RNA Biology
·
2019
·
IF 5.477
Kang He,Huamei Xiao,Yang Sun,Gongming Situ,Yu Xi,Fei Li
The precise increase and decrease of hormone ecdysone are critical for accurate development in insects. Most previous works focus on transcriptional activation of ecdysone production; however, little is known about the mechanism of switching off ecdysone biosynthesis after ecdysis. Here, we showed that the precursor microRNA-14 (pre-miR-14) encodes two mature miRNAs in silkworm; both of these two mature miRNAs regulate various genes in the ecdysone-signalling pathway. Bmo-miR-14-5p targets on nine genes whereas Bmo-miR-14-3p targets on two genes in the same pathway. These two mature miRNAs increased immediately after the ecdysis, efficiently suppressing the 20-hydroxyecdysone (20E) biosynthesis, the upstream regulation, and the downstream response genes. Knocking down either of two mature miRNAs or both of them delays moult development, impairing development synchrony in antagomir-treated groups. In addition, overexpressing Bmo-miR-14-5p but not Bmo-miR-14-3p significantly affected the 20E titer and increased the moulting time variation, suggesting that Bmo-miR-14-5p, though it is less abundant, has more potent effects in development regulation than Bmo-miR-14-3p. In summary, we present evidence that a pre-miRNA encodes two mature miRNAs targeting on the same pathway, which significantly improves miRNA regulation efficiencies to programmatically switch off ecdysone biosynthesis.
研究领域:
昆虫学
·
分子生物学
·
发育生物学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2024
·Vol. 31
·期 15
·22917-22924
·
IF 0
Liu Xunyue,Zhu Haojie,Song Wenyang,Rao Qiong,Xu Xiaoxiao
Chloramphenicol, a broad-spectrum antibiotic employed for controlling bacterial infections, presents an intriguing aspect in terms of its environmental fate in soils. 14 C-labeled chloramphenicol was used to explore its mineralization and residue characteristics in three distinct agricultural soils in China. The findings revealed a nuanced pattern in the fate of 14 C-chloramphenicol, with notable variations among the different soils under investigation. The chloramphenicol extract residue exhibited a reduction of 18.04% in sandy clay soil, 23.04% in clay loam soil, and 21.73% in loamy clay soil. Notably, the mineralization rate in sandy clay soil was 25.22% surpassed that in the other two soils, particularly during the initial stages of incubation. Over time, the diminishing extract residue underwent conversion into minerals and bound residue. The formation rate of bound residue was increased from 44.59 to 53.65% after adding 10% manure, suggesting that chloramphenicol easily binds with soils rich in organic matter. The bound residue is predominantly localized in the humin fraction across all soils. Additionally, the sterilized soil experiments indicated the pivotal role of microorganisms in influencing the fate of chloramphenicol under the specified experimental conditions. In conclusion, this study offers valuable insights into the environmental dynamics of chloramphenicol in soils, emphasizing the importance of soil composition, organic matter content, and microbial activity. The findings contribute to a scientific understanding of the environmental safety implications associated with chloramphenicol usage.
研究领域:
环境科学
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土壤学
·
微生物学
·
药理学
·
农业化学
✓ 已验证
DOI
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Xiaoyue Zhang,Yue Jia,Nuo Zhang,Dan Wu,Hongmin Ma,Xiang Ren,Huangxian Ju,Qin Wei
The unique optoelectronic and tunable luminescent characteristics of copper nanoclusters (Cu NCs) make them extremely promising as luminophores. However, the limited luminescence intensity and stability of Cu NCs have restricted their application in the field of electrochemiluminescence (ECL). Herein, a self-assembly-induced enhancement strategy was successfully employed to enhance the cathodic ECL performance of flexible ligand-stabilized Cu NCs. Specifically, Cu NCs form ordered sheetlike structures through intermolecular force. The restriction of ligand torsion in this self-assembled structure leads to a significant improvement in the ECL properties of the Cu NCs. Experimental results demonstrate that the assembled nanoscale Cu NC sheets exhibit an approximately three-fold increase in cathodic ECL emission compared to the dispersed state of Cu NCs. Furthermore, assembled nanoscale Cu NCs sheets were utilized as signal probes in conjunction with a specific short peptide derived from the catalytic structural domain of matrix metalloproteinase 14 (MMP 14) as the identification probe, thereby establishing a split-type ECL sensing platform for the quantification of NMP 14. The investigation has revealed the exceptional performance of assembled nanoscale Cu NCs sheets in ECL analysis, thus positioning them as novel and promising signal probes with significant potential in the field of sensing.
研究领域:
纳米技术
·
分析化学
·
生物传感
·
材料科学
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电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ChemistrySelect
·
2022
·Vol. 7
·期 43
·e202202843
·
IF 2.307
Cheng-hui Wang,Qing Tang,Jie Zhao,Xin Xiao,Zhu Tao,Ying Huang
Graphical We have reported a stimuli-responsive supramolecular assembly of twisted cucurbit[14]uril ( t Q[14]) and p -diaminoazobenzene (DAAB). Our research results revealed that the interaction between the DAAB and t Q[14], t Q[14] affords the complex t Q[14]-DAAB. The association and dissociation of the supramolecular assembly could be reversibly controlled by the thermal. Furthermore, the t Q[14]-DAAB complex also responded to selected anionic stimuli such as AcO − and F − , and exhibiting potential analysis application. The supramolecular assembly between twisted cucurbit[14]uril ( t Q[14]) and p -diaminoazobenzene (DAAB) has been successfully developed based on host-guest interactions. Interestingly, as the temperature increases, the assembly dissociates and recovers when the temperature decreases. This shows that the association and dissociation of the supramolecular assembly can be reversibly controlled by the temperature. In addition, the t Q[14]-DAAB complex also exhibits selective ultraviolet and colorimetric responses for AcO − and F − , the detection limits of the sensor for AcO − and F − are 5.17×10 −6 M and 2.31×10 −5 M, respectively. The t Q[14]-DAAB exhibits potential analysis applications.
研究领域:
超分子化学
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分析化学
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产品关联
DOI
高影响因子
Inorganic Chemistry Frontiers
·
2023
·Vol. 10
·期 21
·6193-6203
·
IF 7
Daizong Han,Dongyuan Yang,Chenyao Bi,Guoqing Zhang,Fei Yang,Qingqing Hao,Jianbo Zhang,Huiyong Chen,Xiaoxun Ma
SAPO-14 (AFN topology) zeolite has been considered as an ideal catalyst for the methanol-to-propylene (MTP) reaction with a very high one-pass propylene selectivity, due to its unique framework structure containing narrow 8-MR pore openings and ultra-small AFN cages. However, the conventional synthesis of SAPO-14 by hydrothermal treatments generally suffers from issues relating to low-efficiency, difficulty in product collection, and being unfriendly to the environment. Herein, we showcase a facile strategy for the fast and efficient synthesis of highly crystalline SAPO-14 via dry-gel conversion (DGC) for the first time. The crystallization time can be shortened from 2 days to 8 hours at a lower temperature, and the product yield after 8-hour synthesis can be increased from 41.8 to 71.6% compared with the conventional hydrothermal synthesis. Influencing factors in the synthesis, including the molar ratio of dry gel, the time of crystallization, and the ratio of water-to-dry gel (WTDG), on the crystallinity, morphology, and textural properties of the product zeolites are systematically discussed, and the possible crystallization mechanism is also proposed. Successful attempts using four types of silica sources, including silicic acid, fumed silica, Ludox HS-40, and TEOS, confirm the flexibility and adaptability of the proposed synthesis strategy. The superior catalytic performance with not only a high propylene selectivity (76.2%) but also a large P/E ratio (7.19) demonstrates that the SAPO-14 produced by the DGC method is a high-performance catalyst candidate for the selective conversion of methanol to propylene.
研究领域:
化学工程
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材料科学
·
催化反应
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF PHYSICAL CHEMISTRY B
·
2017
·
IF 3.177
Jing Zhang,Qing Tang,Zhong-Zheng Gao,Ying Huang,Xin Xiao,Zhu Tao
Two supramolecular assemblies between twisted cucurbit[14]uril and hemicyanine dyes have been successfully constructed on the basis of host–guest recognition. These supramolecular assemblies could be reversibly switched under acidic and neutral conditions. Furthermore, they responded to selected chemical stimuli such as methyl violet, thereby exhibiting potential analysis application.
研究领域:
化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BMC BIOLOGY
·
2025
·Vol. 23
·期 1
·1-15
·
IF 4.5
Liu Xinfeng,Wang Tongcheng,Zheng Zeyu,Dong Congcong,Liu Wenyu,Niu Zhimin,Yang Yingbo,Wu Xiaoyun,Qiu Qiang,Yang Yongzhi,Li Kexin,Lenstra Johannes A.,Yan Ping,Kang Minghui,Li Bowen,Fang Lingzhao,Liu Jianquan
Background Animal pigmentation serves as an excellent model for studying genetics, development, and evolution. Among yak breeds, the all-white yak breed ( Bos grunniens ) is the only indigenous variety with pristine white fur, in stark contrast to the black coat color of the wild yak and most domesticated yaks. Results Using whole-genome sequencing data from 387 yaks, we analyzed the population genetic structure of all-white yaks and discovered that they clustered into two distinct genetic groups. Further, by conducting a genome-wide association study (GWAS) based on whole-genome variants (SNPs and indels) between all-white and wild-type yaks, we identified a 14-bp deletion in the promoter of KIT , which decreased its expression in all-white yaks. The following knock-in experiments in mouse confirmed that the absence of the yak 14-bp motif decreases the expression of KIT . Deletion of a human orthologue of the yak 14-bp motif by using the CRISPR/Cas9 system reduces the melanin accumulation in human melanoma cells. Conclusions Overall, our study revealed the genetic basis of all-white yaks and underscored the importance of studying livestock phenotypes to uncover conserved genetic regulators in mammals.
研究领域:
遗传学
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发育生物学
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进化生物学
✓ 已验证
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING SCIENCE
·
2023
·
IF 4.7
Xinhui Liu,Chen Yan,Yilin Wang,Peng Zhang,Siyan Yan,Hanbin Wang,Jianguo Zhuang,Yitao Zhao,Yuchen Wang,Yijun Yu,Qichao Zhao,Xuedong Zhu,Fan Yang
A series of Zn loaded ZSM-5 (Zn/ZSM-5) zeolites were prepared utilizing either Zn(NO 3 ) 2 or Zn-based MOFs (ZIF-61, ZIF-8 and ZIF-14) as Zn sources to explore their effects on the catalysts’ physiochemical properties through characterizations and simulations. Results revealed that hierarchical structures containing meso - to macro- pores of 10 ∼ 80 nm aside from the intrinsic micropores were constructed profiting from the use of Zn-based MOFs. On the other side, lager proportions of medium acid and Lewis acid sites were generated by more Zn(OH) + , whose content was proven to be inextricably linked to the binding energy between Zn and ligand as well as local charge valence state of Zn. Benefitting from hierarchical structures and balanced acidities, Zn/Z5(ZIF-14) employing ZIF-14 as Zn source demonstrated higher aromatic selectivity and longer catalyst lifetime (50.9 % and 26 h) than the microporous counterpart synthesized from zinc nitrate hexahydrate (42.1 % and 20 h) in methanol to aromatics (MTA) reaction.
研究领域:
催化化学
·
材料科学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Wen-Jing Zhong,Chen-Yu Zhang,Jia-Xi Duan,Meng-Rui Chen,Ping-Deng,Bo-Liang Zhang,Nan-Shi-Yu Yang,Han-Xi Sha,Jun Zhang,Jian-Bing Xiong,Cha-Xiang Guan,Yong Zhou
Pulmonary fibrosis (PF) is defined as a specific form of chronic, progressive fibrosing interstitial pneumonia, occurring primarily in older adults with poor prognosis. Alveolar epithelial cell (AEC) senescence is the critical pathological mechanism of PF. However, the molecular mechanisms regulating AEC senescence in PF are incompletely understood. Herein, we provided evidence to support the function of Krüppel-like factor 14 (KLF14), a novel Krüppel-like transcription factor, in the regulation of AEC senescence during PF. We confirmed that the expression of KLF14 was up-regulated in PF patients and mice treated with bleomycin (BLM). KLF14 knockdown resulted in more pronounced structural disruption of the lung tissue and swelling of the alveolar septum, which led to significantly increased mortality in BLM-induced PF mice. Mechanistically, RNA-seq analysis indicated that KLF14 decreased the senescence of AECs by inhibiting endoplasmic reticulum (ER) stress. Furthermore, the pharmacological activation of KLF14 conferred protection against PF in mice. In conclusion, our findings reveal a protective role for KLF14 in preventing AECs from senescence and shed light on the development of KLF14-targeted therapeutics for PF.
研究领域:
肺病学
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分子生物学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry C
·
2024
·
IF 5.7
Zhibin Ye,Ziwang Zhang,Nan Yang,Zhuo Li,Weijie Huang,Jianxin Shi
Artificial light sources have a significant impact on the cultivation of houseplants. In this work, a novel single-phase dual-emission phosphor, Ca18Na3Y(PO4)14:Eu2+,Mn2+ (CNYPO:Eu2+,Mn2+), was synthesized and the emission spectra were modulated by co-doping of Mg2+ to better match the absorption of plants. The occupancies of Eu2+, Mn2+ and Mg2+ are analyzed by using the data of bond length from XRD Rietveld refinements and the emission spectra. The energy transfer process from Eu2+ to Mn2+ is proved through the photoluminescence spectra and decay curves. Benefiting from the efficient energy transfer, two broadband emissions peaking at 500 and 650 nm can be achieved, which meets the requirements of plant growth, and the luminescence colour can be tuned from cyan to white and finally to red by changing the ratio of Mn2+ to Eu2+. The internal quantum efficiency of CNYPO:0.18Eu2+,0.18Mn2+ is 76.8% under 340 nm excitation. The introduction of Mg2+ can regulate the occupation sites of Eu2+ and the emission peak position, improve the responsiveness to the plant absorption spectra, and tune the luminescence colour to white. Finally, a white light-emitting diode (WLED) device fabricated using CNYPO:0.18Eu2+,0.18Mn2+,2.16Mg2+ on a 365 nm chip has a high colour rendering index of 89.0 and a low correlated colour temperature of 3580 K, indicating that the prepared phosphor has potential applications in plant lighting and WLEDs.
研究领域:
材料科学
·
光学工程
·
农业科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MOLECULAR CATALYSIS A-CHEMICAL
·
2014
·
IF 3.679
Sheng-jie Xia,Xiao-bo Zhou,Wei Shi,Guo-xiang Pan,Zhe-ming Ni
Three transition metal oxides (Fe 3 O 4 , CeO 2 and SnO 2 ) modified Zn/Ti–layered double hydroxides (LDHs) were successfully synthesized. These materials were characterized by powder X-ray diffraction (XRD), inductively coupled plasma atomic emission spectrometry (ICP-AES), Brunauer–Emmett–Teller (BET) and UV–vis diffuse reflectance spectrum (UV–vis) for the purpose of confirming the formation of good crystal structure. Comparing with original layered double hydroxides, the transition metal oxides modified Zn/Ti layered double hydroxide materials show narrower band gap, larger surface area, smaller pore volume, pore size distribution, and higher removal efficiency for acid red 14 under visible light. The decomposition efficiency of acid red 14 was all over 92% after 120 min. In addition, the thermal regeneration for re-use of layered double hydroxide materials after photodegradation was feasible for at least four cycles (degradation amount still all over 80%). Furthermore, the kinetic parameters, intermediates and possible photocatalytic pathways for acid red 14 decomposed by these transition metal oxides modified Zn/Ti layered double hydroxide materials were also investigated carefully.
研究领域:
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
Scientific Reports
·
2020
·Vol. 10
·期 1
·1-7
·
IF 3.998
Wang Xueni,Liao Xiuling,Zhang Yimin,Wei Linyao,Pang Yuzhou
Schisandrin B (SchB) is the highest content of biphenyl cyclooctene lignans in Schisandra chinensis . It has been reported to have a variety of pharmacological effects, including anti-inflammatory, anti-oxidant, anti-cancer, heart protection, liver protection. In this study, we found that SchB can promote the proliferation of MC3T3-E1 subclone 14 cells. Meanwhile, we found that SchB can regulate the BMP2-SMADs signaling pathway by increasing gene and protein expression of those relative biomolecules. Furthermore, SchB can raise the RUNX2 and SP7 expression in both mRNA and protein levels. Since the role of BMP2-SMADs-RUNX2-SP7 signaling axis in osteoblast proliferation and differentiation has been well documented. The present experimental findings indicate that SchB could promote the proliferation and differentiation of osteoblasts through BMP2-SMADs-RUNX2-SP7 signaling axis.
研究领域:
药理学
·
细胞生物学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）