METABOLIC ENGINEERING
·
2025
·
IF 6.8
Haixin Gao,Qiang Fang,Yanfen Bai,Chunyue Hu,Howard H. Chou
Biosynthesis of 12-aminododecanoic acid (ADDA) directly from biomass-derived sugars would enable a more sustainable process for manufacturing the engineering polymer Nylon 12. ADDA biosynthesis is currently hindered by the cytotoxicity of dodecanoic acid (DDA) to growing cells, and the accumulation of the overoxidized byproduct dodecanedioic acid (DDDA). In this study, these challenges were addressed by engineering an autoinducible system to better control in vivo DDA synthesis without impacting growth, and deleting aldehyde dehydrogenases and oxidases to reduce DDDA accumulation. As a result, a one-step fermentation process was established to synthesize ADDA from glucose and cellobiose. Finally, batch fermentation achieved 1035 mg/L ADDA and 5% yield, which is the highest titer and yield accomplished directly from sugar to date. This research contributes to the mechanistic understanding of microbial DDA, ADDA, and DDDA synthesis, as well as the goal of developing more sustainable processes for nylon production.
研究领域:
微生物学
·
生物工程
·
合成生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROPOROUS AND MESOPOROUS MATERIALS
·
2022
·
IF 5.876
Shuaiqi Wang,Congxin Wang,Hao Liu,Donge Wang,Xiaoping Wang,Lin Yang,Peng Li,Zhijian Tian
In this work, two ZSM-12 zeolites with stellar morphology and dendritic morphology were synthesized from the synthesis system containing nanorod-aggregated hexagonal prism seed and microrod seed, respectively, in the presence of CTAB. The stellar ZSM-12 zeolite was composed of core and branches, while the dendritic ZSM-12 zeolite consisted of stem and branches. For the two zeolites, the branches grew on {310} planes of seed and were in twin relation with the seed. The c -axes of branch and seed were parallel to each other, while the b -axes of them exhibited the angular relation of 65°. The crystallization process of the two zeolites were investigated by using XRD , SEM, and TEM characterizations. It was demonstrated that the branched growth on seeds was controlled by two factors: diffusion of reactive species and adsorption of CTAB. The Mullins-Sekerka instability occurred under the diffusion-limited condition, resulting in the formation of branches on seed. The adsorption of CTAB on side facets of branches inhibited the occurrence of secondary branching.
研究领域:
材料科学
·
结晶学
✓ 已验证
产品关联
DOI
高影响因子
CHINESE JOURNAL OF CHEMISTRY
·
2017
·Vol. 35
·期 5
·572-576
·
IF 1.852
Qinming Wu,Xiaolong Liu,Longfeng Zhu,Xiangju Meng,Feng Deng,Fengtao Fan,Zhaochi Feng,Can Li,Stefan Maurer,Mathias Feyen,Ulrich Müller,Feng-Shou Xiao
Development of the sustainable routes for synthesis of ITQ-family zeolites is very important because of their unique structures and excellent catalytic and adsorptive properties. The burden of costly raw materials and low efficiency of synthesis put a strong challenge for their widespread commercial application. Here, we show an alternative and simple route for synthesis of ITQ-12, ITQ-13, and ITQ-17 zeolites using commercially available organic templates by a facile grinding process of anhydrous starting raw solids, followed by heating at 140—180 °C. Compared with the conventional hydrothermal synthesis, this approach has obvious advantages such as employment of low-cost organic templates with very high effectiveness, high yield of zeolite products, short crystallization time, and relatively simple procedures. This methodology might open a pathway to synthesize ITQ zeolites with more sustainable manner.
研究领域:
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
Marine Drugs
·
2020
·Vol. 18
·期 7
·375
·
IF 4.073
Mi Zhou,Bo-Rong Peng,Wenjing Tian,Jui-Hsin Su,Guanghui Wang,Ting Lin,Dequan Zeng,Jyh-Horng Sheu,Haifeng Chen
12-Deacetyl-12-epi-scalaradial, a scalarane sesterterpenoid from a marine spongeHippospongia sp, has been reported to possess cytotoxic activity on HepG2, MCF-7, and HCT-116 cells. However, there is no research to indicate that 12-deacetyl-12-epi-scalaradial exhibited anticancer effect on cervical cancer HeLa cells. The aim of this study was to investigate the anticancer activity of 12-deacetyl-12-epi-scalaradial against HeLa cells and to explore the mechanism. The results from a methylthiazolyldiphenyl-tetrazolium (MTT) assay suggested that 12-deacetyl-12-epi-scalaradial suppressed the proliferation of HeLa cells and flow cytometry analysis showed 12-deacetyl-12-epi-scalaradial could induce the apoptosis of HeLa cells in dose- and time-dependent manner. Western blotting analysis demonstrated that 12-deacetyl-12-epi-scalaradial triggered apoptosis via mediating the extrinsic pathway and was found to suppress MAPK/ERK pathway which was associate with cancer cell death. Nur77, a critical number of orphan nuclear receptors, plays diverse roles in tumor development as a transcription factor and has been considered as a promising anticancer drug target. The dual-luciferase reporter assays suggested that 12-deacetyl-12-epi-scalaradial could selectively enhance the trans-activation activity of Nur77. Furthermore, Western blotting analysis and fluorescence quenching showed that 12-deacetyl-12-epi-scalaradial could induce the phosphorylation of Nur77 and interact with the ligand-binding domain (LBD) of Nur77. Our research confirmed 12-deacetyl-12-epi-scalaradial as a potential agent for cervical cancer therapy and provided a view that 12-deacetyl-12-epi-scalaradial may be a modulator of Nur77.Keywords:12-deacetyl-12-epi-scalaradial;HeLa cells;apoptosis;Nur77;MAPK/ERK pathway
研究领域:
海洋化学
·
癌症研究
·
分子生物学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROPOROUS AND MESOPOROUS MATERIALS
·
2021
·
IF 5.455
Shuaiqi Wang,Congxin Wang,Hao Liu,Donge Wang,Lin Yang,Xiaoping Wang,Wei Qu,Huaijun Ma,Zhijian Tian
In this work, the sodium halide , e.g., NaBr, NaCl, or NaF, was added into the synthesis system of ZSM-12 zeolite . The addition of sodium halide accelerated the crystallization of ZSM-12 zeolite, and the acceleration effect of different sodium halide increased in the order of NaBr < NaCl < NaF. To understand the acceleration mechanism of sodium halide, the crystallization process of ZSM-12 zeolite synthesized with and without sodium halide was studied comparatively by using XRD , SEM, TEM , TG and FTIR characterizations. Experimental results revealed that the accelerated crystallization of ZSM-12 zeolite arose from the combined effects of sodium cation and halide anion. The sodium cation induced the coagulation of colloidal particles of initial sol and avoided the formation of gel, resulting in the improved mass transfer. The halide anion promoted the combination of tetraethylammonium cation (as structure-directing agent) with the solid phase. A ZSM-12 zeolite with abundant mesopores was finally obtained from the synthesis system containing sodium halide. The Pt/ZSM-12 catalyst using the mesoporous zeolite as the support exhibited excellent activity and isomerization selectivity in the hydroisomerization of n -dodecane.
研究领域:
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY
·
2016
·Vol. 27
·期 6
·1007-1013
·
IF 1.096
Liu Lijun,Wang Honghong,Gong Shuwen,Lu Jing,Zhang Qian
A novel 12-molybdophosphoric acid (HPM)-based complex (H2PI)2(H3O)[PMo12O40][Mo2O5(H2O)2(PI)2]·11H2O (PI-HPM) was prepared by modification with picolinic acid (HPI) and characterized by the methods of Fourier transform infrared (FTIR) spectroscopy, thermogravimetry (TG), X-ray powder and single crystal diffraction. The complex retained the classical Keggin structure of bulk HPM, there were some strong hydrogen bonds existing between the [PMo12O40]3- polyanion, the [Mo2O5(H2O)2(PI)2] coordination moiety, the protonated HPI and the lattice water molecules. Then PI-HPM was employed as heterogeneous catalyst for esterification reaction to evaluate its acid-catalytic activity. The complex exhibited high activity and good durability in reaction mixtures, indicating that it was a promising heterogeneous acid catalyst for esterification that including the conversion of oleic acid to oleates.
研究领域:
化学
·
催化科学
✓ 已验证
DOI
高影响因子
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
·
2010
·Vol. 27
·期 1
·129-136
·
IF 1.082
Tang Xiaoling,Liu Ji,Wang Bo,Yu Hongwei
Thirty-one ester hydrolases were cloned from Escherichia coli K-12 and an efficient screening strategy was applied to screen and characterize them, emphasizing on their enantioselectivity. We are the first to investigate the enantioselectivity of these enzymes, although their activity had been reported by other researchers. The enzyme XL3 from gene b0349, XL10 from gene b0494, XL15 from gene b3412, XL27 from gene b2154 and XL31 from gene b3825 exhibited high activity towards p -nitrophenyl esters with short chain. The enzyme XL15 from gene b3412 was demonstrated for the first time to show high enantioselectivity to ( R )-1-phenylethyl acetate both in hydrolysis and esterification with enantioselectivity value ( E) > 100 at the conversion of 31.2 and 36.8%, respectively.
研究领域:
生物化学
·
分子生物学
✓ 已验证
DOI
高影响因子
RSC Advances
·
2018
·Vol. 8
·期 48
·27565-27573
·
IF 2.936
Qian Meng,Peng Du,Bo Wang,Aijun Duan,Chunming Xu,Zhen Zhao,Cong Liu,Di Hu,Yuyang Li,Chengkun Xiao
Zirconium modified mesoporous materials were successfully synthesized by different methods including direct synthesis and post synthesis (grafting and impregnating). Meanwhile, the corresponding catalysts were prepared. A series of techniques, including small-angle X-ray scattering (SAXS), transmission electron microscopy (TEM) and pyridine adsorption Fourier-transform infrared (Py-IR), were used to characterize the properties of supports and catalysts. The results of SASX and TEM characterization proved that all the modified materials retained the well-ordered mesoporous structure from the FDU-12 material. N2 adsorption–desorption results showed that the Zr-FDU-12 material obtained by direct synthesis (Zr-in-F) possessed higher specific surface area (709 m2 g−1), pore volume (0.65 cm3 g−1) and larger pore size (18.7 nm) than the Zr-FDU-12 materials obtained by the post synthesis. According to the characterization result of UV-Vis, Zr-in-F exhibited better dispersion of Zr species than materials obtained by the post synthesis. It was found that the incorporation of Zr species not only increased acidities but also enhanced the sulfidities of the Mo species. All the catalysts were evaluated for their activities in the hydrodesulfurization of dibenzothiophene, and the NiMo/Zr-in-F catalyst exhibited the best catalytic performance (97.3%), attributed to its higher specific surface area, larger pore size, higher sulfidity, and more acidic sites.
研究领域:
催化化学
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2016
·
IF 2.653
Chuan-Chuan Zeng,Cheng Zhang,Jun-Hua Yao,Shang-Hai Lai,Bing-Jie Han,Wei Li,Bing Tang,Dan Wan,Yun-Jun Liu
In this article, the in vitro cytotoxicity of platycodin D was evaluated in human PC-12, SGC-7901, BEL-7402, HeLa and A549 cancer cell lines. PC-12 cells were sensitive to platycodin D treatment, with an IC 50 value of 13.5 ± 1.2 μM. Morphological and comet assays showed that platycodin D effectively induced apoptosis in PC-12 cells. Platycodin D increased the levels of reactive oxygen species (ROS) and induced a decrease in mitochondrial membrane potential. Platycodin D induced cell cycle arrest at the G0/G1 phase in the PC-12 cell line. Platycodin D can induce autophagy. In addition, platycodin D can down-regulate the expression of Bcl-2 and Bcl-x, and up-regulate the levels of Bid protein in the PC-12 cells. The results demonstrated that platycodin D induced PC-12 cell apoptosis through a ROS-mediated mitochondrial dysfunction pathway.
研究领域:
药理学
·
细胞生物学
·
肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF POLYMER ENGINEERING
·
2018
·Vol. 38
·期 10
·933-943
·
IF 0.778
Junping Zhou,Ruifang Wang,Xiaojia He,Chunxia Zhao,Haolan Gou,Ling Zhao
The mechanical properties of polybenzoxazine (PBa) resins were improved by adding polyamide 12 (PA12) and modified montmorillonite (OMMT) as additives. The mechanical properties and thermal stability of PBa and resulting PBa composites were investigated using an Instron universal material testing instrument and dynamic mechanical analysis. The morphologies of the fracture surfaces were characterized by scanning electron microscopy. The results of morphological studies showed that PBa/PA12/OMMT composites exhibit significantly improved mechanical properties and thermal stability compared with that of the pristine PBa. When the OMMT content increased to 1 wt%, the fracture toughness (1.36 MPa·m 1/2 ) and the fracture energy ( G IC , 315.76 J·m −2 ) of PBa/PA12/OMMT-1 composites increased by 67.9% and 181.4%, respectively, compared with those of the pristine PBa. The thermal stability properties demonstrated that the storage modulus and glass transition temperature ( T g ) of PBa/PA12/OMMT composites gradually increased with the addition of OMMT particles. The scanning electron microscopy results indicated that PBa/PA12/OMMT composites possess a toughening mechanism of crack deflection, with a large bulk of voids and debonding induced by PA12 and OMMT clay particles. Moreover, the OMMT might provide microvoid nucleating sites at its surface to release constrains for shear yielding.
研究领域:
材料科学
·
复合材料
✓ 已验证
产品关联
DOI
高影响因子
TRIBOLOGY INTERNATIONAL
·
2024
·
IF 6.1
Gongjun Cui,Wenpeng Han,Wuchen Zhang,Junxia Li,Ziming Kou
The aim of this research is to enhance the tribological performance of Stellite 12 coatings at room temperature-1000 °C. To this end, Mo modified Stellite 12 coatings were prepared on the Inconel 718 alloy by laser cladding , and the Mo content was optimized. The effects of Mo on the phases, microstructure and tribological behavior of coatings were investigated. The hardness and tribological performance of coatings were enhanced by adding Mo. There was a threshold content of Mo concerning with tribological performance of Stellite 12 matrix coatings. The 12.0 wt% Mo modified Stellite 12 coating kept more outstanding tribological performance, which was due to the high hardness, in-situ synthesized solid lubricants , friction film and the net structure of complex carbides .
研究领域:
材料科学
·
摩擦学
✓ 已验证
DOI
高影响因子
Nano Select
·
2025
·
IF 3.5
Menglan Hu,Bo Meng,Bingxuan Du,Haichao Li
Carbon micro-embroidered spheres with excellent dispersibility and controllable particle size were prepared by leveraging the intrinsic interaction between the surfactant 3-(N,N-dimethyldodecylammonio) propanesulfonate (SB3-12) and sodium chloride (NaCl). Characterized by SEM and TEM, the overall morphology of the carbon micro-embroidered spheres is similar to that of an embroidered ball, while DLS analysis revealed that their diameters range from 1.5 to 3.5 µm. Further characterization via XRD, Raman spectroscopy, XPS, and FT-IR was conducted on the carbon micro-embroidered spheres. Through these analyses, it was observed that with increasing SB3-12 concentration, the diameter of the carbon micro-embroidered spheres tends to first increase and then decrease; concurrently, disorder and defects in the spheres increase, and the contents of carbon, nitrogen, and oxygen gradually rise. In addition, the optical properties of the carbon micro-embroidered spheres were characterized, exhibiting a broad fluorescence spectral range (375–460 nm). In general, novel carbon micro-embroidered spheres with optical properties were successfully prepared by utilizing the interaction between 3-(N,N-dimethyldodecylammonio) propanesulfonate and sodium chloride.
研究领域:
材料科学
·
纳米技术
·
物理化学
·
胶体与界面化学
·
功能材料
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）