PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
·
2017
·
IF 1.361
Xiaoyan Li,Chen Qi,Peilian Wei,Lei Huang,Jin Cai,Zhinan Xu
Uridine 5′-diphosphate N-acetylglucosamine (UDP-GlcNAc) is a natural UDP-monosaccharide donor for bacterial glycosyltransferases, while uridine 5′-diphosphate N-trifluoacetyl glucosamine (UDP-GlcNTFA) is its synthetic mimic. The chemoenzymatic synthesis of UDP-GlcNAc and UDP-GlcNTFA was attempted by three recombinant enzymes. Recombinant N-acetylhexosamine 1-kinase was used to produce GlcNAc/GlcNTFA-1-phosphate from GlcNAc/GlcNTFA. N-acetylglucosamine-1-phosphate uridyltransferase from Escherichia coli K12 MG1655 was used to produce UDP-GlcNAc/GlcNTFA from GlcNAc/GlcNTFA-1-phosphate. Inorganic pyrophosphatase from E. coli K12 MG1655 was used to hydrolyze pyrophosphate to accelerate the reaction. The above enzymes were expressed in E. coli BL21 (DE3) and purified, respectively, and finally mixed in one-pot bioreactor. The effects of reaction conditions on the production of UDP-GlcNAc and UDP-GlcNTFA were characterized. To avoid the substrate inhibition effect on the production of UDP-GlcNAc and UDP-GlcNTFA, the reaction was performed with fed batch of substrate. Under the optimized conditions, high production of UDP-GlcNAc (59.51 g/L) and UDP-GlcNTFA (46.54 g/L) were achieved in this three-enzyme one-pot system. The present work is promising to develop an efficient scalable process for the supply of UDP-monosaccharide donors for oligosaccharide synthesis.
研究领域:
生物化学
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酶学
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合成生物学
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微生物学
✓ 已验证
DOI
PubMed
高影响因子
ACS Omega
·
2025
·
IF 4.3
Luna Wang,Yan Gong,Yanming Miao,Zhifeng Zhang
To support the development of a low-carbon economy and contribute to the achievement of carbon neutrality, this study synthesized bifunctional zeolitic imidazolate framework-8-based nanozymes (ZNZs) with dual functionality: CO2 adsorption and carbonic anhydrase (CA)-mimetic catalytic activity. The influence of ZNZs at varying concentrations on the growth rate of microalgae, chlorophyll and carbohydrate content, and ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and CA activity were explored. Results indicated that after the addition of 5 mg/L ZNZs, the growth rate of microalgae increased by 7%. Furthermore, the maximum chlorophyll and carbohydrate contents reached 4.55 and 43.59 mg/L, respectively, which were 12.6 and 4.2% higher than those in the control group. Additionally, Rubisco and CA activity (intra- and extracellular) increased by up to 21.26, 10, and 8.3%, respectively. These findings demonstrate that ZNZs enhance the photosynthetic efficiency of microalgae and increase microalgae’s affinity for CO2, highlighting their promise as a strategy for improving carbon capture and sustainable bioenergy production.
研究领域:
环境科学
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纳米技术
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生物技术
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生物化学
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气候变化缓解
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Chemistry-An Asian Journal
·
2015
·Vol. 10
·期 11
·2503-2511
·
IF 4.587
Chao Qi,Ying-Jie Zhu,Tuan-Wei Sun,Jin Wu,Feng Chen
Graphical Amorphous calcium phosphate mesoporous microspheres (ACP-MSs) have been synthesized using adenosine 5′-diphosphate disodium salt as an organic phosphorus source by a rapid microwave-assisted hydrothermal method. The ACP-MSs drug delivery system shows a pH-responsive drug release behavior and a high ability to damage cancer cells after loading doxorubicin. Herein we report a rapid and green strategy for the preparation of amorphous calcium phosphate mesoporous microspheres (ACP-MSs) using adenosine 5′-diphosphate disodium salt (ADP) as an organic phosphorus source by a microwave-assisted hydrothermal method. The effects of the pH value, the reaction time, and temperature on the crystal phase and morphology of the product are investigated. The ADP biomolecules used in this strategy play an important role in the formation of ACP-MSs. The as-prepared ACP-MSs are efficient for anticancer drug delivery by using doxorubicin (Dox) as a model drug, and the Dox-loaded ACP-MSs show a high ability to damage cancer cells. Moreover, the ACP-MSs drug delivery system exhibits a pH-responsive drug-release behavior due to the degradation of ACP-MSs at a low pH value, thus, it is promising for applications in pH-responsive drug delivery.
研究领域:
材料科学
·
生物医学工程
·
药学
✓ 已验证
DOI
高影响因子
Autophagy
·
2022
·
IF 13.391
Haitao Wang,Jianhua Zhang,Haoqiu Liu,Man Wang,Yan Dong,Yijun Zhou,Sek-Man Wong,Kai Xu,Qiufang Xu
Hosts can initiate macroautophagy/autophagy as an antiviral defense response, while viruses have developed multiple ways to evade the host autophagic degradation. However, little is known as to whether viruses can target lipids to subvert autophagic degradation. Here, we show that a low abundant signaling lipid, phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2), is required for rice black-streaked dwarf virus (RBSDV) to evade the autophagic degradation in the insect vector Laodelphax striatellus. RBSDV binds to PtdIns(3,5)P2 and elevates its level through its main capsid protein P10, leading to inhibited autophagy and promoted virus propagation. Furthermore, we show that PtdIns(3,5)P2 inhibits the autophagy pathway by preventing the fusion of autophagosomes and lysosomes through activation of Trpml (transient receptor potential cation channel, mucolipin), an effector of PtdIns(3,5)P2. These findings uncover a strategy whereby a plant virus hijacks PtdIns(3,5)P2 via its viral capsid protein to evade autophagic degradation and promote its survival in insects.
研究领域:
植物病毒学
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昆虫媒介生物学
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细胞生物学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Bioactive Materials
·
2022
·
IF 16.874
Yihao Liu,Haoyi Niu,Chengwei Wang,Xiaoxiao Yang,Wentao Li,Yuxin Zhang,Xiaojun Ma,Yuanjing Xu,Pengfei Zheng,Jinwu Wang,Kerong Dai
Uncontrolled hemorrhage and wound infection are crucial causes of trauma-associated death in both the military and the clinic. Therefore, developing an efficient and rapid hemostatic method with biocompatibility, easy degradation, and wound healing is of great importance and desirability. Inspired by spontaneous blood cell plug formation in the hemostasis process, an adenosine 5′-diphosphate modified pro-coagulation hyaluronic acid (HA-ADP) coordinated with enhanced antibacterial activity of undecanal-modified chitosan (UCS) was fabricated through physical electrostatic cross-linking and freeze-drying. The as-prepared hydrogel sponges showed a porous structure suitable for blood cell adhesion. In particular, the hydrogel exhibited excellent antibacterial ability and promoted the adhesion of platelets and red blood cells, thus inducing a prominent pro-coagulation ability via platelet activation, which exhibits a shorter hemostasis time (58.94% of control) in vitro . Compared with commercially available CELOX and gelatin sponge (GS), HA-ADP/UCS accelerates hemostasis and reduces blood loss in both rat tail amputation and rat artery injury models. Furthermore, all the samples exhibited superior cytocompatibility and biodegradability. Due to these performances, HA-ADP/UCS promoted full-thickness skin defect healing significantly in vivo . All the properties of HA-ADP/UCS suggest that it has great potential for translation as a clinical application material for hemostatic and wound healing.
研究领域:
生物材料
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止血
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伤口愈合
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生物医学工程
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2024
·Vol. 22
·期 1
·1-20
·
IF 10.6
Li Tan,Zhang Xingyu,Shi Chengyu,Liu Qiao,Zhao Yuetao
Combination of tumor immunotherapy with photothermal therapy (PTT) is a feasible tactic to overcome the drawback of immunotherapy such as poor immune response. Via triggering the immunogenic cells death (ICD), PTT can stimulate the activity of immune cells, but meanwhile, the level of adenosine is elevated via the CD73-induced decomposition of ATP which is overexpressed accompanying with the PTT process, resulting in negative feedback to impair the immune stimulation. Herein, we developed a novel biomimetic photothermal nanodrug to specifically block CD73 for inhibition of adenosine production and more efficient priming of the suppressive immune microenvironments. The nanodrug, named as AptEM@CBA, is constructed by encapsulation of photothermal agent black phosphorus quantum dots (BPQDs) and selective CD73 inhibitor α, β-Methyleneadenosine 5′-diphosphate (AMPCP) in chitosan nanogels, which are further covered with aptamer AS1411 modified erythrocyte membrane (EM) for biomimetic camouflage. With AS1411 induced active targeting and EM induced long blood circulation time, the enrichment of the nanodrug tumor sites is promoted. The photothermal treatment promotes the maturation of dendritic cells. Meanwhile, the release of AMPCP suppress the adenosine generation via CD73 blockade, alleviating the impairment of adenosine to dendritic cells and suppressing regulatory T cells, synergically stimulate the activity of T cells. The combination of CD73 blockade with PTT, not only suppresses the growth of primary implanted tumors, but also boosts strong antitumor immunity to inhibit the growth of distal tumors, providing good potential for tumor photoimmunotherapy.
研究领域:
纳米技术
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癌症免疫治疗
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光热疗法
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药物递送系统
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）