查找使用阿拉丁产品的科研文献

Enrichment of polychlorinated biphenyl 28 from aqueous solutions using Fe3O4 grafted graphene oxide

Shaolin Zeng,Ning Gan,Rebecca Weideman-Mera,Yuting Cao,Tianhua Li,Weiguo Sang

In this paper, Fe 3 O 4 nanoparticle (Fe 3 O 4 NPs) grafted graphene oxide (Fe 3 O 4 @GO), are successfully synthesized and used for the extraction of 2,4,4′-trichlorobiphenyl (PCB 28) from a large volume of water solution. With the magnetic solid-phase extraction (MSPE) technique based on the Fe 3 O 4 @GO sorbents, it requires only 30   min to extract trace levels of PCB 28 from 200   mL water samples. The Fe 3 O 4 @GO was analyzed by using powder X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier Transform infrared (FT-IR) spectroscopy, and Vibrating sample magnetometer (VSM), specific surface area analyzer. The adsorption kinetics, adsorption capacity of the adsorbent, and the effect of the solution pH and desorption conditions on the removal efficiency of PCB 28 were investigated. The second-order kinetic equation best describes the sorption kinetics. The results showed that Fe 3 O 4 @GO was a suitable material in the pre-concentration and immobilization of PCB 28 from large volumes of aqueous solutions in polychlorinated biphenyl pollution cleaning.

Green ternary composite of graphitic carbon nitride/TiO2/ polyorthoanisidine for the enhanced photocatalytic treatment of Direct Red 28 for industrial water treatment solutions

Ali S. Alkorbi,Nouman Gill,Muhammad Naveed Anjum,Muhammad Jawwad Saif,Mirza Nadeem Ahmad,Muhammad Bilal Qadir,Zubair Khaliq,Mohd Faisal,Mohammed Jalalah,Farid A. Harraz

Industrial dye effluent causes significant risks to the environment. The present study was focused on photocatalytic degradation of the dye Direct Red 28 using a ternary composite of graphitic carbon nitride, TiO2, and polyorthoanisidine (g-C3N4/TiO2/POA), prepared by in-situ oxidative polymerization o-anisidine. The synthesized composite g-C3N4/TiO2/POA properties were characterized using different analytical techniques. X-ray diffraction (XRD) results revealed the prominent pattern of TiO2 and g-C3N4 in the composite peak at 2θ° while Fourier transform infrared (FTIR) results provided the confirmation peaks for g-C3N4/TiO2/POA and POA at 1,110 cm−1 and 1,084 cm−1 for C-O-C ether. Scanning electron microscopy (SEM) demonstrated an increase in the average size of the composite up to 428 nm. The energy-dispersive X-ray spectroscopy (EDX) spectrum provided the weight percentages of the C, O, and Ti in the composite were 8.5%, 45.69%, and 45.81%, respectively. The photocatalytic degradation of Direct Red 28 dye under UV irradiation using a composite showed that 86% Direct Red 28 dye was degraded by a 30 mg/L dose of g-C3N4/TiO2/POA in 240 min at pH 2. After four consecutive cycles, the utilized composite showed 79% degradation of Direct Red 28, demonstrating the stability and effectiveness of the g-C3N4/TiO2/POA photocatalyst. The high reusability and efficiency of the g-C3N4/TiO2/POA composite are due to increased light absorption range and reduced e−/h+ recombination rate in the presence of g-C3N4 and POA.

Toxicity induced by Basic Violet 14, Direct Red 28 and Acid Red 26 in zebrafish larvae

Bing Shen,Hong-Cui Liu,Wen-Bin Ou,Grant Eilers,Sheng-Mei Zhou,Fan-Guo Meng,Chun-Qi Li,Yong-Quan Li

Basic Violet 14, Direct Red 28 and Acid Red 26 are classified as carcinogenic dyes in the European textile ecology standard, despite insufficient toxicity data. In this study, the toxicity of these dyes was assessed in a zebrafish model, and the underlying toxic mechanisms were investigated. Basic Violet 14 and Direct Red 28 showed acute toxicity with a LC 50 value at 60.63 and 476.84 µg ml –1 , respectively, whereas the LC 50 of Acid Red 26 was between 2500 and 2800 µg ml –1 . Treatment with Basic Violet 14, Direct Red 28 and Acid Red 26 resulted in common developmental abnormalities including delayed yolk sac absorption and swimming bladder deflation. Hepatotoxicity was observed in zebrafish treated with Basic Violet 14, and cardiovascular toxicity was found in zebrafish treated with Acid Red 26 at concentrations higher than 2500 µg ml –1 . Basic Violet 14 also caused significant up-regulation of GCLC gene expression in a dose-dependent manner whereas Acid Red 26 induced significant up-regulation of NKX2.5 and down-regulation of GATA4 at a high concentration in a dose-dependent manner. These results suggest that Basic Violet 14, Direct Red 28 and Acid Red 26 induce developmental and organ-specific toxicity, and oxidative stress may play a role in the hepatotoxicity of Basic Violet 14, the suppressed GATA4 expression may have a relation to the cardiovascular toxicity of Acid Red 26. Copyright © 2015 John Wiley & Sons, Ltd.

miR-28-5p targets MTSS1 to regulate cell proliferation and apoptosis in esophageal cancer

Zhang Liang,Wang Xin,Liu Xin,Lv Mingyue,Shen Erdong,Zhu Guolian,Sun Zhe

Esophageal cancer (EC) is one of the most common aggressive malignant diseases worldwide. miR-28-5p plays important regulatory roles in many cancers including human EC. However, the molecular mechanism and potential role of miR-28-5p in EC remain uncertain. In this study, qRT-PCR and western blot analysis revealed that miR-28-5p expression was up-regulated and metastasis suppressor-1 (MTSS1) was down-regulated in EC tissues relative to matched para-cancer tissues. Cell counting kit-8 (CCK-8) assay demonstrated that miR-28-5p mimics increased cell viability, and miR-28-5p inhibitor decreased it. Flow cytometry (FCM) assay indicated that miR-28-5p mimics promoted cell cycle entry, while miR-28-5p inhibitor reduced it and induced cell apoptosis. Moreover, miR-28-5p mimics up-regulated the expressions of cyclin A, cyclin dependent kinase 2 (CDK2), cyclin D1, and cyclin E but down-regulated the expressions of cleaved caspase-3 and cleaved caspase-9, which was abolished by miR-28-5p inhibitor. Furthermore, luciferase reporter assay verified that miR-28-5p directly targeted MTSS1 3′UTR and down-regulated its expression. MTSS1 overexpression in TE-1 cells inhibited cell proliferation and promoted apoptosis induced by miR-28-5p mimics, whereas silencing of MTSS1 reversed cell progression induced by miR-28-5p inhibitor. We also demonstrated that miR-28-5p could promote esophageal tumor formation in vivo. Hematoxylin–eosin staining, immunohistochemistry, and TUNEL assays confirmed that miR-28-5p antagomir inhibited cell growth and accelerated apoptosis. Our results suggest that miR-28-5p may induce cell proliferation and suppress apoptosis to promote EC tumor formation via decreasing MTSS1 expression. Thus, miR-28-5p may be a potential target for human EC therapy.

Ginsenoside Rh2 attenuates the progression of non-small cell lung cancer by sponging miR-28-5p/STK4 axis and inactivating Wnt/β-catenin signaling

Jun Ma,Di Zhao,Dahai Yu,Wei Song,Xiaofang Yang,Haitao Yin

Background Ginsenoside Rh2 (G-Rh2) exerts anti-tumor activity in non-small cell lung cancer (NSCLC). microRNAs (miRNAs, miRs) play pivotal roles in NSCLC. We aimed to investigate whether G-Rh2 inhibited NSCLC progression by targeting miRNA. Methods Cell viability, apoptosis and cycle were determined by Cell Counting Kit-8, 6-diamidino-2-phenylindole (DAPI) staining and flow cytometry. The potential target miRNAs of G-Rh2 were screened by real-time quantitative polymerase chain reaction (RT-qPCR). The difference in miR-28-5p expression between lung adenocarcinoma (LUAD) tissues and normal tissues or lung squamous cell carcinoma (LUSC) tissues and normal tissues was retrieved from TCGA-LUAD and TCGA-LUSC, respectively. Kaplan–Meier Plotter was conducted to analyze the survival rate for different serine/threonine-protein kinase 4 (STK4) expressions with different prognostic risks. immunohistochemistry of STK4 expression in non-tumor and tumor tissues was analyzed from the HPA database. RT-qPCR and Western blot were adopted for detecting mRNA and protein expression. TargetScan V7.2, miRanda and PITA were adopted for predicting targets of miR-28-5p, overlapped genes were subjected to GO analysis. The interactions of miR-28-5p-Wnt and miR-28-5p-STK4 were detected by TOP/FOP luciferase reporter assay and dual luciferase reporter assay, respectively. Results Current study observed that G-Rh2 reduced miR-28-5p expression in NSCLC cells dose-dependently. miR-28-5p was upregulated in NSCLC tissues and cells. The target genes of miR-28-5p were enriched in negative regulation of Wnt signaling. miR-28-5p inhibitor inactivated Wnt signaling, inhibited cell viability and cell cycle, while enhanced cell apoptosis of NSCLC cells by targeting STK4. G-Rh2 exerted the similar effects with miR-28-5p inhibitor by reducing miR-28-5p. G-Rh2 and miR-28-5p inhibitor exerted a synergistic effect on inhibiting NSCLC tumor growth. Conclusion In conclusion, G-Rh2 attenuates NSCLC development by affecting miR-28-5p/STK4 axis and inactivating Wnt signaling. Taken together, we project out a novel therapeutic target for NSCLC.

Water pollution remediation: synthesis of gray-Tio2/Zno/α-Fe2o3 ternary composite for the photocatalytic degradation of Direct Red 28 dye under solar light

Anjum Muhammad Naveed,Qayyum Muhammad Abdul,Zulfiqar Zunaira,Ahmad Mirza Nadeem,Saif Muhammad Jawwad,Bahadur Ali,Iqbal Shahid,Mahmood Sajid,Alotaibi Khalid M.,Farouk Abd-ElAziem

Based on an appropriate procedure and little toxicity to living things, photocatalytic degradation is noted as one of the best ways to address environmental problems, especially for the effective breakdown of organic pollutants. The present work was aimed at investigating the photocatalytic performance of oxides as ternary nanocomposites in the degradation of Direct Red 28 dye in aqueous solution under solar light. The solvothermal process was employed for the synthesis of Gray-TiO 2 /ZnO/α-Fe 2 O 3 composites. The constructed composite was characterized by means of FTIR, PL, XRD, and SEM, among other methods. The photocatalytic activity of the composite enhances up to 98.82% at pH 3, while it reduces up to 72.21% with an increasing concentration of initial dye at 30 mgL −1 . The synthesized composite had a degradation efficiency of around 99% for Direct Red 28 dye since its recombination rate was high. After the four cyclic runs of the used composite, 85% degradation of Direct Red 28 was observed, which substantiates that the Gray-TiO 2 /ZnO/α-Fe 2 O 3 composite is a stable and efficient catalyst.

Effects of 2-hydroxypropyl-β-cyclodextrin and 28-homobrassinolide on the physiological characteristics, gene expression and terpenoid indole alkaloid accumulation in Catharanthus roseus cambial meristematic cell cultures

Fu Fangfang,Li Haihua,Ling Li,Li Jiahe,Chen Yifei,Hu Weijie,Li Jiayu,Ou Lin,Chen Zhaoxi,Li Jun,Zhou Pengfei

Compared with callus, Catharanthus roseus (CAT) cambial meristematic cells (CMCs) exhibit significantly higher production of pharmacologically active terpenoid indole alkaloids (TIA), such as the anticancer drug vincristine and the antihypertensive drug ajmaline. Elicitors can significantly promote the biosynthesis of secondary metabolites in plant cells. However, the regulation of alkaloid biosynthesis in C. roseus CMCs by elicitors 2-Hydroxypropyl-β-cyclodextrin (H-β-CD) and 28-Homobrassinolide (HBL) is still unknown. Therefore, we established a new cambial meristematic cell line, named C. roseus CMCs-2, and investigated the effects of H-β-CD and HBL individually or in combination on the TIA content of CMCs in Erlenmeyer flasks (100, 250 and 500 mL) or in a 5-L airlift bioreactor. CMCs cultured in the bioreactor and treated with 10.0 g/L H-β-CD and 150 μg/L HBL gave the highest yields of vindoline (4.89 mg/L), catharanthine (2.49 mg/L) vinblastine (3.96 mg/L) and ajmalicine (3.87 mg/L), concentrations that were 241, 277, 165 and 110% higher, respectively, than yields of CMCs cultured in 100-mL flasks without elicitors. These results indicated that CMCs cultured in the bioreactor exhibited enhanced production of vindoline, catharanthine, vinblastine and ajmalicine than those cultured in flasks. Quantitative RT-PCR showed that H-β-CD and HBL up-regulated transcription levels of genes related to the biosynthesis of TIAs. These results imply that H-β-CD and HBL can boost TIA production in CMCs, thereby indicating that C. roseus CMCs have considerable potential as an industrial platform for the production of bioactive TIAs.

1981 Index IEEE Transactions on Nuclear Science Vol. NS-28

This index covers all technical items - papers, correspondence, reviews, etc. - that appeared in this periodical during the year, and items from previous years that were commented upon or corrected in this year. Departments and other items may also be covered if they have been judged to have archival value. The Author Index contains the primary entry for each item, listed under the first author's name. The primary entry includes the coauthors' names, the title of the paper or other item, and its location, specified by the publication abbreviation, year, month, and inclusive pagination. The Subject Index contains entries describing the item under all appropriate subject headings, plus the first author's name, the publication abbreviation, month, and year, and inclusive pages. Note that the item title is found only under the primary entry in the Author Index.

Prognostic value of plasma 7-ketocholesterol in sepsis

Yueyuan Zhang,Sha Xu,Jianxin Xu,Fuchao Xu,Geng Lu,Jiawei Zhou,Shuangshuang Gu,Jun Wang

Background Early evaluation of the severity of sepsis and estimation of its prognosis remains one of the main challenges in current therapeutic strategies. This study aimed to evaluate the prognostic value of plasma 7-ketocholesterol (7-KC) in sepsis. Methods We retrospectively measured by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) the plasma 7-KC concentration in 176 patients with sepsis and 90 healthy volunteers. A multivariate Cox proportional hazard model was introduced to identify independent factors, including plasma 7-KC and clinical features, for the 28-day mortality of sepsis, and a nomogram for predicting the 28-day mortality of sepsis was established. Decision curve analysis (DCA) was performed to assess the prediction model of death risk of sepsis. Results The area under the curve (AUC) of plasma 7-KC in diagnosing sepsis was 0.899 (95% CI = 0.862–0.935, P < 0.001), while it was 0.830 (95% CI = 0.764–0.894, P < 0.001) in diagnosing septic shock. The AUCs of plasma 7-KC in predicting the survival of sepsis patients in the training cohort and the test cohort were 0.770 (95% CI = 0.692–0.848, P < 0.05) and 0.869 (95% CI = 0.763–0.974, P < 0.05), respectively. In addition, high plasma 7-KC expression predicts poor prognosis in sepsis. Then, 7-KC and platelet count were identified as the two factors with significant differences by a multivariate Cox proportional hazard model, and the 28-day mortality probability ranged from 0.002 to 0.985 and was assessed using a nomogram. DCA results revealed that the combination of plasma 7-KC and platelet count showed the best prognostic efficiency of the risk threshold compared to a single factor in both the training cohort and test cohort. Conclusions Collectively, the elevated plasma 7-KC level is an indicator of sepsis and was identified as a prognostic indicator for sepsis patients, providing a landscape for predicting survival in early sepsis with potential clinical utility.

Synthesis of oleanolic acid dimers linked at C-28 and evaluation of anti-tumor activity

Ke-Guang Cheng,Chun-Hua Su,Lin-Dong Yang,Jun Liu,Zhen-Feng Chen

Five dimeric oleanolic acids linked at C-28 by 1,6-hexanediamine, or built around the carbon chains of varying lengths between two carboxyl groups were synthesized, to investigate the effect of internal spacer length and species upon the stereochemical features and anti-tumor activity of the resultant bis-oleanolic acids. The IC 50 values of these dimeric compounds for cytotoxicity evaluation in vitro against Hep-G2, A549, BGC-823, MCF-7 and PC-3 tumor cell lines, were mainly under 10.0 μM. This result was much better than the inhibition of proliferation against tested tumor cell lines of the monomer oleanolic acid and the commercial anticancer drug 5-fluorouracil. The cytotoxicity selectivity detection revealed that dimer 11c exhibited low cytotoxicity towards normal human liver cell HL-7702. A combination of fluorescence staining observation and flow cytometric analysis indicated that 11c could induce Hep-G2 cell apoptosis . Molecular mechanism studies suggested that 11c induced apoptosis is mediated through the intrinsic apoptotic pathway with changes in mitochondrial membrane potential by finally activating effector caspase-3/9 to trigger cell apoptosis. Further studies revealed that 11c caused cell cycle arrest at G1 phase in Hep-G2 cells. Taken together, these results suggest that 11c may be a potential candidate for further cancer research.

A novel fluorescent sensor for highly sensitive detection of ascorbic acid in food based on inhibiting phosphatase-like activity of Zr-based MOF

Zhen Wu,Jinlong Wang,Xing Cheng,Yue Tang,Lian Xia,Xiaojuan Niu,Yuangen Wu

Nanozyme-based sensors for detecting ascorbic acid (AA) generally depend on the reducibility of the analyte. However, these sensors are susceptible to interference from reducing substances in food. Herein, a novel fluorescent sensor for AA detection was developed based on inhibiting the phosphatase-like activity of a Zr-based metal-organic framework (Zr-CAU-28). Hydroxyl-rich AA molecules adsorb on the surface of Zr-CAU-28 through hydrogen bonding with [Zr 6 O 4 (OH) 4 ] cluster, leading to a decrease in the relative content of terminal hydroxyl groups within the catalytic sites. The constructed sensor exhibits a wide detection range (0.08–11 μg·mL −1 ) and low detection limit of 0.025 μg·mL −1 . Potential interfering studies demonstrated the good selectivity of the sensor. Moreover, the fluorescent sensor can effectively detect AA in juices and vitamin C tablets, with the recovery rate ranging from 96.25 % to 108.50 %. This work represents the first application of phosphatase mimics for AA detection, offering a new strategy for food analysis.

Stability of 28 typical prescription drugs in sewer systems and interaction with the biofilm bacterial community

Anchen Liu,Wenting Lin,Ruiliang Ming,Wenqi Guan,Xinying Wang,Ningyi Hu,Yuan Ren

Identifying the attenuation characteristics of drugs in sewage and sewers is one of the important factors to improve the accuracy of wastewater-based epidemiology (WBE) application. In this study, 28 drugs including antidepressants, cardiovascular drugs, antihistamines, anticonvulsants and some of their human metabolites were chosen as the targets to study the hydrolysis , adsorption, and biodegradation at different temperatures in sewage and sewers. The interaction between drugs degradation and community structure of biofilm was also investigated. In the simulated sewers, the removal percentages of 12 parent or drug metabolites are 0–20%, such as demethylvenlafaxine, fluvoxamine, etc., which are highly stable chemicals and suitable to be chosen as biomarkers for WBE back-calculation under appropriate circumstances. Fourteen drugs including venlafaxine and citalopram have removal percentages of 20–60%. While paroxetine and sertraline, with removal percentage of 100%, are the most unstable and cannot be used as biomarkers. Among the 28 drugs, there are 25 drugs that have a higher loss rate in the aerobic sewer than that in the anaerobic sewer in this study. During drug exposure in anaerobic biofilms, species abundance first decreased and then increased. Species abundance and diversity in aerobic biofilm generally showed a decreasing trend. In addition, Proteobacteria and Spirochaetota were the dominant phyla in both sewers.