LANGMUIR
·
2016
·
IF 3.993
Yichuan Hu,Bo Liang,Lu Fang,Guanglong Ma,Guang Yang,Qin Zhu,Shengfu Chen,Xuesong Ye
In this study, a versatile fabrication method for coating enzyme-based biosensors with ultrathin antifouling zwitterionic polymer films to meet the challenge of the long-time stability of sensors in vivo was developed. Electrochemically mediated atom transfer radical polymerization (eATRP) was applied to polymerize zwitterionic sulfobetaine methacrylate monomers on the rough enzyme-absorbed electrode surfaces; meanwhile, a refined overall bromination was developed to improve the coverage of polymers on the biosensor surfaces and to maintain the enzyme activity simultaneously for the first time. X-ray photoelectron spectroscopy and atomic force microscopy were used to characterize the properties of the polymer layers. The antifouling performance and long-time stability in 37 °C undiluted bovine serum in vitro were evaluated. The results showed that the polymer brush coatings diminished over 99% nonspecific protein adsorption and that the sensitivity of the evaluated sensor was maintained at 94% after 15 days. The overall sensitivity deviation of 7% was nearly 50% lower than that of the polyurethane-coated ones and also much smaller than the current commercially available glucose biosensors. The results suggested that this highly controllable electrodeposition procedure could be a promising method to develop implantable biosensors with long-time stability.
研究领域:
生物传感器
·
聚合物化学
·
材料科学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
Food Frontiers
·
2025
·Vol. 6
·期 2
·1058-1078
·
IF 6.9
Haolin Zhang,Jingjing Zhang,Hui Cao,Petra Högger,Randolph Arroo,Mohamed A. Farag,Avi Shpigelman,Jianbo Xiao,Chunlin Li
A class of flavonoid aminated derivatives that had been consistently overlooked in literature was shown to be formed as artefacts in cell culture medium. In this study, 34 flavonoids from different subclasses were incubated in Dulbecco's modified Eagle's Medium (DMEM) at 37°C for 2 h to identify the reaction mechanism behind aminated derivatives formation from flavonoids. Baicalein, scutellarein, dihydromyricetin, (−)-gallocatechin (GC), (−)-epigallocatechin (EGC), (−)-GC gallate, and (−)-EGC gallate were found to yield corresponding nitrogenous derivatives in both DMEM and amino acid solution. The nitrogen source of these seven flavonoid -aminated derivatives was revealed to be amino acids in DMEM. The pyrogallol group of the flavonoids was a key structural motif, being first oxidized into quinone and then further reacting with amino acids (Strecker degradation) to yield aminated flavonoids and corresponding aldehydes. A slightly alkaline environment accelerated the amination of flavonoids, possibly via the formation of flavonoid quinone. These results provide the mechanistic evidence for the in vitro generation of flavonoid -aminated derivatives, yet to be tested using in vivo assays.
研究领域:
生物化学
·
药理学
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产品关联
DOI
高影响因子
Viruses-Basel
·
2025
·Vol. 17
·期 11
·1452
·
IF 3.5
Nuo Wang,Ping He,Bohan Xu,Hongping Wei,Junping Yu
Diagnosis of dengue virus infections typically relies on RT-PCR-based methods, for which reliable positive controls are essential. Viral RNA is an ideal positive control, but its inherent instability poses a major challenge. Herein, we report a simple and effective method for stabilizing dengue virus RNA by immobilizing it onto silk fibroin films (RNA-SFFs). We evaluated various substrate surfaces for RNA-SFFs preparation and found that the inner surface of sealable bags is optimal for uniform film formation and easy harvesting. Screening different silk fibroin concentrations revealed that even low concentrations (2.8%) effectively preserved RNA well and kept Ct constant for up to 16 days at 25 °C, 37 °C, and even 45 °C (extreme weather for transportations). Due to its rapid film formation and ease of peeling, 7% silk fibroin was selected. Notably, the RNA-SFFs demonstrated robust resistance to UV irradiation, with no significant Ct value changes after 4 h of exposure. Long-term stability testing at −20 °C, 25 °C, and 37 °C showed that dengue serotype 1–4 RNA-SFFs remained stable for the entire duration of the study—up to 56 weeks (approximately 14 months)—at all tested temperatures. These results demonstrate that RNA-SFFs are highly stable, portable, and practical as positive controls for dengue diagnostics, with strong potential for use in on-site and resource-limited settings.
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产品关联
DOI
PubMed
高影响因子
ADVANCED MATERIALS
·
2024
·Vol. 36
·期 25
·2400656
·
IF 27.4
Hanchen Wang,Yingtian Liu,Mingze Jiang,Yu-Xing Yao,Chunhua Hu,Chong Yan,Qiang Zhang,Luming Li
The advanced lithium-ion batteries that can tolerate zero-volt storage (ZVS) are in high demand for implantable medical devices and spacecraft. However, ZVS can raise the anode potential, leading to Cu current collector dissolution and solid-electrolyte interphase (SEI) degradation, especially at 37 °C. In this contribution, by quantitatively regulating the dosage of Li 6 CoO 4 cathode additives, controllable potential of the working anode under abusive-discharge conditions is demonstrated. The addition of Li 6 CoO 4 keeps zero-crossing potential (ZCP) and the potential of ZVS below 2.0 V (vs Li/Li + ) for LiCoO 2 |mesocarbon microbead cells at 37 °C. The capacity retention ratio (CRR) increases from 69.1% and 35.9% to 98.6% and 90.8% after 10 and 20 days of ZVS, respectively. The Cu dissolution and SEI degradation are effectively suppressed, while the over-lithiated cathode exhibits high reversible capacity after ZVS. The limiting conditions of long-term ZVS are further explored and a corresponding guide map is designed. When quantitatively regulating ZCP and the potential in ZVS, Cu dissolution, SEI degradation, and irreversible conversion of the cathode constitute the limiting conditions. This contribution designs the most reasonable potential range for ZVS protection at 37 °C, and realizes the best CRR record through precise potential regulation for the first time.
研究领域:
材料科学
·
能源存储
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2021
·
IF 5.89
Zhou Li-Jian,Guo Li-Bin,Wei Wei,Lv Zhi-Xiang,Zhang Ye-Wang
Chondroitin AC lyase (ChSaseAC) is one of the essential polysaccharides lyases in low molecular chondroitin sulfate production. In this work, a novel PrChSaseAC from Pedobacter rhizosphaerae was successfully cloned, expressed in Escherichia coli. After optimizing the induction, the recombinant PrChSaseAC could be expressed efficiently at 0.1 mM IPTG, 25°C, and 12 h induction. Then, it was purified with Ni-NTA affinity chromatography. The characterization of the purified PrChSaseAC showed that it had high specific activity and good storage stability, which would favor the production of low molecular weight chondroitin sulfate. It also displayed activity toward chondroitin sulfate C and hyaluronic acid. PrChSaseAC had the highest activity at pH 7.5, 37°C, 10 mM Ca2+, and 5 mg/ml of chondroitin sulfate A. Molecular docking of substrate and enzyme showed the interactions between the enzyme and substrate; it revealed that the enzyme showed high activity to CS-A and hyaluronic acid, but lower activity to CS-C attributed to the structure of the binding pocket. The high stability and specific activity of the enzyme will benefit the industrial production or clinical treatment.
研究领域:
生物化学
·
酶学
·
生物技术
·
药理学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF THERMAL BIOLOGY
·
2025
·
IF 2.9
Pengyun Qiao,Le Li,Zilong Li,Feifan Sun,Fa Ren
Sperm damage resulting from exposure to environmental toxicants or elevated temperatures frequently involves overlapping mechanisms, most notably the induction of mitochondrial oxidative stress. Lycium barbarum polysaccharide (LBP), a bioactive dietary component, mitigates decabromodiphenyl ether (BDE-209)-induced human sperm toxicity by modulating oxidative stress and apoptosis. Exposure to environmentally relevant levels of BDE-209 (40-80 μmol/L) significantly impaired sperm quality, as evidenced by reduced motility, compromised membrane and acrosome integrity, and increased DNA fragmentation when compared to the control group at 37 °C. Mechanistically, exposure to BDE-209 induces mitochondrial oxidative stress, resulting in reactive oxygen species (ROS) accumulation, decreased mitochondrial membrane potential (MMP), and reduced ATP production. This is evidenced by increased activities of Caspase3 and Caspase9, elevated levels of BAX, and decreased expression of BCL-2. LBP intervention (100 μg/mL) effectively scavenged ROS, restored mitochondrial potential, and enhanced antioxidant capacity, as demonstrated by increased superoxide dismutase (SOD) and glutathione (GSH) levels, alongside decreased malondialdehyde (MDA) levels. Furthermore, LBP suppressed apoptotic pathways and enhanced sperm-zona pellucida binding after BDE-209 exposure. A proposed mechanism summarizing this protective pathway is presented in the Graphical . As a naturally occurring polysaccharide that protects against oxidative stress in sperm, LBP could represent a promising candidate for dietary interventions in populations exposed to elevated levels of BDE-209. This warrants further investigation in in vivo models.
研究领域:
生殖毒理学
·
环境健康
·
氧化应激生物学
·
男科学
·
营养生物化学
·
线粒体生理学
✓ 已验证
DOI
PubMed
高影响因子
Food Packaging and Shelf Life
·
2020
·
IF 4.244
Hao Huang,Tarun Belwal,Li Li,Yi Wang,Halah Aalim,Zisheng Luo
Consumption of brown rice decreased the risk of chronic diseases, which might be due to the presence of phytochemicals. However, the aging process reduced phytochemical contents of brown rice. The present work investigated modified atmosphere packaging (MAP) of different oxygen levels to preserve cooking qualities and phytochemicals of brown rice during aging storage. Compared with other treatments, 90 % N 2 + 10 % O 2 packaging showed best effect of preserving cooking qualities including texture and microstructure. The oxidation level of brown rice was also the lowest in 90 % N 2 + 10 % O 2 packaging. After storage, contents of phytosterol (campesterol, stigmasterol, β-sitosterol) and phenolic compounds (gallic acid, p-coumaric acid, ferulic acid, caffeic acid, syringic acid, catechin, quercetin) of brown rice in 90 % N 2 + 10 % O 2 packaging were significantly higher than those in other packagings. These results indicated that 90 % N 2 + 10 % O 2 packaging was a suitable MAP method for the retention of acceptable cooking qualities and phytochemical contents of brown rice.
研究领域:
食品科学
·
营养学
·
采后技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）