BMC PLANT BIOLOGY
·
2022
·Vol. 22
·期 1
·1-15
·
IF 5.26
Wang Haitang,Zhou Xuesong,Liu Chuchu,Li Weixi,Guo Wangzhen
Background In eudicots, germination begins with water uptake by the quiescent dry seed and is greatly related to the permeability of micropyle enriched callose layers. Once imbibition starts, seeds undergo a cascade of physiological, biochemical, and molecular events to initiate cellular activities. However, the effects of callose on water uptake and following seed metabolic events during germination are largely unknown. Cotton ( Gossypium hirsutum ) is a eudicot plant with natural fiber and edible oil production for humans. Here, we addressed this question by examining the role of GhGLU19 , a gene encoding β-1,3-glucanase, in cotton seed germination. Results GhGLU19 belongs to subfamily B and was expressed predominately in imbibed seeds and early seedlings. Compared to wild type, GhGLU19 -suppressing and GhGLU19 -overexpressing transgenic cotton lines showed the higher and lower seed germination percentage, respectively. Callose was enriched more at inner integument (ii) than that in embryo and seed coat in cotton seeds. In GhGLU19 -suppressing lines, callose at ii of cotton seeds was greatly increased and brought about a prolonged water uptake process during imbibition. Both proteomic and transcriptomic analysis revealed that contrary to GhGLU19 -overexpressing lines, the glycolysis and pyruvate metabolism was decreased, and abscisic acid (ABA) biosynthesis related genes were downregulated in imbibed seeds of GhGLU19 -suppressing lines. Also, endogenous ABA was significantly decreased in GhGLU19 -suppressing line while increased in GhGLU19 -overexpressing line. Conclusions Our results demonstrate that suppression of GhGLU19 improves cotton seed germination via accumulating callose of inner integument, modulating glycolysis and pyruvate metabolism, and decreasing ABA biosynthesis. This study provides a potential way for improving germination percentage in cotton seed production, and other eudicot crops.
研究领域:
植物学
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遗传学
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分子生物学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2019
·
IF 5.399
Zhen Qin,Si Lin,Yongjun Qiu,Qiming Chen,Yin Zhang,Jiachun Zhou,Liming Zhao
Immobilization of enzymes is an essential strategy with outstanding prospects in biocatalytic processes. Nontoxic, inexpensive immobilized enzyme approach is especially important for food enzymes. We here demonstrate that a carbohydrate-binding module family 56 domain (CBM56-Tag) mediates the immobilization of fusion enzymes with the curdlan (β-1,3-glucan) particle support, thereby enabling the one-step immobilization-purification of target enzymes. CBM56-Tag exhibits an immunoglobulin-like β-sandwich fold, which can be adsorbed by curdlan via hydrogen bond-mediated binding. The maximum adsorption capacity of a fusion chitosanase (CBM56-GsCsn46A) on curdlan is 50.72 mg/g. The immobilized enzyme could be directly used in the packed-bed reactor. This immobilization strategy utilizes a natural polysaccharide without any treatment, avoiding the negative environmental effects. Moreover, the one step immobilization-purification simplifies the purification step, which reduces the use of chemicals. Our study provides a nontoxic and inexpensive immobilization strategy for the biocatalytic reaction in food industry.
研究领域:
生物化学
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食品科学
·
酶工程
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产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2025
·
IF 12.5
Bingqian Nie,Jianle Chen,Wenkang Wang,Tong Cao,Xingqian Ye,Shiguo Chen,Zhiqiang Hou
Ganoderma lucidum samples from space-mutated strains transported by China's “Shenzhou” recoverable satellites, along with traditionally cultivated counterparts, were selected for this study. Polysaccharides from Ganoderma lucidum were extracted using a two-step sequential method integrating hot water and alkaline extractions. Liquid chromatography-mass spectrometry (LC-MS) was employed to characterize polysaccharide structures (monosaccharide composition and glycosidic linkages) and profile terpenoids, assessing the impact of space mutation. Results showed a significant increase ( p < 0.05) in polysaccharide and terpenoid contents, with polysaccharide yield rising from 178.59 % to 265.38 %. Space-mutated polysaccharides exhibited higher glucose content, altered monosaccharide composition, lower molecular weight, and an enriched β-1,3-glucan structure. They also demonstrated enhanced anti-inflammatory activity, underscoring the functional benefits of space-induced modifications. This study presents the first comprehensive analysis of structural alterations in Ganoderma lucidum polysaccharides induced by space mutation. It provides valuable scientific insights into the space breeding of medicinal plants and suggests that space mutation technology could serve as a promising strategy for developing functional foods from Ganoderma lucidum .
研究领域:
植物学
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药理学
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食品科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2023
·
IF 6.1
Zhen Qin,Sainan Yu,Kemin Zhang,Xiasen Wei,Junjie Li,Zheyi Zhang,Sibao Wan,Haiyan Gao
β-1,3-Glucan-degrading enzymes are widely used in fields such as food processing, plant protection, and breweries. In this work, we identified a glycoside hydrolase (GH) family 157 endo-β-1,3-glucanase (BsGlc157A) from Bacteroides sp. M27 and characterized its biochemical properties, structural model, and antifungal activity. Enzymological characterization indicated that BsGlc157A performs its optimal catalytic activity at pH 6.0 and 40 °C. BsGlc157A adopted the classic (β/α)8 TIM-barrel structure. Two catalytic residues, the nucleophile (Glu215) and the proton donor (Glu123), were confirmed via structural modeling and site-directed mutagenesis. Moreover, BsGlc157A hydrolyzed curdlan into a series of oligosaccharides with polymerization degrees 2–5 and exhibited inhibitory effects on the hyphal growth of typical fruit pathogenic fungi (Monilinia fructicola, Alternaria alternata, and Colletotrichum gloeosporioides), thereby illustrating effective biocontrol activity. These results revealed the catalytic properties and the application potential of GH family 157 β-1,3-glucanase, thus providing valuable biochemistry information about the group of carbohydrate-active enzymes.
研究领域:
生物化学
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酶学
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微生物学
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植物保护
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Zheyi Zhang,Sainan Yu,Jiawei Gu,Feilong Tan,Sibao Wan,Haiyan Gao,Zhen Qin
β-1,3-Glucanases have prospective applications in areas such as functional oligosaccharide preparation, plant protection, and breweries. In this study, a glycoside hydrolase (GH) family 17 β-1,3-glucanase (BbGlc17A) from Bacteroidetes bacterium from a microbial mat metagenome from the Great Salt Lake was identified. BbGlc17A catalyzed the hydrolytic conversion of laminarin into β-glucooligosaccharides with polymerization degrees of 3–8. The optimal catalytic conditions of BbGlc17A were pH 6.5 and 30 °C. In addition to its hydrolytic activity, BbGlc17A also exhibited transglycosidase activities, involving catalysis of the formation of new β-1,6-glycosidic bonds. BbGlc17A exhibits the classic (β/α)8 TIM-barrel structure and possesses an elongated catalytic groove, distinguishing it from other typical β-1,3-glucanases, which promote the forward direction of the transglycoside reaction. This effectively highlights the potential of the enzyme to convert β-1,3-glucan into mixed functional oligosaccharides. These results reveal the catalytic properties and the application potential of the GH family 17 β-1,3-glucanase and provide valuable information about the group of carbohydrate-active enzymes in biochemistry.
研究领域:
生物化学
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酶学
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微生物学
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产品关联
DOI
PubMed
高影响因子
SYNTHESIS-STUTTGART
·
2021
·Vol. 53
·期 14
·2435-2448
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IF 3.157
Xin Zhou,Qing Long,Dongwei Li,Jingru Gao,Qikai Sun,Shaozi Sun,Yong Su,Peng Wang,Wenjie Peng,Ming Li
Structurally defined and pure oligosaccharides corresponding to β-glucans have attracted great attention because of their potential properties as immunostimulating agents and as antigens of vaccine candidates. We herein describe a convergent synthesis of ready-to-conjugate tridecasaccharides composed of a β-1,3-glucan nonasaccharide backbone and a β-1,6-glucan tetrasaccharide branch. The assembly was achieved by employing trichloroacetimidate glycosylations and features the gram-scale preparation of the nonasaccharide backbone and installation of the tetrasaccharide branch involving orthoester rearrangement to the glycoside.
研究领域:
有机化学
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生物化学
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疫苗研究
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DOI
高影响因子
Foods
·
2021
·Vol. 10
·期 12
·2951
·
IF 4.35
Qian Li,Ying Zhao,Yanli Xie
Paeonol can effectively inhibitAspergillus flavus(A. flavus) via damaging cell walls. In this work, paeonol treatment remarkably destroyed both the outer amorphous layer and the inner fibrous layer of cell walls. Furthermore, FT-IR and XPS characterization showed that OH functional groups were altered and proteins in the outer layer were released. According to proteomic analysis, 605 proteins have been identified and annotated. The activities of β-1,3-glucan synthase and chitinase were prohibited and promoted, respectively, by paeonol treatment, however, the activities of β-1,3-glucanase and chitin synthase were not influenced. QRT-PCR results suggested thatFKSP,CHIIII, andCHIVgenes might be the antifungal targets of paeonol. In addition, paeonol can effectively restrain the pathogenicity ofA. flavuson peanut butter. This study provided a new elucidation on the mode of action of paeonol against cell walls ofA. flavus, facilitating the application of paeonol in the preservation of agricultural products.Keywords:paeonol;Aspergillus flavus;cell walls
研究领域:
微生物学
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真菌学
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食品科学
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药理学
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DOI
PubMed
高影响因子
BIOLOGICAL CONTROL
·
2018
·
IF 2.112
Yi Zhou,Wen Li,Jiaoke Zeng,Yuanzhi Shao
The yeast Debaryomyces nepalensis was evaluated for effectiveness against Colletotrichum gloeosporioides in mangoes ( Mangifera indica L.) cv. ‘Tainong No. 1’, and possible mechanisms of action involved in the inhibition were identified. The results showed that D. nepalensis could survive in mango wounds and produce a hydrolytic enzyme (β-1,3-glucanase). Scanning electron microscopy showed that the yeast produced biofilms on the mango surface and yeast cells attached to each other and became embedded in the pathogen hyphal walls. In addition, D. nepalensis competed for nutrients (FeCl 3 ) and competed for space with the pathogen (p < 0.05). Morever, D. nepalensis enhanced defense-related enzymes of wounded mangoes, including peroxidase, β-1,3-glucanase, chitinase and phenylalanine ammonia-lyase. In addition, gas chromatographic-mass spectrometry (GC–MS) analysis of volatile compounds (VOCs) produced by the yeast showed that VOCs can play important roles over short distances in the suppression of C. gloeosporioides. The solid-phase microextraction (SPME) coupled with GC–MS technique analysis revealed phenylethyl alcohol (29.62% relative peak area, RA) as the dominant VOC mainly produced by D. nepalensis , and it had a significant inhibitory effect on fungal mycelia. According to the results obtained here, the combination of different mechanisms of action of the yeast increases their ability to control C. gloeosporioides. The use of antagonistic yeast to control C. gloeosporioides may contribute to the integrated management of disease caused by this pathogen.
研究领域:
植物病理学
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食品科学
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微生物学
✓ 已验证
DOI
高影响因子
JOURNAL OF CONTROLLED RELEASE
·
2024
·
IF 10.5
Xiaoli Ling,Zhipeng Tian,Dong Chen,Yujia Zhang,Ziyan Dong,Dan He,Jiayu Li,Zhidi He,Jiaxin Li,Fang Chen,Qin He,Man Li
Self-adjuvanted vaccine delivery platforms possess potential for targeted delivery of antigens and initiation of potent immune responses. Although aluminum-containing adjuvants have been approved and widely used in human vaccines, their effectiveness in inducing Th1-type immune responses is far from satisfactory. To facilitate antigen delivery and activate potent antitumor immune responses, a self-adjuvanted nanovaccine (CPBG-Al@OVA) is constructed by functionalizing aluminum hydroxide with β-1,3-glucan, which recognizes pattern recognition receptors via Dectin-1. Carboxymethyl-phosphorylated β-1,3-glucan (CPBG) has been synthesized and optimized to achieve superior adjuvanticity while maintaining water solubility. CPBG then self-assembles with aluminum hydroxide and the targeted antigen, leading to the formation of a nanovaccine CPBG-Al@OVA. Owing to the favorable nanoscale size distribution, CPBG-Al@OVA effectively drains to the lymph nodes and is internalized by antigen-presenting cells (APCs) through Dectin-1-mediated endocytosis, activating the Syk and Raf1 signaling pathways and leading to upregulated TNFSF15 and OX40L expression to activate APCs. Following immunization, CPBG-Al@OVA activates potent CD8 + T cell and humoral responses to inhibit tumor growth. Notably, mice vaccinated with CPBG-Al@OVA showed extended survival, with more than 70 % of the mice surviving for over 50 days post-tumor challenge. Moreover, the CPBG-Al nanoparticle also possesses personalized immune-activating capacity by encapsulating tumor lysate as an antigen, specifically suppressing tumor growth. This strategy synergizes the adjuvant effects of aluminum and β-1,3-glucan, offering a platform for self-adjuvanted nanovaccine design with significant clinical translational potential.
研究领域:
免疫学
·
癌症研究
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疫苗学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2021
·Vol. 105
·期 12
·5147-5158
·
IF 4.813
Li Qian,Zhu Xiaoman,Xie Yanli,Zhong Yue
Abstract o -Vanillin is a natural product that has been widely applied in the food and pharmaceutical industries. In this study, we determined that o -vanillin can strongly inhibit the growth of Aspergillus flavus mycelia. However, the inhibition mechanism of o -vanillin is still elusive. The ultrastructural morphology of mycelia was injured, and the cell walls were destroyed. The OH functional groups on cell walls were altered, and the content of protein in mycelial cell walls was reduced by o -vanillin. The content of β-1,3-glucan in cell walls was significantly ( P < 0.05) reduced by o -vanillin in a dose-dependent manner, while chitin was not markedly affected. Moreover, o -vanillin led to an increase in the permeability of cell membranes. o -Vanillin also exhibited a promising antifungal effect on contaminated corn kernels. Therefore, o -vanillin inhibited the growth of mycelia by disrupting the integrity of cell walls and cell membranes. This study not only sheds light on the antifungal mechanism of o -vanillin but also indicates that it is a promising agent for the control of A. flavus infection. Key points o -Vanillin has strong inhibitory effects on A. flavus. o -Vanillin destroyed the integrity of cell walls and cell membranes. o -Vanillin could effectively inhibit the growth of A. flavus on corn kernels.
研究领域:
药理学
·
微生物学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2025
·
IF 4
Xuemei Lu,Ke Hu,Minggui Ou,Min Li,Xinchun Zhang,Xingyu Zhan,Xun Liao,Ming Li,Rongyu Li
The soft rot disease caused by Fusarium oxysporum leads to a significant reduction in the yield of Dendrobium officinale . However, research into the synergistic inhibitory effect of essential oils in D. officinale is extremely limited. In this study, we systematically investigated the direct and indirect inhibitory activity of carvacrol, citral and their combination against F. oxysporum , and their synergistic inhibitory mechanism. Carvacrol and citral exhibited significant direct and indirect inhibitory activity against F. oxysporum with EC 50 values of 54.37 mg/L and 119.32 mg/L (direct), 18.01 μL/(L•air) and 48.70 μL/(L•air) (indirect). Synergistic analysis revealed that the optimal synergistic toxicity of carvacrol and citral combination (Ca•Ci) against F. oxysporum was 10:1, with co-toxicity coefficient (CTC) of 131.57 and EC 50 value of 44.24 mg/L. Microscopy confirmed that the Ca•Ci led to more significant tip constriction, uneven surfaces and serious rupture of F. oxysporum mycelia than single compound. Transmission electron microscopy (TEM) showed that Ca•Ci also caused significant ultrastructural alterations to F. oxysporum , manifesting as cytoplasmic disorganization and partial organellar disintegration. Moreover, Ca•Ci dramatically increased the sensitivity of F. oxysporum to calcofluor white compared with a single compound. Ca•Ci also considerably upregulated the expression of chitinase-related gene ( FOXG _12882) and β -1,3-glucanase-related gene ( FOXG _10637) in F. oxysporum , resulting in higher chitinase and β -1,3-glucanase activity. However, it should be noted that carvacrol exerted a greater contribution than citral. In conclusion, the combination of carvacrol and citral greatly disrupted the cell wall integrity of F. oxysporum , thereby exhibiting a synergistic effect.
研究领域:
植物病理学
·
微生物学
·
植物化学
·
真菌学
·
农业科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2016
·Vol. 182
·期 1
·261-275
·
IF 1.606
Jiang Cheng,Song Jinzhu,Cong Hua,Zhang Junzheng,Yang Qian
A novel β-1,3-glucanase gene, designated Ccglu17A , was cloned from the biological control fungus Chaetomium cupreum Ame. Its 1626-bp open reading frame encoded 541 amino acids. The corresponding amino acid sequence showed highest identity (67 %) with a glycoside hydrolase family 17 β-1,3-glucanase from Chaetomium globosum . The recombinant protein Ccglu17A was successfully expressed in Pichia pastoris , and the enzyme was purified to homogeneity with 10.1-fold purification and 47.8 % recovery yield. The protein’s molecular mass was approximately 65 kDa, and its maximum activity appeared at pH 5.0 and temperature 45 °C. Heavy metal ions Fe 2+ , Mn 2+ , Cu 2+ , Co 2+ , Ag + , and Hg 2+ had inhibitory effects on Ccglu17A, but Ba 2+ promoted the enzyme’s activity. Ccglu17A exhibited high substrate specificity, almost exclusively catalyzing β-1,3-glycosidic bond cleavage in various polysaccharoses to liberate glucose. The enzyme had a Km of 2.84 mg/mL and Vmax of 10.7 μmol glucose/min/mg protein for laminarin degradation under optimal conditions. Ccglu17A was an exoglucanase with transglycosylation activity based on its hydrolytic properties. It showed potential antifungal activity with a degradative effect on cell walls and inhibitory action against the germination of pathogenic fungus. In conclusion, Ccglu17A is the first functional exo-1,3-β-glucanase to be identified from C. cupreum and has potential applicability in industry and agriculture.
研究领域:
微生物学
·
酶学
·
分子生物学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）