MICROPOROUS AND MESOPOROUS MATERIALS
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2020
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IF 4.551
Di Wang,Erjun Sun
In this paper, a sensing probe (denoted as CFE) for tumor-biomarker 5-hydroxyindoleacetic acid (5-HIAA) was firstly synthesized. Its sensing mechanism was confirmed as the recovery of fluorescein structure via an ester-exchange reaction, accompanied with emission turn on effect towards 5-HIAA. To improve photostability and minimize self-quenching/aggregation of CFE molecules, they were loaded into silica based MCM-41 spheres and UCNPs-cored (up-conversion nanoparticles) MCM-41 spheres. Corresponding composite samples, denoted as CFE@MCM-41 and CFE@MCM-41@UCNPs, were carefully discussed to confirm their structure, including XRD , N 2 adsorption and desorption, SEM/TEM and TGA . The energy transfer from secondary excitation source UCNPs to probe CFE was confirmed by Forester radius calculation and emission lifetime comparison. Sensing performance of CFE@MCM-41 and CFE@MCM-41@UCNPs were finally discussed in detail. They showed emission turn on effect towards 5-HIAA with good selectivity. CFE@MCM-41 showed linear response, while CFE@MCM-41@UCNPs showed exponential response, resulting in a warning signal when analyte concentration was higher than a critical level. The reason for this exponential response was discussed and explained. Their practical sensing result for human urine test was compared to HLPC method and literature methods, which confirmed the promising result of CFE@MCM-41 and CFE@MCM-41@UCNPs.
研究领域:
生物化学
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医学诊断
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
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2025
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IF 5.1
Yuying Han,Xingyu Hou,Yali Li,Xiaopeng Yang,Xue Sun,Xinxin Bian,Na Sun,Yuguang Lv,Lixin Ding,Ze Li
This study utilized Panax notoginseng leaves carbon dots (PN-CDs) with red and blue fluorescence to detect 5-hydroxyindoleacetic acid (5-HIAA). It is a straightforward one-pot hydrothermal technique using Panax ginseng leaves to synthesize these CDs. Under 400 nm excitation, 5-HIAA decreases the fluorescence intensity at 670 nm while enhancing the fluorescence intensity at 420 nm. The fluorescence intensity ratio (F 670 /F 420 ) exhibited a significant linear relationship over the 5-HIAA concentration range of 0–100 μM. Notably, smartphones could capture the observable color change of the PN-CDs under 365 nm UV exposure and perform RGB (red/green/blue) analysis for color quantification using the smartphone’s software. The detection limit for its fluorescence detection was determined to be 0.023 μM, whereas that of mobile phone detection was 0.36 μM. This study proposes a novel platform combining fluorescence sensing and cell phone technology, facilitating real-time analysis of 5-HIAA levels in human urine.
研究领域:
生物化学
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医学检测
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
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2025
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IF 6.7
Xin Tong,Zicheng Wang,Lina Zhao,Kai Wang,Yuxin Li
Lanthanide coordination compounds (LnCC), as excellent luminophores, exhibit ultrasensitive responses to the structural details of coordination environments, providing an effective platform for chemical and biomedical detection. Due to the overtones of molecular vibrations in water that cause luminescence quenching, LnCC-based sensors are typically used in heterogeneous systems, where the compounds are dispersed as solid nanoparticles in aqueous solvents. However, this reduces sensor stability, affecting sensitivity and selectivity, and complicates mechanistic analysis. In this work, we developed homogeneous luminescence detection techniques using LnCC and investigated the underlying chemical mechanisms. The Eu-DPA coordination compound was synthesized as a homogeneous detector with good water solubility (140.3 mg/g) and excellent luminescence (92.49% quantum efficiency and >2 ms lifetime at 1 g/L concentration) via multifaceted sonication. Thanks to its homogeneous nature, the sensor achieved high sensitivity, selectivity, and stability for detecting the carcinoid marker 5-hydroxyindoleacetic acid (5-HIAA) in water, plasma, and urine. Eu-DPA exposes numerous pyridine carboxylate oxygen sites that form hydrogen bonds with the amino groups in 5-HIAA. Transient Absorption Spectroscopy (TAS) and Time-Resolved Luminescence Spectroscopy (TRES) further confirm that electrostatic interactions between the hydrogen-bonding sites significantly influence the energy transfer process, as evidenced by changes in luminescence behavior. This work not only addresses the challenge of balancing water solubility and efficient luminescence in lanthanide complexes but also offers new insights into the development of practical biomedical luminescence sensors.
研究领域:
无机化学
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分析化学
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生物无机化学
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传感器
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生物医学检测
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配位化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY
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2022
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IF 5.201
Lina Ruan,Kaiyu Guan,Yue Wang,Ming Gu,Yue Chen,Lisha Cai,Ruixuan Ye,Zhengwei Huang,Anqi Guo,Zhengkang Su,Xi Li,Jianchun Pan
Post-traumatic stress disorder (PTSD) is a serious mental disease featured by a stress dysfunction that occurs after an individual has faced intense mental stress, often accompanied by anxiety and chronic pain. Currently, the mainstream drug for PTSD is serotonin reuptake inhibitors (SSRIs), however, their pain management for patients is limited. Baicalein , a Chinese traditional herbal medicine, has shown promising results in treating anxiety, depression, and pain. In this study, we found that baicalein may alleviate single prolonged stress (SPS)-induced PTSD-like behaviors in mice without altering baseline nociceptive sensitivity or activity. Meanwhile, baicalein increased the noradrenaline (NE) and serotonin (5-HT) content and decreased the ratio of 5-hydroxyindoleacetic acid (5-HIAA)/5-HT by inhibiting the activity of monoamine oxidase A (MAO-A) in SPS-induce mice. The anxiolytic and antinociceptive effects induced by baicalein were totally abolished by 5-HT depleting agents. Moreover, the anxiolytic effects of baicalein could be abolished by the 5-HT 1A receptor antagonist WAY-100635, and the analgesic effects could be abolished by delta-opioid receptor antagonists in the spinal. Taken together, our study provides compelling evidence that baicalein reversed anxiety-like behaviors and neuropathic pain in PTSD through serotonergic system and spinal delta-opioid receptors.
研究领域:
药理学
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神经科学
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精神病学
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传统中药
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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