BIOSENSORS & BIOELECTRONICS
·
2016
·
IF 7.476
Juan Tang,Yapei Huang,Cengceng Zhang,Huiqiong Liu,Dianping Tang
A new impedimetric immunosensor for the fast determination of ochratoxin A (OTA) in food samples was developed based on the instant catalyst as enhancer. Initially, the signal tags were prepared via co-immobilization of anti -OTA antibody and amine-terminated dendrimer (PAMAM) on the graphene oxide nanosheets through the covalent interaction, which were utilized as a good platform for combining manganese ion ( anti -OTA-GO-PAMAM-Mn 2+ ). Upon target OTA introduction, a competitive-type immunoreaction was implemented between the analyte and the immobilized OTA-BSA on the electrode for the anti -OTA antibody on the graphene oxide nanosheets labels. After a competitive immunoassay format, the anti -OTA-GO-PAMAM-Mn 2+ were captured onto the electrode surface , which could induce the in situ formation of MnO 2 via classical redox reaction between Mn 2+ and KMnO 4 on the immunesensing platform. Moreover, the generated MnO 2 nanoparticles act as efficient catalyst could catalyze the 4-chloro-1-naphthol (4-CN) oxidation without H 2 O 2 to generate an insoluble precipitation on the platform. Under the optimal conditions, the instant catalyst based impedimetric immunosensor displayed a wide dynamic working range between 0.1 pg mL −1 and 30 ng mL −1 . The detection limit (LOD) of the assay was 0.055 pg mL −1 . The developed method exhibited high selectivity and can be used for the determination of OTA in real red wine samples.
研究领域:
食品科学
·
分析化学
·
生物传感器
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2013
·
IF 5.437
Li Hou,Zhuangqiang Gao,Mingdi Xu,Xia Cao,Xiaoping Wu,Guonan Chen,Dianping Tang
A highly sensitive and selective impedimetric immunosensor with triple signal amplification was designed for ultrasensitive detection of prostate-specific antigen (PSA) by using anti-PSA antibody and DNAzyme-functionalized gold–palladium hybrid nanotags (Ab 2 –AuPd–DNA). The signal was amplified based on the Ab 2 –AuPd–DNA toward the catalytic precipitation of 4-choloro-1-naphthol (4-CN). DNAzyme (as a kind of peroxidase mimic) could catalyze the oxidation of 4-CN, whilst AuPd hybrid nanostructures could not only provide a large surface coverage for immobilization of biomolecules but also promote 4-CN oxidation to some extent. The produced insoluble benzo-4-chlorohexadienone via 4-CN was coated on the electrode surface , and hindered the electron transfer between the solution and the electrode, thereby increasing the Faradaic impedance of the base electrode . Three labeling strategies including Ab 2 –AuNP, Ab 2 –AuPd and Ab 2 –AuPd–DNA were investigated for determination of PSA, and improved analytical features were obtained with the Ab 2 –AuPd–DNA strategy. Under optimal conditions, the dynamic concentration range of the impedimetric immunosensor spanned from 1.0 pg mL −1 to 50 ng mL −1 PSA with a detection limit of 0.73 pg mL −1 . Intra- and inter-assay coefficients of variation were below 8.5% and 9.5%, respectively. Importantly, no significant differences at the 0.05 significance level were encountered in the analysis of 6 clinical serum specimens and 6 diluted standards between the impedimetric immunosensor and the commercialized electrochemiluminescent method for PSA detection.
研究领域:
生物传感器
·
纳米技术
·
分析化学
·
癌症诊断
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Ximei Zhang,Lanzhen He,Guangmin Zhang,Fenying Kong,Lanlan Chen
Background Escherichia coli ( E. coli ) is a model microorganism extensively used in microbial research due to its well-characterized physiology and genetic tractability. Monitoring its physiological indicators, such as metabolic activity and stress responses, is essential for understanding microbial life processes. However, existing methods often lack sensitivity, rapidity, and biocompatibility. This study addresses these limitations by combining electrochemiluminescence (ECL) biosensing with a biocompatible interface, to enable precise and efficient monitoring of E. coli 's physiological state. Results This study presents a novel ECL biosensor that leverages the biocompatibility of bovine serum albumin (BSA) with E. coli . The biosensor employs horseradish peroxidase (HRP) and 4-chloro-1-naphthol (4-CN) to induce a precipitation reaction that quenches the ECL signal of CdS–K 2 S 2 O 4 , enabling the sensitive detection of endogenous hydrogen peroxide (H 2 O 2 ) produced by E. coli . The sensor exhibits excellent linearity, stability, and reproducibility, with a detection limit of 54 nM for H 2 O 2 . Additionally, the biosensor effectively distinguishes between antibiotic-resistant and sensitive strains of E. coli , demonstrating its potential for comprehensive physiological assessment. Significance This innovative ECL biosensor represents a significant advancement in microbial monitoring, combining high sensitivity, rapidity, and biocompatibility, offering a powerful tool for comprehensive physiological monitoring in bioanalytical sciences. Its potential applications in biotechnology, food industry, and drug research highlight its practical relevance.
研究领域:
生物分析科学
·
微生物学
·
生物传感
·
电化学
·
抗菌素耐药性
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2019
·
IF 9.518
Di Huang,Lu Wang,Yi Zhan,Lina Zou,Baoxian Ye
A novel signal on-off type photoelectrochemical (PEC) biosensing system was designed for sensitive detection of carcinoembryonic antigen (CEA) based on tin disulfide nanosheets loaded on reduced graphene cxide (SnS 2 -GR) as the photoactive material and gold nanoparticles coated on reduced graphene oxide-functionalized copper sulfide ( [email protected] ) for signal amplification . It's the first time for SnS 2 -GR was exploited as a sensing matrix. Here, the photocurrent signals of SnS 2 were amplified attributed to the sensitization effect of graphene. As signal amplifier, [email protected] could quench the photocurrents of SnS 2 -GR not only through the p-n type semiconductor quenching effect as well as the steric hindrance effect, but also as peroxidase mimetics to catalyze the oxidation of 4-Chloro-1-naphthol (4-CN) to produce insoluble product on the electrode surface . Based on the multiple signal amplification ability of [email protected] , CEA was detected sensitively with a linear range from 0.1 pg mL −1 to 10 ng mL −1 and limit of detection down to 59.9 fg mL −1 (S/N = 3). Meanwhile, the PEC biosensor displayed excellent performance in the assay of human serum sample, showing good application prospects for various target analysis.
研究领域:
生物传感
·
纳米技术
·
分析化学
·
癌症诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2022
·
IF 12.545
Siyu Luo,Fanglei Liu,Siyu Gu,Ke Chen,Guohai Yang,Yingqiu Gu,Juntao Cao,Lu-Lu Qu
Food-borne pathogens are one of the leading causes of food poisoning, which vigorously affect food safety and human health. Therefore, the development of early and rapid detection methods for food pollution evaluation is the key to food safety and quality control. Herein, a simple and inexpensive photoelectrochemical (PEC) sensor is developed for highly selective and ultrasensitive detection of Staphylococcus aureus (S. aureus). The technique is based on “signal-off” that employs Cu–C 3 N 4 –TiO 2 heterostructures as photoactive materials and monolayer Cu–C 3 N 4 nanozyme as a signal amplifier. In the presence of S. aureus , the aptamer-modified Cu–C 3 N 4 (Cu–C 3 N 4 @Apt, a signal amplifier) and S. aureus were specifically anchored on the surface of the ligand-modified photoelectrode . The Cu–C 3 N 4 @Apt nanozyme acted as a peroxidase to catalyze the oxidation of 4-chloro-1-naphthol (4-CN) to produce insoluble precipitate on the electrode surface and resulted in a significant decrease in photocurrent . Based on the signal-amplification by the Cu–C 3 N 4 @Apt nanozyme, the constructed PEC sensor demonstrated a wide linear range between 10–10 8 CFU/mL for the S. aureus detection with the detection limit (LOD) as low as 3.40 CFU/mL. Furthermore, the PEC sensor was capable of determining S. aureus in spiked orange juice and milk, with the recovery of 91%–113%, indicating the reliability of the sensor for S. aureus detection in real samples. This investigation provides a feasible strategy for the design of highly selective and ultrasensitive PEC sensors to determine analytes in complex systems.
研究领域:
分析化学
·
生物传感器
·
食品安全
·
纳米技术
·
光电化学
✓ 已验证
DOI
PubMed
高影响因子
ANALYST
·
2023
·Vol. 148
·期 12
·2855-2860
·
IF 4.2
Bizhen Huang,Jing Ran,Ruishen Li,Wei Zhuang,Jiabi Chen,Haixin Guo
Early diagnosis of acute myocardial infarction (AMI) can significantly reduce mortality, which can be achieved by the highly sensitive detection of AMI-specific cardiac troponin I (cTnI) biomarkers. Herein a highly sensitive and portable photoelectrochemical (PEC) immunoassay for the detection of cTnI was innovatively fabricated based on an efficient photocurrent response of Cu2O coupling with a split-type enzymatic biocatalytic precipitation reaction with a digital multimeter readout. Initially, Cu2O cubic nanomaterials with a good photocurrent response were obtained by facile green room temperature synthesis and dropped on fluorine-doped tin oxide (FTO) plates as photoactive materials (Cu2O/FTO). In the presence of target cTnI, horseradish peroxidase (HRP) on the detection antibody catalyzes the substrate 4-chloro1-naphthol (4-CN) to produce insoluble precipitates of benzo-4-chlorohexadienone (4-CD) on Cu2O/FTO with the assistance of H2O2, resulting in a decrease in the photocurrent of the Cu2O/FTO electrode. To make the whole inspection process more concise and efficient, we used a flashlight and a digital multimeter as the excitation light source and reading device for the PEC sensing system, respectively. Under the optimal conditions of immunoreaction time, pH, and loading, the photocurrent of Cu2O/FTO decreased with increasing target cTnI concentration with a limit of detection (LOD) of 4.7 pg mL−1 in the operating range of 0.01–10 ng mL−1. It is of interest that the developed portable PEC immunoassay is also capable of detecting cTnI in human serum samples with acceptable accuracy compared to the reference cTnI enzyme-linked immunosorbent assay (ELISA) method while maintaining portability and sensitivity.
研究领域:
生物医学工程
·
分析化学
·
心脏病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2017
·Vol. 10
·期 1
·51-58
·
IF 1.9
Xiao-Li Du,Tian-Fang Kang,Li-Ping Lu,Shui-Yuan Cheng
A novel electrochemiluminescence (ECL) biosensor based on CdSe@CdS quantum dots (QDs) functionalized MoS2 modified electrode was developed for sensitive detection of Pb(II) using HRP-mimicking DNAzyme biocatalytic precipitation (BCP) for ECL signal quenching. Polycationic poly(diallyldimethylammonium chloride) (PDDA) and negatively charged QDs were adsorbed on the surface of MoS2, in sequence, to form the MoS2–QDs composites, which were employed as a matrix for the immobilization of the G-rich DNA strand, i.e., T30695. The MoS2–QDs were characterized by UV-vis absorption spectroscopy, fluorescence spectroscopy, energy dispersive spectrometry, transmission electron microscopy, and field emission scanning electron microscopy. CdSe@CdS QDs can produce an ECL signal using 0.1 M K2S2O8 as a co-reactant. T30695 can combine with Pb(II) ions to form a stable parallel G-quadruplex. The G-quadruplex can further combine with hemin to form DNAzyme, which can catalyze H2O2 and oxidize 4-chloro-1-naphthol (4-CN) to produce an insoluble precipitation of benzo-4-chlorohexadienone on the surface of the sensor. When the concentration of Pb(II) in the solution was increased, more of DNAzyme was generated and subsequently more 4-CN molecules were oxidized catalytically, leading to a decrease of the ECL signal. Using this quenching strategy, the ECL signal of the biosensor was linearly dependent on the logarithm of the Pb(II) concentration ranging from 1.0 × 10−15 to 1.0 × 10−11 M with a detection limit of 0.98 f M. The stability and selectivity of the sensor were also investigated. The sensor was applied in the determination of Pb(II) in the real water samples and satisfactory results were obtained.
研究领域:
分析化学
·
环境科学
·
纳米技术
·
生物传感器
✓ 已验证
产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2020
·Vol. 45
·期 2
·915-922
·
IF 3.288
Jianming Zhang,Jia Lin,Tingjin Zheng,Yancheng Jiang,Shimu Luo,Yao Lin,Zhishan Zhang
A novel capacitance immunosensor based on DNAzyme concatemer-amplified signal-generation tags was developed for the sensitive detection of interleukin-6 (IL-6) on an interdigitated micro-comb electrode. To construct such an immunosensor, monoclonal mouse anti-human IL-6 antibodies were covalently conjugated onto a bovine serum albumin (BSA)-coated electrode using the glutaraldehyde cross-linker. Gold nanoparticles heavily functionalized with primer DNA (for the subsequent rolling cycle amplification; RCA) and polyclonal rabbit anti-human IL-6 antibodies were used for the development of a sandwich-type immunoassay on the modified interdigitated electrode in the presence of the target IL-6. Thereafter, the attached primer DNA with the immunocomplex triggered the RCA reaction for the formation of numerous DNAzyme concatemers with the assistance of hemins and G-rich DNA probes. DNAzyme concatemers catalyzed 4-chloro-1-naphthol into insoluble benzo-4-chlorohexadienone and were deposited onto the electrode, thus resulting in the local capacitance variation of the interdigitated micro-comb electrode. The performance and external factors affecting the analytical properties were studied in detail. Under optimum conditions, the capacitance variations of the immunosensor were proportional to the IL-6 concentration in the dynamic range of 1.0–1000 pg mL−1 at a detection limit of 0.68 pg mL−1. Good reproducibility, high specificity and acceptable storage stability were acquired for the analysis of the target IL-6. Moreover, the capacitance immunosensor was utilized to determine IL-6 in human serum specimens and gave well-matched analytical results in reference to the human IL-6 ELISA kit.
研究领域:
生物化学
·
分析化学
·
纳米技术
·
生物传感器
✓ 已验证
产品关联
DOI
高影响因子
ELECTROCHIMICA ACTA
·
2019
·
IF 5.383
Jialun Chen,Ping Tong,Lingting Huang,Zhonghua Yu,Dianping Tang
An interdigitated capacitance immunosensing system based enzymatic biocatalytic precipitation on micro-comb electrode was designed for the sensitive detection of prostate-specific antigen (PSA) by coupling Ti 3 C 2 MXenes with tyramine signal amplification strategy. The immunosensor was prepared by immobilizing anti-PSA capture antibody on MXenes-coated interdigitated electrode, whereas gold nanoparticles heavily functionalized with horseradish peroxidase (HRP) and detection antibody were utilized as the signal-transducer tags. Introduction of interdigitated electrode was expected to enhance the sensitivity of capacitance immunosensor. This system mainly consisted of the sandwich-type immunoreaction, formation of tyramine-HRP repeats on gold nanoparticle and enzymatic biocatalytic precipitation. The concatenated HRP through the tyramine oxidized numerous 4-chloro-1-naphthol molecules into insoluble benzo-4-chlorohexadienone with the help of H 2 O 2 , and coated the modified immunosensor to keep free ions away from the electrode, thus causing the local alteration in the capacitance. Under optimum conditions, the change of the immunosensor in the capacitance increased with the increasing target PSA concentrations from 0.1 ng mL −1 to 50 ng mL −1 at a detection limit of 0.031 ng mL −1 . Moreover, the interdigitated capacitance immunosensor showed good reproducibility, high specificity and acceptable accuracy for the analysis of human serum specimens in comparison with those obtained from commercial human PSA ELISA kit. Importantly, Ti 3 C 2 MXenes-based interdigitated capacitance transducer open new opportunities for protein diagnostics and biosecurity.
研究领域:
生物传感器
·
分析化学
·
纳米技术
·
蛋白质诊断
·
生物安全
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Xiwen Jiang,Cuiyuan Pan,Qiaowen Wang,Xiao Han,Dianping Tang
Methods derived from photoelectrochemical (PEC) have been constructed for immunoassays , but most involve the split-type immunoreaction modes, and thus easily cause unpredictable intermediate precision. Herein, we innovatively designed an integrated PEC immunosensing platform for the quantitative monitoring of thyroglobulin (TG) on the gold nanoparticles (AuNPs)-functionalized BiVO 4 photoanode coupling with enzymatic biocatalytic precipitation (EBCP). This sensing system could simultaneously implement the immunoreaction and photocurrent measurement. Anti-TG capture antibodies were modified onto AuNPs-decorated BiVO 4 photoelectrode . A sandwich-type immunoreaction was carried out in the presence of target TG using horseradish peroxidase (HRP)-conjugated anti-TG detection antibody. The carried HRP molecules catalyzed 4-chloro-1-naphthol (4-CN) to generate an insoluble benzo-4-chlorohexadienone product on the photoanode in the presence of peroxide hydrogen, thereby decreasing the photocurrent. Under optimal conditions, the PEC immunosensors gave good photocurrent responses toward target TG within the dynamic range of 0.01–10 ng mL −1 at a detection limit of 7.6 pg mL −1 . Good repeatability and precision, high specificity and acceptable storage stability were acquired during the measurement. No significant differences were encountered for screening 15 human serum specimens between the developed PEC immunoassay and commercially available enzyme-linked immunosorbent assay (ELISA) method for the detection of target TG. Significantly, PEC immunosensing system offers promise for simple and cost-effective analysis of disease-related biomarkers.
研究领域:
分析化学
·
生物化学
·
材料科学
·
医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2026
·
IF 6.1
Qinyan Chen,Qiong Lin,Yibin Zeng,Haining Li,Xiao Han,Dianping Tang,Gaoda Ju
Background The design of a fully paper-based photoelectrochemical sensing platform holds significant potential for comprehensive community cancer screening. Results A portable photoelectrochemical (PEC) immunoassay was constructed based on TiO 2 hollow cube (HTCs) homojunctions with efficient photoelectric conversion properties and a paper-based printed three-electrode system. The sensor leveraged the specific immune recognition of carbohydrate antigen 19-9 (CA19-9) to mediate horseradish peroxidase (HRP)-catalyzed conversion of 4-chloronaphthol (4-CN) into insoluble, low-conductivity 4-chloronaphthol dimer (4-CD), inducing characteristic photocurrent attenuation. Under flashlight excitation, signals were recorded using a portable nanoampere meter. The sensor exhibited a wide linear range of 0.001–50 U mL −1 for CA19-9 and an ultra-low detection limit of 0.37 mU mL −1 . Furthermore, recovery tests on clinical serum samples from potential cancer patients confirmed good accuracy, and t -test results showed no significant difference between the PEC method and clinical ELISA kits, accompanied by excellent anti-interference performance. Significance This study provides a highly sensitive and portable detection platform for CA19-9, laying a foundation for its point-of-care and home-based early screening.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Environmental Sciences
·
2026
·
IF 6.3
Lihui Zhao,Can Jin,He Liu,Zhaozhe Yang,Yupeng Liu
Bisphenol A (BPA), a widespread endocrine-disrupting compound, necessitates efficient activation of peroxymonosulfate (PMS) for its degradation. Conventional carbon-based catalysts frequently exhibit limited efficiency, poor stability, and potential environmental risks. Bamboo-derived porous carbon material (BCM) and bamboo-derived porous carbon material loaded with Fe (BCM-Fe) serve as effective catalysts for activating PMS to degrade BPA. The BCM/PMS system achieved a BPA removal efficiency of 94.44 % within 150 minutes, while the BCM-Fe/PMS system attained 97.8 %. Quenching experiments and electron paramagnetic resonance (EPR) analysis identified non-radical singlet oxygen (¹O₂) as the dominant reactive species. Density functional theory (DFT) calculations demonstrated that atomically dispersed Fe species on BCM-Fe significantly enhanced PMS adsorption energy and activation kinetics compared to BCM. Both catalysts exhibited excellent reusability, confirming their potential as practical and sustainable catalysts. This study provides fundamental insights for designing efficient, environmentally benign carbon-based catalysts for advanced oxidation processes.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）