Scientific Reports
·
2020
·Vol. 10
·期 1
·1-9
·
IF 3.998
Zhang Lei,Weng Yi-jin,Liu Xiao,Gu Wen,Zhang Xia,Han Lei
Surface Enhanced Raman Scattering (SERS) has been widely applied in many research fields such as biological detection and chemical analysis. However, for the common Au nanoparticles, it’s too hard to guarante the three aspects: the great enhanced effect, the controllable aggregation and the uniformity of nanoparticles, the environmental friendliness and biocompatibility of nanoparticles. In this paper, phytate acid (IP6)-coated Au nanoparticles (IP6@Au NPs) are more stable and have a higher enhancement factor than Au nanoparticles. In order to achieve the uniformity of the spherical IP6-coated@Au nanoparticles (IP6@Au NPs), IP6 was used as a soft template. In the presence of IP6, IP6@Ag nanoparticles were first synthesized by reducing AgNO 3 with trisodium citrate, then IP6@Au NPs were obtained by reducing HAuCl 4 with Ag nanoparticles. The IP6@Au NPs exhibit excellent Raman signal enhancement by using p-aminothiophenol (4-ATP) as the probe molecules. The effects of Fe 3+ on the performance of IP6@Au NPs SERS substrates were also studied. The results show that SERS has the best enhancement effect when adding proper amount of Fe 3+ (0.56 PPM), and the limit of detection was 10 −7 M 4-ATP.
研究领域:
纳米技术
·
分析化学
·
材料科学
·
环境科学
✓ 已验证
DOI
PubMed
高影响因子
Journal of Materials Chemistry C
·
2023
·Vol. 11
·期 30
·10333-10340
·
IF 6.4
Cuixia Bi,Zhixiu Wang,Hongyan Zhao,Guangqiang Liu
The anisotropic morphology of metal nanomaterials with a rough surface is well-known to play an important role in surface-enhanced Raman scattering (SERS) and catalysis. In this work, a facile synthesis of multibranched Au nanostars with controllable shapes and tunable sizes was achieved in the presence of cysteine via a seed-mediated growth method. The effects of reaction parameters (such as cysteine, seed, gold precursor, AA and surfactant concentrations) on product morphology and size were systematically investigated by tracking the growth process and recording the morphological evolution process. Meanwhile, using 4-ATP as a Raman reporter, the Au nanostar substrate exhibits superior SERS characteristics, and can effectively detect the 4-ATP analyte at concentrations as low as 1 × 10−11 M. Moreover, the SERS substrate exhibits good Raman signal uniformity and reproducibility. This study opens up a new avenue for the synthesis of multibranched Au nanostructures, enabling promising applications in the fields of SERS detection, sensing and imaging.
研究领域:
纳米材料
·
分析化学
·
催化科学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Zelong Li,Xue Han,Lan Fu,Guochao Shi,Shiqi Xu,Mingli Wang,Wenzhi Yuan,Wenying Zhou,Jiahao Cui
The combination of surface-enhanced Raman scattering (SERS) and machine learning algorithm provides an effective means for the identification of pharmacodynamic substances. This paper reported a biomimetic synthesis route of Ag-30/SiO 2 -4/Cu-20/rice leaf (Ag-30/SiO 2 -4/Cu-20/RL) SERS platform with multiple synergistic electromagnetic enhanced performance. The localized surface plasmon resonance (LSPR) effect was strengthened as the SiO 2 nanolayer generated between Ag and Cu. This SERS platform demonstrated high sensitivity, with a low limit of detection (LOD) of 1 × 10 −10 M for 4-Aminophenylthiophenol (4-ATP) and an enhancement factor (EF) of 3.86 × 10 6 . More importantly, the principal component analysis (PCA) was adopted to analyze the SERS data of three different Traditional Chinese medicine (TCM) pharmacodynamic substances (Orientin, Atractylenolide III and Prim-o-glucosylcimifugin). The K-nearest neighbor (KNN) and Naïve Bayes (NB) achieved classification accuracy of 0.9474 and 0.9649, respectively. The platform provides guidance for the accurate identification of TCM pharmacodynamic substances.
研究领域:
分析化学
·
生物医学工程
·
中医药学
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产品关联
DOI
高影响因子
NANOTECHNOLOGY
·
2022
·Vol. 33
·期 40
·405501
·
IF 3.953
Tianxun Gong,Haonan Li,Guilin Wang,Fang Guan,Wen Huang,Xiaosheng Zhang
We propose an anti-scratch flexible surface-enhanced Raman scattering substrate with arrayed nanocavity microstructures fabricated by colloidal lithography. The nanocavity microstructure of the substrate can well protect the inner gold nanoparticles during wipe sampling. The prepared flexible substrate was able to detect 4-aminothiophenol (4-ATP) with a concentration down to 1 fM. Furthermore, the substrate was used to detect 6-BA residues on the surface of apples and bean sprouts through wipe sampling, which shows great potential in the field of rapid on-site detection, especially in the detection of pesticide residues on the surface of fruits and vegetables.
研究领域:
材料科学
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分析化学
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
Ting Zhou,Min Fan,Ruiyun You,Yudong Lu,Luqiang Huang,Yunchao Xu,Shangyuan Feng,Yang Wu,Huiying Shen,Lanjin Zhu
We have successfully prepared a highly sensitive sandwich nanosensor combined Fe 3 O 4 and [email protected] @Ag nanorods for histamine detection based on surface-enhanced Raman spectroscopy (SERS). The Fe 3 O 4 beads with –COOH served as a capture part to enrich histamine. The [email protected] @Ag core-shell nanorods functionalized with Nalpha,Nalpha-Bis(carboxymethyl)- l -lysine (AB-NTA) were then used to connect with the imidazolyl group of histamine, simultaneously the internal standard 4-aminothiophenol (4-ATP) in the core-shell structure was used as the SERS signal. PLS regression model based on concentration range 10 −3 -10 −8 mol/L showed a linear trend with R 2 = 0.9907. Our new approach can quickly and reliably determine histamine in fish sample and RAW264.7 cell lysates. This protocol for histamine extraction and SERS analysis enables the development of ultra-sensitive method for histamine detection.
研究领域:
分析化学
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纳米技术
·
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2019
·
IF 5.399
Bingyong Lin,Palanisamy Kannan,Bin Qiu,Zhenyu Lin,Longhua Guo
Simple, rapid, convenient, and economical surface enhanced Raman scattering (SERS) substrate is developed for on-site evaluation of Aflatoxin B 1 (AFB 1 ) in food matrix using handheld Raman Spectrometer. Self-assembly of gold nanobipyramids (Au NBPs) into the nanoholes of anodic aluminum oxide (AAO) template/pattern using ‘drop-dry’ approach provides a reliable pathway for the rapid fabrication of highly active and uniform SERS substrate. It shows enhanced and reproducible SERS signals towards the probe molecule, 4-aminothiophenol (4-ATP) with a relative standard deviation (RSD) of less than 10% and an average enhancement factor (EF) of 1 × 10 8 . For practical application, the proposed method is demonstrated for the detection of aflatoxin B 1 (AFB 1 ) in peanut extracts. The results show that the AFB 1 in peanut extracts can be identified within 1 min, with a limit of detection of 0.5 μg/L. Compared with conventional ELISA based AFB 1 analysis, our method is much more efficient (1 min versus >30 min).
研究领域:
分析化学
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食品安全
·
纳米技术
·
光谱学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Xia Yan,Hang Zhao,Hongyan Song,Jun Ma,Xiaofeng Shi
Surface-enhanced Raman scattering (SERS) has great potential for the detection of marine pollutants, but it is still restricted in ultra-trace and quantitative analysis. Here, a strategy for the detection of polycyclic aromatic hydrocarbons (PAHs) was proposed based on Au nanoscale convex polyhedrons (Au NCPs) coated with high-energy facets and embedded with 4-ATP as a probe molecule. Au NCPs acted as SERS substrates and led to limits of detection (LODs) for six common PAHs that reached 0.01 nM. Using internal calibration, the relative standard deviations (RSD) of the spectral stability and reproducibility were as low as 3.36% and 5.11%, respectively. The maximum mean relative errors (AREs) of the predicted and true values were 6.28%. The results indicate that the resulting Au NCPs improved the ultra-trace and quantitative detection of SERS, thus suggesting that the Au NCPs have practical application value in SERS.
研究领域:
环境科学
·
分析化学
·
纳米技术
·
材料科学
✓ 已验证
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Lixin Ma,Qian Xu,Limei Yin,Caixia Zou,Wei Wu,Chen Wang,Ruiyun Zhou,Zhiming Guo,Jianrong Cai
Fruits are susceptible to fungal infection and propagation during storage and transportation, and carry toxic metabolites of fungi. Patulin (PAT) is a primary mycotoxin contaminant in fruits and their products, with neurotoxicity, teratogenicity and carcinogenicity. The current chromatographic methods have limitations such as time-consuming, high-cost and the need for professional training. For rapid and sensitive detection of PAT, a surface-enhanced Raman scattering (SERS) method based on nucleophilic addition reaction was described in this paper. 4-aminothiophenol (4-ATP), silver ion, and cysteine (Cys) were successively attached to the surface of the Au-core-Ag-shell nanoparticles to form SERS sensors. PAT reacted with Cys to form a complex covering the surface of the sensor, attenuating the Raman signal from 4-ATP. Combined with the chemometrics algorithms, the hidden spectral information can be extracted effectively. Among these algorithms, the ant colony optimization partial least squares (ACO-PLS) model demonstrated the best predictive performance (R c = 0.9985, RMSEC = 0.0494). The recovery rate of PAT was 96.58 ∼ 101.74 % with the RSD ≤ 1.42 %. HPLC was used to verify the applicability of this SERS method, and the results indicated that the SERS method was consistent with the HPLC method ( p = 0.283 > 0.05). Furthermore, the SERS sensor was not subject to interference from ochratoxins A, alternariol, and the PAT structural analogues. Consequently, the proposed SERS sensor can realize rapid and highly selective detection of PAT, providing a new strategy for the rational design of SERS sensor based on chemical reaction for indirect detection of harmful substances.
研究领域:
分析化学
·
食品安全
·
光谱学
·
纳米技术
·
毒理学
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2023
·
IF 12.6
Huanhuan Li,Wenhui Geng,Zhixiong Qi,Waqas Ahmad,Suleiman A. Haruna,Quansheng Chen
In this work, a stimuli-responsive SERS biosensor was fabricated for tetracycline (TTC) by “signal-on” strategy using (EDTA)-driven polyethyleneimine grafted calcium carbonate ( [email protected] 3 ) microcapsule and chitosan-Fe magnetic microbeads ( [email protected] ). Initially, aptamer conjugated magnetic-bead [email protected] @Apt with superparamagnetism and excellent biocompatibility was employed as capture probe, which facilitated the rapid and easy magnetic separation. Subsequently, the PEI cross-linked layer and aptamer network layer were constructed onto the outer layer of CaCO 3 @4-ATP microcapsule to form sensing probes ( [email protected] 3 @ [email protected] ) via the layer-by-layer assembly method. In the presence of TTC, a sandwich SERS-assay was exploited by aptamer recognition induced target-bridged strategy. When the solution of EDTA was added, the core layer of CaCO 3 would be dissolved quickly, destroying the microcapsule to release 4-ATP. The released 4-ATP could be quantitatively monitored by dripping the supernatant onto the [email protected] SERS platform, resulting in a strong Raman “signal-on”. Under the optimal conditions, a good linear relationship was established with a correlation coefficient ( R 2 ) of 0.9938 and a LOD of 0.03 ng/mL. Additionally, the application capacity of the biosensor to detect TTC was also affirmed in food matrixes, and the results were consistent with the standard ELISA method ( P > 0.05). Hence, this SERS biosensor affords extensive application prospects for TTC detection with multiple merits such as high sensitivity, environment friendliness, and high stability.
研究领域:
分析化学
·
环境科学
·
食品安全
·
生物传感器
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）