BRITISH JOURNAL OF PHARMACOLOGY
·
2026
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IF 7.5
Jing Jin,Yao Jin,Rong Zhang,Ying Li,Zhi-Ying Zhou,Jin-Peng Feng,Yan-Tong Liu,Zhi-Jie Sun,Zhi-Yin Xiao,De-Li Dong
Background and Purpose Bladder cancer remains a significant burden on public health globally. Carbon monoxide (CO) has emerged as a promising therapeutic agent for cancers because of its key role in inducing mitochondrial dysfunction and metabolic reprogramming in cancer cells; however, its clinical translation has been hindered by systemic toxicity because of its binding to haemoglobin, which is avoided by localised intravesical therapy via the bladder. This study aimed to develop and characterise a novel manganese(I)-based CO releasing molecular MnCORM as a therapeutic agent against bladder cancer. Experimental Approach A new MnCORM chelate of a quinoline derivative was synthesised and characterised, and its anti-cancer efficacy was assessed using a 2D bladder cancer cell model, a 3D tumour spheroid model, and a murine orthotopic bladder cancer model. Molecular mechanisms were explored via staining assays, transmission electron microscopy (TEM) and western blots. Key Results MnCORM inhibited cell viability and induced cell death in T24 and RT112 bladder cancer cells. MnCORM activated AMPK, whereas the AMPK inhibitor Compound C mitigated MnCORM-induced cytotoxicity in T24 cancer cells. MnCORM induced AMPK-triggered and Drp1-dependent mitochondrial fission, promoted reactive oxygen species (ROS) accumulation, and induced mitophagy-associated cell death. The inhibitory effect of MnCORM on tumour growth was validated in a 3D tumour spheroid model and further proven in an orthotopic bladder cancer model in nude mice after intravesical instillation of MnCORM (1 mg kg −1 ). Conclusions and Implications Our work shows that MnCORM could be potentially applied for bladder cancer therapy through intravesical instillation with less systemic toxicity. Graphical
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
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IF 8.6
Fan Wu,Hao Li,Yang Pan,Yingjia Wang,Yonghui Sun,Jianming Pan
The rapid and accurate recovery of precious metal palladium from waste resources has always been a hot and challenging topic. In addition, from the perspective of energy and environmental protection, the regeneration and rational use of waste are also crucial for a sustainable future. Here, an 8-aminoquinoline-modified core–shell magnetic nano stirring robot (MNSR@PGMA-AQ) towards Pd(Ⅱ) is reported which not only has ultra-high solid–liquid separation efficiency but also can accelerate the mass transfer rate in adsorption through self-stirring. A series of adsorption experiments show that MNSR@PGMA-AQ has fast adsorption kinetics, reaching 10.97 mg g −1 in 20 min, which is 85.87 % of the equilibrium adsorption capacity. And selectivity parameters such as distribution coefficient ( K D ), separation factor ( α ) and concentration factor ( CF ) demonstrate its excellent adsorption selectivity for Pd(Ⅱ). Moreover, it is proved that the adsorption mechanism of MNSR@PGMA-AQ towards Pd(Ⅱ) is ion exchange and chelation, which mainly depends on the functional monomer 8-aminoquinoline. The regeneration and palladium recovery experiments in actual samples further prove the application value of this novel robot. Furthermore, Pd-loaded MNSR@PGMA-AQ was used as a catalyst for the Suzuki-Miyaura cross-coupling reaction and investigated its catalytic performance to pursue the goal of waste regeneration and responsible catalyst manufacture.
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JOURNAL OF MATERIALS SCIENCE
·
2022
·Vol. 58
·期 1
·170-185
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IF 4.682
Zang Yu,Gao Shan,Jing Boyu,Sun Hong,Wang Jianjun,Liu Jiao,Miao Fengjuan,Xu Liang
Four kinds of conjugated microporous polymers (CMPs) with imine structures were synthesized by a Sonogashira coupling reaction followed by a postreaction between aldehydes and amines. Palladium nanoparticles (Pd NPs) were successfully supported on the CMPs to prepare Pd/CMP heterogeneous catalysts and were used to catalyze the Suzuki–Miyaura coupling reaction. The number of aromatic nuclei and nitrogen atoms in the CMPs highly affects Pd loading. In the Suzuki–Miyaura coupling reaction, the Pd/CMP with good Pd NPs dispersibility and appropriate Pd content (Pd/CMP-NA) exhibited the highest catalytic efficiency with 100% conversion and 95.4% yield which can be used for many substrates. The Pd/CMP-NA heterogeneous catalyst was easy to recover and still exhibited good catalytic efficiency after 5 cycles. To further understand the catalytic mechanism of Pd/CMP-NA on the Suzuki–Miyaura coupling reaction, we proposed a rational catalytic cycle. This paper provides an idea for the structural design of CMPs used for Suzuki–Miyaura catalytic reaction.
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JOURNAL OF HAZARDOUS MATERIALS
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2022
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IF 14.224
Fan Wu,Hao Li,Yang Pan,Yonghui Sun,Jianming Pan
Quick and precise recovery of palladium (Pd) from electronic waste remains a serious task, owing to the strong acid and complexity of chemical compounds in leachate. Here, bioinspired construction of magnetic nano stirring rod with radially aligned dual mesopores and abundant 8-aminoquinoline (MNSR-DM-AQ) is proposed for selective and rapid extraction of Pd(II) from highly acidic sample solutions. Benefit from the unique dual mesoporous (12.4 nm and 3.6 nm) and the stirring motion under an external magnetic field, MNSR-DM-AQ possesses enhanced adsorption capacity and kinetics, achieving 11.62 mg g −1 (97.2 % of the maximum adsorption capacity) in 15 min. Distribution coefficient ( K D = 299.0 mL g −1 ), separation factor ( α above 25.54) and concentration factor ( CF = 230.2 mL g −1 ) reveal the excellent selectivity of MNSR-DM-AQ towards Pd(II) when comparing with the coexisting ions (Ca(II), Co(II), Cu(II), Fe(II), Mg(II), Ni(II), Pb(II), Zn(II)). The adsorption mechanisms of MNSR-DM-AQ are ion exchange and chelation due to a strong affinity between Pd(II) and N. Meanwhile, 96.82 % of the captured Pd(II) can be easily eluted within 15 min, and the adsorption capacity remains stable after five adsorption-desorption cycles. It is worthwhile to mention that MNSR-DM-AQ exhibits a high adsorption capacity of 8.39 mg g −1 from leachate of abandoned high-voltage patch capacitor, which is greatly desired in Pd(II) extraction from electronic waste.
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DYES AND PIGMENTS
·
2022
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IF 5.122
Selvaraj Muthusamy,Siqi Yin,Kanagaraj Rajalakshmi,Suci Meng,Dongwei Zhu,Meng Xie,Jimin Xie,Rathna Silviya Lodi,Yuanguo Xu
Hydrazine is a highly toxic pollutant to the environment and living organisms. Therefore, it is an urgent need to detect hydrazine selectively, sensitively, and successfully in the real samples and biosystems . Thus, we designed a donor-π-acceptor fluorophore quinoline ( Q ) appended 4-formylphenyl 2,4-dinitrobenzoate ( P-DNP ) to form a Schiff base QP-DNP for tracking hydrazine in real samples and biosystems. The probe observes an intramolecular charge transfer and aggregation induced emission sensing mechanisms on cleavage of ester bond upon nucleophilic substitution reaction with hydrazine and exhibited a “turn-on” fluorescence at green channel with the formation of quinoline-phenol. The proposed study was investigated through photophysical properties, 1 H NMR titration, HR-MS experiments, and density-functional theory calculations. The LOD of QP-DNP for hydrazine was calculated to be 55 nM and exhibited a high selectivity among possible competitive analytes. Additionally, the probe showed a remarkable emission colour changes on fabrication of paper sensor strips that can be discriminated in monitoring hydrazine vapor using smartphone and achieved an AND gate operation. Furthermore, QP-DNP was successfully applied to fluorescence imaging of hydrazine in live HeLa cells , monitoring of in situ hydrazine release as a metabolite of isoniazid drug administrated orally and determining hydrazine contamination in real samples.
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Analytical Methods
·
2023
·Vol. 15
·期 40
·5329-5340
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IF 3.1
Yixuan Yao,Fudong Zeng,Liangqiang Wu,Shuo Xing,Qingbiao Yang,Yaoxian Li
We have successfully synthesized a near-infrared fluorescent probe for the continuous detection of copper and sulfur ions. The probe has good selectivity and anti-interference ability against Cu2+ and S2−. The results show that after adding Cu2+ to the DL solution of the near-infrared fluorescent probe, Cu2+ forms a [DL + Cu2+] complex with the probe, which leads to fluorescence quenching due to the paramagnetism of Cu2+. The probe can be used for the quantitative detection of Cu2+ with a detection limit of 1.26 × 10−9 M. According to the Job's plot curve the binding stoichiometry between DL and Cu2+ is 1 : 1. Subsequently, S2− was added to the [DL + Cu2+] solution, because the precipitation dissolution equilibrium constant of CuS was Ksp = 1.27 × 10−36, so the binding capacity between Cu2+ and S2− was stronger, CuS precipitation was formed, and red fluorescence was re-released, and the quantitative detection of S2− was realized, and the detection limit was 3.50 × 10−8 M. Through bacterial imaging experiments, we found that the probe can accomplish the fluorescence imaging experiments of Staphylococcus aureus, indicating that the probe has good biopenetration and biocompatibility, and has application prospects in bioimaging and environmental monitoring. In addition, the probe DL has good suitability for Cu2+ and S2− detection in real samples.
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RSC Advances
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2021
·Vol. 11
·期 17
·10264-10271
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IF 3.361
Xinyu Wang,Zhuo Li,Jiaojiao Nie,Liangqiang Wu,Weihong Chen,Shaolong Qi,Hai Xu,Jianshi Du,Yaming Shan,Qingbiao Yang
Copper is an essential element in living systems and plays an important role in human physiology; therefore, methods to detect the concentration of copper ions in living organisms are important. Herein, we report a highly water-soluble naphthalimide-based fluorescent probe that can be used for the detection of Cu2+. The probe, BNQ, has high selectivity and sensitivity. The fluorescence intensity of the probe at 520 nm was visible to the naked eye under a UV lamp; upon the gradual addition of Cu2+, there was a colour change from green to nearly colourless. Furthermore, the detection limit of BNQ for Cu2+ was 45.5 nM. The detection mechanism was investigated using a Job's plot and density functional theory (DFT) calculations. In addition, owing to great biocompatibility, we were able to successfully use BNQ to detect Cu2+ in living HeLa cells with low toxicity.
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DYES AND PIGMENTS
·
2021
·
IF 4.889
Selvaraj Muthusamy,Long Zhao,Kanagaraj Rajalakshmi,Dongwei Zhu,Rodah Soy,John Mack,Tebello Nyokong,Shengjun Wang,Kang-Bong Lee,Weihua Zhu
Tracking the biothiol cysteine (Cys) in living systems is a significant responsibility to balance the redox environment and oxidative stress. A quinoline-7-nitro-1,2,3-benzoxadiazole ( Q-NBD ) fluorophore has been synthesized and characterized towards examination of Cys. The probe forms a quinoline-substituted phenol ( Q-Ph-OH ) after thiolysis of the NBD ether bond, leading to an increase of fluorescence at green channel. The turn-on sensing mechanism originates from the change in intramolecular charge transfer (ICT-OFF) along with an aggregation-induced emission (AIE) as suggested by spectroscopy measurements in solutions, time-dependent density-functional theory (TD-DFT) calculations and 1 H NMR titration examination. Importantly, Q-NBD exhibited great sensitivity with a low limit of detection value of 89.5 nM and remarkable selectivity in various biothiols towards Cys. The sensor probe was successfully used for detecting both endogenous and exogenous Cys in PC3 living cells and spiked Cys in human urine samples.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2020
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IF 3.232
Selvaraj Muthusamy,Kanagaraj Rajalakshmi,Qingxiang Xu,Yan Chen,Long Zhao,Weihua Zhu
Detection of H 2 S to analyze some diseases in living lives demands fast response, high selectivity and biocompatibility. Here we designed an azide containing coumarin attached with 8-aminoquinoline via amide backbone (ACAQ) fluorophore as the H 2 S sensing probe. Excellent response time of 6 min, high sensitivity with the limit of detection (LOD) of 14.6 nM and high selectivity with other possible interferences are revealed for ACAQ after characterized by spectroscopy, 1 H NMR titration and LC–MS measurements. The sensing strategy is explained by amide-iminol tautomerism and azide reduction. In addition, the successful visualization measurement suggests the practicability of the probe ACAQ for H 2 S detection in live samples.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2021
·
IF 4.098
Brian Musikavanhu,Selvaraj Muthusamy,Dongwei Zhu,Zhaoli Xue,Qian Yu,Choonzo N. Chiyumba,John Mack,Tebello Nyokong,Shengjun Wang,Long Zhao
A new Schiff base probe ( QT ) consisting of 8-aminoquinoline (Q) and thiophene-2-carboxaldehyde (T) moieties has been synthesized. QT undergoes chelation-enhanced fluorescence quenching when exposed to Hg 2+ due to coordination by the sulfur and nitrogen atoms of QT thus forming a facile “turn-off” sensor. The formation of the chelation complex was confirmed by UV–visible absorption and emission spectral measurements, 1 H NMR titration and density functional theory calculations. These studies revealed that the probe exhibits high selectivity and sensitivity towards Hg 2+ in the presence of other common metal ions. A low detection limit of 23.4 nM was determined and a Job plot confirmed a 2:1 stoichiometry between QT and Hg 2+ . The potential utility of QT as a sensor for Hg 2+ ions in human HeLa cells was determined by confocal fluorescence microscopy, and its suitability for use in the field with environmental samples was tested with Whatman filter paper strips.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2021
·
IF 4.098
Kanagaraj Rajalakshmi,Selvaraj Muthusamy,Yun-Sik Nam,Yujun Li,Kang-Bong Lee,Yuanguo Xu
Developing new reaction based recognizing units can enhance the specificity of target analyte molecules in practical applications of real samples and biosystems. Therefore, introducing a recognizing moiety diphenylborinate was encountered for the detection of pyruvate biomolecule through Lewis acid-base reaction based sensing strategy. The construction of the Schiff-base back bone between quinoline and N-(diethylamino)salicylaldehyde-diphenylborinate ( QSB) were expressed the red shift from blue emission of quinoline in to green as that of dative bond developed between Schiff base nitrogen and boron atoms. The new sensing approach was involved such a way that fluorophore QSB is a Lewis acid while pyruvate acts as Lewis base, where the elimination of Lewis pair produced a weak green fluorescence including the formation of quinoline, N-(diethylamino)salicylaldehyde ( QS ). The switching products were witnessed through 1 H NMR titration, HR-MS and FT-IR studies. The good selectivity and interference ability were achieved in presence of 1000-fold excess of possible interferences with LOD of 16 nM. Moreover, the tracking ability of the probe was employed towards pyruvate in live HeLa cell imaging for evaluating an exogenous and endogenous signals producing ability and its mitochondria targeting property was investigated successfully. Further, the practical utility of the probe was tested with milk samples and obtained good recovery results.
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TALANTA
·
2021
·
IF 6.057
Selvaraj Muthusamy,Long Zhao,Kanagaraj Rajalakshmi,Dongwei Zhu,Shengjun Wang,John Mack,Kang-Bong Lee,Long Zhang,Weihua Zhu
This work describes (Z)-N-((Z)-2-(1,3,3-trimethylindolin-2ylidene)ethylidene)quinoline-8-amine (LYSO-QF), a high-performing and biocompatible dye comprised of quinoline and Fisher aldehyde moieties linked via an imine vinyl backbone with lysosome targeting ability that can be used to quantitatively detect the mercury ion (Hg 2+ ) in biosystems and the natural environment. This is achieved by forming three different tetrameric, trimeric and dimeric complexes between Hg 2+ and LYSO-QF with the limit of detection (LOD) of 11 nm. The complexes formed were analyzed with the aid of time-dependent density functional theory (TD-DFT) calculations. The concentration dependence of the Hg 2+ complex fluorescence emission changes from grey-green to jade green and then to red as the different types of complex are formed. The favorable sensor properties of the LYSO-QF probe are demonstrated by monitoring different Hg 2+ concentrations in buffer solutions, HeLa cells, zebrafish model samples and several different types of water sample. Experiments with Whatman paper strips demonstrate that the cost-effective LYSO-QF also has considerable potential for use in on-site Hg 2+ detection with the naked eye.
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