FOOD CHEMISTRY
·
2024
·
IF 8.5
Xian Qu,Yahui Ji,Jieyi Long,Donglin Zheng,Zhonghui Qiao,Yue Lin,Chang Lu,Yifa Zhou,Hairong Cheng
The β-1,6-glucans from Lentinus edodes have a variety of biological activities. However, the research on extraction and separation of β-1,6-glucans from L. edodes is limited and the yield is low. In the present study, we obtained the high-yield and -purity β-1,6-glucans (ALEPA) from L. edodes by using a sequential extraction and separation process, which is simple and suitable for industrialization . ALEPA significantly promoted the proliferation of splenic T lymphocytes and enhanced the phagocytosis activity of peritoneal macrophages in vivo . 16S rRNA sequencing results showed that ALEPA significantly increased the α diversity of gut microbiota and upregulated the relative abundances of short chain fatty acids (SCFAs)-producing bacterial species. Consistently, the SCFAs in the cecum of mice were upregulated. On a mechanical level, we found that the immunomodulatory effect of ALEPA depended on gut microbiota. Collectively, ALEPA is a promising functional food ingredient that regulates gut microbiota and enhances immune function.
研究领域:
食品科学
·
免疫学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SYNTHESIS-STUTTGART
·
2021
·Vol. 53
·期 14
·2435-2448
·
IF 3.157
Xin Zhou,Qing Long,Dongwei Li,Jingru Gao,Qikai Sun,Shaozi Sun,Yong Su,Peng Wang,Wenjie Peng,Ming Li
Structurally defined and pure oligosaccharides corresponding to β-glucans have attracted great attention because of their potential properties as immunostimulating agents and as antigens of vaccine candidates. We herein describe a convergent synthesis of ready-to-conjugate tridecasaccharides composed of a β-1,3-glucan nonasaccharide backbone and a β-1,6-glucan tetrasaccharide branch. The assembly was achieved by employing trichloroacetimidate glycosylations and features the gram-scale preparation of the nonasaccharide backbone and installation of the tetrasaccharide branch involving orthoester rearrangement to the glycoside.
研究领域:
有机化学
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生物化学
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疫苗研究
✓ 已验证
DOI
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Jingjing Dong,Yanli Wang,Xiaoxiao Li,Ying Chen,Rui Fan,Nana Wang,Zhengyu Jin,Yuxiang Bai
The starch-converting GH70 α-glucanotransferases, as the influential enzymatic tools for the synthesis of structurally diverse α-glucans, are of great value in the food industry. In this work, a new GtfB-type α-glucanotransferase from Fructilactobacillus sanfranciscensis Gs2 (FsGs2 GtfB) was mined from the NCBI database, which is highly conserved in four conserved regions I-IV for GtfB enzymes but possessed distinct characteristics in some residues at acceptor subsites +1/+2. Biochemical characterizations revealed that the FsGs2 GtfB enzyme demonstrated a novel 4,6-α-glucanotransferase activity, which was able to cleave the (α1 → 4) linkages in maltooligosaccharide/starch substrates and mainly synthesize branching (α1 → 4,6) linkages in the gelatinized system, resulting in the generation of low-molecular-mass short-clustered (α1 → 4,6)-glucans. Nevertheless, FsGs2 GtfB acted as a quasi-hydrolase instead of transglucosylase in the granular system, causing a notable decrease in the molecular mass of granular wheat starch and a tendency towards shortened chain lengths. Hence, the FsGs2 GtfB enzyme represents an additional potent enzymatic tool of reassembling the (α1 → 4) and (α1 → 6) linkages in starch molecules and enriches the diversity of α-glucans that can be synthesized.
研究领域:
食品科学
·
生物化学
·
酶工程
✓ 已验证
产品关联
DOI
高影响因子
Food & Function
·
2022
·Vol. 13
·期 7
·3917-3930
·
IF 6.317
Yan Qiao,Xianfeng Ye,Lingli Zhong,Chengyao Xia,Lei Zhang,Fan Yang,Yongkai Li,Xiaodong Fang,Lei Fu,Yan Huang,Hui Cao,Zhoukun Li,Zhongli Cui
The β-glucan from Saccharomyces cerevisiae is a potent adjuvant that exhibits a broad spectrum of biological activities and health benefits, and different processes have been established to prepare active β-glucan from yeast. However, studies concerning the effect of β-1,6-glucanase enzymolysis on the structure and immunomodulatory activity of yeast β-1,3-glucan are scarce. In this study, we aim to develop a novel enzymatic process for the preparation of immunologically active β-glucan (BYG) from baker's yeast using a β-1,6-glucanase GluM. The β-1,6-glucan in fungal cell wall was specifically hydrolyzed by GluM, and resulted in cell wall decomposition and β-glucan release. Batch production of BYG was realized with 17.8% yield, 85.3% purity and 75.4% recovery rate. Structural characterization indicated that BYG exhibits rod-like structures with natural triplex and nanoparticle-like substructures compared with the commercial Glucan 300. BYG ameliorated inflammation in a DSS-induced mouse model of colitis through inhibiting oxidative stress (NO, MDA and MPO), inflammatory mediators (NLRP3, ASC, caspase-1, iNOS and COX-2), and pro-inflammatory cytokines (IL-1β, IL-6, TNF-α, IFN-γ), increasing the expression levels of tight junction proteins (ZO-1, occludin and claudin-1) and modulating the production of gut microbiota-synthesized SCFAs compared to the control. Our results showed that yeast β-1,3-glucan prepared with β-1,6-glucanase exhibits structural integrity that is responsible for its favorable immunomodulatory activity.
研究领域:
微生物学
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免疫学
·
生物技术
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）