MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 3
·131
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IF 5.4
Jin Meng,Ning Qiong,Zhang Mengping,Wei Haiyan,Meng Xiao,Chen Wenwen,Pan Hui,Xiu Haidi,Liu Lisheng,Wang Cuijuan
A novel surface-enhanced Raman scattering (SERS) based strategy is described for the rapid identification and quantitative determination of deltamethrin and its metabolite 3-phenoxybenzaldehyde in biological samples. A multifunctional sensor was developed using metal-organic framework-801 (MOF-801) and silver nanoflowers (Ag NFs), synthesizing an Ag NFs/MOF-801 composite. This composite is a dual-function material, acting as both a SERS substrate and an adsorbent, which was integrated into a portable 10-well plate. The addition of MOF-801 enhances enrichment selectivity and efficiency, while the abundant tips and rough surfaces of Ag NFs significantly improve SERS sensitivity. The determination of deltamethrin revealed a marked linear relationship between the SERS signal intensity and the logarithmic analyte concentration, with R-values falling within a range 0.959 to 0.972, with detection limits between 0.07 and 0.15 ppb, indicating suitability for clinical applications. This method successfully analyzed a patient sample, showing strong agreement with conventional chromatographic results while ensuring both practicality and accuracy. Notably, the detection process does not necessitate any sample preparation and can be completed within five min, highlighting its potential as an efficient and user-friendly POCD sensor for diagnosing poisoning in emergency settings.
Section
Graphical
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MICROCHEMICAL JOURNAL
·
2020
·
IF 3.594
Yongna Zhao,Dandan Du,Qiannan Li,Wenjie Chen,Qingchen Li,Qingzhu Zhang,Ning Liang
A highly binding dummy template molecularly imprinted polymers (Fe 3 O 4 -NH 2 @GO@MIPs) was synthesized used Fe 3 O 4 -NH 2 @GO as carrier by dummy surface molecular imprinting technique (DSMIT) for the first time. Fe 3 O 4 -NH 2 @GO@MIPs were characterized by fourier transform infrared (FT-IR), scanning electron microscope (SEM), transmission electron microscope (TEM), thermogravimetric analysis (TGA), brunauer emmett teller (BET) and vibrating specimen magnetometer (VSM). Adsorption and selectivity experiments of Fe 3 O 4 -NH 2 @GO@MIPs and non-imprinted polymers (Fe 3 O 4 -NH 2 @GO@NIPs) verified that the Fe 3 O 4 -NH 2 @GO@MIPs had a better selectivity and adsorption properties for five pyrethroids pesticides. Adsorption test results revealed that the adsorption behaviors of pyrethroids onto Fe 3 O 4 -NH 2 @GO@MIPs followed Langmuir equation adsorption isotherm and pseudo-second-order kinetic models. Fe 3 O 4 -NH 2 @GO@MIPs were used as sorbent in magnetic solid phase extraction (MSPE) to extract five pyrethroids from fruit juice samples, following desorption with acidified ethyl acetate, the pyrethroids were quantified by gas chromatography with electron capture detection (GC-ECD). The developed method MSPE coupled with GC-ECD achieved satisfactory recovery (72.1–106.8%) with RSD less than 10.8%, and the detection limits (LOD) for fenpropathrin (FENP), lambda-cyhalothrin (LC), cyfluthrin (CYF), fenvalerate (FEN) and deltamethrin (DEL) ranged from 0.007 to 0.015 μg/L. Compared to other methods, the procedure MSPE-GC-ECD based on Fe 3 O 4 -NH 2 @GO@MIPs in this work demonstrated to be a more convenient and practical method for the separation, enrichment, and detection of pyrethroids pesticides from fruit juice samples.
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FOOD AND CHEMICAL TOXICOLOGY
·
2023
·
IF 4.3
Yiwen Cui,Yingying Sun,Hang Yu,Yahui Guo,Weirong Yao,Yunfei Xie,Fangwei Yang
Pyrethroid insecticides (PIs), a class of structurally similar non-persistent organic pollutants, can be degraded and metabolized to more toxic, and longer half-life products. In this study, the binding interaction mechanisms between human serum albumin (HSA) and the main degradation metabolites of PIs, 3-phenoxybenzoic acid (3-PBA) and 4-fluoro-3-phenoxybenzoic acid (4-F-3-PBA), were studied by theoretical simulation and experimental verification. Steady state fluorescence spectra showed that the fluorescence quenching mechanism was static. According to the binding constant, 4-F-3-PBA (1.53 × 10 5 L mol −1 ) was bound more strongly to HSA than 3-PBA (1.42 × 10 5 L mol −1 ) in subdomain ⅡA (site I). It was found by isothermal titration calorimetry that the metabolites and HSA spontaneously combined mainly through hydrogen bond and van der Waals interaction . Ultraviolet absorption spectra and circular dichroism spectra showed that the metabolites caused slight changes in the microenvironment and conformation of HSA. The above results were proved by molecular docking. The toxicity properties of the metabolites were further analyzed by software, and 4-F-3-PBA was found to be more toxic than 3-PBA. Considering the high exposure level of these metabolites in food , the environment and human body, it is necessary to further explore the toxicity of PIs metabolites.
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Soft Matter
·
2024
·
IF 2.9
Keke Yang,Hou Bo,Dewei Ma,Mingwei Peng,Qinglong Liu,Ziwen Heng,Zhongwei Gu,Xuhan Liu,Siyuan Chen
Phenylboronic acid (PBA) is a widely exploited glucose-sensitive element for constructing glucose-responsive hydrogels to enable smart insulin delivery. However, its relatively high intrinsic pKa affects its binding with glucose under physiological conditions and thus limits its application. Herein, we developed a series of boronate-containing PLP–PBA polymers by conjugating glucose-sensitive 3-aminophenylboronic acid (3-PBA) onto the backbone of a metabolite-derived, pH-responsive poly-L-lysine isophthalamide (PLP) polymer with a pKa value of 4.4 at various substitution degrees. Dual-responsive LME–(PLP–PBA) hydrogels were further synthesized by crosslinking the PLP–PBA polymers with L-lysine methyl ester (LME). The rheological properties and swelling ratio of the hydrogel could be manipulated by the PBA grafting degree and crosslinking ratio. With the increase of pH and glucose concentration, the pore size of the hydrogel enhanced, thus promoting the release of loaded insulin. Under physiological conditions, the hydrogel with optimal formulation could establish acute pH-responsive and glucose-responsive insulin release. The development of this dual-responsive hydrogel suggests a strategy to overcome the high pKa problem associated with PBA and provide a promising delivery system for smart insulin delivery.
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Food Bioscience
·
2025
·
IF 5.9
Li Ming,Yongfei Zhou,Ning Li,Xinhua Zhou,Hongyan Zhang,Xiaobo Zou,Can Zhang
The integration of nanobody and nanozyme has emerged as a transformative strategy in immunoassays, offering enhanced sensitivity, stability, and versatility compared to conventional antibody-enzyme conjugates. Herein, we successfully developed a ternary nanostructure by integrating silica nanoparticles (SNPs), polydopamine (PDA), and platinum nanoparticles (Pt NPs), yielding SNPs@PDA@Pt (SPP) with robust nanozyme catalytic activity. Notably, the SPP was surface-modified with polyethyleneimine (PEI) to generate positively charged PEI-modified SNPs@PDA@Pt (PSPP). This modification substantially improved the coupling efficiency with anti-3-phenoxybenzoic acid nanobodies (3-PBA Nbs) via electrostatic interactions. The PSPP-based coupling achieved a remarkable efficiency of 92.28 %, representing about a 4.5-fold increase over the SPP method (20.55 %). The PSPP@3-PBA Nbs nanocomposite was successfully integrated into both ELISA and LFIA platforms, achieving remarkable detection limits (0.001 ng/mL for ELISA and 0.003 ng/mL for LFIA). The nanocomposite also showed excellent stability, with no significant loss of immunobinding capacity or catalytic activity over 30 days. This work presents a novel design strategy for constructing high-performance nanozyme-nanobody nanocomposites, which shows great promise for ultra-sensitive 3-PBA detection in resource-limited settings.
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ACS Applied Nano Materials
·
2024
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IF 5.3
Sifang Zhao,Fengjiao Chen,Xianwu Chen,Hongze Liang,Hui Tan,Lingling Zhao
The tumor microenvironment is characterized by several hallmarks such as an acidic pH and high glucose levels in tumor tissues and increased expression of specific proteins and/or sugars on the surface of tumor cells. These unique hallmarks of tumors can be considered in the design of multifunctional drug delivery nanosystems to improve the efficiency of tumor therapy through targeted drug delivery and specific drug release in the tumor tissue. In this study, phenylboronic acid-modified pH- and glucose-responsive polymer micelles were designed for the targeted delivery of anticancer drugs. The polymeric micelles demonstrated prolonged and pH/glucose-triggered drug release and enhanced cellular internalization by B16F10 cells through a receptor-mediated endocytosis pathway. The polymeric micellar system could inhibit the proliferation of B16F10 cells with IC50 values lower than those of unmodified micelles. In addition, the polymeric micellar system could markedly suppress cell migration, colony formation, and invasion and promote the apoptosis of B16F10 cells, indicating good anticancer efficiency in vitro. Therefore, this polymeric nanocarrier provides a potential platform for targeted anticancer therapy.
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