Mineralization of hydroxyapatite mineralized by acidic metal oxides has attracted substantial research interest in the research and development of orthopedic substitutes. In this study, Polycaprolactone (PCL) nanofibers with nano-tantalum oxide (Ta 2 O 5 ) were successfully fabricated through electrospinning. The scaffold simulates the mineralization process of hydroxyapatite in simulated body fluid. However, in the process of mineralization, there is a chemical reaction between hydrophobic scaffolds and acid oxides, which is believed to have a significant impact on the biological effects of the scaffolds. The results of SEM, EDS, XRD, Mapping, FTIR, GPC, 13 CNMR, and 1 HNMR explain the principle of hydroxyapatite mineralization by Ta 2 O 5 and the reason why the composite scaffold is blurred, that is, the carbonyl carbon of PCL attacked by the surface hydroxyl groups of Ta 2 O 5 to catalyze its ring-opening polymerization to synthesize PCL/Ta 2 O 5 nanocomposites in one step. In order to accurately explore the effect of this reaction on the metabolism of biological macromolecules in the future, we provide a detailed route of Ag Nanoparticles -Dimercaptosuccinic acid@Transcription activator (AgNPs-DMSA@TAT) probe synthesis. The results provide a reference for the sedimentation of hydroxyapatite induced by acid oxides and its effect on traditional electrospun fiber scaffolds.
Congenital heart disease (CHD) poses significant clinical challenges due to limitations in early prenatal diagnosis. This study aimed to identify serum metabolic biomarkers for CHD using a combined metabolomics approach. Serum samples from 55 pregnant women carrying fetuses with confirmed CHD (CHDP group) and 49 healthy controls (ZCP group) were analyzed via non-targeted 1 HNMR metabolomics, revealing distinct metabolic profiles. Six choline pathway metabolites were further quantified by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Among these, N,N-dimethylglycine (DMG) exhibited the most significant reduction in the CHDP group (1.07 ± 0.03 vs. 1.55 ± 0.04 µg/mL, p < 0.001), with an area under the ROC curve (AUROC) of 0.883 in the discovery cohort. Validation in an independent cohort (58 CHDP vs. 62 ZCP) confirmed DMG’s diagnostic potential (AUROC = 0.818). While betaine-homocysteine methyltransferase 2 (BHMT2) activity showed no intergroup differences, DMG’s consistent performance highlights its utility as a non-invasive biomarker. This study underscores the clinical value of metabonomics in prenatal CHD screening and establishes DMG as a promising diagnostic marker, potentially improving early detection and perinatal management. Graphical abstract
Arginine-functionalized amino flowered mesoporous silica (A-FMS-Arg) nanoparticles are fabricated via the amidation reaction, and taken as efficient sorbent for refined extraction of heparin. Attributed by the multiple H-bond interaction between the sulfate group of heparin and the guanidine group of Arginine, A-FMS-Arg nanoparticles exhibit favorable adsorption selectivity towards heparin. The three-dimensional flowered structure provides A-FMS-Arg with abundant adsorption sites, and the adsorption capacity is high to 173.71 mg/g. 1HNMR assay indicate that A-FMS-Arg nanoparticles can selectively adsorb heparin from the mixture of heparin/chondroitin, and the practicability of A-FMS-Arg is well demonstrated by the efficient extraction of heparin from digested porcine intestinal mucosa.
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