INSTRUMENTATION SCIENCE & TECHNOLOGY
·
2017
·
IF 0.874
Shuyan Niu,Yajie Fang,Kun Zhang,Juan Sun,Jun Wan
A facile and sensitive fluorescent strategy for the determination of dopamine is reported using 2, 3-diaminophenazine. Dopamine caused autooxidation in the presence of oxygen with catalysis by Mn2+ and formed 2, 3-dihydro-1H-indole-5,6-dione. 2, 3-Diaminophenazine provided relatively strong fluorescence. 2, 3-Diaminophenazine in aqueous solution caused fluorescence quenching when the aminochrome condensed with 2, 3-diaminophenazine to allow the determination of dopamine. Under the optimized conditions, the linear dynamic range for dopamine was from 2.0 × 10−6 mol · L−1 to 6.1 × 10−5 mol · L−1 with a detection limit of 1.76 × 10−6 mol · L−1.
研究领域:
分析化学
·
生物医学
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Bio Materials
·
2020
·
IF 0
Pengjuan Ni,Siyuan Liu,Yuanyuan Jiang,Chuanxia Chen,Bo Wang,Chenghui Zhang,Jianbin Chen,Yizhong Lu
We report a fluorescence assay for alkaline phosphatase (ALP) detection using in situ generation of 2,3-diaminophenazine (OPDox) through an inner filter effect (IFE). AgNO3 can oxidize o-phenylenediamine in a short time to obtain fluorescent OPDox. p-nitrophenol is obtained from ALP-catalyzed hydrolysis of p-nitrophenylphosphate, which can result in the fluorescence quenching of OPDox via the IFE. Consequently, in situ generation of OPDox can be utilized for evaluating the activity of ALP. The fluorescence of OPDox decreases linearly with increasing concentration of ALP in the range from 0.1 to 8.0 mU mL–1, and the detection limit is calculated to be 0.05 mU mL–1. More importantly, this assay has been used to construct a logic gate. The IFE-based fluorescence assay exhibits several distinctive advantages, including high sensitivity, good selectivity, and simple operation. Consequently, it may pave the way for the detection of ALP by utilization of the in situ generation of fluorophores.
研究领域:
生物化学
·
医学诊断
✓ 已验证
产品关联
DOI
PubMed
ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Feng Zhang,Mengke Wang,Lili Zhang,Xingguang Su
Herein, we developed a novel and sensitive ratiometric fluorescence strategy for pH sensing and urea detection based on MoS 2 quantum dots (MQDs) and 2, 3-diaminophenazine (DAP). The DAP was pH-sensitive and can be used as pH indicator while MQDs was pH-insensitive and acted as a reference fluorescent signal. The fluorescence intensity of DAP at 568 nm exhibited increases gradually while the fluorescence intensity of MQDs at 420 nm kept unchanged as the pH changed from 3.0 to 9.0. So the ratio of the two fluorescence intensities at 568 nm and 420 nm can be used to monitor the change of pH. The pH-sensing system showed a linear and reversible respond to pH in the range of 3.8–6.0 with an interval of 0.2 pH unit. Furthermore, the enzymatic reaction of urea in the presence of urease can lead to the pH increase of the solution, which can be used to detect urea by using the above-mentioned pH-sensing system. There exhibited a good linear relationship between the ratiometric fluorescence intensity of I 568 /I 420 and urea concentrations ranging from 5 to 700 μM. And a low detection limit of 1.8 μM was obtained. The proposed strategy exhibited excellent selectivity and sensitivity. Additionally, the developed ratiometric fluorescence strategy was successfully applied to detect urea in water samples.
研究领域:
生物化学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2021
·
IF 8.128
Jiandong Liang,Mengyao Tang,Liwei Cheng,Qiaonan Zhu,Runa Ji,Xiao Liu,Qi Zhang,Hua Wang,Zhitian Liu
Organic materials are attracting extensive attention as promising cathodes for rechargeable aqueous zinc-ion batteries (ZIBs). However, most of them fail to implement the requirement of batteries with combined high-rate and long-cycle performance. Herein, we report a flexible organic molecule 2,3-diaminophenazine (DAP) which exhibits ultrahigh rate performance up to 500C and high capacity retention of 80% after 10,000 cycles at 100C (25.5 A g −1 ). Moreover, the Zn 2+ storage mechanism in the DAP electrode is revealed by ex-situ characterization technologies and theoretical calculation, and the redox active centers C N participate in the reversible electrochemical reaction process. Furthermore, electrochemical analyses show that surface-controlled electrochemical behavior contributes to the high-rate performance of DAP cathodes. Besides, its excellent long-cycle performance can be ascribed to the suppressed DAP dissolubility by using a modified glass fiber separator with carbon nanotubes (CNT) film. Our work provides useful insight into the design of high-rate and long-life ZIBs .
研究领域:
材料科学
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电化学
·
储能技术
✓ 已验证
DOI
高影响因子
ANALYST
·
2024
·
IF 3.6
Chengchen Tan,Xiaolong Yan,Xingchang Lu,Jianxiu Wang,Xinyao Yi
Breast cancer, as a malignant tumor with the highest incidence in the world, poses a great threat to human life and health. Early diagnosis of breast cancer may help enhancing the survival rate. In this work, a colorimetric and fluorescent dual-mode biosensor based on CRISPR-Cas12a system was constructed for the detection of breast cancer biomarker BRCA1. The intact G4 DNA could form DNAzyme in the assistance of K+ and hemin, catalyzed the oxidation of o-phenylenediamine (OPD) under the assistance of hydrogen peroxide (H2O2), and generate the oxidation product 2,3-diaminophenazine (DAP) with obvious absorption and fluorescence peaks. The presence of the target BRCA1 can activate the trans-cleavage activity of CRISPR-Cas12a, cleaving the G4 DNA and inhibiting the catalytic oxidation of OPD. The target BRCA1 was quantitatively determined by detecting both the absorbance and fluorescence intensity of DAP. Compared with single signal analysis, dual-signal output mode provided more reliable detection results. The detection limits were calculated to be 0.615 nM for colorimetric method and 0.289 nM for fluorescence method. The dual-mode biosensor showed good selectivity for BRCA1 and can resist the interference of complex substrates, which has great potential in biomedical detection.
研究领域:
生物医学工程
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分子生物学
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癌症研究
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生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2021
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IF 4.098
Xiaoyan Sun,Yuxin Guo,Ruizhi Wen,Hongchang Li
Cu 2+ is a major environmental pollutant. An efficient measurement for Cu 2+ is urgently needed. In this report, we have developed a new sensitive and selective ratiometric sensing platform using 7-amino-4-methylcoumarin (AMC) for detecting Cu 2+ in real samples. In the presence of Cu 2+ , o-phenylenediamine (OPD) could be catalytically oxidized to 2,3-diaminophenazine (DAP), which could react with AMC, leading to quench the fluorescence intensity of AMC at 438 nm. Meanwhile, DAP provided a new emission peak at 557 nm. Based on the efficient overlapped spectrum of AMC and DAP, a ratiometric sensing platform through fluorescence resonance energy transfer (FRET) was carried out. Furthermore, the as-proposed strategy displayed the linear relationship in the wide range from 6 to 250 μM with a low detection limit of 0.059 μM, and the recoveries of the spiked samples in real samples ranged from 86.5% to 110.1%. Moreover, comparing the visual fluorescence colors of the real samples with the standard colorimetric card, we used the as-proposed strategy as a solid-based platform for realizing an efficient semi-quantitative detection of Cu 2+ via naked-eye visual fluorescence mode without any complicated instrument and operation. The above results implied that the as-proposed strategy could be used in the practice determination of Cu 2+ .
研究领域:
环境科学
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分析化学
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光谱学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2022
·Vol. 414
·期 4
·1651-1662
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IF 4.478
Zhang Wei,Zhang Yu,Liu Xin,Zhang Yue,Liu Yibing,Wang Wei,Su Rui,Sun Ying,Huang Yibing,Song Daqian,Wu Yanhua,Wang Xinghua
A sensing platform with both ratiometric fluorescence and colorimetric responses towards copper(II) ions (Cu 2+ ) and D-penicillamine (D-pen) was constructed based on carbon dots (CDs). o -Phenylenediamine (OPD) was employed as a chromogenic development reagent for reaction with Cu 2+ to generate the oxidation product 2,3-diaminophenazine (oxOPD), which not only emits green fluorescence at 555 nm, but also quenches the blue fluorescence of CDs at 443 nm via the inner filter effect (IFE) and Förster resonance energy transfer (FRET). Additionally, oxOPD exhibits obvious absorption at 420 nm. Since the intense chelation affinity of D-pen to Cu 2+ greatly inhibits the oxidation of OPD, the intensity ratio of fluorescence at 443 nm to that at 555 nm ( F 443 / F 555 ) and the absorbance at 420 nm ( A 420 ) were conveniently employed as spectral response signals to represent the amount of D-pen introduced into the testing system. This dual-signal sensing platform exhibits excellent selectivity and sensitivity towards both Cu 2+ and D-pen, with low detection limits of 0.019 μM and 0.092 μM, respectively. In addition, the low cytotoxicity of the testing reagents involved in the proposed sensing platform facilitates its application for live cell imaging. Graphical abstract
研究领域:
分析化学
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材料科学
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生物化学
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纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biosensors-Basel
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2022
·Vol. 12
·期 10
·830
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IF 5.743
Juanjuan Huang,Yiyun Yao,Yanling Chen,Tianran Lin,Li Hou,Dianping Tang
Copper peroxide/zeolitic imidazolate framework/polydopamine nanoparticles (CP/ZIF-8/PDA)-based fluorescence-linked immunosorbent assay (FLISA) was designed for the sensitive and high-throughput determination of carcinoembryonic antigen (CEA) by self-supplied H2O2generation. Specifically, the CEA aptamer was modified on the surface of CP/ZIF-8/PDA to form an immunoprobe. The structures of CP and ZIF-8 could be broken under acidic conditions, and produced the Cu2+and H2O2due to the dissociation the CP. A subsequent Fenton-type reaction of Cu2+and H2O2generated hydroxyl radical (·OH). o-phenylenediamine (OPD) was oxidized by the ·OH to form 2, 3-diaminophenazine (DPA) with a significant fluorescence signal. CP/ZIF-8/PDA could be used as an efficient Fenton-type reactant to generate a large amount of ·OH to promote OPD oxidation. The sensitive detection of CEA could be realized. Under optimal conditions, the FLISA platform displayed a linear detection range from 0.01 to 20 ng mL−1with a detection limit of 7.6 pg mL−1for CEA. This strategy has great application potential for sensitive and high-throughput determination for other biomarkers in the field of biomedicine.Keywords:fluorescence-linked immunosorbent assay;Fenton-type reaction;carcinoembryonic antigen
研究领域:
生物医学工程
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分析化学
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生物传感器
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癌症诊断
✓ 已验证
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 7
·1-13
·
IF 5.3
Li Ziqiang,Liang Shuang,Zhang Changsheng,Zhou Li,Luo Fengjian,Lou Zhengyun,Chen Zongmao,Zhang Xinzhong,Yang Mei
A ratiometric fluorescence sensing strategy has been developed for the determination of Cu 2+ and glyphosate with high sensitivity and specificity based on OPD (o-phenylenediamine) and glutathione-stabilized gold nanoclusters (GSH-AuNCs). Water-soluble 1.75-nm size GSH-AuNCs with strong red fluorescence and maximum emission wavelength at 682 nm were synthesized using GSH as the template. OPD was oxidized by Cu 2+ , which produced the bright yellow fluorescence oxidation product 2,3-diaminophenazine (DAP) with a maximum fluorescence emission peak at 570 nm. When glyphosate existed in the system, the chelation between glyphosate and Cu 2+ hindered the formation of DAP and reduced the fluorescence intensity of the system at the wavelength of 570 nm. Meanwhile, the fluorescence intensity at the wavelength of 682 nm remained basically stable. It exhibited a good linear relationship towards Cu 2+ and glyphosate in water in the range 1.0–10 µM and 0.050–3.0 µg/mL with a detection limit of 0.547 µM and 0.0028 µg/mL, respectively. The method was also used for the semi-quantitative determination of Cu 2+ and glyphosate in water by fluorescence color changes visually detected by the naked eyes in the range 1.0–10 µM and 0.30–3.0 µg/mL, respectively. The sensing strategy showed higher sensitivity, more obvious color changes, and better disturbance performance, satisfying with the detection demands of Cu 2+ and glyphosate in environmental water samples. The study provides a reliable detection strategy in the environment safety fields. Graphical abstract
研究领域:
分析化学
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环境科学
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纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2025
·
IF 6.1
Lin Chai,Haoyu Chen,Xing Yang,Jing Liu,Qiquan Yang,Shu Huang,Min Tang,Xiaohua Zhu,Haitao Li,Youyu Zhang,Meiling Liu
Ratiometric fluorescent nanoplatforms with self-calibrating capabilities offer enhanced detection accuracy by minimizing environmental interference. However, challenges such as signal cross-interference and nanomaterial instability persist in complex biological systems. To address these issues, we developed a glutathione (GSH)-responsive ratiometric fluorescent nanoprobe by integrating a porphyrin-based metal-organic framework (PCN-224) with MnO 2 nanosheets (NSs). The PCN-224 serves as both a nanocarrier and luminescent moiety, while MnO 2 acts as oxidase (OXD) mimetic nanozyme and the reactive site for GSH. The ratiometric detection mechanism relies on the intrinsic fluorescence of PCN-224 (emission at 655 nm) and the OXD-like activity of PCN-224@MnO 2 , which catalyzes the oxidation of o -phenylenediamine (OPD) to generate fluorescent 2,3-diaminophenazine (DAP, emission at 570 nm). Upon GSH exposure, the redox reaction between GSH and MnO 2 produces Mn 2+ , quenching the OXD-like activity and diminishing the DAP fluorescence, while the PCN-224 fluorescence recovers due to MnO 2 decomposition. The nanoplatform exhibits a wide detection range spanning from 0.1 to 60 μM with a low detection limit of 15 nM and high selectivity. Furthermore, it enables accurate GSH quantification in complex biological matrices, including human serum and cell lysates, and facilitates real-time monitoring of endogenous and exogenous GSH in living cells via fluorescence imaging. This study not only presents sensitive and reliable platform for GSH detection, but also overcomes key limitations in ratiometric sensing, such as signal interference and nanomaterial instability, by utilizing a stimuli-responsive fluorescent nanoprobe design.
研究领域:
分析化学
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纳米技术
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生物化学
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生物传感器
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荧光成像
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Wen Jie Zhang,Shi Gang Liu,Lei Han,Hong Qun Luo,Nian Bing Li
A simple ratiometric fluorescent and colorimetric dual-signal sensing system for Cu 2+ and pyrophosphate ion (PPi) detection is established based on N-doped carbon dots (N-CDs) which are synthesized via one-step hydrothermal approach. In the strategy, 2,3-diaminophenazine (oxOPD), the oxidation product of o-phenylenediamine (OPD), can be adsorbed on the surface of N-CDs through electrostatic interaction, which efficiently quenches the fluorescence of N-CDs, meanwhile, oxOPD provides a new emission peak at 553 nm. In virtue of the selective oxidative and chromogenic reaction of OPD with Cu 2+ , a dual-readout sensing system for Cu 2+ is achieved. In addition, the redox and chromogenic reaction among them can be inhibited by PPi, which protects effectively the fluorescence of N-CDs from quenching. This sensing system exhibits good selectivity and sensitivity toward Cu 2+ and PPi over other analytes with a low detection limit of 23 nM and 0.7 μM, respectively. Furthermore, the proposed sensing system displays a prospective application for quantitative assay of Cu 2+ and PPi in practical samples.
研究领域:
分析化学
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材料科学
·
传感器与执行器
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 11
·1-11
·
IF 5.3
Xu Yuanyuan,Yin Qingqing,Du Ningjing,Yi Yinhui,Zhu Gangbing
By preparing Cu@CuO aerogel as a nanozyme which exhibits prominent peroxidase-like (POD) activity, an innovative homogeneous electrochemistry (HEC) coupled with the colorimetry dual-model sensing strategy is proposed to detect perfluorooctane sulfonate (PFOS) for the first time. Cu@CuO aerogel accelerates the oxidation process of colorless o-phenylenediamine to form yellow 2,3-diaminophenazinc (DAP), and meanwhile, DAP as an electroactive substance creates a reduction peak current upon the electrochemical measurements. Interestingly, in the presence of PFOS, the POD activity of Cu@CuO aerogel is inhibited since the specific coordination between PFOS and Cu(II) can cover the active sites, resulting in the color of the sensing system becoming light and the peak current of DAP decreasing. This innovative dual-mode detection method showed excellent electrochemical detection of PFOS in the concentration range 10.0 ~ 1125.0 nM with a limit of detection (LOD) as low as 3.3 nM and a LOD of 20.8 nM in the colorimetric detection in the range 62.3 ~ 875 nM. Furthermore, the sensor was successfully used for the analysis of real samples with an RSD value ≤ 6.5%. The successful application of this two-mode sensing method for the determination of PFOS holds promise for the detection of other contaminants in the future. Graphical Abstract
研究领域:
分析化学
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环境科学
·
纳米技术
·
传感器
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）