Analytical Methods
·
2018
·Vol. 10
·期 26
·3256-3262
·
IF 2.073
Pan Li,Jiawei Li,Minghong Bian,Danqun Huo,Changjun Hou,Ping Yang,Suyi Zhang,Caihong Shen,Mei Yang
In this paper, we developed a label-free fluorescence assay employing BSA-Au NCs, S2O32−, and 2-ME for the highly selective and sensitive detection of Pb2+ in sorghum, river water and tap water. When the BSA-Au NCs reacted with S2O32− in solution, Au(S2O3)23− complexes were formed immediately on the surfaces of BSA-Au NCs. After adding Pb2+ and 2-ME, the BSA-Au NCs rapidly dissolved to form Au–S complexes. As a result, the fluorescence intensity decreased dramatically, allowing quantification of Pb2+ in the aqueous solution. This reaction is highly specific under optimal detection conditions. A good linear relationship between the fluorescence intensity and the concentrations of Pb2+ could be obtained in the range of 10 to 1000 nM (R2 = 0.9910), with a detection limit (LOD; S/N ratio = 3) down to 1.043 nM. Significantly, compared with other studies, we greatly shortened the reaction time by substantial improvements in materials. On the basis of the above-mentioned work, we successfully immobilized S2O32− modified BSA-Au NCs on wax-printed papers to achieve the purpose of simple and real-time field monitoring of Pb2+. We picked up R-values (the red value of the RGB color model) from captured flurescence images under UV light (365 nm) and acquired a linear correlation between R-values and Pb2+ concentrations. This linear correlation exhibits the great potential of this approach. The proposed probe also showed satisfactory selectivity as well as excellent reproducibility. Multiple real sample experiments showed the tremendous prospect of this method, as well.
研究领域:
分析化学
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环境科学
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食品科学
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DOI
高影响因子
CHINESE JOURNAL OF CHEMICAL PHYSICS
·
2024
·Vol. 37
·期 4
·563-570
·
IF 1.2
Shi Hehang,Ye Xiaodong
Two soy protein 11S fractions with different surface sulfhydryl contents were prepared. Utilizing analytical ultracentrifugation, the effects of storage time and hydrogen peroxide at different concentrations (0.5—100 mmol/L) on the two 11S fractions were investigated. Results show that after removing 2-mercaptoethanol (2-ME) by size exclusion chromatography, the 11S fraction with high surface sulfhydryl content (2.0 mol sulfhydryl/mol 11S) progressively formed 15S and 21S in dilute solutions during storage at 4 °C for 82 days. While, the 11S fraction with low surface sulfhydryl content (0.2 mol sulfhydryl/mol 11S) was stable under the same condition. Moreover, after treating the 11S with high surface sulfhydryl content with 1 mmol/L H 2 O 2 , the weight percentage of 15S reached the maximum value of 20%. The 15S induced by air and H 2 O 2 could be totally converted to 11S with the addition of 10 mmol/L 2-ME, which could be attributed to that the disulfide bond linking two 11S molecules is on the surface of the 15S and easily accessible to the reducing agent 2-ME. This study helps us to deeply understand the formation mechanism of 15S and the stability of 11S.
研究领域:
食品科学
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蛋白质化学
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分析化学
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DOI
高影响因子
JOURNAL OF MEDICINAL CHEMISTRY
·
2024
·
IF 6.8
Yang Zhao,Xinyue Zhao,Lewei Duan,Ruxue Hou,Yuxin Gu,Zhen Liu,Jianbin Chen,Feizhen Wu,Limin Yang,X. Chris Le,Qiuquan Wang,Xiaowen Yan
The success of arsenic in acute promyelocytic leukemia (APL) treatment is hardly transferred to non-APL cancers, mainly due to the low selectivity and weak binding affinity of traditional arsenicals to oncoproteins critical for cancer survival. We present herein the reinvention of aliphatic trivalent arsenicals (As) as reversible covalent warheads of As-based targeting inhibitors toward Bruton’s tyrosine kinase (BTK). The effects of As warheads’ valency, thiol protection, methylation, spacer length, and size on inhibitors’ activity were studied. We found that, in contrast to the bulky and rigid aromatic As warhead, the flexible aliphatic As warheads were well compatible with the well-optimized guiding group to achieve nanomolar inhibition against BTK. The optimized As inhibitors effectively blocked the BTK-mediated oncogenic signaling pathway, leading to elevated antiproliferative activities toward lymphoma cells and xenograft tumor. Our study provides a promising strategy enabling rational design of new aliphatic arsenic-based reversible covalent inhibitors toward non-APL cancer treatment.
研究领域:
癌症研究
·
药理学
·
分子生物学
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药物化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2024
·Vol. 22
·期 1
·1-20
·
IF 10.6
Zheng Wei,Li Shixiong,Shi Zhongliang,Su Kailing,Ding Yu,Zhang Luyue,Tang Qian,Han Jiani,Zhao Han,Wang Fengwei,Zhang Hongru,Hong Zhangyong
Background Tumor neoantigen peptide-based vaccines, systemic immunotherapies that enhance antitumor immunity by activating and expanding antigen-specific T cells, have achieved remarkable results in the treatment of a variety of solid tumors. However, how to effectively deliver neoantigens to induce robust antitumor immune responses remains a major obstacle. Results Here, we developed a safe and effective neoantigen peptide delivery system (neoantigen-ferritin nanoparticles, neoantigen-FNs) that successfully achieved effective lymph node targeting and induced robust antitumor immune responses. The genetically engineered self-assembled particles neoantigen-FNs with a size of 12 nm were obtained by fusing a neoantigen with optimized ferritin, which rapidly drainage to and continuously accumulate in lymph nodes. The neoantigen-FNs vaccine induced a greater quantity and quality of antigen-specific CD8 + T cells and resulted in significant growth control of multiple tumors, dramatic inhibition of melanoma metastasis and regression of established tumors. In addition, no obvious toxic side effects were detected in the various models, indicating the high safety of optimized ferritin as a vaccine carrier. Conclusions Homogeneous and safe neoantigen-FNs could be a very promising system for neoantigen peptide delivery because of their ability to efficiently drainage to lymph nodes and induce efficient antitumor immune responses. Graphical Abstract
研究领域:
癌症免疫疗法
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纳米技术
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免疫学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
ADVANCED MATERIALS
·
2024
·
IF 27.4
Zhuo Li,Boguang Yang,Zhengmeng Yang,Xian Xie,Zhengnan Guo,Jianyang Zhao,Ruinan Wang,Hao Fu,Pengchao Zhao,Xin Zhao,Guosong Chen,Gang Li,Fuxin Wei,Liming Bian
Cellular energetics plays an important role in tissue regeneration, and the enhanced metabolic activity of delivered stem cells can accelerate tissue repair and regeneration. However, conventional hydrogels with limited network cell adaptability restrict cell–cell interactions and cell metabolic activities. In this work, it is shown that a cell-adaptable hydrogel with high network dynamics enhances the glucose uptake and fatty acid β -oxidation of encapsulated human mesenchymal stem cells (hMSCs) compared with a hydrogel with low network dynamics. It is further shown that the hMSCs encapsulated in the high dynamic hydrogels exhibit increased tricarboxylic acid (TCA) cycle activity, oxidative phosphorylation (OXPHOS), and adenosine triphosphate (ATP) biosynthesis via an E-cadherin- and AMP-activated protein kinase (AMPK)-dependent mechanism. The in vivo evaluation further showed that the delivery of MSCs by the dynamic hydrogel enhanced in situ bone regeneration in an animal model. It is believed that the findings provide critical insights into the impact of stem cell–biomaterial interactions on cellular metabolic energetics and the underlying mechanisms.
研究领域:
生物材料
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干细胞生物学
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组织工程
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代谢工程
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再生医学
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产品关联
DOI
PubMed
高影响因子
MACROMOLECULES
·
2024
·
IF 5.1
Nianzhao Gao,Yanglei Zheng,Jianan ShangGuan,Haoran Sun,Junyi Jiang,Shuangfei Xiang,Shujun Zhao,Feiya Fu,Xiangdong Liu
The development of sustainable composites necessitates biobased epoxy resins that are highly recyclable and degradable; however, the integration of mechanical, reprocessing, and rapid degradation properties into a single epoxy resin remains a significant challenge. The present study proposes a straightforward approach to overcoming the problem by combining two labile covalent bonds into an epoxy resin. The combination of acetal and disulfide bonds demonstrates a synergistic effect on the degradation performance of epoxy resin, leading to an ultrafast degradation of the epoxy polymer. The carbon fiber-reinforced composite with the epoxy resin matrix shows a tensile strength exceeding 630 MPa, but the epoxy resin degrades completely within just 8 min, while the recovered carbon fibers display nondestructive characteristics similar to those of the original material. Moreover, the epoxy resin that we designed shows good self-healing and reprocessing ability. Scratches on the resin surface can be completely self-healed by heating, while the powdered resin can be reshaped under a hot press. These findings offer a new approach to the preparation of sustainable composites, highlighting the significant importance of the development of thermosetting polymers.
研究领域:
材料科学
·
可持续发展
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产品关联
DOI
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2025
·
IF 3.5
Mingyue Lu,Jiaying Huo,Xin Liu,Xiongwei Yu,Qinli Fu,Ying Gao,Lulu Ma,Shugang Li
BACKGROUND Egg white protein (EWP) can be used to enhance the thermal gel properties of pumpkin seed protein (PSP), but the underlying molecular mechanism and potential for foods for individuals with dysphagia remain unexplored. This study aims to investigate the texture properties of PSP–EWP co-gelation. RESULTS Egg white protein significantly improved disulfide bond formation (from 31.74% to 41.70%) and promoted hydrophobic group folding within the composite system. These structural modifications enhanced the protein-water binding capacity, as evidenced by a 94.19% increase in bound water content, thus exhibiting marked improvements in hardness (from 27.63 to 599.72 g), springiness (from 28.32% to 87.55%), and water-holding capacity (from 16.06% to 68.04%) of the dual protein composite gel. Multiple techniques, including secondary structure analysis, fluorescence spectroscopy, particle size measurement, surface hydrophobicity assessment, atomic force microscopy (AFM), and scanning electron microscopy (SEM), collectively revealed the mechanism of dual-protein aggregate formation. These aggregated function within the gel system by enabling proteins to regulate aggregation behavior through the synergistic effects of electrostatic interactions and hydrogen bonding. Egg white protein dominated the construction of dense gel network scaffolds in the composite gels, whereas PSP hindered cross-linking through physical obstruction, resulting in a porous structure. When the PSP:EWP ratio was 8:4, 6:6, or 4:8, the International Dysphagia Dietary Standardization Initiative (IDDSI) test showed that the composite gel could be classified into six or seven grades. Such result indicated that the composite gel was suitable for people with mild dysphagia. CONCLUSION The results showed that the properties of PSP–EWP composite gels could be controlled by adjusting the PSP:EWP ratio, indicating the potential use in transitional foods for patients with dysphagia. © 2025 Society of Chemical Industry.
研究领域:
食品科学
·
蛋白质化学
·
生物物理学
·
材料科学
·
营养学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2024
·Vol. 16
·期 16
·2472-2477
·
IF 2.7
Chenhao Zhao,Xingdan Wang,Jing Wu,Yeli Hu,Qiwei Zhang,Qi Zheng
Sialic acids are a family of monosaccharides that share a nine-carbon backbone and a carboxyl group. A recent derivatization method based on 3-nitrophenylhydrazine (3-NPH) provides a mild chemical labeling technique for biomolecules containing carbonyl or carboxyl groups. In this study, we utilized 3-NPH to label sialic acids via a two-step derivatization process. The derivatized species can produce a common reporter ion corresponding to C1–C3 with two labels, and a fragment differentiating between Neu5Ac, Neu5Gc, and KDN. This method is compatible with O-acetylated sialic acids and provides high sensitivity to Neu5Gc and KDN, and since the utilization of dual labeling significantly enhances the hydrophobicity of derivatives, it can effectively mitigate matrix effects when combined with parallel reaction monitoring technology. Negative-ion tandem mass spectrometry (MS/MS) analysis reveals a distinctive fragmentation profile for the 4-O-acetylated species, while the other sialic acids yield similar MS/MS spectra with a high abundance of reporter ions. Using the reporter ion as a transition, this analytical strategy is effective for analyzing complex biological samples. For example, it was successfully employed to quantify sialic acids in the intestinal tissues of several carp species, demonstrating its potential in sialylation research.
研究领域:
分析化学
·
糖生物学
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产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2025
·
IF 5.4
Le Wang,Zhixin Duan,Pengfei Fei,Zhifeng Yan,Wei Wang,Youbo Di,Jianjun Lu
The protease method of wool shrink-proofing finishing is challenging to apply industrially due to its low efficiency and prolonged treatment time. In this study, the activator P[(CH 2 ) n OH] 3 , n∈(1, 10) and protease Savinase 16 L were combined and used in the same bath to rapidly degrade wool scales to achieve the target of machine washable for wool. The activator significantly improved the degradation efficiency of 16 L on wool's high-sulfur scale tissue, reducing the wool shrink-proofing treatment time to 100 s. The treated wool samples' area shrinkage, strength, elasticity, length, fineness, and directional friction effect were evaluated. The results showed that the activator combined with Savinase 16 L could rapidly hydrolyze wool fiber scales, and the wool area shrinkage was 1.65 % without excessive damage to the original properties of wool fibers. Furthermore, the reaction mechanism of wool scale degradation by protease and the activator was confirmed by Raman spectroscopy, X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). The results showed that the activator worked by activating the wool high-sulfur scale layer, thereby increasing the efficiency of the protease degradation of wool. It had no activating effect on the protease molecule. The activator opens the disulfide bonds on the wool scales while transforming the β-thinning and β-rotation in the scales to α-helix or random curling. It could promote protease adsorption and reaction on wool. Under pH 8.0 and 50℃, 2 g/L of activator could increase the adsorption of 16 L on wool by 12.7 times and increase the hydrolysis activity of 16 L on wool by 1575 times. After treatment with this technology, the content of -NH2, -COOH, and -SOx on the surface of wool was increased. Furthermore, a continuous multiple-padding wool shrink-proofing production line was designed, including a treatment liquid circulation and heat preservation systems. Stable industrial production applications were realized.
研究领域:
纺织科学
·
生物化学
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产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 11
·1-14
·
IF 5.3
Liu Yansheng,Sun Yukun,Jia Xiaobo,Zhou Jin,Li Kang,Li Zhaoxu,Wang Guofu
A method for detecting methamphetamine (MET), ketamine (KET), and morphine (MOP) molecules is presented using a reusable substrate based on SERS. The SERS substrate was prepared by etching the Au/Ag alloy film to synthesize a nanoporous Au membrane (AuNPM). By optimizing the preparation conditions and using rhodamine 6G (R6G) as an analyte, the AuNPM exhibited good SERS performance with a limit of detection (LOD) of 10 −9 mol L −1 . A competitive immunoassay category has been applied to the detection of MET, KET, and MOP. The MET, KET, and MOP antigens were functionalized on the surface of the AuNPM to specifically bind to the related drug antibodies. The Au nanoparticles (AuNPs) modified with 4-mercaptobenzoic acid (4-MBA) and antibodies against MET, KET, and MOP were used as nanotags. The 4-MBA served as the reporting molecule and drug antibodies were used to bind to free drug molecules in the target solution. The mixture of nanotags and target solution was dropped onto the antigen-modified AuNPM (antigen/AuNPM), and the free nanotags bind to the antigen/AuNPM. By comparing the SERS intensity of 4-MBA with the presence or absence of drug molecules, the drugs were qualitatively and quantitatively identified. Through this category, the LODs for detecting MET, KET, and MOP were 0.1, 1, and 1 ng mL −1 , respectively. This study proposes an effective method for constructing SERS-based detection of drug molecules with good potential for practical applications. Graphical abstract
研究领域:
分析化学
·
药物检测
·
纳米材料
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Kai Han,Jian Liu,Fukang Hao,Jianlong Wang,Jinfeng Yuan,Zhicheng Pan,Mingwang Pan
Waterborne fluorocarbon coatings are gaining popularity as a more environmentally friendly replacement for traditional solvent-based coatings because of its corrosion resistance and antibiofouling properties. However, the environmental unfriendliness of small molecular emulsifiers used, the occurrence of flash rusting, and the inability to self-repair after accidental damage restrict its application in the marine industry. Here, a redox-responsive copolymer (PM x ) was synthesized by the amphiphilic nature of waterborne polyurethane as microreactors, which was employed as coatings (PM x /WPU) with anti-flashing rust, antibiofouling, and self-repairing functions. The copolymer was polymerized from chlorotrifluoroethylene (CTFE), vinyl acetate (VA), and a special monomer conjugated to the corrosion inhibitor 2-benzothiazolethiol (MBT) through disulfide bonds, in which MBT can be released through disulfide bond breaking in a reducing environment, effectively preventing the occurrence of flash rusting. The combination of the repelling properties of the fluorinated groups and the bactericidal action of the MBT results in PM x /WPU coatings with excellent antibiofouling properties. Additionally, the reversible hydrogen bonds in the polyurethane provide the PM x /WPU coatings with excellent self-repairing ability (93.9%). Our study presents a new approach to polymerize fluoro-substituted ethylene monomers using polyurethane as microreactors, enabling the preparation of waterborne fluorocarbon coatings with anti-flashing rust, antibiofouling, and self-repairing properties for practical applications.
研究领域:
材料科学
·
海洋工程
·
化学工程
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Diandian Huang,Lingyuan Wang,Keting Li,Lingyi Liu,Xingyi Chen,Laping He,Lei Wang,Angxin Song
Curcumin is a natural lipophilic polyphenol that exhibits significant various biological properties such as antioxidant and anti-inflammatory properties following oral administration. However, its uses have shown limitations concerning aqueous solubility, bioavailability and biodegradability that could be improved by prolamin-based nanoparticle. In this study, curcumin was encapsulated into prolamin from sorghum (SOP) and wheat (WHP) and distilled spirit spent grain (DSSGP), which was obtained after microbial proteolysis of the former two cereal grains. All the three prolamins showed clear variation of protein profiles and microstructure as confirmed by electrophoresis analysis, disulfide bond determination and Fourier-transform infrared spectroscopy (FTIR). For curcumin-loaded nanospheres (NPs) fabrication, three prolamin-based NPs shared features of spherical shape, uniform particle size, and smooth surface. The average size ranged from 122 to 193 nm depending on the prolamin variety and curcumin loading. In the experiments in vitro, curcumin showed significantly improved UV/thermal stability. Furthermore, DSSGP was more resistant to enzymatic digestion in vitro, hence achieving the controlled release of curcumin in gastrointestinal tract. Collectively, the results indicated the improved bioavailability and biodegradability of curcumin encapsulated by DSSGP, which would be an innovative potential encapsulant for effective protection and targeted delivery of hydrophobic compounds.
研究领域:
食品科学
·
生物技术
·
药学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）