levels of serum OVA-specific IgE, the Th12 cytokine IL-4, and inflammatory cytokines (IL-6, IL-17).ResultsMIAT was up-regulated in the nasal mucosa of AR mice, while miR-10b-5p was down-regulated. MIAT directly suppressed miR-10b-5p expression in AR mice. The numbers of rubbing and sneezing, the percentage of Th17 cells, and the levels of OVA-specific IgE, IL-4, IL-6, and IL-17 in AR mice were decreased by miR-10b-5p overexpression, which was reversed by MIAT overexpression. The eosinophils infiltration in AR mice was inhibited by miR-10b-5p overexpression, which was also reversed by MIAT overexpression.ConclusionThe present study demonstrates that MIAT overexpression Promotes allergic inflammation and symptoms by activating Th17 immune response via miR-10b-5p inhibition.
研究领域:
免疫学
·
分子生物学
·
过敏
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2018
·
IF 3.927
Yuqi Cui,Xinyi Zhang,Huixuan Zhang,Qingfeng Cheng,Xiuwen Cheng
The application of semiconductor photocatalysts in wastewater treatment has received increasingly attentions because of its potential utilization of solar energy. In this study, a novel visible-light-driven (VLD) BiOCOOH/g-C 3 N 4 composite photocatalyst was successful synthesized by a facile hydrothermal method. Afterwards, physicochemical properties of the resulting samples were investigated through X-ray diffraction (XRD), scanning electron microscope (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), Brunauer-Emmett-Teller (BET) surface areas, UV–visible diffuse reflectance spectroscopy (UV–vis DRS), transient photocurrent density, Mott-Schottky (M−S) and electrochemical impedance spectroscopy (EIS) measurement. Furthermore, the visible light photocatalytic (PC) performance was evaluated through the degradation of amido black 10B dye. All results indicated that the combination of g-C 3 N 4 and BiOCOOH could exhibited greatly visible light absorbance and photo-generated charge separation efficiency, thereby resulting in the enhanced PC performance of BiOCOOH/g-C 3 N 4 composite photocatalyst. The optimal Bi/C molar rate in BiOCOOH/g-C 3 N 4 composite was determined to be 1/1, and the corresponding photodegradation rate for amido black 10B was 0.01794 min −1 , which was nearly 2.19 and 5.73 folds higher than that of pure g-C3N4 and BiOCOOH. The possible mechanism of the PC reaction was also discussed in detail. Furthermore, BiOCOOH/g-C 3 N 4 composite photocatalyst performed good reusability and stability even after four recycle utilization.
研究领域:
环境科学
·
材料科学
✓ 已验证
DOI
高影响因子
TALANTA
·
2022
·
IF 6.556
Qiujun Lu,Lingling Xie,Shuhang Yin,Feng Chen,Cuiyan Wu,Meiling Liu,Haitao Li,Youyu Zhang
The overexpression of microRNA-10b (miRNA-10b) is closely associated with hepatocellular carcinoma (HCC), which may act as an advance biomarker to diagnose HCC early. Here, a novel luminescent nanosensor for the ultrasensitive detection of miRNA-10b was firstly developed by target-triggered catalytic hairpin assembly ( CHA ) and upconversion nanoparticles (UCNPs)-based luminescence resonance energy transfer (LRET). The hybridization process of DNA1-modified UCNPs with DNA2-modified gold nanoparticles (AuNPs) makes the energy donor (UCNPs) and acceptor (AuNPs) into close proximity, resulting in luminescence quenching of UCNPs. The miRNA-10b can hybridize with the hairpin structure of DNA2, releasing the recognition domain site and activate the CHA reaction with its signal amplification chain (H3). At the same time, the LRET was inhibited accompany with the AuNPs moved away from the UCNPs, which resulted in the luminescence recovery of UCNPs. The miRNA-10b can be quantified by measurement of the luminescence recovery rate of UCNPs. The sensor has high sensitivity with a detection limit of 8.96 fM. In addition, the sensor can be used to the discrimination of miRNA-10b expression levels in human hepatocarcinoma and hepatocyte cell lines with satisfactory results. The technique shows great promise for early diagnosis of HCC because of its high sensitivity and selectivity .
研究领域:
生物化学
·
分子生物学
·
医学诊断
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）