Scientific Reports
·
2021
·Vol. 11
·期 1
·1-13
·
IF 4.38
Niu Meiyun,Yan Haifeng,Xiong Yuping,Zhang Yueya,Zhang Xinhua,Li Yuan,da Silva Jaime A. Teixeira,Ma Guohua
Sandalwood ( Santalum album L.) is famous for its unique fragrance derived from the essential oil of heartwood, whose major components are santalols. To understand the mechanism underlying the biosynthesis of santalols, in this study, we cloned two related genes involved in the mevalonate pathway in S. album coding for acetyl-CoA C-acetyl transferase (AACT) and 3-hydroxy-3-methyglutary-CoA synthase (HMGS). These genes were characterized and functionally analyzed, and their expression profiles were also assessed. An AACT gene designated as SaAACT (GenBank accession No. MH018694) and a HMGS gene designated as SaHMGS (GenBank accession No. MH018695) were successfully cloned from S. album . The deduced SaAACT and SaHMGS proteins contain 415 and 470 amino acids, and the corresponding size of their open-reading frames is 1538 bp and 1807 bp, respectively. Phylogenetic trees showed that the SaAACT protein had the closest relationship with AACT from Hevea brasiliensis and the SaHMGS proteins had the highest homology with HMGS from Siraitia grosvenorii . Functional complementation of SaAACT and SaHMGS in a mutant yeast strain deficient in these proteins confirmed that SaAACT and SaHMGS cDNA encodes functional SaAACT and SaHMGS that mediate mevalonate biosynthesis in yeast. Tissue-specific expression analysis revealed that both genes were constitutively expressed in all examined tissues (roots, sapwood, heartwood, young leaves, mature leaves and shoots) of S. album , both genes showing highest expression in roots. After S. album seedlings were treated with 100 μM methyl jasmonate, the expression levels of SaAACT and SaHMGS genes increased, suggesting that these genes were responsive to this elicitor. These studies provide insight that would allow further analysis of the role of genes related to the sandalwood mevalonate pathway in the regulation of biosynthesis of sandalwood terpenoids and a deeper understanding of the molecular mechanism of santalol biosynthesis.
研究领域:
植物学
·
遗传学
·
植物生物化学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF RARE EARTHS
·
2012
·
IF 0.901
Xiaoming PENG,Yufeng HU,Chuanwei JIN,Zhixin WANG,Chao MIAO,Zheyu LI
Viscosities were measured for the ternary systems Y(NO 3 ) 3 +La(NO 3 ) 3 +H 2 O, La(NO 3 ) 3 +Ce(NO 3 ) 3 +H 2 O, and La(NO 3 ) 3 +Nd(NO 3 ) 3 + H 2 O and their binary subsystems Y(NO 3 ) 3 +H 2 O, La(NO 3 ) 3 +H 2 O, Ce(NO 3 ) 3 +H 2 O, and Nd(NO 3 ) 3 +H 2 O at 293.15, 298.15 and 308.15 K. The results were used to test the applicability of simple equations for the viscosity of the mixed solutions. The predictions agreed well with measured values, implying that the viscosities of the examined electrolyte solutions could be related to those of their constituent binary solutions using these simple equations.
研究领域:
物理化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
BIOPROCESS AND BIOSYSTEMS ENGINEERING
·
2020
·Vol. 43
·期 5
·831-838
·
IF 2.419
Liu Bin,Wang Xufeng,Zhao Jiahao,Qin Liehao,Shi Lei,Zhou Tao,Liu Shuang,Wang Chunling
Zygosaccharomyces rouxii referred as Yeast S is an important microorganism widely applied in traditional fermented food to accelerate flavor formation. Z. rouxii 3-2 referred as Yeast S 3-2 constructed previously is a new strain having higher salt tolerance than wild type. In this study, salt stress response of synthesis key flavor metabolites 3-methylthiopropanol, 2-phenylethyl alcohol, and isoamyl acetate in Yeast S and S 3-2 were investigated based on SPME–GC–MS and RT-qPCR. Analysis of GC–MS data showed that high salinity led to increase the contents of 3-methylthiopropanol and 2-phenylethyl alcohol both in Yeast S and Yeast S 3-2, while inhibited the synthesis of isoamyl acetate, and the induced effect on Yeast S 3-2 was more pronounced than Yeast S. Furthermore, the RT-qPCR results revealed that the salinity could enhance the genes expression of ARO10 and PDC1 , and stronger effect on Yeast S 3-2 than S. However, the gene expression of AFT1 was reduced in high-salt culture conditions, which was consistent with the results of GC–MS data. The results presented in this study can provide theoretical support of Z. rouxii 3-2 application during food fermentation.
研究领域:
微生物学
·
食品科学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nature Communications
·
2021
·Vol. 12
·期 1
·1-7
·
IF 14.919
Liu Yanting,Wang Ran,Qi Haifeng,Liu Xiao Yan,Li Guangyi,Wang Aiqin,Wang Xiaodong,Cong Yu,Zhang Tao,Li Ning
The exploration of highly efficient processes to convert renewable biomass to fuels and value-added chemicals is stimulated by the energy and environment problems. Herein, we describe an innovative route for the production of methylcyclopentadiene (MCPD) with cellulose, involving the transformation of cellulose into 3-methylcyclopent-2-enone (MCP) and subsequent selective hydrodeoxygenation to MCPD over a zinc-molybdenum oxide catalyst. The excellent performance of the zinc-molybdenum oxide catalyst is attributed to the formation of ZnMoO 3 species during the reduction of ZnMoO 4 . Experiments reveal that preferential interaction of ZnMoO 3 sites with the C=O bond instead of C=C bond in vapor-phase hydrodeoxygenation of MCP leads to highly selective formations of MCPD (with a carbon yield of 70%).
研究领域:
能源与环境科学
·
催化化学
✓ 已验证
DOI
高影响因子
APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING
·
2021
·Vol. 127
·期 2
·1-11
·
IF 2.584
Hu Shuting,He Junfeng,Chen Fuming,Liu Bin,zhai Wangjian,He Qinyu
Constructing Z-scheme heterojunction photocatalyst with strong redox ability to make for enhanced photocatalytic performance and efficient charge separation is extremely attractive but still underdeveloped. Herein, a Z-scheme heterojunction structured La(OH) 3 @In 2 S 3 composite (labeled by “LIS”) with photocatalytic for the methylene orange (MO) degradation under simulated light irradiation has been developed. The as-prepared LIS, together with commercial La(OH) 3 and pure In 2 S 3 fabricated with the identical processing method and starting materials as those of LIS, was characterized by X-ray diffraction, UV–vis diffuse reflectance spectra, scanning electron microscopy, high-resolution transmission electron microscopy, X-ray photoelectron spectroscopy, photoluminescence spectra and electrochemical impedance spectroscopy. The results show the heterojunction of La(OH) 3 /In 2 S 3 has prolonged the lifetime of the photo-generated carriers. The photocatalytic activity test shows that over only a small amount (0.02 g in 100 ml MO) of LIS, the photodegradation rate of 95% toward MO can be obtained in 90 min, which is about 3.4 times higher than that over pure In 2 S 3 . The active species trapping experiments indicate that there were four active species playing roles in photodegradation as the following order: e – = ∙OH < h + < ∙O 2 – . A mechanism of Z-scheme heterojunction was proposed and well explained the enhanced photocatalytic performance. This work provides a new cost-effective photocatalyst with high photocatalytic properties.
研究领域:
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ORGANIC CHEMISTRY
·
2022
·
IF 4.198
Xiang Liu,Yuhan Wang,Huitao Zheng,Junyan Huang,Zhengquan Su,Limin Zhao,Hua Cao
Here, we report a facile and metal-free method for the construction of dihydrooxazine derivatives via a formal (3 + 3) annulation reaction of naphthols and 1,3,5-triazinanes. The 1,3,5-triazinanes were utilized as a formal three-atom synthon (C–N–C) for cycloaddition. In addition, dihydrothiazine and tetrahydrobenzoquinazoline derivatives could also be produced in good yields by this strategy under catalyst-free and additive-free conditions.
研究领域:
有机化学
·
合成化学
✓ 已验证
DOI
PubMed
高影响因子
ACTA PHARMACOLOGICA SINICA
·
2024
·
IF 6.9
Su Qi,Wang Jing-jing,Ren Jia-yan,Wu Qing,Chen Kun,Tu Kai-hui,Zhang Yu,Leong Sze Wei,Sarwar Ammar,Han Xu,Zhang Mi,Dai Wei-feng,Zhang Yan-min
Parkin ( PARK2 ) deficiency is frequently observed in various cancers and potentially promotes tumor progression. Here, we showed that Parkin expression is downregulated in liver cancer tissues, which correlates with poor patient survival. Parkin deficiency in liver cancer cells promotes migration and metastasis as well as changes in EMT and metastasis markers. A negative correlation exists between TMEFF1 and Parkin expression in liver cancer cells and tumor tissues. Parkin deficiency leads to upregulation of TMEFF1 which promotes migration and metastasis. TMEFF1 transcription is activated by Parkin-induced endogenous TGF-β production and subsequent phosphorylation of Smad2/3 and its binding to TMEFF1 promotor. TGF-β inhibitor and TMEFF1 knockdown can reverse shParkin-induced cell migration and changes of EMT markers. Parkin interacts with and promotes the ubiquitin-dependent degradation of HIF-1α/HIF-1β and p53, which accounts for the suppression of TGF-β production. Our data have revealed that Parkin deficiency in cancer leads to the activation of the TGF-β/Smad2/3 pathway, resulting in the expression of TMEFF1 which promotes cell migration, EMT, and metastasis in liver cancer cells.
研究领域:
肿瘤学
·
分子生物学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Dai-Xu Wei,Duanfang Cai,Youguo Tan,Kezhi Liu,Jin-Wei Dao,Xiang Li,Aikeremujiang Muheremu
Huperzine A (HupA) is used in Alzheimer's disease (AD) therapy for its effective inhibition of acetylcholinesterase (AChE) and enhancement of cholinergic neuronal function. However, direct oral administration and injection of HupA cause side effects like nausea, anorexia, and rapid metabolism. Using a tripolymer, poly(3-hydroxybutyrate- co -3-hydroxyvalerate-co-3-hydroxyhexanoate (PBVHx), from the polyhydroxyalkanoate (PHA) family synthesized via synthetic biology, we present a novel AD therapy strategy with peritoneally administered PBVHx microspheres loaded with HupA (HupA-PBVHxMs). This approach extends HupA's metabolic duration in the blood and brain, enhancing AChE inhibition efficacy. Uniformly sized HupA-PBVHxMs, created using microfluidics and rotary evaporation, show up to 70.4 % drug encapsulation efficiency, sustained HupA release for 40 days, reduced neurotoxicity from Aβ25–35, and maintained in vivo HupA supply and AChE inhibition for over 20 days. In cognitive tests, HupA-PBVHxMs improved function in AD mice. Thus, PBVHx microspheres with slower HupA release and lower biotoxicity offer a superior platform for sustained AChE inhibitor release, outperforming commercial PLGA microspheres.
研究领域:
药学
·
神经科学
·
生物技术
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2019
·Vol. 55
·期 4
·1531-1538
·
IF 2.281
Yangping Ding,Bingcan Chen,Huayi Suo,Huarong Tong
Summary Theaflavin-3,3′-digallate (TFDG) is the main product formed by oxidative condensation between (−)-Epigallocatechin-3-gallate (EGCG) and (−)-Epicatechin-3-gallate (ECG). Some sub-products, such as homo- and hetero-oligomers of EGCG and ECG, are also produced simultaneously, which significantly decreases the yield of TFDG. In general, the optimum temperature for TFDG synthesis is 20 °C below which can reduce its generation resulting in the coexistence of a large amount of unreacted substrates (ECG and EGCG). Therefore, a higher possibility of increasing the formation of TFDG could be achieved if a suitable synthetic method can be developed at a low temperature. Our results showed that simultaneous dropwise addition of both EGCG and ECG could markedly enhance the content of TFDG at ice bath. Using such technique, the production of TFDG increased by 47.60% as compared to the group synthesised under the optimum temperature. The absorbance of TFDG at 278 nm is 2.36 times higher than that of ECG with equimolar concentration. Therefore, a rapid, simple and accurate calculation method of TFDG yield was developed via the spectral correlation. Based on the counting method, the yield of TFDG was 30.58% by using the novel synthetic method of TFDG.
研究领域:
食品科学
·
植物化学
✓ 已验证
DOI
高影响因子
JOURNAL OF CHEMICAL THERMODYNAMICS
·
2020
·
IF 2.888
Jinyi Chen,Hongxing Wang
Density and viscosity data of pure 3-chloro-4-fluoronitrobenzene and 3-chloro-2-fluoronitrobenzene were obtained in temperature range from 318.15 K to 348.15 K at the local atmospheric pressure of 99.7 kPa. The value of corresponding saturated vapour pressure of two isomers were determined in the temperature range of 409 K to 517 K. The relationship between temperature and density can be well correlated by the linear equation. The viscosity data was well described by the Litovitz, Ghatee, VFT, and Andrade equations, and the calculated value by the VFT equation is consistent with the measured data. Both the Antoine and Riedel equations can accurately describe the relationship between saturated vapour pressure and temperature of 3-chloro-4-fluoronitrobenzene and 3-chloro-2-fluoronitrobenzene, which can meet the requirements for chemical design. In addition, the thermal expansion coefficient was determined based on the density data, and the molar vaporisation enthalpy of two isomers was evaluated using the Clausius-Clapeyron equation.
研究领域:
化学工程
·
物理化学
✓ 已验证
DOI
高影响因子
CURRENT MICROBIOLOGY
·
2022
·Vol. 79
·期 12
·1-10
·
IF 2.343
Lu Min,Liu Bingxin,Xiong Wenbin,Liu Xinchun
The novel phage ST-3, capable of infecting the foodborne pathogen Salmonella Typhimurium, was isolated from wastewater. The Biological characters and genome information of ST-3 were analyzed. In the in vitro assay, the phage ST-3 with a MOI of 10 effectively inhibited the growth of Salmonella Typhimurium CGMCC 1.1174 in 6 h. The inhibitory effect of combination phage ST-3 and antibiotics was also studied, the removal rate of planktonic host exposed to ST-3 and levofloxacin hydrochloride at the same time, or to ciprofloxacin followed by ST-3, is higher than that exposed to antibiotic dosing group alone and antibiotic + phage dosing group. The phage ST-3 combined with 0.5 µg/mL levofloxacin hydrochloride resulted in the largest decrease in biofilm biomass at 54%. The phage ST-3 could be a potential agent to control Salmonella Typhimurium growth and provide instruction for use it and antibiotics together.
研究领域:
微生物学
·
药理学
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
IONICS
·
2015
·Vol. 21
·期 9
·2503-2510
·
IF 1.754
Ma Xueli,Wang Chunfeng,Ren Hao,Chen Yujuan,Zhuo Kelei
1-Hexyl-3-methylimidazolium tetraphenylborate ([C 6 mim]-TPB) was synthesized and explored as ion exchanger in carbon paste electrode (CPE) as an ion-selective electrode (ISE). The electrode was found to be optimal with the composition of 42 % graphite powder, 20 % paraffin oil, 30 % ion exchanger, 5 % MWCNTs, and 3 % nano-silica. The as-prepared electrode exhibits a Nernstian response (59.2 mV per decade) to 1-hexyl-3-methylimidazolium cation in the concentration range from 1.0 × 10 −5 to 1.0 × 10 −2 mol kg −1 . The novel as-prepared MWCNTs/nano-silica/CPE was successfully applied in the detection of [C 6 mim] + in distilled water, tap water, and river water with satisfactory results.
研究领域:
分析化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）