LANGMUIR
·
2024
·
IF 3.7
Xue Chen,Yong Xiang,Xiaojing Zhang,Guanlin Li,Sihan Ai,Daoyong Yu,Baosheng Ge
Carbonic anhydrase (CA) plays a crucial role in the CO2 capture processes by catalyzing the hydration of CO2. In this study, we synthesized a bioinspired carbonic anhydrase Zn-MOF (metal–organic framework) incorporating 2-aminoimidazole and Zn2+ as initial constituents. The synthesized Zn-MOF exhibited promising potential for efficiently catalyzing the CO2 hydration. Structural analyses such as SEM, XRD, and BET confirmed that the Zn-MOF crystal consisted of stacked grains with an average size of approximately 36 nm, forming a micron-sized spherical structure. Functionally, Zn-MOF exhibited effective catalytic activity toward both CO2 hydration and ester hydrolysis. The introduction of amino groups significantly enhanced the esterase activity of Zn-MOF to 0.28 U/mg at ambient temperature, which was twice that of ZIF-8. Furthermore, the introduction of amino groups resulted in remarkable hydrothermal stability, with the esterase activity reaching 0.72 U/mg after undergoing hydrothermal treatment at 80 °C for 12 h. Additionally, Zn-MOF exhibited enhanced capability in CO2 hydration at a pH value exceeding 8.5. After six repeated uses, ZIF-8 and Zn-MOF retained approximately 68 and 65% of their initial enzyme activity, respectively, underscoring the potential practical applicability of Zn-MOF in industrial CO2 capture processes. This work showcases the development of a novel Zn-MOF crystal as an efficient CA mimic, effectively emulating the active sites of natural CA using 2-aminoimidazole as a coordinating ligand for Zn2+ coordination. These findings not only advance the field of innovative enzyme mimics but also pave the way for further exploration of industrial CO2 capture catalysts.
研究领域:
材料科学
·
生物化学
·
环境科学
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产品关联
DOI
PubMed
高影响因子
LANGMUIR
·
2026
·
IF 4.4
Yong Xiang,Xue Chen,Liang Zhang,Yu Chen,Xiaowei Peng,Zhongzhen Cai,Qiping Gao,Daoyong Yu,Lu Zhang,Baosheng Ge
Metal–organic frameworks (MOFs) show great promise as carbonic anhydrase (CA) mimics due to their high surface area, tunable porosity, and abundant active sites. This study enhances a reported 2-aminoimidazole-based Zn-MOF by incorporating Co2+, Ni2+, or Cu2+ via in situ doping, systematically investigating how metal doping regulates morphology, crystal structure, and catalytic performance. The results reveal that the type and ratio of doped metal ions critically influence the framework: Co2+ demonstrated the highest compatibility, while Ni2+ and Cu2+ induced structural distortion at higher levels. All doped samples exhibited reversible catalytic kinetics akin to natural CA, with improved maximum reaction rates (Vmax) and substrate affinity. Specifically, Co75%/Zn-MOF, Ni50%/Zn-MOF, and Cu25%/Zn-MOF achieved optimal performance, showing esterase activities of 0.47 ± 0.02, 0.56 ± 0.02, and 0.39 ± 0.02 U/mg─increases of 67.9%, 100.0%, and 40.0% over pristine Zn-MOF, respectively. These materials also displayed exceptional high-temperature (80 °C) activity, hydrothermal stability, pH tolerance, and recyclability, retaining 67–79% activity after six cycles. This work provides a theoretical and experimental basis for designing efficient and stable MOF-based CA mimics, highlighting their potential for carbon capture, utilization, and storage (CCUS) applications.
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2018
·
IF 2.746
Wu Zou,Fuchun Gong,Tingting Gu,Zhong Cao,Jiaoyun Xia
A facile approach was developed to prepare fluorescent nitrogen-rich quantum dots (NCQDs) using 2-azidoimidazole and ammonium hydroxide as reactants. The nitrogen and carbon elements of NCQDs form the frameworks of their nanostructures together and bear multiple active sites. By using NCQDs as fluorophores and graphene oxide (GO) as controllers, a hydrogen binding-based nanosensor was constructed for the detection of pyrophosphate (PPi). In NCQDs-GO sensing assemblies, the binding of GO to NCQDs results in the fluorescence quenching by FRET mechanism; while the addition of PPi leads to the fluorescence recovery of NCQDs because of the competitive binding between NCQDs and PPi to GO. The fluorescence intensity showed linearity with the added PPi concentration ranging from 0 to 400 μM with the detection limit of 8.3 nM (σ = 3). This procedure for the fabrication of amino-functionalized carbon quantum dots is simpler and more efficient compared to conventional doping and surface modification methods. The proposed NCQDs-GO sensing assemblies showed high selectivity and sensitivity to PPi over the competing anions in aqueous solution.
研究领域:
生物化学
·
分析化学
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产品关联
DOI
高影响因子
Small
·
2025
·
IF 12.1
Yujie Guo,Lingfei Li,Maofeng Zhang,Shihao Xu,Changlong Jiang
Polymetallic MOFs heterostructural composites enhance their usage in a range of sensing domains by combining two or more different MOFs to produce a new MOF-on-MOF integrated module. As its widespread utilization of flavomycin (Fla), there are currently complications with agricultural residue and the environment, consequently, a highly sensitive, portable technology for identification will be required to handle environmental monitoring in real-time. In this work, the structural characterization and characteristics of the two-layer MOF-on-MOF heterostructure are investigated using the epitaxial growth method. Electrons migrate from the Fe metal center to the organic ligand primarily as a result of hydrogen bonding between Fla and the surface of Fe-MOF and energy transfer amongst the Fe atoms and NH 2 -MIL-101 (Eu). The synergistic impact of NH 2 -MIL-101(Eu) and Fe-MOF improves the sensitivity and accuracy of detection, which has a 4.8 n m detection limit, enabling the high sensitivity detection of Fla. Likewise, we put together a handheld sensing platform incorporating aerogel and sensing materials that can meet the expectations of real-time monitoring and multiple actual sample detection, and fluorescent MOFs materials offer an innovative viewpoint on the application of creating flexible sensors, enabling a new method of visualization for food safety and environmental early alerting.
研究领域:
材料科学
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环境科学
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食品安全
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产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2022
·Vol. 46
·期 44
·21235-21244
·
IF 3.925
Huan Xu,Mingyue Yang,Yijing Du,Tian Gao,Yi Liu,Liwei Xiong,Na Peng
Nano-drug carriers have been widely used in chemotherapy to improve the efficacy of chemotherapeutic drugs. Polymer nano-carriers have greater potential for the enrichment of chemotherapeutic drugs at the target tumor site and controlled release. Herein, a series of amphiphilic copolymers (ITT) was synthesized via melt polymerization with a ROS-cleavable thioketal cross-linker, imidazole groups, and polyethylene glycol-conjugated triphenylphosphonium (PEG-TPP) segments; the actual proportions of the imidazole groups to the PEG-TPP segments were 5.2 : 1, 2.6 : 1, and 1.8 : 1, respectively. The amphiphilic copolymers could self-assemble into cationic micelles with hydrophobic imidazole groups as the core and hydrophilic PEG-TPP segments as the shell in deionized water. The cationic micelles with an average particle size of 124.6–187.3 nm showed a drug loading content (DLC) of 5.65–6.10%. In vitro release studies revealed the pH/ROS dual-responsiveness of the DOX-loaded ITT micelles, and the release behavior of DOX from the micelles strongly depended on the content of imidazole groups in the ITT micelles. Typically, the release of the encapsulated DOX was 27.2%, 30.0%, and 34.1% from DOX-loaded ITT-1 micelles in PBS (pH 6.5) containing 0, 100, and 500 μM H2O2, respectively. The release of the encapsulated DOX was 20.3%, 27.2%, and 42.5% from DOX-loaded ITT-1 micelles in PBS (pH 7.4), PBS (pH 6.5), and PBS (pH 5.0), respectively. The DOX-loaded micelles effectively inhibited the growth of 4T1 cells through being internalized into the 4T1 cells and releasing DOX into the mitochondria, with a cell viability of less than 65% when the DOX concentration was 2.5 μg mL−1. This work presents a novel mitochondria-targeting and pH/ROS-responsive polymer nanocarrier for potential chemotherapy applications.
研究领域:
药剂学
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肿瘤学
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纳米医学
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产品关联
DOI
高影响因子
Small
·
2023
·Vol. 19
·期 16
·2206124
·
IF 13.3
Futing Yang,Wenjie Yu,Qiying Yu,Xiyu Liu,Chunping Liu,Chong Lu,Xinghua Liao,Yi Liu,Na Peng
The apoptosis-resistant mechanism of photodynamic therapy (PDT) usually results in limited therapeutic efficacy. The development of new strategies for sensitizing targeted ferroptosis that bypass apoptosis resistance is of great significance to improve the antitumor efficacy of PDT. In this study, a novel amphiphilic copolymer whose main chain contains reactive oxygen species (ROS)-responsive groups and the end of side chains contains triphenylphosphine is synthesized, to encapsulate porphyrinic metal–organic framework PCN-224 via self-assembly which are hydrothermally synthesized by coordination of zirconium (IV) with tetra-kis(4-caboxyphenyl) porphyrin, and loaded carbon monoxide releasing molecule 401 (CORM-401) by their hollow structures (PCN-CORM), and finally, surface-coated with hyaluronic acid. The nanosystem can sequentially localize to mitochondria which is an important target to induce apoptosis and ferroptosis in cancer cells. Upon excitation with near-infrared light, PCN-224 is activated to produce amounts of ROS, and simultaneously triggers the rapid intracellular release of CO. More importantly, the released CO can sensitize ferroptosis and promote apoptosis to significantly enhance the antitumor efficacy of PCN-224 both in vitro and in vivo. These results illustrate that the mitochondria-targeted drug delivery system combined PDT with CO leads to an effective antitumor efficacy, which maybe a promising way to enhance the treatment efficiency of PDT.
研究领域:
药学
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生物医学工程
·
肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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