Microorganisms
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2025
·Vol. 13
·期 9
·2088
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IF 4.2
Yingchao Sun,Yuxin Chai,Shuangming Li,Yongcheng Chen,Chunhui Ma,Rongzheng Huang,Fanfan Zhang
Background: Nettle is a potential non-conventional feed resource due to its high level of crude protein content, and ensiling is better for utilization in the animal industry. Previous integrated analysis (microbiome and metabolome) suggested that 4-pentenoic acid and malic acid in ensiled nettle may inhibit harmful microorganisms within the system. The present study investigated the effects of these two acids on nettle silage quality through the addition of 1% fresh weight of these acids, then analyzed the characteristics and bacterial communities during 60 days of nettle ensiling. Results: The addition of 4-pentenoic acid increased the content of crude protein (CP) and acetic acid (AA) compared with both control and malic acid-treated groups during 30 to 60 days of ensiling (p< 0.05). Lactic acid (LA) content was highest in the malic acid-treated group (4.21%, dry matter, DM based) compared to the control and 4-pentenoic acid-treated groups after 7 days of nettle ensiling (p< 0.05), but lower compared with the 4-pentenoic acid-treated group after 30 days of nettle ensiling (p< 0.05), and it was not detected in all groups after 60 days of silage. The contents of butyric acid (BA) and ammonia (AN) were the lowest (2.92–4.39% of DM and 9.94–24.28% of total nitrogen, respectively) in the 4-pentenoic-treated group compared with both control and malic acid-treated groups during 30 to 60 days of ensiling (p< 0.05). Both acids increased the relative abundance ofWeissellaafter 30 days of nettle ensiling, with 4-pentenoic acid showing a higher inhibitory capacity. Both acids showed a trend to inhibit the relative abundance ofClostridium sensu stricto 15after 30 days of nettle ensiling.Clostridium sensu stricto 15showed significant positive correlation with BA and AN (p< 0.05). Conclusions: The results of the present study suggested that the addition of 4-pentenoic could improve the quality of silage by reducing levels of protein degradation, probably resulting from its inhibited activity againstClostridiumspp. However, malic acid was less effective than 4-pentenoic acid in suppressingClostridiumspp. activity and the associated production of BA and AN, resulting in inferior preservation of CP.
研究领域:
动物科学
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微生物学
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植物科学
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生物化学
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生物技术
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Chengqiang Hu,Hongru Zhao,Ruiyi Sun,Xiumin Yang,Junlian Wang
Deep separation of Zr and Hf has always been a great challenging due to their very similar physicochemical properties. In this study, we established a method to synthesize two novel extraction chromatographic resins which graft dialkylphosphinic acid groups through amide group. The dialkylphosphinic acid modified resins were characterized by FT-IR, EA, SEM-EDS, XPS, and BET. Their adsorption and separation behaviors for Zr and Hf were investigated, and the adsorption mechanism was studied. The Zr and Hf adsorption onto Mer-CON-POOH-272 and Mer-CON-POOH-HYY2 both fit pseudo-second order model and Langmuir isotherm model. Mer-CON-POOH-HYY2 has stronger affinity to Zr and Hf than Mer-CON-POOH-272. Mer-CON-POOH-272 has a theoretical maximum loading capacity of 5.663 mg·g −1 Zr and 11.111 mg·g −1 Hf, while Mer-CON-POOH-HYY2 has a higher theoretical maximum loading capacity of 8.376 mg·g −1 Zr and 13.157 mg·g −1 Hf. Dialkylphosphinic acids are the functional groups of Mer-CON-POOH-272 and Mer-CON-POOH-HYY2 that interact with Zr and Hf during adsorption. They adsorb Zr and Hf through cation exchange mechanism, which can be expressed as M(SO 4 ) 3 2– + 2HL ⇌ M(SO 4 )L 2 +. 2H + + 2SO 4 2- . With increasing H 2 SO 4 concentration, the Zr and Hf extraction percentages and their separation efficiency reduce. The DFT calculation reveals that the adsorption process is accompanied by charge transfer. Adsorption occurs mainly at P = O and P-O positions. The affinity of the two resins for Hf is higher than that of Zr, which is the reason why the resin preferentially adsorbs Hf.
研究领域:
材料科学
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化学工程
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分析化学
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ANALYTICAL CHEMISTRY
·
2026
·
IF 7.3
Simeng Yan,Xindan Zheng,Jiawei Liu,Quan Bai
Chiral covalent organic frameworks (CCOFs) have potential application in enantioseparation due to their advantages, such as large surface area, abundant chiral recognition sites, and good chemical stability in organic solvents. However, due to the lack of essential active groups in COFs structures, it is challenging to introduce chiral ligands, which poses a challenge to the preparation and promotion of CCOFs. In this study, a strategy was developed to prepare CCOF@SiO2 core–shell microspheres for enantioseparation using the Ugi and click reactions. Imine-linked COF@SiO2 core–shell microspheres were synthesized in situ by using nonporous silica microspheres as the core and TAPB-DMTP-COF as the COF shell. This shell was formed by covalently conjugating 1,3,5-tris(4-aminophenyl)benzene (TAPB) and 2,5-dimethoxyterephthalaldehyde (DMTP). The carbon–carbon double bond was introduced into the COF framework through the Ugi multicomponent reaction in the presence of 4-pentenoic acid and cyclohexene isocyanate. The olefin-linked Ugi-COF@SiO2 microspheres were then reacted with thiolated chiral ligands such as β-CD-SH, captopril, and l-cysteine to produce CCOF@SiO2 core–shell microspheres via the “thiol–ene” click reaction. These CCOF@SiO2 microspheres were used as stationary phases to separate various racemic compounds in the normal phase and reversed-phase liquid chromatography modes. The columns with different chiral ligands showed excellent chiral recognition and separation capabilities for chiral compounds with good reproducibility. The successful applications highlight the potential of using the Ugi and “thiol–ene” click reactions to incorporate double bond active groups and different chiral ligands into the imine-linked COFs framework. This study’s proposed strategy is versatile and offers a broad platform for the preparation and applications of CCOFs in enantioseparation.
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Environmental Science & Technology Letters
·
2026
·
IF 7.3
Zhaozhao Gao,Meng Yan,Xingjia Xiang,Qing Wang,Yucheng Wu,Xin Song,Xiangui Lin,Frank E. Löffler,Jun Zeng
Colorimetric measurements of inorganic fluoride (F–) offer a rapid screening of biodefluorination, but existing methods fall short when they are applied to growing cultures. By systematically replacing mineral salt medium components, we demonstrate that a modified HEPES/Cl–-rich medium allows colorimetric F– quantification (20–200 μM) during biodefluorination of 1-fluorodecane (FD), 4,5,5-trifluoropent-4-enoic acid (TFEA), and 4,4,4-trifluoro-3-(trifluoromethyl) crotonic acid (SFCA). Applied in a deep 96-well microtiter plate format, this approach explored the biodefluorination of these compounds in growing axenic cultures, resting cell incubations, and mixed environmental cultures. Sphingopyxis sp. strain NJF-3 and Rhodococcus sp. strain NJF-7 exhibited differential defluorination of TFEA and SFCA. Resting cells exhibited limited defluorination activity to TFEA and SFCA, but strain NJF-7 displayed higher TFEA defluorination (219 μM F–) than strain NJF-3 (39.1 μM F–), indicating a greater growth-independent defluorination capacity. Defluorination by environmentally sourced microbial communities varied, potentially reflecting differences in sample origin (water vs soil). Correlation analysis of intermediates measured in different SFCA-defluorinating cultures indicated that abiotic and biotic reactions contribute to SFCA transformation, with a β-oxidation-type pathway inferred for the biotransformation. The refined approach expands the tool box for investigating biodefluorination in microbial enrichment and pure culture studies.
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ChemMedChem
·
2017
·Vol. 12
·期 19
·1600-1609
·
IF 3.225
Xinyu Hu,Yongmei Wang,Liangliang Zhang,Man Xu,Jianfa Zhang,Wei Dong
Graphical The power of attraction : Salecan-based composite hydrogels have magnetic-field-responsive properties due to the introduction of Fe 3 O 4 @SiO 2 nanoparticles into the hydrogel system. As a model anticancer drug, doxorubicin (DOX) was loaded into the composite hydrogels with high efficiency via strong electrostatic interactions between the amine group in DOX and carboxyl groups present in the hydrogels. These DOX-loaded salecan-g-PPH/Fe 3 O 4 @SiO 2 composite hydrogels show pH-responsive controlled drug release, and the liberated DOX can be effectively taken up by A549 cells. Salecan is a water-soluble extracellular β-glucan and has excellent physicochemical and biological properties for hydrogel preparation. In this study, a new pH/magnetic field dual-responsive hydrogel was prepared by the graft copolymerization of salecan with 4-pentenoic acid (PA) and N -hydroxyethylacrylamide (HEAA) in the presence of Fe 3 O 4 @SiO 2 nanoparticles for doxorubicin hydrochloride (DOX) release. Integration of Fe 3 O 4 @SiO 2 nanoparticles in salecan-g-poly(PA-co-HEAA) copolymers afforded magnetic sensitivity to the original material. DOX-loaded hydrogels exhibited a clear capacity for pH/magnetic field dual-responsive controlled drug release. Lowering the pH to acidic conditions or introducing an external magnetic field caused an enhancement in DOX release. This salecan-g-poly(PA-co-HEAA)/Fe 3 O 4 @SiO 2 composite hydrogel is a promising drug carrier for magnetically targeted drug delivery with enhanced DOX cytotoxicity against A549 cells.
研究领域:
材料科学
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药学
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生物医学工程
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WATER RESEARCH
·
2025
·
IF 12.4
Zhaozhao Gao,Xingjia Xiang,Meng Yan,Yongqi Liu,Yucheng Wu,Xin Song,Xiangui Lin,Frank E. Löffler,Jun Zeng
Microbial defluorination of per- and poly-fluoroalkyl substances (PFAS) likely involves cometabolic degradation. The isolation of pure cultures capable of this cometabolism would enhance our understanding of microbial defluorination mechanisms. Here, a novel isolate Sphingopyxis sp. strain NJF-3 was isolated and used to investigate the cometabolic defluorination of two poly-fluoroalkyl substances, 4,5,5-trifluoropent-4-enoic acid (TFEA) and 4,4,4-trifluoro-3-(trifluoromethyl)crotonic acid (SFCA). The isolate utilized 1-fluorodecane (FD) as the sole carbon source and released 873 ± 55 μM of inorganic fluoride (F⁻) from 1 mM FD within a 7-day incubation. The cometabolism of TFEA and SFCA by strain NJF-3 were significantly dependent on bacterial growth. Eleven cometabolic substrates were tested, revealing that 3,3-dimethylacrylic acid significantly stimulated TFEA defluorination, resulting in the removal of 27.3 ± 1.8 μM TFEA and the release of 71 ± 2.7 μM F⁻ over 28 days of incubation. Ammonium acetate promoted SFCA defluorination, leading to the degradation of 183 ± 17 μM SFCA and the release 736 ± 8.3 μM of F⁻ following 60 days of incubation. The integration of intermediate analysis with genomic data of strain NJF-3 revealed that the cometabolic biodefluorination proceeds via β -oxidation pathway, with hydroxylation-dehydration steps likely crucial for enzymatic C–F bond cleavage.
研究领域:
环境微生物学
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生物修复
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微生物代谢
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有机氟化学
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环境科学
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2023
·
IF 9.9
Tiantian Wang,Baoluo He,Shenghua Xue,Xin Chen,Shujuan Liu,Qian Ye,Feng Zhou,Weimin Liu
In this work, dopamine n -butenylamide (DBA) modified GLM nanodroplets were prepared via directional ultrasound of bulk liquid metal in ethanol aqueous solution as well as DBA self-assembly, followed by grafting with urea-based gelators via radical polymerization to obtain GLM-based supramolecular gelators (Gelator@GLM). The grafting gelators can impart their good compatibility between the GLM nanodroplets and the base oil, so that the Gelator@GLM nanodroplets can be dispersed in the base oil uniformly and stably for more than 3 weeks. Meanwhile, the tribological properties of Gelator@GLM nanodroplets was significantly enhanced, with a reduction of coefficient of friction (COF) and the wear volume of 41.18% and 92.13%, respectively, when compared with the base oil. Furthermore, Gelator@GLM additives exhibited stable lubrication performance even under variable temperature and frequency conditions. The synergistic effect of GLM nanodroplets and the gels generating a physical adsorption film and a chemically protective film (containing iron and chromium oxides, nitrides and carbides) can be credited with the improved tribological performance.
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CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Yuhong Cui,Baoluo He,Shenghua Xue,Zhuo Chen,Shujuan Liu,Qian Ye,Feng Zhou,Weimin Liu
Improving the tribological performance of water via designing nanomaterials as lubricant additive has garnered mounting interest. In this work, the poly-(N-isopropylacrylamide) microgel is grafted onto Fe 3 O 4 nanoparticles surface (Fe 3 O 4 @PNA) via free radical polymerization. Then, the Fe 3 O 4 @PNA can self-anchored on the MXene nanosheets via hydrogen bonding interaction to obtain MXene@Fe 3 O 4 @PNA composite. The as-prepared MXene@Fe 3 O 4 @PNA can effectively reduce friction and wear when used as a water-based lubricant additive. Compared with pure water, the average coefficient of friction (COF) and wear volume decrease by 61% and 78.7%, respectively. The lubrication mechanism was analyzed by focused ion beam technique-TEM (FIB-TEM) and X-ray photoelectron spectroscopy (XPS). The outstanding tribological performance of MXene@Fe 3 O 4 @PNA is attributed to the synergistic lubrication effect of MXene and Fe 3 O 4 @PNA. Interlayer shear of MXene and spherical Fe 3 O 4 @PNA can make MXene@Fe 3 O 4 @PNA play a full role of sliding friction and rolling friction mechanism, which can induce tribo-chemical reactions to form a protective film on the contact area of the friction pair. In addition, the hydrophilic groups of the MXene@Fe 3 O 4 @PNA can adsorb a large number of water molecules and form a hydration layer, reducing the agglomeration of nanosheets and acting as a boundary lubrication film. Moreover, the as-prepared MXene@Fe 3 O 4 @PNA exhibit excellent photothermal conversion performance in both underwater environment and dry state. Based on this characteristic, infrared light can be collected efficiently and stored quickly. Then, convert and utilize it. Therefore, the multifunctional MXene@Fe 3 O 4 @PNA has a broad application prospect as a water-based intelligent lubrication additive.
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TRIBOLOGY INTERNATIONAL
·
2022
·
IF 5.62
Baoluo He,Peng Wang,Qi Lu,Yixuan Du,Shujuan Liu,Qian Ye,Feng Zhou,Weimin Liu
Herein, a bioinspired molecule-dopamine n-butenylamide-was self-assembled on Gallium-based liquid metal (GLM) nanodroplets to introduce vinyl group, and the zwitterionic poly (2-methylacryloxyethyl phosphocholine) (PMPC) was grafted onto GLM nanodroplets via surface-initiated free radical polymerization to obtain hybrid PMPC-GLM microgel . The as-prepared PMPC-GLM microgel has good dispersion stability in aqueous solution (chemical stability > 2 months), and shows excellent antibacterial properties due to the hydration of PMPC and antibacterial effect of Ga 3+ . Moreover, the hydration layer endows PMPC-GLM microgel with satisfactory tribological properties as an aqueous lubricant additive , with the COF and wear rate reduced by 65% and 62%. In addition to hydration lubrication , the improved tribological performance is attributed to the formation of protective film on the worn surface.
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CARBOHYDRATE POLYMERS
·
2023
·
IF 11.2
Jianqun Wang,Wenxia Xu,Wenxuan Zhang,Junlong Da,Lixue Liu,Xiaowei Huang,Chubo Yang,Yuanbo Zhan,Han Jin,Ying Li,Bin Zhang
Hydrogels are widely used as wound dressings for wound healing, but when hydrogels absorb wound exudate, swelling occurs and compresses the surrounding tissue, affecting healing. A chitosan injectable (CS/4-PA/CAT) hydrogel based on catechol and 4-glutenoic acid was prepared to avoid swelling and promote wound healing. After cross-linking by UV light, pentenyl groups formed hydrophobic alkyl chains which give the hydrogel a hydrophobic network and thus control its swelling. CS/4-PA/CAT hydrogels retained their non-swelling for a long time in PBS solution at 37 °C. CS/4-PA/CAT hydrogels had good injectable and adhesive properties, and had a good killing effect on E. coli and S. aureus and could remove the bacterial biofilms of E. coli and S. aureus . CS/4-PA/CAT hydrogels had good in vitro coagulation function by absorbing red blood cells and platelets. When used in a whole skin injury model, CS/4-PA/CAT-1 hydrogel stimulated fibroblast migration, promoted epithelialization and accelerated collagen deposition to promote defect healing, and showed good hemostatic effects in liver and femoral artery defects in mice. In summary, the non-swelling injectable hydrogel with free radical scavenging, rapid hemostasis, and antibacterial effects would be a promising treatment for defect repair.
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