JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2025
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IF 4.1
Yuanyuan Dai,Yin Qin,An Lu,Li Huang,Jichao He,Qiwei Chen,Tianzuo Lan,Nannan Cheng,Lijuan Zhao,Rong Wang
Alzheimer's disease (AD) is a progressive and irreversible neurological disorder characterized by degeneration or dysfunction of central neurons. Recent studies reveal that soluble β-amyloid oligomers (AβO) serve as more reliable biomarkers for early AD detection. To address this issue, we specifically designed a dual-mode signal nanopipette sensor for AβO detection. The electrochemical signal is generated by ferrocene (Fc), while the fluorescence signal is based on Cy5. Upon capturing AβO, the double-stranded DNA aptamer (Apt1/DNA2) initiates a dual-signal process involving hybridization chain reaction (HCR). This mechanism promotes the formation of novel double-stranded DNA structures and activates the auxiliary cleavage function of the CRISPR/Cas12a system. In the presence of AβO, the fluorescence response exhibits linear correlation with AβO concentration (R 2 = 0.99557). The combined specificity of the double-stranded aptamer and CRISPR/Cas12a, coupled with the dual signal provided by HCR, significantly enhances the system's specificity and sensitivity. The detection method achieves a minimum detection limit of 2.15 pM with a linear detection range of 0.1–120 nM. Furthermore, this highly specific and sensitive system not only paves the way for detecting biomarkers of other diseases but also offers a highly promising research direction.
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Qian Bi,Hongmei Zhang,Han He,Qian Li,Baoting Dou
Doxorubicin (DOX) is a frequently prescribed chemotherapy medication for a range of cancers. It is essential to monitor the levels of doxorubicin in human biological fluids to ensure effective treatment. An efficient electrochemical sensor for amplified detection of DOX is developed in this work. The strand displacement reaction is initiated when the blocking probe, which attaches to the terminal toehold sequence of the immobilization probe, is released due to the selective recognition of DOX with the aptamer probe. The secondary toehold region is then available for hybridization with the methylene blue (MB)-labeled signal probe, causing the blocking probe to be displaced and activating the molecular machine. Consequently, the blocking probe is reused in a cyclical manner, with numerous signal probes being attached to the sensor surface, resulting in a significant increase in current for quantitative analysis. This technique successfully detected DOX in human serum samples with high selectivity and sensitivity, reaching a detection limit of 670 fM. The proposed research offers a novel approach to identifying various biomarkers, with the potential to significantly advance clinical diagnosis and drug research.
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Analytical Methods
·
2025
·
IF 2.6
Jicong Hao, Xueyan Gong, Xueyuan Duan, Weiwei Qin, Haiying Ren, Xuping ShenTu, Zihong Yea ,Xiaoping Yu
The rapid and ultrasensitive detection of parvovirus B19 (B19V) DNA is critical for preventing severe complications in high-risk populations, such as fetal hydrops in pregnant women and aplastic crisis in immunocompromised patients. The absence of clinically approved vaccines or antivirals against B19V thus mandates the urgent development of accessible in vitro diagnostics to enable time-critical interventions and contain community transmission. Herein, we developed an electrochemical biosensor as a proof-of-concept for B19V by integrating CRISPR-Cas12a with a multivalent framework nucleic acids (FNAs), namely, 12 nm tetrahedral DNA nanostructures (TDNs). Target B19V DNA activates Cas12a to indiscriminately cleave the biotin-modified ssDNA protruded from the four vertexes of the TDNs, while the TDNs precisely orient ssDNA probes on electrodes, minimizing nonspecific adsorption. This method takes advantage of the target-specific cleavage ability of CRISPR-Cas12a (Cas12a-crRNA complex, 10-12 nm) and the unique structural and functional features of 12 nm TDNs. The comparable dimensions of the Cas12a-crRNA complex and the TDN suggest a potential synergistic effect, which contributes to the observed signal amplification and high detection sensitivity. The developed platform is user-friendly, has a low detection limit (2.19 fM), and shows high selectivity. This work establishes a foundational biosensing platform, demonstrating potential for ultrasensitive nucleic acid detection. By combining the accuracy of CRISPR-Cas12a and the benefits of FNAs, this method provides a more efficient, amplification-free, and reliable approach that holds promise for future development in point-of-care diagnostics and other applications.
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CHEMICAL ENGINEERING JOURNAL
·
2026
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IF 12.5
Tingting Yu,Peng Song,Ruixue Wang,Ziwen Chen,Liang Zhao,Ming Wang,Jianying Wang,Tao Mei,Xianbao Wang,Jinhua Li
Colorectal cancer (CRC) is one of the malignant tumors with high morbidity and mortality rates worldwide. Conventional screening methods, such as endoscopy and fecal testing, are invasive and have low compliance, highlighting the urgent need for efficient, non-invasive diagnostics. Circulating small RNAs, including the sequence annotated as PIWI-interacting RNA-54265 (piR-54265), have been reported to be closely associated with CRC and represent promising serum biomarkers in liquid biopsy research. This study aims to develop an ultrasensitive, label-free solution-gated graphene transistor (SGGT) based biosensor to detect piR-54265 in serum samples, addressing limitations of current CRC biomarker detection. The Polyadenine 15 -thymine 5 (PolyA 15 T 5 -DNA) probe was designed with an L-shaped configuration, constructing a sensing interface. This configuration is proposed to facilitate a favorable probe conformation for nucleic acid recognition at the gate interface, thereby contributing to the overall sensing performance of the biosensor. The biosensor achieves a remarkable limit of detection (LOD) of 8.56 × 10 −19 M, with a linear detection range from 1 aM to 10 nM, and excellent selectivity for piR-54265. Clinical serum samples analyzed under diluted conditions demonstrate clear differentiation between CRC patients and non-cancer controls, with receiver operating characteristic (ROC) analysis further confirming its exceptional diagnostic performance and achieving an area under the curve (AUC) of 1.0. These results establish a SGGT-based sensing platform for CRC-associated RNA detection and highlight its potential for non-invasive biomarker detection and future diagnostic applications.
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ANALYTICAL LETTERS
·
2026
·
IF 1.8
Xuejun Liang,Jiuxin Cui,Kehong Liu,Jichao He,Qincong Feng,Yabo Wang
Early detection of cancer biomarkers is essential for accurate diagnosis and timely therapeutic intervention. Carcinoembryonic antigen (CEA), a nonspecific and broad-spectrum tumor-associated biomarker, is extensively employed for the early screening and diagnosis of multiple malignancies. In recent years, electrochemical nanopipette sensing technology has been increasingly explored for clinical disease detection owing to its high sensitivity, low background interference, and inherent suitability for single-cell analysis. Nucleic acid aptamers, characterized by target-specific three-dimensional conformations, are capable of binding a wide range of molecular targets with high affinity and selectivity. In this work, a functional nanopipette microelectrode was rationally designed and fabricated by immobilizing nucleic acid aptamers onto electrochemical nanopipette electrodes, enabling label-free detection of CEA at the single-cell level. Under optimized conditions, the resulting sensor achieved a limit of detection as low as 1.27 pg/mL.
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Journal of Future Foods
·
2026
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IF 7.2
Xiaobo Zhang,Zekun Long,Wei Wang,Yafeng Jiang,Qiuyue Zheng,Jijuan Cao
Aflatoxin B1 (AFB1), the most toxic mycotoxin among aflatoxin family, which presents a risk to food safety and human health at a trace level. Herein, the self-enhanced electrochemiluminescence (ECL) emitters of Au nanoclusters-anchored g-C 3 N 4 nanosheet (Au NCs@g-C 3 N 4 ) were synthesized, in which the Au NCs as a co-reaction accelerator enhanced the ECL stability and intensity. Integrating with CRISPR/Cas12a system, the Au NCs@g-C 3 N 4 behaved as sensing substrate to construct a “off-on” switch-type ECL sensor for AFB1 detection based on ECL resonance energy transfer (ECL-RET). The presence of targets AFB1 can specifically bind its aptamer to trigger the release of the activator. Using dual aptamer-regulated activation of CRISPR-Cas12a, AFB1 can be quantified via the ECL sensor, displaying a wide detection range from 0.2 pg/mL-100 ng/mL and a detection limit of 0.08 pg/mL. Furthermore, good practicability was verified in real samples assay, providing an efficient tool for the early warning of AFB1 pollution.
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ELECTROANALYSIS
·
2026
·Vol. 38
·期 1
·e70098
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IF 2.3
Qiu Jin,Xuyuan Chen,Xiaoying Wang
Excessive oligomeric Amyloid- β (A β Os) in the brain induces strong neurotoxicity and are a major causative marker of Alzheimer's disease (AD). Herein, an exemplary electrochemiluminescence (ECL) aptasensor for A β Os that coupled a triple-helix switchable system (THSS) with gold-doped Ru(bpy) 3 2+ /copper luminescent-nanospheres (RuCu@AuLNs) was successfully developed. In brief, the anti-A β Os aptamer (Apt) with two symmetric arms and RuCu@AuLN-marked signal transduction DNA (RuCu@AuLNs-ST) perfectly constructed the THSS on a gold electrode surface via hybridization reaction. At this point, the THSS was closed, and a strong initial ECL signal ( I ECL0 ) output was generated. Once A β Os appeared, they specifically bound with Apt and opened the THSS, releasing RuCu@AuLNs-ST from electrode surface, and degressive ECL signal ( I ECL1 ) was obtained. Thus, the aptasensor detected A β Os-dependent I ECL1 change (Δ I ECL1 = I ECL0 − I ECL1 ) with a concentration range of 1 fM to 1 pM with a low detection limit of 0.5 fM, and demonstrated good reliability and practicality in human serum. Additionally, the reconfigurability of the sensor allows it to be continuously reused up to 5 times at a concentration of 1 fM A β Os. The developed ECL aptasensor could be used as a cost-effective tool for early diagnosis of AD. Moreover, the strategy has promising application in the bioassays of various analytes.
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MICROCHEMICAL JOURNAL
·
2026
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IF 5.1
Sai-Nan Qin,Yi-Xue Zhang,Yi-Jie Cao,Ting Wan,Yi-Yang Zhou,Shan-Shan Su,Yu-Qiong Guo,Jian-Jun Sun,Kalle Salminen
Cisplatin (DDP), as a broad-spectrum anticancer drug, requires dynamic monitoring of its blood concentration and intervention in its dosage administration to balance efficacy and safety due to significant individual differences in its metabolism, side effects, and resistance in different patients. However, rapid and robust methods for monitoring and quantifying DDP in complex biological fluids are still lacking. This work presents an electrochemical aptamer-based ( E -AB) sensor for rapid measurement of DDP concentration in blood. In which capture probe aptamers were designed constructed by visualizing and analyzing their binding pockets, followed by molecular docking and circular dichroism to screen the aptamer Apt25, which has a small binding energy and a large conformational change, for use in the construction of the sensor. In the presence of DDP, the aptamer undergoes a conformational change at the electrode interface, altering the electron transfer rate of the electroactive label to detect DDP concentration variations. After systematically using electrochemical techniques to optimize the sensor testing conditions, the sensor can directly analyze and detect DDP in urine and blood with a measurement range of 0.001–2 mM and a detection limit of 1 μM, which satisfies the needs of clinical testing. The spiked recoveries in different biological samples were 80.8–110% and 79–115.2%, respectively. In addition, the sensor has sufficient selectivity and stability, which is expected to enable real-time monitoring of DDP concentration for dosage adjustment and improvement of drug bioavailability at the clinical site.
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ANALYTICAL CHEMISTRY
·
2026
·
IF 6.7
Aobo Feng,Zhongdi Du,Yajie Zhou,Zefeng Hua,Yudie Sun,Kui Zhang
Eutrophication-induced microcystin-LR (MC-LR), a potent hepatotoxin, poses significant threats to aquatic ecosystems and human health, necessitating the development of sensitive detection methods. In this study, we report, for the first time, a programmable gold nanoclusters (AuNCs) gel sphere used as an emitter for constructing an electrochemiluminescence (ECL) biosensor. The presence of MC-LR initiates a T7 exonuclease-mediated cyclic cleavage of the hybridization chain, releasing a single strand that captures the nanoprobe. A rolling circle amplification-synthesized, polyadenine-rich three-dimensional DNA nanohydrogel ball (DGB) serves as a dual-functional scaffold, enabling the in situ growth of AuNCs while simultaneously acting as a signal-amplifying carrier. During AuNCs synthesis, the DGB undergoes a blooming structural transition, exposing additional binding sites that hybridize with the electrode surface, thereby generating quantifiable ECL signals. The biosensor exhibits outstanding performance, with a linear detection range from 0.1 pM to 1 nM and a detection limit of 62.3 fM (S/N = 3). Its successful applications in real water and serum samples underscore its potential for environmental monitoring and clinical diagnostics, offering a novel paradigm for trace contaminant detection.
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ANALYTICAL CHEMISTRY
·
2026
·
IF 6.7
Kun Wang,Wanli Wang,Yufeng Hu,Xiaoyang Li,Haoyu Yu,Xin Xia,Tianhan Kai,Mei Wen
Staphylococcus aureus (SA) is a predominant foodborne pathogen, frequently associated with nutrient-rich matrices. However, rapid and ultrasensitive detection of SA in complex food samples remains a formidable challenge. In this work, we developed a relay biosensing system for detecting SA-16S rDNA that combines a magnetic bead-anchored APE1 DNA walker with an asymmetric CRISPR/Cas12a system. The magnetic bead-anchored DNA walker enables efficient separation of SA-targets from complex food matrices, while generating abundant activators through APE1-mediated catalytic cleavage. Employing spatially decoupled reactions with differential crRNAs, the asymmetric Cas12a system achieves programmable cascade amplification. Dual-mode fluorescence/electrochemical readout supports both laboratory analysis and on-site detection. The system demonstrates exceptional sensitivity, capable of detecting trace amounts of SA-16S rDNA (7.5 aM by fluorescence, 1.0 fM by electrochemistry) and SA (4 CFU/mL by fluorescence, 34 CFU/mL by electrochemistry). Overall, this work provides a modular and programmable biosensing strategy for bacterial identification, thereby facilitating advanced nucleic acid analysis within complex biological matrices.
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BIOSENSORS & BIOELECTRONICS
·
2026
·
IF 11.8
Siyi Zou,Jingze Li,Yancong Yu,Yourong Ou,Zhiheng Zhang,Guozhen Liu
Neuroinflammation is increasingly recognized as a critical driver in the onset and progression of Parkinson's disease (PD), yet technologies capable of continuously tracking neuroinflammatory dynamics in vivo remain limited. Here, we report an implantable bioelectronic interface that integrates multiplexed aptamer based electrochemical biosensing with deep brain stimulation (DBS) for biomarker monitoring and neuromodulation in the rat brain. The biosensing platform introduces Tantalum@Au electrodes, engineered for chronic stability and biocompatibility, enabling simultaneous in vivo electrochemical monitoring of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), α-synuclein (α-syn), and pH after DBS therapy. In freely moving PD rat models, the system maintained stable electrochemical performance for at least eight days post-implantation and captured dynamic biomarker fluctuations in response to targeted subthalamic nucleus DBS. DBS reduced IL-6 and TNF-α levels, indicating modulation of neuroinflammatory pathways, whereas α-syn level remained unchanged within the short-term window, consistent with the slower temporal dynamics of protein aggregation. DBS partially restored motor coordination though residual asymmetry persisted, paralleling clinical observations of symptom alleviation without complete functional recovery. These findings demonstrate the feasibility of integrating continuous biochemical sensing with therapeutic neuromodulation to achieve a closed-loop platform, providing direct insight into molecular responses to DBS. This bioelectronic interface establishes a foundation for future biochemical-feedback-enabled neuromodulation strategies and precision therapeutic for neurodegenerative disorders.
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Advanced Healthcare Materials
·
2026
·
IF 11
Beibei Lu,Huaibin Gong,Hua Huang,Kuaifa Fang,Kun Yu,Bitao Lu,Guangqian Lan
Acute myocardial infarction (AMI) requires rapid diagnosis beyond conventional methods, which suffer from biological interference and complexity, limiting point-of-care (POC) application. To tackle these issues, we have devised an anthraquinone (AQ)-calibrated sensor unit coupled with smartphone-based analysis for universal detection of AMI biomarkers in untreated whole blood. Our novel dual-channel biosensing platform integrates AQ as an intrinsic reference standard, establishing a self-correction mechanism to effectively counteract hematological matrix influences. The platform enables swift quantification at pg/mL levels within 5 min without sample preprocessing, achieving detection limits of 0.3, 19.7, and 0.7 pg/mL for cTnI, Mb, and CK‑MB, respectively. The smartphone‑based intelligent analyzer automates real-time signal processing, creating a closed-loop POC diagnostic setup that shows promise for future at‑home AMI risk monitoring. This technological breakthrough showcases notable resilience to biological matrix interference while upholding analytical sensitivity comparable to clinical standards, representing a significant step toward decentralized cardiovascular emergency management.
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