TALANTA
·
2026
·
IF 6.7
Wangyu Nan,Qingdan Ye,Jie huang,Pei Liang,Qianmin Dong,Kun Pang,Zihong Ye,Kai Sun,Yufeng Wang
In this study, we developed an aptamer-mediated surface-enhanced Raman scattering (SERS)-coupled immunochromatographic assay (ICA) for the quantitative detection of pyrethroids. For this approach, core-shell gold nanoparticles (Au@Au NPs) were used as SERS probes by conjugating with aptamers, and fluorescent Raman reporter molecules (MPBN) were incorporated to yield stable, reproducible spectral signals. Leveraging the aptamers' specific recognition of the target analyte, Raman-labeled Au@Au NPs could be effectively captured by the test line of the immunochromatographic strip, thereby enabling the quantitative analysis of fenvalerate-with a limit of detection (LOD) as low as 0.4 ppb. Comparative experiments revealed that the detection sensitivity of this SERS-ICA method was 476 times higher than that of conventional lateral flow assays (LFA), showcasing a remarkable sensitivity advantage. To validate its practicality, spiked recovery experiments were conducted on three real samples, namely welsh onions, cowpeas, and kidney beans. The results showed that the spiked recoveries ranged from 78.3% to 112.5%, with relative standard deviations (RSD) between 1.7% and 20.1%. This fully confirms that the method possesses favorable specificity and accuracy, making it suitable for real-sample detection. This detection platform offers a new technical route for the rapid on-site monitoring of trace contaminants in food safety and environmental surveillance, holding broad application prospects.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2026
·
IF 6.7
Huixia Di,Zhouhao Lei,Jianing Li,Xiaochun Li
The accurate detection of hazardous pesticide residues is crucial for public health. Surface-enhanced Raman scattering (SERS) holds potential but faces practical limitations, including spectral overlap and matrix interference. To address these limitations, we developed a convolutional neural network (CNN)-assisted SERS platform with a hybrid substrate comprising a silver nanostar (AgNS) and hydrophobic silver nanoisland film (AgNIF). This platform synergizes localized surface plasmon resonance with a local concentration effect to achieve high sensitivity, demonstrating a broad linear range and low detection limits for nine pesticides. Coupled with an optimal data preprocessing protocol, our CNN model achieved superior classification accuracy: 99.44% for single pesticides, 98.47% for binary mixtures, 98.09% for ternary mixtures, and 94.60% in spiked tomato samples. Therefore, this work demonstrates a label-free, sensitive and accurate tool for pesticide detection and identification, holding great promise for guiding pesticide application and ensuring food safety.
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Science Advances
·
2026
·Vol. 12
·期 9
·
IF 13.9
Haoran Liu,Zihe Jiang,Zhiwei Hu,Banghuan Zhang,Tao He,Xiaohui Dong,Chaowei Sun,Jun Tian,Wei Jiang,Ferruccio Pisanello,Huatian Hu,Wen Chen,Hongxing Xu
Capturing molecular dynamics in nanoconfined channels with high spatiotemporal resolution is a key challenge in nanoscience, crucial for advancing catalysis, energy conversion, and molecular sensing. Bottom-up ultrathin plasmonic nanogaps, such as nanoparticle-on-mirror (NPoM) structures, are ideal for ultrasensitive probing due to their extreme light confinement, but their perceived sealed geometry has cast doubt on the existence of accessible transport pathways. Here, counterintuitively, we demonstrate that ubiquitous ligand-capped NPoM-type nanogaps can form a natural quasi–two-dimensional nanochannel, supporting molecular exchange and infiltration over unprecedented length scales (≳5 micrometers) with an extreme aspect ratio (>10 3 ). Using wavelength-multiplexed Raman spectroscopy, we resolve the underlying centripetal infiltration pathway with a spatial resolving power of ~20 nanometers. This redefines the NPoM architecture as a sensitive and hotspot-accessible platform, enabling in situ, real-time, reusable monitoring of analyte with ~10 −11 molar. This work establishes a versatile platform for advancing super-resolved in situ molecular sensing, nanoscale physicochemical studies, and on-chip nanophotofluidics.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Jiadan Zhang,Baiwen Wei,Jingwen Zhang,Pengzhao Wang,Jiefeng Rong,Fengfu Fu,Zhenyu Lin,Yongqiang Dong
Surface enhanced Raman scattering (SERS) hydrogel substrates face limitations in chemical enhancement (CM) due to the single-metal Fermi level, restricting analyte detection. Herein, negatively charged silver nanoparticles (AgNPs) and positively charged gold nanoparticles (AuNPs) were engineered into bimetallic hydrogel substrates (AgNPs@AuNPs-chips). The obtained AgNPs@AuNPs-chips exhibited a strong localized surface plasmon resonance (LSPR) peak matching the 785 nm laser for significant electromagnetic (EM) enhancement, while the dual Fermi levels (−4.26 eV for Ag, −5.10 eV for Au) enabled more photo-induced charge transfer (PICT) pathways for CM. Compared to monometallic AgNPs-chips with similar LSPR properties, the bimetallic chips demonstrated better synergistic EM/CM effects for some molecules. When applied to detect maleic hydrazide (MH), the chips achieved a limit of quantitation of 10 ppb, surpassing prior AgNPs-chip methods (100 ppb). Successful quantitative analysis of MH in real-world samples with good recovery demonstrated the substrate's practicality. This work pioneers dual-Fermi-level colloidal interfacial design, expanding SERS applicability in food safety.
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TALANTA
·
2026
·
IF 6.7
Qian Xie,Jinxin Chi,Zhixin Li,Limei Xu,Yujuan Xiao,Xucong Lin,Guihua Huang
The environmental risks of microplastics are exacerbated by the leaching of endocrine-disrupting additives such as bisphenol A (BPA). Effective monitoring requires analytical methods that are ultrasensitive, specific, and suitable for on-site application, requirements that remain challenging for conventional techniques. Herein, we develop an integrated capillary-based SERS aptasensor for the rapid and specific detection of BPA leached from microplastics. The sensor core is a three-dimensional hierarchical porous silica monolith (3D-PSM) anchored inside a quartz capillary. A DNA-programmed nanogap SERS hotspot matrix is constructed on the 3D-PSM by immobilizing cDNA-modified Au nanoparticles, followed by hybridization with aptamer-conjugated Au@4-MBN@Ag nanotags. This design utilizes the cDNA-aptamer duplex as a programmable molecular spacer to create a uniform sub-10-nm gap, generating an intense initial SERS signal (“signal-on”). Competitive binding of BPA triggers nanotag release and quantifiable signal attenuation (“signal-off”). Benefiting from convective mass transport within the 3D architecture, the platform achieves a limit of detection (LOD) of 1.24 pM for BPA, exhibiting a linear range spanning five orders of magnitude (1.0 pM to 0.1 μM), with a recognition time of 10 min. Practical utility was demonstrated by analyzing BPA thermally liberated (70 °C) from polycarbonate microplastics (PC-MPs, 100 and 1000 mesh) in environmental water matrices. The method exhibited high specificity and satisfactory recoveries (93.7 ± 3.6% to 102.0 ± 7.6%) in spiked river water samples, demonstrating a positive correlation between leached BPA amounts and MP contamination levels. This work provides a reliable tool for the direct assessment of hazardous chemical leaching from microplastics.
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MOLECULES
·
2022
·Vol. 27
·期 24
·8764
·
IF 4.927
Panxue Wang,Li Wang,Cen Li,Xiang Li,Guoliang Li
Reliable detection and quantification of antibiotic residues in food using surface-enhanced Raman spectroscopy remain challenging, since the intensities of SERS signals are vulnerable to matrix and experimental factors. In this work, a ratiometric SERS aptasensor using 6-Carboxyl-X-Rhodamine (ROX)-labeled aptamers and 4-mercaptobenzonitrile (4-MBN)-functionalized gold nanoparticles (Au NPs) as SERS probes was established for the reliable and rapid detection and quantification of enrofloxacin. In the presence of enrofloxacin, the conformational transform of aptamers took place, and the distance between ROX and Au NP increased, which resulted in a decrease in the SERS signal intensity of ROX. Meanwhile, the intensity of the SERS signal of 4-MBN was used as an internal standard. Reliable determination of enrofloxacin was realized using the ratio of the SERS signal intensities of ROX to 4-MBN. Under optimal conditions, the developed ratiometric SERS aptasensor provided a wide linear range from 5 nM to 1 µM, with a correlation coefficient (R2) of 0.98 and a limit of detection (LOD) of 0.12 nM (0.043 ppb). In addition, the developed ratiometric SERS aptasensor was successfully applied for the determination of enrofloxacin in fish and chicken meat, with recovery values of 93.6–112.0%. Therefore, the established ratiometric SERS aptasensor is sensitive, reliable, time-efficient, and has the potential to be applied in the on-site detection of enrofloxacin in complex matrices.Keywords:enrofloxacin;detection;SERS aptasensor;ratiometric;portable Raman spectrometer;food safety
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ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Sheng Chen,Mengya Lv,Jiayi Fan,Yanjie Huang,Gaolin Liang,Shusheng Zhang
Developing precise and effective strategies for cancer identification and imaging is attractive due to their importance for early cancer detection, prognosis, and subsequent treatment. Herein, we reported a novel bioorthogonal surface-enhanced Raman scattering (SERS) nanoprobe for accurate cancer cell imaging . A novel core-molecule-shell nanoflower (Au@4-MBN@Au) with rich electromagnetic hot spots and enhanced Raman scattering was first synthesized by optimizing the embedded concentrations of 4-mercaptobenzonitrile (4-MBN). Then, Au@4-MBN@Au was further modified with FA-PEG-SH molecules to acquire the bioorthogonal SERS nanoprobe Au@4-MBN@Au-PEG-FA. The SERS nanoprobe illustrated a robust and stable nitrile stretching vibration Raman signal (2223 cm −1 ) in the cellular silent region, ensuring high sensitivity and ultra-accuracy SERS imaging of cancer cells. Furthermore, cell imaging results demonstrated Au@4-MBN@Au-PEG-FA could recognize FR-positive HeLa cells with high selectivity due to the high affinity between folate receptor and folic acid. More notably, Au@4-MBN@Au-PEG-FA has been applied to identify FR-positive Hela cells from co-cultured cancer cells with similar morphology by SERS imaging for the first time. With improved signal-to-background ratio, high selectivity, and excellent stability, we anticipate the SERS nanoprobe Au@4-MBN@Au-PEG-FA could be applied for FR-related cancer theranostics and clinical detection in the future.
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ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Sheng Chen,Jiayi Fan,Mengya Lv,Chenfeng Hua,Gaolin Liang,Shusheng Zhang
Hydrogen sulfide (H2S), as the third endogenous gasotransmitter, is closely associated with various physiological and pathological processes, whereas many aspects of its functions remain unclear. Effective tools for the accurate detection of H2S in living organisms are urgently needed. We herein reported an internal standard assisted surface-enhanced Raman scattering (SERS) nanoprobe for ratiometric detection of H2S in vitro and in living cells based on the reduction of nitros with H2S. This nanoprobe consists of an internal standard (4-mercaptobenzonitrile, MPBN) embedded core-molecule-shell Au nanoflower (Au@MPBN@Au) as the high plasmonic active SERS substrate and the 4-nitrothiophenol (4-NTP) molecule immobilized on the surface as the H2S recognition unit. With the addition of H2S, the nitros peak (1329 cm–1) decreased. Meanwhile, three obvious new peaks appeared at 1139, 1387, and 1433 cm–1, which were related to the vibration of the dimerized product 4,4′-dimercaptoazobisbenzene (DMAB) of 4-aminothiophenol (4-ATP). However, the peak intensity at 2223 cm–1 derived from MPBN was not influenced by the outer environment. Thus, the H2S level was able to be determined based on the ratio of two peak intensities (I1139/I2223) with a detection limit as low as 0.24 μM. Notably, we have proved that SERS nanoprobe Au@MPBN@Au@4-NTP could ratiometrically image both the endogenous and exogenous H2S in living cells. We anticipate that Au@MPBN@Au@4-NTP could be applied for the study of H2S-related physiological function in the future.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Ailing Su,Yuan Liu,Xiumian Cao,Weiqing Xu,Chongyang Liang,Shuping Xu
A new gene detection platform based on surface-enhanced Raman spectroscopy (SERS) and clustered regularly interspaced short palindromic repeats (CRISPR)/Cas12a system is developed, which is applied for virus gene detections. CRISPR/Cas12a activated by CRISPR ribonucleic acid (crRNA) can recognize and lock the complementary target double-stranded deoxyribonucleic acid (dsDNA), followed by the cis -cleavage on the dsDNA and the trans -cleavage on the nearby linker single-stranded DNA (ssDNA). Target-induced trans -ssDNA cleavage leads to a failure in an aggregation behavior of the designed SERS probe pair that was bridged by the linker ssDNA, which causes the SERS intensity reduced relative to the control trial that gives much stronger SERS due to the severe aggregation state of SERS probes caused by intact linker ssDNA. This sensing method achieves the highly sensitive detections of human papillomavirus (HPV) genes in serum and pseudovirus within 40 min. The detection sensitivity of this CRISPR/Cas-SERS platform can reach pM. Owing to the flexible designs of the DNA probe and crRNA, this CRISPR/Cas-SERS sensing system is a universal gene detection tool, which can be applied for the in-vitro diagnosis field and point-of-care testing.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2022
·Vol. 414
·期 23
·6965-6975
·
IF 4.478
Chen Jiaming,Qu Xiaozhang,Qi Guohua,Xu Weiqing,Jin Yongdong,Xu Shuping
Electrostimulation (ES) is an important therapeutic method for diseases caused by abnormal intracellular electrical activity. Also, it can induce apoptosis of cells, which is a potential tumor treatment method. At present, there are no relevant studies on changes in intracellular reactive oxygen species (ROS) levels produced in the process of ES, or on the effects of simultaneous implementation of conventional antioxidant inhibitor drugs and ES therapy. To reveal these, two organelle-targeting core–shell plasmonic probes were designed for measuring ROS produced during ES. The probes were delivered into target organelles (nucleus and mitochondrion) before the cells were electrically stimulated for different periods of time. Surface-enhanced Raman scattering (SERS) signals were detected in situ , and the sensing mechanism for the quantitative analysis of ROS is based on the signal reduction of SERS caused by the ROS-etching effect on the silver shell. The detection results revealed that ES could trigger ROS generation in cells, and the ROS levels localized around organelles were assessed by SERS. This study has great potential for exploring abnormal organelle microenvironments via organelle-targeting probes combined with SERS technology. Graphical abstract
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NANOTECHNOLOGY
·
2021
·Vol. 33
·期 11
·115702
·
IF 3.874
Dan Zhu,Anran Li,Yunsong Di,Zhuyuan Wang,Jingzhan Shi,Xiaoqi Ni,Yiping Wang
The expression of membrane type-1 matrix metalloproteinase (MT1-MMP) in cancer cells is critical for understanding the development, invasion and metastasis of cancers. In this study, we devised an interference-free surface-enhanced Raman scattering (SERS) nanoprobe with high selectivity and specificity for MT1-MMP. The nanoprobe was comprised of silver core-silica shell nanoparticle with a Raman reporter tag (4-mercaptobenzonitrile) embedded in the interface. Moreover, the nitrile group in 4-mercaptobenzonitrile shows a unique characteristic peak in the Raman-silent region (1800–2800 cm−1), which eliminates spectral overlapping or background interference in the Raman fingerprint region (500–1800 cm−1). After surface modification with a targeting peptide, the nanoprobe allowed visualization and evaluation of MT1-MMP in breast cancer cells via SERS spectrometry. This interference-free, peptide-functionalized SERS nanoprobe is supposed to be conducive to early diagnosis and invasive assessment of cancer in clinical settings.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Xueliang Lin,Youliang Weng,Yi Liu,Duo Lin,Huishan Yang,Zhiwei Chen,Shangyuan Feng
The rapid and high precision analysis of SARS-CoV-2 RNA in saliva is crucial for the efficient prevention and control of COVID-19. Herein, we developed a novel ratiometric surface-enhanced Raman spectroscopy (SERS) sensing chip based on a two-dimensional core-molecule-shell-molecule nanostructure for quantitative detection of SARS-CoV-2 RNA. Owing to the use of base-complementary pairing mechanism, this SERS sensing chip has better selectivity in discriminating SARS-CoV-2 RNA against other RNA. Besides, an internal standard molecule (4MBN) in the SERS sensing chip can used to calibrate the real-time changing Raman signals of the probe molecule during quantitative assay. Using this cleverly designed SERS sensing chip, the dynamic ranges from 10 –6 M to 10 –12 M with a low of detection limit (LOD = 7.61 ×10 –14 M) can be acquired for detection of SARS-CoV-2 RNA. Meanwhile, this SERS sensing chip was well adapted for point-of-care testing (POCT) in combination with portable Raman spectrometer, which is expected to achieve effective detection of early SARS-CoV-2 infection.
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