JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
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2013
·Vol. 27
·期 5
·272-278
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IF 1.596
Hongxu Yang,Wei Song,Mingyang Jing,Rutao Liu
By utilizing multispectrosopic techniques, the toxic interaction of 2-aminoanthraquinone (2-AAQ) with calf thymus deoxyribonucleic acid (ctDNA) was investigated in vitro under simulated physiological conditions. The experimental results proved that 2-AAQ has a toxic interaction with ctDNA. The binding capacity of DNA with 2-AAQ is diminishing as the pH value of system increasing in the optimization of experimental condition. Moreover we selected pH 7.4, which is nearly physiological condition to enhance the practical significance. According to the Stern–Volmer equation, the quenching was the static quenching process. And the quenching constant can be derived from the fluorescence quenching spectrogram. Ultraviolet absorption spectra and the change in the fluorescence intensity at different ionic strengths further indicated that there was electrostatic binding between 2-AAQ and ctDNA. The circular dichroism experiment showed that the DNA conformation varied from B to A conformation. The basic group enhanced after 2-AAQ embedding. The double helix is more compact, and the DNA conformation changes. © 2013 Wiley Periodicals, Inc. J BiochemMol Toxicol 27:272-278, 2013; View this article online at wileyonlinelibrary.com . DOI 10.1002/jbt.21487
研究领域:
生物化学
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分子毒理学
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药物相互作用
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DOI
PubMed
高影响因子
TALANTA
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2019
·
IF 4.916
Tian Jin,Yue Zhang,Yilei Li,Wenjie Jing,Yunchao Li,Louzhen Fan,Xiaohong Li
Due to severely overlapping emission and or/ excitation spectra of polycyclic aromatic hydrocarbons (PAHs), the specifically recognizing PAHs by the fluorescent technique is still a challenge. Here, synthesized Ag@SiO 2 nanoparticles with 40 ± 4 nm Ag core and 7 ± 1 nm SiO 2 shell performing as a nanoprobe could selectively recognize 2-aminoanthracene (2-AA) via metal-enhanced fluorescence (MEF) within 2 min. The sensing mechanism was based on the facts: (1) there was a perfect spectral overlap between the local surface plasmon resonance (LSPR) peak of Ag@SiO 2 and the absorptive peak of 2-AA, and (2) 2-AA could interact with Ag@SiO 2 nanoparticles through hydrogen bonds between –NH 2 in 2-AA and –OH in SiO 2 shell. As a result, with Ag@SiO 2 nanoparticles as a nanoprobe, a fluorescent assay for detecting 2-AA was developed with a linear range from 1 nM to 800 nM, which exhibited an excellent selectivity over possible PAHs, dyes and metal ions . The detection limit was 1 nM. Finally, the developed assay was applied to analyse 2-AA in industrial wastewater samples, which were highly consistent with that of high performance liquid chromatography (HPLC). This study presents a feasible assay for detecting 2-AA for environmental monitoring and toxic evaluating.
研究领域:
环境科学
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分析化学
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DOI
PubMed
高影响因子
RSC Advances
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2024
·Vol. 14
·期 30
·21432-21438
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IF 3.9
Yunkai Sun,Xiaoxia Wu,Pei Zuo,Zhao Liu,Xuepei Miao,Jian Liu,Hairuo Wen
In the technical route for the synthesis of avanafil, 1-ethyl-(3-dimethylaminopropyl)carbamyldiimide hydrochloride (EDCI) and 1-hydroxybenzotriazole (HOBT) are used as reactive acid–amine binding agents. HOBT contains trace amounts of hydrazine residue, and there is a risk of introducing potentially mutagenic impurities with hydrazide-containing structures. The potentially genotoxic impurities E (Imp-E) and F (Imp-F) of avanafil with altering hydrazide-structure were synthesized by chemical method; subsequently, the impurities were evaluated and classified according to ICH M7 guidelines. Two complementary quantitative structure–activity relationship (QSAR) evaluation systems (Derek and Sarah) based on expert rules and statistics were used to preliminarily predict the genotoxicity of Imp-E and Imp-F, and the prediction result of E was suspected to be positive. In the Ames test of Imp-E and Imp-F, in the dose range of 62.5–1000 μg per plate, with or without the presence of metabolic activation system S9, the number of revertant colonies did not exceed 2 times the number of colonies in the solvent control group and did not show a dose–response relationship, and the test results were negative. Imp-E and Imp-F were determined to be negative for genotoxicity, which could be controlled as class 5 in ICH M7, that is, non mutagenic impurity.
研究领域:
药物化学
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毒理学
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制药工程
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产品关联
DOI
PubMed
高影响因子
Drug Testing and Analysis
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2022
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IF 3.234
Te Xu,Zhen-Yu Niu,Jing Xu,Xin-Duo Li,Quan Luo,An Luo,Yi-Lan Huang,Xing-Tao Jiang,Ze-Hong Wu
Use of electronic cigarettes (e-cigarettes) has increased significantly over the past decade due to consumer perception that these products represent a less risky alternative to combustible cigarettes. E-liquids generally contain a simple mix of vegetable glycerin, propylene glycerol, nicotine, organic acids, and flavourings. Regulators require that harmful and potentially harmful constituents (HPHCs) that might cause harm to the consumer must be monitored in the aerosol generated by e-cigarettes and in cigarette smoke (CS). To quantify HPHCs in aerosols from commercial flavoured e-cigarettes in Chinese market, this study has systematically compared levels of HPHCs, including eight carbonyls, five volatile organic compounds, four tobacco-specific nitrosamines, 16 polycyclic aromatic hydrocarbons, and seven heavy metals, in the aerosols of four market-leading flavoured e-cigarettes and mainstream CS, alongside in vitro cytotoxicity and mutagenicity assays. The vast majority of HPHCs were either undetected or significantly lower in the e-cigarette aerosols than in commercial CS or reference CS (3R4F). Where HPHCs were detected, there were small variations among the different flavoured e-cigarettes. In the neutral red uptake and Ames assays, aqueous extracts of the e-cigarette aerosols did not induce obvious cytotoxicity or mutagenicity, whereas CS aqueous extract showed dose-related cytotoxicity and mutagenicity. Collectively, these results indicate that use of e-cigarettes might potentially lead to a significant reduction in exposure to harmful substances, with fewer cytotoxic and mutagenic effects, as compared with conventional smoking. Further studies based on human puffing conditions and longer evaluation periods will be needed to substantiate this potential.
研究领域:
毒理学
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公共卫生
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烟草控制
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化学分析
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）