PHYTOMEDICINE
·
2026
·
IF 11.3
Ludong Tan,Xiaodong Bai,Peiyuan Yu,Tinghan Jiang,Zhe Jin
Artemisia capillaris extract alleviates acetaminophen-induced liver injury by inhibiting ferroptosis. • Capillarisin is identified as the primary bioactive constituent responsible for the hepatoprotection. • Capillarisin directly binds to and inhibits Glycogen Synthase Kinase 3β (GSK3β). • Inhibition of GSK3β by Capillarisin activates the Nrf2-mediated anti-ferroptotic pathway. • This study reveals a novel molecular mechanism for a traditional hepatoprotective herb. Background Acetaminophen (APAP) overdose is a leading cause of acute liver failure worldwide, characterized by oxidative stress-driven hepatocyte necrosis. Recent evidence implicates ferroptosis, an iron-dependent cell death, as a critical pathogenic event. Artemisia capillaris Thunb. (ACE) is a traditional hepatoprotective herb; however, its bioactive constituents and molecular targets regulating ferroptosis remain elusive. Purpose This study aimed to identify the bioactive compounds of ACE and elucidate the molecular mechanism underlying its protective effect against APAP-induced hepatotoxicity, with a specific focus on the ferroptosis pathway. Methods We integrated multi-omics, network pharmacology, and rigorous biophysical assays to decipher the protective mechanism. The ethanol extract of A. capillaris was chemically characterized using UPLC-LTQ-Orbitrap MS. An APAP-induced acute liver injury model in C57BL/6 mice and primary mouse hepatocytes were employed. Transcriptomics (RNA-seq) was utilized to identify key signaling pathways. The direct physical interaction between the active compound and its target was validated using Microscale Thermophoresis (MST), Cellular Thermal Shift Assay (CETSA), Drug Affinity Responsive Target Stability (DARTS), and molecular dynamics (MD) simulations. Functional validation was performed using gene overexpression and specific inhibitors. Results ACE pretreatment significantly attenuated APAP-induced liver injury, inflammation, and oxidative stress in mice. Transcriptomic profiling identified ferroptosis as a primary target pathway. Through chemical screening, we identified Capillarisin as the key bioactive coumarin responsible for this effect. Capillarisin treatment effectively suppressed lipid peroxidation, iron overload, and the expression of ferroptotic markers (COX2, ACSL4) while restoring GPX4 levels both in vivo and in vitro . Mechanistically, we demonstrated that Capillarisin acts as a direct inhibitor of Glycogen Synthase Kinase 3β (GSK3β). Capillarisin binds stably to the ATP-binding pocket of GSK3β ( K d = 0.87 µM), thereby inhibiting its kinase activity. This inhibition blocks the phosphorylation-dependent degradation of Nrf2, promoting its nuclear translocation and the subsequent transcriptional activation of anti-ferroptotic genes ( Gpx4, Slc7a11 ). Overexpression of GSK3β or pharmacological inhibition of Nrf2 abolished the protective effects of Capillarisin. Conclusion Our study reveals a novel mechanism whereby Capillarisin alleviates APAP-induced hepatotoxicity by directly targeting GSK3β to activate the Nrf2-mediated anti-ferroptosis defense. These findings provide a rigorous scientific basis for the ethnopharmacological use of Artemisia capillaris and highlight Capillarisin as a promising lead candidate for targeting GSK3β in the treatment of drug-induced liver injury. Download: Download high-res image (228KB) Download: Download full-size image
✓ 已验证
产品关联
DOI
PubMed
高影响因子
WATER RESEARCH
·
2026
·
IF 12.8
Huihui Peng,Mengchun Zhou,Yan Zhang,Ankang Wang,Chenying Zhou,Chuanshu He,Yongjia Lei,Peng Zhou,Bo Lai
The widespread discharge of phosphonates into aquatic ecosystems has triggered a wave of global environmental concerns, primarily due to their recognized persistence and significant ecological risks. In order to avoid the potential environmental risks associated with phosphate, the coupled strategy of oxidation and precipitation to mineralize phosphonates and immobilize the orthophosphate (o-PO 4 ) has become the main focus of current research. This strategy can be achieved through the morphological changes of iron species in the Fenton process. However, the conventional Fenton process is limited by the slow reduction rate of Fe(III), resulting in high iron-sludge output. Here, we use microscale zero-valent iron (mZVI) as an electron donor and exploit the strong iron-phosphorus coordination effect to develop a mZVI-based two-stage oxidation process (mZVI/air-Fenton). This process can achieve 75% to 100% removal of various phosphonates, and the iron sludge production is 49.6% lower than that of the Fenton process. The experimental results combined with theoretical calculations indicate that phosphonates coordinate with iron to form complexes, which induce electron redistribution on phosphonates through the ligand to metal charge transfer (LMCT) effect, reduce C-P bond energy, and form intermediates susceptible to radical attack. This coordination effect can dynamically remove the passivation layer on mZVI and promote the continuous activation and release of mZVI. In addition, this coordination effect reduces the redox potential of Fe(III)/Fe(II) by 0.14 V through intramolecular charge transfer, which greatly speeds up the cycle of iron, improves the utilization efficiency of iron electrons, and increases the yield of hydroxyl radical ( • OH) by 3.1 times. Therefore, this study provides an innovative improved Fenton oxidation process for the efficient treatment of organophosphorus wastewaters and significant iron-sludge reduction.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2026
·
IF 8.2
Yifan Li,Xiangyi Du,Zhiyi Wang,Zijian Lin,Yusen Liu,Sinan Chen,Xiumei Wang,Linfeng Nong,Peng Zhang,Mingzhang Lin
Defects play a pivotal role in enhancing hydrogen production from water radiolysis. To elucidate the influence of defects in boron nitride on radiolytic hydrogen evolution, boron nitride samples with various nitrogen defects (denoted as Nv-BN1, Nv-BN2, and Nv-BN3) were prepared through ball milling, gamma irradiation, and thermal calcination, respectively. Through these operations, samples with different relative densities of three-boron center nitrogen defects (TBC defects) were successfully obtained. Among the synthesized materials, the Nv-BN1 sample with an ultralow solid loading (0.075 wt %) achieved a saturated radiolytic hydrogen yield of 0.65 × 10–7 mol J–1, while Nv-BN2 (0.025 wt %) with higher defect density exhibited an even greater saturated yield of 0.70 × 10–7 mol J–1. In contrast, the calcined Nv-BN3 showed reduced activity due to the passivation of defect sites. Fluorescence experiments revealed elevated levels of both •OH and eaq– in suspensions containing TBC defect samples compared to pure water, suggesting that water molecules at the defect-rich solid–liquid interface undergo enhanced radiolytic dissociation. Notably, the •OH yield was lower than that of eaq–, implying that partial consumption of •OH occurred at electron-deficient boron sites. This hypothesis is supported by X-ray time-resolved in situ infrared spectroscopy, which showed the gradual emergence of a characteristic B–O band near 1190 cm–1 during irradiation, a spectral feature absent in pristine h-BN sample. Complementary density functional theory (DFT) simulations further corroborate this mechanism, suggesting that water molecules preferentially adsorb at TBC defect sites, where the intramolecular O–H bond is weakened, thereby facilitating bond cleavage. Collectively, these results highlight the unique role of TBC defects as catalytic centers that enhance water radiolysis, offering fundamental mechanistic insights into defect-mediated interfacial chemistry and presenting a promising strategy for advancing radiation-driven energy conversion technologies.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2026
·
IF 8.2
Licong Xu,Cunxu Gong,Minghua Wu,Deyou Yu
Waste sericin was valorized into Fe 3 O 4 / N -doped porous biochar Fenton-like catalyst. • Molecular coordination enabled uniform dispersion of active Fe 3 O 4 nanoparticles. • 0.1Fe-NC degraded 99.3% AO-7 with kobs of 0.266 min −1 via PMS activation. • Multiple ROS including Fe(IV)=O and 1 O 2 dominated the reaction. • Packed-bed continuous-flow operation kept >98% AO-7 removal for 720 min. Developing sustainable catalysts from waste biomass for advanced oxidation processes is highly desirable for wastewater treatment. Herein, a waste-to-resource strategy was proposed to upcycle silk-industry waste sericin into Fe 3 O 4 -anchored N -doped porous biochar Fenton-like catalysts (Fe-NC) for peroxymonosulfate (PMS) activation. Distinct from conventional biomass-derived Fe/carbon catalysts that often require external nitrogen dopants or post-loading of metal species, sericin served simultaneously as a carbon source, intrinsic nitrogen precursor, and Fe 3+ -binding biopolymer, enabling the coordination-assisted formation of Fe 3 O 4 / N -doped carbon interfacial active sites after controlled pyrolysis. The optimized 0.1Fe-NC possessed a high specific surface area of 477.9 m 2 g −1 and uniformly dispersed Fe 3 O 4 nanoparticles, enabling rapid removal of Acid Orange 7 (AO-7) over a wide pH range of 4–11. Nearly complete degradation of AO-7 (99.3%) was achieved within 30 min, with a pseudo-first-order rate constant ( k obs ) of 0.266 min −1 . Mechanistic investigations revealed that multiple reactive oxygen species, including Fe(IV)=O, SO 4 -, OH, and 1 O 2 , jointly contributed to pollutant degradation. In addition, the catalyst exhibited excellent magnetic recoverability and stability, maintaining 89.7% removal efficiency after five cycles. Continuous-flow experiments further demonstrated stable performance (>98% removal for 720 min), highlighting its practical applicability. This work provides a sustainable strategy for converting protein-rich sericin waste into efficient Fe-based biochar catalysts and offers insights into designing biomass-derived PMS activation systems with integrated heteroatom doping, metal anchoring, and interfacial electron-transfer regulation. Download: Download high-res image (166KB) Download: Download full-size image
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Expert Opinion on Drug Metabolism & Toxicology
·
2025
·
IF 3.4
Yongbin Tong,Qi Zhang,Yutong Chen,Zhiqiang Zhao,Jingqi Li,Jinyi Wang,Haishan Xia,Danyi Lu,Yuyu Liu
Introduction Coumarin exhibit various biological activities, including anti-inflammatory, antioxidant and anxiolytic effects. However, its clinical application is restricted due to significant hepatotoxicity observed in multiple animal models. CYP2A5 is the primary enzyme responsible for metabolizing coumarin in the mouse liver, homologous to human CYP2A6.Methods Acute toxicity and pharmacokinetic investigations were conducted on Rev-erbα−/− and wild-type mice following coumarin administration. The regulatory effects of REV-ERBα on CYP2A5 expression were assessed both in vivo and in vitro. Western blotting and qPCR were used to measure the protein and mRNA levels, respectively.Results Firstly, coumarin-induced hepatotoxicity was mitigated in Rev-erbα−/− mice. Secondly, the systemic exposure of coumarin was lowered in Rev-erbα−/− mice compared with wild-type mice. Thirdly, deletion of Rev-erbα resulted in significant upregulation of CYP2A5 mRNA and protein expression in mice. REV-ERBα negatively regulated CYP2A5/2A6 expression in Hepa-1c1c7 and HepG2 cells. Furthermore, luciferase reporter and chromatin immunoprecipitation (ChIP) assays indicated that REV-ERBα modulated Cyp2a5 transcription and expression through the repression of DBP.Conclusions The knockout of Rev-erbα significantly reduces the hepatotoxicity of coumarin by modulating CYP2A5. This research underscores the potential of targeting the REV-ERBα/CYP2A5 axis as a novel strategy for reducing coumarin-induced hepatotoxicity.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2025
·
IF 11.3
Yiming Sun,Chenying Zhou,Shuang Meng,Shuang Zhong,Yang Liu,Chuanshu He,Peng Zhou,Bo Lai
Heteroatom doping has been applied as an excellent method for mediating the catalytic reactivity of carbon materials. To overcome the inherent defect of slow Fe(III) reduction in Fenton chemistry, this work finds that boron-doped carbon materials demonstrate high cocatalytic activity in boosting Fenton oxidation. During Fenton chain reactions catalyzed by boron-doped reduced graphene oxide (B-rGO, the typical boron-doped carbon material), highly reactive near-free Fe(III) formed on the B-rGO surface can dramatically promote H2O2 mediated Fe(III) reduction, which strongly accelerates the rate-determining reaction in Fenton chain reactions to generate hydroxyl radicals. The results of DFT calculations, electrochemical analysis, characterizations (XAFS, in situ Raman, and HAADF-STEM), and pH-dependent performance reveal that doped boron atoms can modulate the electron-deficient iron atom of FeOH2+ by stretching the Fe–O bond (from 163.6 to 179.2 pm), which forms near-free Fe(III) on the B-rGO surface with higher oxidation capability than free Fe(III), and the Fe(III) oxidation potential is positively and linearly related to the boron content of B-rGO (R2 = 0.96). Moreover, near-free Fe(III) can dramatically oxidize H2O2 (a green electron donor) to accelerate Fe(II) regeneration, thereby promoting Fenton chain reactions for long-lasting Fenton oxidation. These discoveries afford a strategy for designing green and sustainable cocatalysts to overcome the intrinsic weaknesses of classical Fenton chemistry.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
·
IF 8.6
Qinqin Yu,Youzhi Dai,Zhu Zhang,Bo Feng
A novel photo-Fenton coupling system was constructed in this paper to address the problems of low visible light utilization, narrow pH applicability range and difficult separation and recovery of common photo-Fenton. Fe single-atom material with high activity was used as a bridge connecting photocatalysis and Fenton oxidation, and the performance and mechanism of the coupling system were investigated using a typical antibiotic ciprofloxacin (CIP) as the target pollutant. It was found that the degradation performance of the photo-Fenton coupling system is significantly improved, and can be maintained in acidic, neutral and alkaline wastewaters; the main active species of the coupling system are ·O 2 – , 1 O 2 and h + , and the enhancement mechanism is a combination of Fe single atoms, S-scheme heterojunction and multiple active species.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2022
·
IF 14.224
Limin Duan,Huihao Jiang,Wenhao Wu,Daohui Lin,Kun Yang
Introducing crystal defects into iron based metal-organic frameworks (Fe-MOFs) is regarded as a promising strategy to enhance Fenton-like performance. However, developing a facile and effective strategy to construct defective Fe-MOFs as highly efficient Fenton-like catalyst is still a challenge. Herein, MIL-100(Fe) (Def-MIL-100(Fe)) with missing ligands defects was synthesized by a simple heterogeneous reaction using zero-valent iron. The bisphenol A degradation efficiency in the Def-MIL-100(Fe)/H 2 O 2 system reached up to 91.26% within 10 min at pH 4 with a low catalyst dosage of 0.05 g/L, while the perfect MIL-100(Fe) has almost no Fenton-like performance. It was observed that missing ligands defects in the Def-MIL-100(Fe) play a key role in the Fenton-like reaction. The missing ligands defects could increase the Lewis acidity for fast H 2 O 2 adsorption and accelerate the electron transfer between Fe II and Fe III cycling, leading to faster and more·OH generation. Moreover, the missing ligands defects could promote the mass transfer for improving·OH utilization efficiency. This work provides a novel strategy to construct defective Fe-MOFs as highly efficient Fenton-like catalyst to degrade organic pollutants in water.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2023
·
IF 11.4
Shuang Meng,Minglu Sun,Peng Zhang,Chenying Zhou,Chuanshu He,Heng Zhang,Yang Liu,Zhaokun Xiong,Peng Zhou,Bo Lai
The continuous electron supply for oxidant decomposition-induced reactive oxygen species (ROS) generation is the main contributor for the long-standing micropollutant oxidation in the iron-based advanced oxidation processes (AOPs). Herein, as a new class of co-catalysts, metal borides with dual active sites and preeminent conductive performance can effectively overcome the inherent drawback of Fenton-like reactions by steadily donating electrons to inactive Fe(III). Among the metal borides, tungsten boride (WB) exhibits a significant co-catalytic performance run ahead of common heterogeneous co-catalysts and exceptionally high stability. Based on qualitative and semi-quantitative tests, the hydroxyl radical, sulfate radical, and iron(IV)-oxo complex are all produced in the WB/Fe(III)/PDS system and Fe(IV)-induced methyl phenyl sulfoxide decomposition is up to 72%. Moreover, the production efficiency of ROS and relative proportions of radical and nonradical pathways change with various experimental conditions (dosages of PDS, WB, and solution pH) and water matrices. The rate-determining step of Fe(II) regeneration is greatly accelerated resulting from the synergetic effect between exposed metallic reactive sites and nonmetallic boron with reductive properties of WB. In addition, the self-dissolution of surface tungsten oxide and boron oxide leads to a renovated surface for sustainable Fe(III) reduction in long-term operations. Our discovery provides an efficient and sustainable strategy in the field of enhanced AOPs for water remediation.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2023
·
IF 9.5
Shunni Dong,Qianqian Zheng,Meiqi Tang,Shaoxiong Zhu,Jingjing Nie,Binyang Du
Chromic materials play a decisive and escalating role in information security. However, it is challenging to develop chromic materials for encryption technologies that can hardly be imitated. Inspired by versatile metachrosis in nature, a series of coumarin-based 7-(6-bromohexyloxy)-coumarin microgel colloidal crystals (BrHC MGCC) with multiresponsive chromism are able to be assembled by ionic microgels in poly(vinyl alcohol) (PVA) solution followed by two cycles of freezing–thawing. The ionic microgels can be finely tailored by in situ quaternization with tunable size under varied temperatures and hydration energies of counterions as well as quenched luminescence under UV irradiation, which endows BrHC MGCC with intriguing chromism in the dual-channel coloration of physical structural color and chemical fluorescent color. Three types of BrHC MGCC exhibit various change ranges in structural coloration and similar quenching in fluorescence emission, which can be utilized for the development of the static–dynamic combined anticounterfeiting system with dual coloration. The information conveyed by the BrHC MGCC array presents dynamic variation versus temperature, while the static information can be only integrally read in both sunlight and a 365 nm UV lamp. The fabrication of a microgel colloidal crystal with dual coloration opens a facile and ecofriendly window for multilevel information security, camouflage, and a cumbersome authentication process.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS ES&T Engineering
·
2023
·
IF 7.1
Kui Yang,Daoyuan Zu,Zhong Zhang,Jinxing Ma,Zhifeng Yang
A reactive electrochemical membrane (REM) has been identified as an excellent platform in micropolluted water purification due to its rapid mass transfer capability. Unlike traditional flow-by systems, the convection-enhanced REM systems activate the REM’s inner reaction sites, potentially imparting a spatially variable reactivity. Therefore, we explored the spatial scale of micropollutant oxidation in REM. Theoretical and experimental evidence demonstrated that an REM might feature nonuniform oxidation activity along its reaction depth (about hundreds of micrometers). That is, the zone generating •OH was located within a few micrometers beneath the REM surface, i.e., less than 1% of its reaction depth due to the rapid overpotential/potential drop in the REM. The nonuniform reactive character might render the REM of a strong direct electron transfer ability, enabling rapid removal of electron-rich micropollutants even at low potentials. However, unsatisfactory removal of electron-poor micropollutants has been observed, and a higher potential was required to prompt the generation of •OH. These results provide in-depth insight into the spatial scale of nonuniform oxidation of micropollutants in REM, contributing to the analysis of micropollutant oxidation mechanisms and the optimization of the design and operation of REM modules for cost-effective water purification.
✓ 已验证
产品关联
DOI
高影响因子
APPLIED CATALYSIS B-ENVIRONMENTAL
·
2023
·
IF 22.1
Xinyu Song,Yang Shi,Zelin Wu,Bingkun Huang,Xinhao Wang,Heng Zhang,Peng Zhou,Wen Liu,Zhicheng Pan,Zhaokun Xiong,Bo Lai
The discriminative activities and mechanisms for activation of O-O bond in peroxy compounds via single-atom catalysts (SACs) especially with a higher coordination number (M-N 5 ) are rarely explored. Herein, the atomic catalyst (Fe-SAC) with Fe-N 5 as the active center was constructed, which could simultaneously and effectively activate peroxymonosulfate (PMS), peroxydisulfate (PDS), and hydrogen peroxide (H 2 O 2 ). Density functional theory (DFT) calculations combined with experiments results demonstrated that the degradation efficiencies of acyclovir were corresponding to the changes of O-O bond length in various peroxy compounds. Meanwhile, the experiments exposed that the three oxidants all produced • OH, but the PMS also contained 1 O 2 and Fe(IV)=O, and PDS contained O 2 •- additively. The scale-up experiment showed Fe-SAC could operate steadily for nearly 7 d. Overall, this work unveils the discriminative mechanisms for activation of O-O band in different Fenton-like systems. Moreover, the Fe-SAC with high coordination numbers has excellent catalytic properties and practical performance.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）