SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Longfu Dai,Jian Liu,Da Li,Jiamei Hao,Hulin Gao
The hydroxamic acid reagents are extremely pervasive in the collector of scheelite flotation. The high flotation cost is caused by its large dosage and high price. A novel auxiliary collector 4-methodybenzoicacid (4-MBA) was used in combination with benzohydroxamic acid (BHA) to optimize the flotation reagents system for scheelite in the study. A single 4-MBA has weak collection capacity for scheelite. Nevertheless, the combination of 4-MBA and BHA optimized the flotation system of scheelite by ensuring its recovery and reducing the reagent consumption. The results of solution surface tension and contact angle measurements showed that the surface tension of the mixed reagent solution is a relatively lower and the hydrophobicity of the scheelite surface is a relatively higher in the BHA and 4-MBA mixed reagent system. Additionally, a synergistic effect between BHA and 4-MBA on Pb 2+ activated scheelite is revealed by the Zeta potential measurements, infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS). The addition of 4-MBA promoted the physical and chemical adsorption of BHA. The results of molecular dynamics simulation (MDS) further explained that the collector adsorption layer of the surface of scheelite (1 1 2) is more compact under BHA and 4-MBA mixed reagent system, which prevents the vertical entry of water phase and improves the hydrophobicity of scheelite. Finally, the bench-scale flotation experiment verified that the addition of 4-MBA enhanced scheelite flotation with BHA, and decrease the flotation cost of scheelite.
研究领域:
矿物加工工程
·
表面化学
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2026
·
IF 8.3
Meng Luo,Hongrui Huang,Xiao Liu,Danheng Gao,Qiegen Liu,Jin Li,Lianfu Li,Shichuan Xi,Yu Shi,Yuanyuan Song,Shuxuan Li
Novel integrated optofluidic in-fiber SERS probe based on 4-MBA/Ag NPs for high-stability, label-free detection of PYO. • Ultrafast PYO fingerprint identification within 5 s via a competitive coordination recognition strategy. • Quantitative evaluation of PAHs-triggered bacterial virulence enhancement, providing reliable technical support for marine environmental risk assessment and coastal public health early warning. The activation of virulence in Pseudomonas aeruginosa under environmental stress is of great significance for studying the mechanism of bacterial-environment interaction. Pyocyanin (PYO), a key phenazine virulence factor secreted by P. aeruginosa , serves as a direct indicator of bacterial pathogenicity. Herein, we developed a highly integrated optofluidic in-fiber surface-enhanced Raman scattering (SERS) biosensor for rapid, label-free detection of PYO secretion by Pseudomonas aeruginosa under environmental stress. 4-Mercaptobenzoic acid (4-MBA) serves as a molecular recognition linker anchored onto silver nanoparticle (Ag NPs) surfaces, which can undergo competitive coordination with pyocyanin to suppress non-specific interference from coexisting impurities. The nitrogen atoms on the phenazine ring of PYO form Ag–N coordination bonds along with π–metal coupling interactions with Ag NPs. This competitive substitution displaces the Ag–S bonds between 4-MBA and Ag NPs, leading to a concentration-dependent reduction in the characteristic peak intensity of 4-MBA at 1077 cm⁻¹ . The sensor specifically identifies PYO via its characteristic peak at 1345 cm⁻¹ within 5 s, and exhibits a linear range of 10 −5 M to 10 −14 M. The limit of detection (LOD) was calculated as 1.0 × 10⁻ 14 M. Through the sampling tests, we confirm that 50 μM PAHs stress triggers a 1.5-fold increase in PYO secretion of P. aeruginosa , verifying PAHs-induced bacterial virulence enhancement. Benefiting from the specific competitive recognition between 4-MBA and PYO, the sensor exhibits robust anti-interference performance in complex natural seawater matrices. This work provides a sensitive, rapid, and cost-effective SERS platform for bacterial virulence monitoring, offering valuable insights into pollutant-bacterium interactions and public health early warning. Download: Download high-res image (164KB) Download: Download full-size image
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高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2021
·
IF 4.098
Kaiqiang Wang,Jinjie Li
The fabrication of sensitive and reliable interfacial plasmonic platform for measuring chemical contaminants in various phases is an exciting topic in the food industry and for environment monitoring. In this study, a high-performance surface-enhanced Raman spectroscopy (SERS) analytic platform was developed through self-assembly of the gold@4-mercaptobenzoic acid@silver nanoparticles (Au@4-MBA@Ag NPs) at the cyclohexane/water interface. By addition of the inducer ethanol, the Au@4-MBA@Ag NPs in aqueous phase was effectively migrated to the biphasic interface, forming a large-scale close-packed nanoparticle array. The average gap between adjacent nanoparticles was smaller than 3 nm, where intensive SERS “hot spots” were created for high-sensitive detection. Furthermore, using the sandwiched 4-MBA molecule as the internal standard to correct the Raman signal fluctuations, the point-to-point and batch-to-batch reproducibility of Au@4-MBA@Ag array were improved with lower relative standard deviation (RSD) values of 8.84% and 14.97%, respectively, and pesticides (thiram and thiabendazole) analysis in both aqueous and organic phases were achieved with higher accuracy (R 2 of 0.986 and 0.990) as compared with those without 4-MBA correction (R 2 of 0.867 and 0.974). The high-throughput fabrication of the self-assembled nanoparticle array is a promising approach for development of a sensitive and reliable SERS platform for chemical contaminants monitoring in multiphase.
研究领域:
食品科学
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环境监测
·
纳米技术
·
分析化学
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Shijie Liu,Qian Ding,Lijun Zhao,Yueyv Bai,Miaoyun Li,Jong-Hoon Lee,Yaodi Zhu,Yijing Ding,Lingxia Sun,Yanxia Liu,Yangyang Ma,Dong Liang
In order to realize rapid identification of different species of lactic acid bacteria and rapid quantitative detection of mixed lactic acid bacteria. In this study, L. plantarum Y1 and P. pentosaceus Z1 were used as research subjects, and AuNPs@4-MBA was prepared as the SERS substrate material for the acquisition of SERS spectra of different species of lactic acid bacteria and the construction of taxonomic identification models and SERS digitized quantitative equations. The results showed that the enhancement factor of AuNPs to 4-MBA was 3.08 × 10 2 , and the reproducibility was excellent ( RSD < 10 %). With AuNPs@4-MBA as the base material, the differences of SERS peaks between L. plantarum Y1 and P. pentosaceus Z1 could be clearly distinguished. Principal component analysis (PCA) and cluster analysis (LDA) could completely visually classify and identify different species of lactic acid bacteria. The SERS quantitative equation y = 7509.32×-5942.69, R 2 = 0.99 and the detection limit lower than 10 CFU/mL was determined by the construction of SERS detection and quantitative model for different ratios and concentrations of mixed lactic acid bacteria. In the complex environment matrix, the detection accuracy of mixed lactic acid bacteria by this method reached 96.34 % to 103.54 %. It provides a promising technical strategy for rapid qualitative and real-time quantitative monitoring of mixed lactic acid bacteria.
研究领域:
分析化学
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纳米技术
·
微生物学
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光谱学
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食品科学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2025
·
IF 3.8
Song Xinyi,He Zhaoyuan,Tan Siyuan,Cong Siyu,Lu Wuli,Mai Zedeng,Jiang Mingsheng,Wen Yu,Liu Cheng,Chen Hailan
Deoxynivalenol (DON), a highly stable and globally prevalent mycotoxin, has become a critical food safety concern due to its widespread contamination in animal feed, particularly in corn, wheat, and other cereal-based ingredients. As one of the most frequently detected mycotoxins in feedstuffs, DON contamination not only causes significant economic losses in livestock production but also poses severe health risks to animals, including vomiting, immunosuppression, and growth retardation, which may ultimately affect human health through the food chain. Existing detection methods for DON are often limited by time-consuming procedures or analytical complexity. Here, we report a novel surface-enhanced Raman scattering-based lateral flow immunoassay (SERS-LFIA) for rapid, sensitive, and specific detection of DON residues in animal feeds. The biosensor employs core-shell Au@4-MBA@Ag nanoparticles functionalized with 4-mercaptobenzoic acid (4-MBA) as a Raman reporter and DON-specific antibodies for target recognition. After systematic optimization, the SERS-LFIA platform achieved both qualitative and quantitative detection within 16 min, with an exceptionally low limit of detection (LOD) of 0.01 µg/kg. The assay demonstrated excellent specificity, showing no cross-reactivity with other major mycotoxins, including aflatoxin B 1 , zearalenone, and ochratoxin A. Remarkable reproducibility was evidenced by intra- and inter-batch coefficients of variation (CV) of 3.51% and 2.19%, respectively. The test strips maintained stability for at least 3 months at 4 ± 0.5 °C and for 2 months at 25 ± 0.5 °C, making them particularly suitable for routine monitoring in feed mills and farms. This innovative SERS-LFIA system represents a significant advancement in mycotoxin detection technology, offering a robust, field-deployable solution for monitoring DON contamination in animal feed, which is crucial for ensuring feed safety and animal health. Graphical
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PubMed
高影响因子
ACS Sensors
·
2020
·
IF 7.333
Xiao-Bin Huang,Shao-Hua Wu,Hao-Cheng Hu,Jian-Jun Sun
The “turn-on” mode surface-enhanced Raman scattering (SERS) aptasensor for ultrasensitive ochratoxin A (OTA) detection was developed based on the SERS “hot spots” of AuNanostar@4-MBA@Au core–shell nanostructures (AuNS@4-MBA@Au) and exonuclease III (Exo III)-assisted target cycle amplification strategy. Compared with conventional gold nanoparticles, AuNS@4-MBA@Au provides a much higher SERS enhancement factor because AuNS exhibits a larger surface roughness and the lightning rod effect, as well as an excellent electromagnetic field between the AuNS core and the Au shell, which contribute to the superstrong SERS signal. Meanwhile, Exo III-assisted target cycle amplification can be used as an effective method for the further amplified detection of OTA. Additionally, the utilization of streptavidin magnesphere paramagnetic particles offers a green, economical, and facile technology for the accumulation and separation of the signal probe AuNS@4-MBA@Au from solution. All these factors lead to a significant enhancement of detectable signals and superhigh sensitivity. As a result, the limit of detection as low as 0.25 fg mL–1 could be achieved, which was lower than that in the other reported literatures on SERS methods for OTA detection as we know. The developed SERS aptasensor also provides a promising tool for foodstuff detection.
研究领域:
食品安全检测
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分析化学
·
纳米生物技术
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DOI
PubMed
高影响因子
APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING
·
2018
·Vol. 124
·期 12
·1-9
·
IF 1.604
Lin Shuang,Lin Xiang,Zhao Haiyan,Hasi Wuliji,Wang Li
Quantitative analysis always encounters difficulty in the field of surface-enhanced Raman spectroscopy (SERS) because of the lack of accurate and reliable analytical means. In this work, a new approach was demonstrated for the quantitative SERS measurement by self-assembled superlattice based on ultra-smooth Au nanospheres (NSs) embedded with 4-methylthiobenzoic acid (4-MBA) as the internal reference. Malachite green (MG) in water and chrysoidine in drinks with detection limit of 0.35 µM were successfully and quantitatively measured by employing Au@4-MBA@Au NSs superlattice as solid SERS substrate. Compared with conventional SERS methods, the fitting linearity of the relative SERS intensity vs. analyte concentrations has significantly improved by this new analysis means. As a result, our approach is a reliable analytical strategy to solve quantitative problems in SERS analysis, which can be easily applied for on-site detection and for preliminary monitoring of food samples.
研究领域:
分析化学
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纳米科学与技术
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食品安全检测
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Shan Xu,Peifang Chen,Xichi Lin,Imran Mahmood Khan,Xiaoyuan Ma,Zhouping Wang
Histamine is a significant biomarker to assess the freshness of fish products. In this study, a novel MOF-based SERS sensor for histamine determination was synthesized by wrapping PVP-capped Au nanoflowers with a ZIF-67 shell (Au [email protected] ). The highly branched Au NFs core exhibited a strong electromagnetic field enhancement effect and provided an ultra-sensitive SERS fingerprint spectrum, while ZIF-67 shell was the contributor to enrich the target and stabilize the substrate. The morphology of the core-shell structures can be easily controlled by the concentrations of the capping agent PVP and MOF precursor Co ion . Consequently, 4-MBA pre-grafted on the optimized SERS substrate can act as the Raman internal standard (IS) to eliminate signal fluctuations through standardizing all spectra against its peak at 1074 cm −1 . Moreover, as the specific receptor for histamine molecules, 4-MBA helped reach the low detection sensitivity, where the SERS intensity ratio, I 1172 /I 1074 presented a good linear relationship towards the histamine concentrations (10 −3 -10 −7 M) with the LOD of 0.87 × 10 −7 M (R 2 = 0.9930). Furthermore, the application in monitoring fish spoilage process demonstrated the feasibility and reliability of the developed sensor. This work provided a facile strategy to construct MOF-based SERS substrate as a potential platform for the shelf-life prediction of fish products.
研究领域:
材料科学
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分析化学
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食品科学
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DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Yu Lu,Xiufang Mo,Geng Zhu,Yan Huang,Yingchao Wang,Zhenzhong Yang,Liqiong Gao,Guofang Shen,Yi Wang,Xiangwei Zhao
Practical gas sensing application requires sensors to quantify target analytes with high sensitivity and reproducibility. However, conventional surface enhanced Raman scattering (SERS) sensor lacks reproducibility and quantification arising from variations of "hot spot" distribution and measurement conditions. Here, a ratio-dependent SERS sensor was developed for quantitative label-free gas sensing. Au@Ag-Au nanoparticles (NPs) were filtered onto anodic aluminum oxide (AAO) forming Au@Ag-Au@AAO SERS substrate. 4-MBA was encapsulated in the gap of Au@Ag-Au and served as the internal standard (IS) to calibrate SERS signal fluctuation for improved quantification ability. Combined with headspace sampling method, SO 2 residue in traditional Chinese medicine (TCM) can be extracted and captured on the immediate vicinity of Au@Ag-Au surface. The intensity ratio I 613 cm −1 /I 1078 cm −1 showed excellent linearity within the range of 0.5 mg/kg-500 mg/kg, demonstrating superior quantification performance for SO 2 detection. Signals for concentration as low as 0.05 mg/kg of SO 2 could be effectively collected, much lower than the strictest limit 10 mg/kg in Chinese Pharmacopoeia. Combined with a handheld Raman spectrometer, handy and quantitative TCM quality evaluation in aspect of SO 2 residue was realized. This ratiometric SERS sensor functioned well in rapid on-site SO 2 quantification, exhibiting excellent sensitivity and simple operability.
研究领域:
分析化学
·
材料科学
·
环境科学
·
药学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Xinran Yang,Zonglin Li,Junhui Du,Xueli Zhang,Haibin Liu,Xuechao Zhang,Lijun Li,Chuanjin Cui,Hongshuo Chen
In this paper, a SERS/electrochemical dual-channel aptamer biosensor was established for ultrasensitive detection of Pb 2+ . Firstly, the capture probe Fe 3 O 4 @Au@PAMAM@Aptamer and the signal probe AuNs@4-MBA@Au@ssDNA were chemically synthesized step by step. Secondly, the carboxyl graphene oxide (GO) was electrodeposited onto the electrode surface to gain the GO/SPCE. Meanwhile, PAMAM@Au nanomaterials were successfully synthesized and thus prepared the Aptamer/PAMAM@Au/GO/SPCE. During the process, SEM, TEM, FTIR and XPS were applied to characterize the synthesized nanomaterials which confirmed the successful synthesis of the nanomaterials. Finaly, the performance of this biosensor was evaluated in a series of Pb 2+ aqueous solutions by surface enhanced Raman (SERS) and differential pulse voltammetry (DPV). The results showed that the lowest limit of detection (LOD) was 3.16 × 10 −5 nM, and the sample recovery was 99.50–103.67 %. Furthermore, the biosensor demonstrated good specificity, repeatability, and stability. Reliable results were also obtained in actual sample testing. Therefore, it can provide a viable method for trace detection of heavy metal ions.
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高影响因子
APPLIED SPECTROSCOPY
·
2025
·
IF 2.2
XingYi Guo,Chun Zhu,Guoqing Chen,Lei Li,Chaoqun Ma,Taiqun Yang,Anqi Hu,WenHui Yang,YaLi Qian
In this paper, a lanthanide complex-based fluorescent sensor Tb(4-MBA) was developed for the selective recognition of diabetic ketoacidosis (DKA) and the diabetes biomarker β-hydroxybutyric (β-Hb). β-Hb significantly enhanced the fluorescence emission of the Tb(4-MBA) complex at 539 nm. Based on the analysis of the surface electrostatic potential distribution and time-resolved spectra, we speculate that in the reaction system of β-Hb with Tb(4-MBA), β-Hb and Tb(4-MBA) may form a complex through hydrogen bonding interactions, which brings β-Hb closer to Tb3+ and thus reduces the non-radiative energy loss of the solvent molecules to Tb3+ and significantly enhances the Tb(4-MBA) fluorescence intensity. The linear range of Tb(4-MBA) for β-Hb was 2–55 μM, and the limit of detection (LOD) was 50.6 nM. This sensor has high sensitivity and selectivity and shows great potential in the field of screening and diagnosis of diabetes mellitus and DKA.
研究领域:
分析化学
·
生物无机化学
·
荧光传感
·
糖尿病研究
·
材料科学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Yumin Chen,Weiwei Cheng,Yuling Yang,Di Wu,Yan Zhang,Xiaozhi Tang
A versatile Surface-enhanced Raman Spectroscopy (SERS) aptasensor was fabricated for ultrasensitive and stable aflatoxin B1 (AFB1) detection using complementary DNA strands (cDNA) modified Fe 3 O 4 @UiO-66-NH 2 as capture probe and aptamer modified Au@ 4-MBA@AgNPs as signal probe . The surface of the Fe 3 O 4 beads was functionalized with UiO-66-NH 2 to improve the surface area and content of reactive amino groups for stable immobilization of cDNA and capture of more signal probes. Raman tag (4-mercaptobenzoic acid, 4-MBA) embedded Au@AgNPs nanoparticles (Au@4-MBA@AgNPs) was used as signal probes to achieve strong SERS intensity and protect the tag molecules from external interference. When no AFB1 existed, a strong SERS intensity of the signal probe was observed due to the DNA hybridization reaction between the capture and signal probe. While, the addition of AFB1 led to the dissociation of signal probes from the capture probes, leading to a decreased Raman intensity . As a result, a low limit of detection of 1.47 × 10 −7 ng/mL and wide linear range from 10 −6 to 10 2 ng/mL were obtained for determination of AFB1 based on the fabricated aptasensor. Moreover, an excellent selectivity and desirable recoveries from 94.3 % to 103.3 % in real maize samples were obtained, demonstrating the fabricated SERS aptasensor is promising in practical applications.
研究领域:
分析化学
·
食品安全
·
纳米技术
·
光谱学
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DOI
高影响因子
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