JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Mingliang Zhang,Weihao Zhu,Hongfei Liu,Kaihua Pan,Qian Li,Qian Zhu,Yanni Huang,Changchang Wang,Junqiang Hu,Mingli Jiang,Xin Yan,Qing Hong
Acetaminophen (APAP), an analgesic and antipyretic drug, is commonly detected in wastewater treatment plant (WWTP) effluents, surface water, and soil, indicating its status as an emerging environmental contaminant. In this study, we isolated a bacterium, Pseudomonas taiwanensis AP-1, capable of completely mineralizing APAP and utilizing it as the sole carbon source for growth. A newly identified metabolite, γ-glutamyl-4-aminophenol (γ-G4AP), was reported for the first time in the degradation of APAP by strain AP-1. Two amidases (ApaH1 and ApaH2), responsible for the conversion of APAP to 4-aminophenol (4-AP), were identified through a combination of genomic comparison, heterologous expression, and gene knockout. Notably, ApaH1 played a pivotal role in the degradation of APAP by strain AP-1. The catalytic triad of ApaH1 (K82-S161-S185) and ApaH2 (K85-S160-S184) were identified as by molecular docking and site-directed mutagenesis. Additionally, a gene cluster apd for the metabolism of 4-AP was also successfully identified in strain AP-1, consisting of the aniline dioxygenase gene cluster apdBCD1D2EF and the BT catabolic gene apdGH . Interestingly, the 4-AP metabolic gene cluster apd was highly conserved among other Pseudomonas strains capable of APAP degradation. Our results provide new insights into the mechanism of APAP biodegradation and strain AP-1 may be a promising bacterium for the bioremediation of APAP pollutions.
研究领域:
环境科学
·
微生物学
·
药物代谢
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DOI
PubMed
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2018
·Vol. 165
·期 11
·B491
·
IF 3.662
Junjuan Qian,Depeng Zhang,Lirong Liu,Yinhui Yi,Mwenze Nkulu Fiston,Odoom Jibrael Kingsford,Gangbing Zhu
As a novel nanomaterial after graphene, molybdenum disulfide (MoS2) has potential applications in electrochemical sensing and other fields, while the low electric conductivity of MoS2 limits its application significantly. In this paper, carbon spheres (CSs) wrapped with molybdenum disulfide (CSs@MoS2) novel nanostructure was successfully prepared via a green hydrothermal method and then used for firstly electrochemical sensing of 4-aminophenol (4-AP, one toxic pollutant). The morphology and structure of CSs@MoS2 were characterized by scanning electron microscopy, transmission electron microscopy, X-ray diffraction, Raman spectroscopy and electrochemical impedance spectroscopy. As an electrochemical sensor, CSs@MoS2 nanohybrids modified electrode exhibits excellent electrochemical sensing performance toward 4-AP analysis owing to the synergetic advantages of MoS2 and CSs. Under the optimized conditions, the detection limit of 4-AP obtained in this work is 3 nM and the linear range is 0.01–18.00 μM.
研究领域:
材料科学
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环境科学
·
分析化学
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DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2018
·
IF 6.042
Jian Sun,Jiahui Zhao,Xingfu Bao,Qifeng Wang,Xiurong Yang
Diethanolamine (DEA) has been extensively utilized as an alkaline buffer in current assays of alkaline phosphatase (ALP) activity in the past decades. While playing the role of a buffer, the chemical reactivity of DEA has been widely ignored in such assays. Herein, we report an interesting chromogenic interaction between DEA and 4-aminophenol (AP) in the presence of H2O for the first time, which inspires us to develop a novel DEA-participated ALP activity assay by using 4-aminophenyl phosphate (APP) as a substrate. This APP/DEA-based colorimetric approach has been proved to be comparable and even superior to the conventional p-nitrophenyl phosphate (pNPP)-based one, especially in the low ALP activity region, due to its higher sensitivity. The clear response mechanism and excellent sensing performance ensure that it can be further applied to determining ALP activity in real biological samples, screening potential ALP inhibitors in vitro, establishing ALP-enabled ELISA, and even fluorophore-assisted fluorescent ALP activity assay. It is demonstrated that this strategy not only possesses a good feasibility, but also exhibits a promising outlook for a series of ALP-related and -extended detections.
研究领域:
生物化学
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医学检测
·
酶学研究
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2015
·
IF 4.097
Fen-Ying Kong,Sai-Xi Gu,Jing-Yi Wang,Hai-Lin Fang,Wei Wang
We demonstrate herein a newly developed electrochemical sensor base on the reduced graphene oxide–titanium nitride (RGO–TiN) nanohybrid . The nanohybrid was prepared by mixing the TiN nanoparticles and GO dispersion and then treating with glucose and Zn foils. The morphologies and microstructures of the as-prepared nanohybrid were characterized by UV–vis absorption spectroscopy, X-ray photoelectron spectroscopy, transmission electron microscopy and Raman spectroscopy. The as-prepared RGO–TiN nanohybrid exhibited excellent electrocatalytic activity toward the simultaneous detection of acetaminophen (ACOP) and 4-aminophenol (4-AP). As a result, the linear detection ranges were 0.06–660 μM for ACOP and 0.05–520 μM for 4-AP, respectively, with the low detection limits of 0.02 μM for ACOP and 0.013 μM for 4-AP. Based on the excellent performance, it was demonstrated that the prepared RGO–TiN nanohybrid was a promising candidate for advanced electrode material in electrochemical sensing field and the as-prepared RGO–TiN nanohybrid may find more applications for the detection of various analytes.
研究领域:
材料科学
·
分析化学
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产品关联
DOI
高影响因子
ACS Omega
·
2024
·
IF 3.7
Weiming Gao,Dingwu Jiang,Yiming Zhang,Yao Li,Zhilong Xu,Runxi Han,Hao Tian,Hufei Dai,Qijing Lu,Cuiping Li
Detoxification of 4-nitrophenol (4-NP) to 4-aminophenol (4-AP) with high efficiency and dynamic performance is challenging for a polymeric catalyst. Herein, a series of conjugated microporous polyanilines (CMPAs), capable of efficiently catalytically reducing 4-NP, were synthesized based on the Buchwald–Hartwig cross-coupling reaction mechanism. By adjusting the types of linkers and the molar ratios of linker to core, CMPAs with different Brunauer–Emmett–Teller (BET) specific surface areas and reduction degrees were obtained and used as the catalysts in reducing 4-NP. The ultrahigh catalytic reduction efficiency (K = 141.32 s–1 g–1, kapp = 0.00353 s–1) was achieved when using CMPA-3-0.7 as the catalyst (prepared with 4,4′-diaminodiphenylamine as the linker and a 0.7:1 molar ratio of linker to core). The catalytic reduction performance exhibited a strong correlation with the reduction degree and BET specific surface area of CMPAs. Furthermore, they also exhibit excellent cycling stability and dynamic performance. The coexistence of a microporous structure and high BET specific surface area endowed CMPAs with an increased number of catalytic active centers. The reversible redox transformation of CMPAs in the presence of NaBH4 and air enabled self-healing (the oxidation units in CMPAs were reduced to reduction units by NaBH4, and the newly generated reduction unit in CMPAs was subsequently oxidized to its original state by the O2 in the air), leading to the reduction reaction of 4-NP proceeded continuously and stably. The aforementioned factors resulted in the high efficiency of CMPAs for reducing 4-NP to 4-AP, enhancing the practical application prospects of CMPAs in the detoxification of 4-NP wastewater.
研究领域:
材料科学
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环境科学
·
催化化学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 9
·1-9
·
IF 5.479
Li Xinli,Niu Zhiying,Lin Zhenfeng,Qin Yuan,Xu Jingxi,Huang Huayu,Wang Liancheng,Zhang Jianjian,Xu Zhigang
Boron nitride (BN) was used as a support and covered with an ionic liquid (IL) and tungsten disulfide (WS 2 ) nanoparticles to obtain an electrode for the determination of 4-aminophenol (4-AP). BN was prepared using a “solvent cutting” method, and the BN-IL-WS 2 nanocomposite was obtained by an ultrasonic method. BN and its hybrids were characterized by scanning electron microscopy, transmission electron microscopy and X-ray diffraction. When the BN-IL-WS 2 composites were coated on the surface of the electrode, the response to 4-AP was strongly amplified due to the strong synergetic effect between the three materials. The voltammetric response of the modified sensor (with a maximum at 0.29 V vs. Ag/AgCl) in solutions with a pH of 6 is linear in the 0.01–50 μΜ 4-AP concentration range, and the limit of detection is 3 nM. A modified glassy carbon electrode was applied for the determination of 4-AP in seawater and dispersions containing paracetamol tablets. The results were consistent with those obtained by HPLC.
Schematic representation of the voltammetric determination process of 4-aminophenol (4-AP). The electrochemical sensor based on the glassy carbon electrode modified with boron nitride (BN), ionic liquid (IL) and tungsten disulfide (WS 2 ) nanomaterials. They, exhibit an excellent performance compared with other electrodes.
研究领域:
分析化学
·
材料科学
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Dan Liao,Zhenhua Liu,Runmin Huang,Jingang Yu,Xinyu Jiang
A novel MXene/MOF derivative N-Ti 3 C 2 /PC composite was successfully synthesized through in-situ nucleation and conversion of ZIF-8 on 2D hierarchical Ti 3 C 2 MXene nanosheets. Due to the insertion of ZIF-8, the restacking of Ti 3 C 2 nanosheets was effectively avoided, thus its electrochemical properties was significantly improved. The obtained N-Ti 3 C 2 /PC composite was constructed for the detection of 4-aminophenol (4-AP) and acetaminophen (ACOP) simultaneously. The results showed that the as-fabricated electrochemical sensor owned excellent electrocatalytic activity for 4-AP and ACOP, which was attributed to the synergistic effect of N-Ti 3 C 2 and PC that accelerating the electron transfer on the electrode surface. Under optimum experimental conditions, the linear detection range was 1–150 μM for 4-AP and ACOP. The detection limits (LODs) for 4-AP and ACOP were evaluated to be 0.059 μM and 0.050 μM (signal-to-noise ratios, S/N = 3) respectively. Furthermore, the N-Ti 3 C 2 /PC electrochemical sensor was employed to determine 4-AP and ACOP in industrial wastewater and paracetamol tablets, and satisfying recoveries were obtained. Consequently, the N-Ti 3 C 2 /PC composite is expected to have a wide range of applications in electrochemical for detecting of environmental pollutants.
研究领域:
材料科学
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环境科学
·
分析化学
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产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Dong Zhang,Ping Yan,Hong Yu,Jing Sun,Shuyun Zhu,Xian-En Zhao
Development of accurate, convenient and portable methods for monitoring 4-aminophenol (4-AP) is extremely important because of its strong toxicity. Here, a ratiometric fluorescence sensor based on Ag + -enhanced luminescence of Tb-DNA complexes has been presented for the detection of 4-AP. The luminescence of Tb-DNA complexes is enhanced about 30 times by Ag + , which can trigger energy transfer from DNA to Tb 3+ more efficiently. In the presence of 4-AP, Ag + can be reduced into Ag 0 owing to its strong reducibility, inducing the decrease of Tb-DNA complexes at 545 nm remarkably. Besides, 4-AP exhibits intrinsic fluorescence at 375 nm under the same conditions. Therefore, ratiometric detection of 4-AP can be achieved using F 375 /F 545 as readout with a detection limit of 0.4 μM. Real water samples have been tsted to evaluate this method in practical application and satisfactory results have been obtained. Furthermore, the addition of 4-AP into Tb-DNA-Ag + system induces a visible color variation from green to bluish violet, enabling visual detection of 4-AP under the assistance of a smartphone.
研究领域:
分析化学
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环境科学
·
生物传感
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2020
·
IF 3.594
Hao Guo,Tian Fan,Wenqin Yao,Wenhu Yang,Ning Wu,Hui Liu,Mingyue Wang,Wu Yang
Herein, a novel electrochemical sensor based on ZIF-67/MWCNT-COOH/Nafion composite was designed and prepared for simultaneous detection of 4-aminophenol (4-AP) and acetaminophen (AMP). The electrochemical behaviors of the 4-AP and AMP on the ZIF-67/MWCNT-COOH/Nafion electrode were explored via cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The results exhibited that the designed electrochemical sensor possessed superior electrocatalytic activity for 4-AP and AMP, which was attributed to the highly ordered porous structure of ZIF-67, the outstanding electrical conductivity and high electrocatalytic activity of MWCNT-COOH. Under optimization conditions, the DPV response of the prepared electrode depicted a wider linear response concentration range of 0.2 to 200 μM for 4-AP, and 0.1 to 100 μM for AMP, respectively. And the lower detection limits (LOD) of 0.01 and 0.07 μM were obtained for 4-AP and AMP, respectively. More importantly, the acquired electrode was applied to Yellow river water and Paracetamol tablets for analysis of 4-AP and AMP, which exhibited satisfying recovery of 98.2% ~ 106.7% for 4-AP and 94.7% ~ 99.6% for AMP, respectively. In a word, the constructed electrochemical sensing platform delivered a promising potential in real application.
研究领域:
分析化学
·
环境科学
·
药物分析
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DOI
高影响因子
MICROCHIMICA ACTA
·
2014
·Vol. 182
·期 3
·823-829
·
IF 3.719
Wang Jinshou,Shi Zhen,Jin Jing,Liu Qi,Zhang Shenghui
A three-dimensionally ordered macroporous (3DOM) film of polycysteine (poly-Cys) was used to modify a glassy carbon electrode (GCE) to result in a sensor for 4-aminophenol. The new electrode was used to study the behavior of 4-aminophenol by cyclic voltammetry and differential pulse voltammetry. In comparison to a bare GCE and a GCE modified with poly-Cys without using template, this electrode displays a larger peak current which may be attributed to the structure of poly-Cys and the large surface area of the macroporous structure. The anodic peak current at a working voltage of 195 mV is linearly related to the concentration of 4-aminophenol in two concentration intervals: 0.02 to 20 μM and 20 to 200 μM, respectively. The detection limit is 8 nM (at an S/N ratio of 3). The method was successfully applied to the determination of 4-aminophenol in spiked water samples.
A glassy carbon electrode modified with three-dimensionally ordered macroporous polycysteine film was developed to serve as a sensor for determination of 4-aminophenol. The modified electrode presents lower detection limit, wider linear range and long-term stability for the determination of 4-aminophenol.
研究领域:
分析化学
·
电化学传感器
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产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2023
·Vol. 47
·期 45
·20838-20845
·
IF 3.3
Wenli Hou,Xianyu Kang,Jiajie Lin,Mengying Xie,Yancai Li
In this work, bagasse derived nitrogen-doped activated carbon (B-dNAC) was prepared using waste sugarcane as a carbon precursor via a KOH-urea activated method. Then, gold nanoparticles were successfully loaded onto the B-dNAC and gold nanoparticles/B-dNAC nanocomposites (AuNPs/B-dNACNs) were obtained through the incorporation of chloroauric acid by a facile thermal reduction process. The structural composition and the morphology of the composites were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), transmission electron microscopy (TEM) and X-ray photoelectron spectroscopy (XPS). A AuNPs/B-dNACNs modified glassy carbon electrode (AuNPs/B-dNACNs/GCE) was constructed. The electrochemical behavior of 4-aminophenol (4-AP) on the AuNPs/B-dNACNs/GCE was investigated by differential pulse voltammetry (DPV) and cyclic voltammetry (CV). The AuNPs/B-dNACNs/GCE exhibited good electrocatalytic performance for the electrooxidation of 4-AP and can be used as a novel electrochemical sensor for the detection of 4-AP. Under optimal conditions, the linear range of 4-AP was 1–400 μM, with a low detection limit of 0.61 μM. In addition, it displayed good selectivity, reproducibility and stability. Most importantly, the AuNPs/B-dNACNs/GCE could be used for the determination of 4-AP in acetaminophen tablets, and good recovery was obtained in the range of 97.1%–104.9%.
研究领域:
材料科学
·
分析化学
·
环境科学
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产品关联
DOI
高影响因子
ACS Applied Nano Materials
·
2025
·
IF 5.5
Caiwen Pan,Qinghong Shi,Xiaoyan Dong,Linling Yu,Yan Sun
Detoxification of the typical organophosphate pesticide methyl parathion (MP) is of great significance for environmental remediation and sustainable development. Hydrolysis of MP using organophosphate hydrolase (OPH) is an effective and environmentally friendly way to degrade MP, but the hydrolytic product, 4-nitrophenol (4-NP), is still toxic. The cascade conversion of MP to 4-NP followed by reduction to a low toxic product, 4-aminophenol (4-AP), is thus an attractive way to detoxify MP. Herein, we fabricated a highly active reduction-catalytic cupric metal–organic framework (MOF) (Cu-AZO), which exhibited over 6-fold higher activity than the previous Cu-AZB. Thus, a chemoenzymatic catalyst (OPH/Cu-AZO) for one-pot cascade MP conversion to 4-AP was constructed by in situ immobilization of the OPH in Cu-AZO. OPH/Cu-AZO displayed an enzyme loading capacity of 16–69 mg/g with an activity recovery of up to 252%, and an average cascade conversion rate of up to 41.7 μmol·min–1·gcatalyst–1. At the optimized conditions, OPH/Cu-AZO completely converted 50 μM MP to 4-AP within 3 min at a low reductant concentration (10 mM NaBH4) in the one-pot cascade reaction. Moreover, the hybrid catalyst retained 99% activity in 10 days storage and >65% activity in 10 recycles. The results proved that OPH/Cu-AZO was the most efficient chemoenzymatic catalyst reported so far in the cascade conversion of MP to 4-AP, underscoring its potential applications in the detoxification of MP.
研究领域:
环境科学
·
生物催化
·
材料科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）