ACS Sensors
·
2026
·
IF 10.9
Shuai Liu,Zishan Ding,Xing Lu,Zhiyong Liu,Wenrui Ma,Hanqing Xu,Hengjun Zhang,Xianling Dai,Man Shen,Yingchun Huang,Mingxuan Gao,Jing Bao,Ming Chen
Rapid and sensitive detection of antibiotic-resistant bacteria (ARB) remains a critical challenge in clinical and public health settings. This study describes the successful construction of a portable DNA activator-triggered entropy-driven catalysis-modulated CRISPR/Cas12a-based sensor (PSDA) for the ultrasensitive and rapid detection of multiple ARB. This PSDA platform utilizes a CRISPR/Cas12a-mediated signal transduction strategy, in which a target-specific DNA activator initiates an entropy-driven dynamic DNA network for signal amplification. To further enhance detection performance, a 3D-printed microfluidic chip device with a smartphone-based readout system has been integrated into the sensor, using green-emitting Zn2GeO4:Mn persistent luminescent nanoparticles as a novel molecular beacon for fluorescence enhancement. This platform enables the simultaneous detection of methicillin-resistant Staphylococcus aureus, carbapenem-resistant Pseudomonas aeruginosa, and Klebsiella pneumoniae carbapenemase 2 (KPC-2)-expressing Klebsiella pneumoniae (KPC-2 KP) with a broad dynamic range (1−107 CFU/mL), an ultralow detection limit (1 CFU/mL), and rapid analysis (∼45 min). The assay results are also highly consistent with those of conventional plate counting methods (95.48−115.15%). Overall, this study presents a cost-effective, rapid-response biosensing platform for the simultaneous detection of multiple ARB, with direct applications in clinical diagnostics, food safety monitoring, and environmental surveillance.
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Communications Materials
·
2026
·
IF 9.1
Ma Wei,Zhang Jin-Yan,Wang Dun,Li Zhi-Cheng,Cheng Xiang-Yi,Chen Ling-Long,Liu Ying,Kang Gui-Ying,Yu Cui-Yun,Wei Hua
The leverage of synthetic polymer self-assemblies (SPSAs) as biomimetic allostery provides unique opportunities for developing bio-inspired soft species. However, the preparation of these soft species remains a challenge likely due to the synthetic difficulties toward SPSAs having four-level hierarchical structural transitions and the compromised colloidal stability of the self-assembled quaternary structures. Herein, we report self-assembly of topological polymers with hydrophilic multicycles (HMCs) for biomimetic allostery. Specifically, cationic copolymers containing HMCs are prepared via a reliable synthetic approach, whose hierarchical self-assembly affords stabilized nanoparticles that undergo biomimetic allostery into quaternary petalled self-assemblies at an intracellular acidic pH of 5.0. The quaternary petalled self-assemblies lead to a mechanical disruption effect on lysosomes like nanomachines, further resulting in enhanced drug delivery efficiency of an anticancer drug-loaded multicyclic copolymers with a tumor inhibition rate 1.6-fold greater than that of the free drug in a murine 4T1 model. Overall, our work highlights the use of HMCs as biomimetic allostery of SPSAs for enhanced biomedical applications.
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Frontiers in Bioengineering and Biotechnology
·
2023
·
IF 5.7
Wang Youwei,Chen Bing,Fan Jiang,Wang Zhong
Monitoring ferroptosis-related miRNAs is crucial for the treatment and prognosis of patients with intracerebral hemorrhage. In this work, a novel hydrophobic paper (h-paper)-based plasmonic substrate was produced by dropping DS Au nanorods with a narrow range of sizes and morphologies onto h-paper. Raman reporter molecules were adsorbed to the array surface, and surface-enhanced Raman scattering spectra at randomly selected points reveal uniform and significant SERS enhancement. Hairpin DNAs labelled with Raman reporters and hybridized with placeholder DNAs were decorated on SERS substrate to fabricate SERS biosensor. Target miRNAs initiated the “inverse Molecular Sentinel” process. During the process, PHs were removed and the conformation of HPs changed toward the hairpin structure, thus eliciting the proximity of Raman reporter to substrate and a stronger SERS signal. The proposed SERS biosensor performs well in terms of stability, reproducibility, and selectivity. The limits of detection of miR-122-5p and miR-140-5p in serum were 4.17 aM and 4.49 aM, respectively. Finally, the fabricated SERS biosensor was applied to detect miR-122-5p and miR-140-5p in ICH patients and healthy subjects, and the results obtained by SERS were consistent with the results from quantitative real-time polymerase chain reaction, revealing the accuracy of the method. This simple, rapid approach offers great potential for the simultaneous detection of miRNAs in practical clinical applications.
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Materials Chemistry Frontiers
·
2022
·Vol. 6
·期 19
·2869-2879
·
IF 8.683
Weizhou Sha,Mingzhu Wang,Wei Wang,Zhi Yuan
Fluorescence imaging has gained wide attention due to its high sensitivity and resolution and can be used to direct tumour resection. The contrast in image formation is an important factor in tumour fluorescence imaging. Therefore, designing a responsive fluorescence imaging material is crucial for guiding the surgical resection of tumours. Spirolactam rhodamine has shown great potential in cell imaging and ion detection due to its excellent acid-responsive fluorescence. Nevertheless, poor water solubility seriously confines its further application in living organisms. In this study, to improve both solubilization and tumour targeting, hyaluronic acid was modified with spirolactam rhodamine, self-assembling into nanoparticles (HA-RdB-EDS NPs) with a narrow diameter size distribution of 200 nm through hydrophilic and hydrophobic interactions. The results showed that HA-RdB-EDS NPs could be turned on in response to the low pH of the tumour microenvironment. The pKa value of the NPs was calculated to be about 5.5, suitable for the detection of tumours. Besides, the HA-RdB-EDS NPs have fluorescence turn-on/off reversibility between pH = 5.5 and 7.4. According to the results of cell fluorescence stability experiments, about 50% of the fluorescence intensity of HA-RdB-EDS NPs was maintained after 5 minutes of persistent excitation. Endocytosis experiments demonstrated that HepG2 cells showed greater selection for HA-RDB-EDS NPs than 3T3 cells, about 2.7 times. After the intravenous injection of NPs into tumour-bearing mice, fluorescence could be turned on at the tumour. All these results indicate that HA-RdB-EDS NPs have the potential for clinical use.
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NEW JOURNAL OF CHEMISTRY
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2022
·Vol. 46
·期 21
·10299-10307
·
IF 3.925
Xiao-Qin Yang,Li-Wen Bai,Yu Chen,Yue-Xiao Lin,Hua Xiang,Ting-Ting Xiang,Shuang-Xing Zhu,Li Zhou,Kai Li,Xinxiang Lei
Polypeptides provide unique possibilities for the development of efficient and selective fluorescent probes for different biomarkers because of their highly modular nature, the feasibility of chemical synthesis and fluorescence modification, and biomolecular recognition potential. Herein, a bacteriophage (phage) display was utilized to screen affinity peptides against dihydrofolate reductase. On this basis, positive peptide DHFR-Y1(NH2-WSLGYTG-COOH) was obtained utilizing solid-phase peptide synthesis. Furthermore, fluorescein FAM was used to chemically modify the N-terminus and C-terminus of the peptide, respectively, to generate N-terminal fluorescent peptide DHFR-Yn (Flu-βA-WSLGYTG-COOH) and C-terminal fluorescent peptide DHFR-Yc (NH2-WSLGYTL(Flu)-COOH). In the subsequent experiment, multiple in vitro detection methods (including DSF, STD-NMR, BLI) indicated that the binding ability of affinity peptide DHFR-Y1 (KD = 3.57 μM) is significant to DHFR, in contrast to the stronger noncovalent binding ability of C-terminal fluorescent peptide (KD = 0.56 μM) and weak affinity of N-terminal fluorescent peptide (KD = 14.70 μM). The evidence that DHFR-Y1 and DHFR-Yc bind to folic acid binding pocket of DHFR was coming up by the in silico study. The peptide DHFR-Yc is adequate as a fluorescent probe targeting dihydrofolate reductase.
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TALANTA
·
2023
·
IF 6.1
Xiaowei Cao,Zhengqing Liu,Xiaogang Qin,Yuexing Gu,Yong Huang,Yayun Qian,Zhenguang Wang,Hongbo Li,Qunshan Zhu,Wei Wei
Colorectal cancer (CRC) remains a significant contributor to the global mortality rate, and a single biomarker cannot meet the specificity required for CRC screening. To this end, we developed a multiplexed, pump-free surface-enhanced Raman scattering (SERS) microfluidic chip (LoC-SERS) using a one-step recognition release mechanism; the aptamer-functionalized novel Au nanocrown array (AuNCA) was used as the detection element embedded in the detection zone of the platform for rapid and specific detection of protein markers in multiple samples simultaneously. Here, the corresponding aptamer specifically captured the protein marker, causing the complementary strand of the aptamer carrying the Raman signal molecule to be shed, reducing the SERS signal. Based on this platform, sensitive and specific detection of the target can be accomplished within 15 min with detection limits of 0.031 pg/mL (hnRNP A1) and 0.057 pg/mL (S100P). Meanwhile, the platform was consistent with ELISA results when used to test clinical. By substituting different aptamers, this platform can provide a new solution for the rapid and sensitive detection of protein markers, which has promising applications in future disease detection.
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Advanced Science
·
2023
·
IF 15.1
Min Li,Honglang Lu,Menghan Pi,Hui Zhou,Yufei Wang,Bin Yan,Wei Cui,Rong Ran
The development of hydrogel-based underwater electronics has gained significant attention due to their flexibility and portability compared to conventional rigid devices. However, common hydrogels face challenges such as swelling and poor underwater adhesion, limiting their practicality in water environments. Here, a water-induced phase separation strategy to fabricate hydrogels with enhanced anti-swelling properties and underwater adhesion is presented. By leveraging the contrasting affinity of different polymer chains to water, a phase-separated structure with rich hydrophobic and dilute hydrophilic polymer phases is achieved. This dual-phase structure, meticulously characterized from the macroscopic to the nanoscale, confers the hydrogel network with augmented retractive elastic forces and facilitates efficient water drainage at the gel-substrate interface. As a result, the hydrogel exhibits remarkable swelling resistance and long-lasting adhesion to diverse substrates. Additionally, the integration of carboxylic multiwalled carbon nanotubes into the hydrogel system preserves its anti-swelling and adhesion properties while imparting superior conductivity. The conductive phase-separated hydrogel exhibited great potential in diverse underwater applications, including sensing, communication, and energy harvesting. This study elucidates a facile strategy for designing anti-swelling underwater adhesives by leveraging the ambient solvent effect, which is expected to offer some insights for the development of next-generation adhesive soft materials tailored for aqueous environments.
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Materials Science & Engineering C-Materials for Biological Applications
·
2021
·
IF 7.328
Zechun Dang,Jizheng Sun,Jiaqi Fan,Jinqi Li,Xinlei Li,Tongsheng Chen
Zinc oxide (ZnO) nanostructures have been widely studied in biomedical fields due to their special properties. In recent years, ZnO spherical nanoparticles (SNPs) with nano-size as an anti-tumor agent have been widely concerned. While the effects of the non-spherical shaped ZnO nanoparticles on tumor cell death have been rarely reported. Here, we prepared ZnO spiky nanoparticles (SPNPs) as the research subject. We found that the SPNPs showed superiority in killing tumor cells. To be specific, SPNPs presented a long-term cytotoxicity effect on killing tumor cells, as plenty of SPNPs retained on the cell plasma membrane's exterior and still showed toxicity effect on tumor cells after co-incubation multiple times. Moreover, compared to SNPs, it was encouraging that SPNPs still showed stronger cytotoxicity in both simulated circulatory systems of tumor cells and 3D tumor cell spheroids. The stronger toxicity against tumor cells suggested that ZnO SPNPs have more advantages on killing tumor cells as a promising nanomedicine.
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MICROPOROUS AND MESOPOROUS MATERIALS
·
2020
·
IF 4.551
Di Wang,Erjun Sun
In this paper, a sensing probe (denoted as CFE) for tumor-biomarker 5-hydroxyindoleacetic acid (5-HIAA) was firstly synthesized. Its sensing mechanism was confirmed as the recovery of fluorescein structure via an ester-exchange reaction, accompanied with emission turn on effect towards 5-HIAA. To improve photostability and minimize self-quenching/aggregation of CFE molecules, they were loaded into silica based MCM-41 spheres and UCNPs-cored (up-conversion nanoparticles) MCM-41 spheres. Corresponding composite samples, denoted as CFE@MCM-41 and CFE@MCM-41@UCNPs, were carefully discussed to confirm their structure, including XRD , N 2 adsorption and desorption, SEM/TEM and TGA . The energy transfer from secondary excitation source UCNPs to probe CFE was confirmed by Forester radius calculation and emission lifetime comparison. Sensing performance of CFE@MCM-41 and CFE@MCM-41@UCNPs were finally discussed in detail. They showed emission turn on effect towards 5-HIAA with good selectivity. CFE@MCM-41 showed linear response, while CFE@MCM-41@UCNPs showed exponential response, resulting in a warning signal when analyte concentration was higher than a critical level. The reason for this exponential response was discussed and explained. Their practical sensing result for human urine test was compared to HLPC method and literature methods, which confirmed the promising result of CFE@MCM-41 and CFE@MCM-41@UCNPs.
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Journal of Materials Chemistry B
·
2019
·Vol. 7
·期 48
·7676-7682
·
IF 5.047
Xiuxia Sun,Linyan Yao,Caihong Fu,Liting Luo,Jie Wang,Jianxi Xiao
Collagen with a single amino acid substitution is the main cause of a plethora of heritable disorders such as Osteogenesis Imperfecta and Ehlers-Danlos syndrome. Though significant advances have been achieved in the development of protein assays, it remains very challenging to distinguish a protein with a single amino acid mutation from the wild-type protein. A novel fluorescent self-quenching assay has been constructed to detect target collagen peptides with a single amino acid mutation using two probe peptides. The hybridization of the probe peptide and the natural target collagen peptide results in a complete heterotrimer and strong fluorescence, whereas the mixture of the probe peptide and the mutation collagen sequences leads to a partial homotrimer and pronounced fluorescence self-quenching. The extent of fluorescence quenching is dependent on the identity of the residue replacing Gly following the order of Ala < Ser < Arg, while the Gly–Ala mutation causes the mildest fluorescence loss. The probe peptide-based fluorescence self-quenching assay facilitates specific detection of the target collagen sequence with a single Gly mutation at the nM level. The simultaneous utilization of both probe peptides enables efficient discrimination between different mutation peptides. To our knowledge, our work may be the first report of a robust analytical assay that can identify collagen fragments with single amino acid mutation, which will greatly contribute to deciphering the molecular mechanism of Osteogenesis Imperfecta as well as developing novel diagnostic strategies.
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ADVANCED FUNCTIONAL MATERIALS
·
2015
·Vol. 26
·期 6
·872-880
·
IF 11.805
Kunyuan Luo,Yuhong Yang,Zhengzhong Shao
Developing hydrogel which combines superior mechanical performance and biocompatibility attracts researchers’ attention in recent years. Here, a novel biocompatible hydrogel with excellent mechanical performance, comprised of regenerated silk fibroin (RSF) and hydroxypropyl methyl cellulose (HPMC), is fabricated by simply mixing and heating. It is found that both of compressive modulus and tensile modulus of the optimal RSF/HPMC hydrogel are over 1.0 MPa. Meanwhile, the break energy is up to 3500 J m −2 , which is higher than that of some natural elastomers, such as cartilage, cork, and skin. The investigation of gelation mechanism reveals that more uniformly dispersed crosslinks dominated by smaller β-sheet structures, which is attributed to the synergistic effects of hydrogen bonding and hydrophobic interaction between HPMC and RSF molecules, contribute to the superior mechanical performance of RSF/HPMC hydrogel. This biocompatible high strength silk protein based hydrogel diversifies the robust hydrogels and holds a great promise as candidates for load-bearing materials in biomedical field.
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Journal of Materials Chemistry A
·
2024
·
IF 10.7
Ying Luo,Da Lei,Maohua Li,Yuansheng Ge,Jiguang Li,Baiyi Zu,Jun Yao,Xincun Dou
Metal organic frameworks (MOFs) have made great progress in the field of fluorescent sensing; however, whether the electronic structure and recognized sites can be adjusted by external functionalization still remains challenging. Here, by postmodifying 5-carboxyfluorescein (Flu), externally coupled orbitals were introduced into the electronic structure of UiO-66-OH, and thus an effective photo-induced energy transfer (PET) process was established in functionalized MOFs (UiO-66-Flu), resulting in the reduction of the intrinsic blue fluorescence. Due to the formation of the ester bond by effective incorporation of the surface –OH with Flu, the hydrazinolysis reaction triggered by hydrazine could happen and the Zr6O6 clusters could further reduce the reaction energy barrier through strong hydrogen bonding with hydrazine, resulting in a remarkably low limit of detection (LOD, 27.17 nM) and a fast response (4 s). Furthermore, with polyvinylidene fluoride (PVDF) as the dispersion medium, UiO-66-Flu was fabricated into a UiO-66-Flu/PVDF film sensor, enabling the rapid detection of gaseous hydrazine within 2 s in simulated practical scenarios. We expect that the present fluorescent ligand modifying concept will not only deepen the understanding of electronic structure engineering in functionalized MOF design, but also shine light on the exploration of high-performance MOF-based sensing strategies.
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