ANALYST
·
2021
·Vol. 146
·期 22
·6893-6901
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IF 4.616
Hui Chen,Longqiang Xing,Huiru Guo,Caixia Luo,Xuedian Zhang
A graphene oxide (GO)-based nanocarrier that imparts tumor-selective delivery of dual-drug with enhanced therapeutic index, is introduced. GO is conjugated with Au@Ag and Fe3O4 nanoparticles, which facilitates it with SERS tracking and magnetic targeting abilities, followed by the covalent binding of the anti-HER2 antibody, thus allowing it to both actively and passively target SKBR3 cells, human breast cancer cells expressed with HER2. Intracellular drug delivery behaviors are probed using SERS spectroscopy in a spatiotemporal manner, which demonstrates that nanocarriers are internalized into the lysosomes and release the drug in response to the acidic microenvironment. The nanocarriers loaded with dual-drug possess increased cancer cytotoxicity in comparison to those loaded with a single drug. Attractively, the enhanced cytotoxicity against cancer cells is achieved with relatively low concentrations of the drug, which is demonstrated to be involved in the drug adsorption status. These results may give us the new prospects to design GO-based delivery systems with rational drug dosages, thus achieving optimal therapeutic response of the multi-drug with increased tumor selectivity and reduced side effects.
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Journal of Ginseng Research
·
2021
·
IF 6.06
Chenglong Sun,Shuangshuang Ma,Lili Li,Daijie Wang,Wei Liu,Feng Liu,Lanping Guo,Xiao Wang
Background Panax notoginseng is a highly valued medicinal herb used widely in China and many Asian countries. Its root and rhizome have long been used for the treatment of cardiovascular and hematological diseases. Imaging the spatial distributions and dynamics of metabolites in heterogeneous plant tissues is significant for characterizing the metabolic networks of Panax notoginseng , and this will also provide a highly informative approach to understand the complex molecular changes in the processing of Panax notoginseng . Methods Here, a high-sensitive MALDI-MS imaging method was developed and adopted to visualize the spatial distributions and spatiotemporal changes of metabolites in different botanical parts of Panax notoginseng . Results A wide spectrum of metabolites including notoginsenosides, ginsenosides, amino acids, dencichine, gluconic acid, and low-molecular-weight organic acids were imaged in Panax notoginseng rhizome and root tissues for the first time. Moreover, the spatiotemporal alterations of metabolites during the steaming of Panax notoginseng root were also characterized in this study. And, a series of metabolites such as dencichine, arginine and glutamine that changed with the steaming of Panax notoginseng were successfully screened out and imaged. Conclusion These spatially-resolved metabolite data not only enhance our understanding of the Panax notoginseng metabolic networks, but also provide direct evidence that a serious of metabolic alterations occurred during the steaming of Panax notoginseng .
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JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2021
·
IF 3.205
Xiao-Pan Liu,Wen-Qian Sun,Tong-Xin Liu,Bing-Bing Liu,Chang-Po Chen
Due to the strong background interferences in the low-mass region and poor reproducibility of conventional organic matrices, it is of great importance to develop a novel matrix for matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) to qualitatively and quantitatively analyze small molecules. In this work, water-soluble fullerenol C 60 (OH) 24–26 was selected as a MALDI matrix for the analysis of low-molecular-weight compounds in consideration of optical absorption property, water solubility and stability. Compared with the traditional matrices, fullerenol demonstrated lower background interference and stronger peak intensity. In addition, the hydrophilic fullerenol could avoid the heterogeneous crystallization in sample preparation, increase the reproducibility and sensitivity of MALDI-MS, and ameliorate quantitative analysis of small molecules. With saccharin as model analyte, quantitative analysis was carried out using fullerenol as matrix. The results demonstrated satisfying reproducibility and good tolerance to salt. The limit-of-detection of the quantitative analysis was as low as 4 pmol, and the linear range is 1–100 μg mL −1 with R 2 greater than 0.99. The analytical results also showed excellent precision and accuracy, low matrix effect and good recovery rate. Fullerenol as a potential matrix was further validated in the quantification of saccharin sodium in different real food samples, such as nuts and drinks. This work not only confirms the potential of fullerenol for the quantitative analysis in food field, but also provides a new technique for rapid analysis of small molecules.
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TOXICOLOGY LETTERS
·
2023
·
IF 3.5
Jingjing Qu,Yinjing Zhang,Chunxia Liu,Qianqian Xie,Tong Ou,Bolin Fan,Yan Song
Anthraquinone is a recently identified contaminant present in teas globally, and its potential teratogenic and genotoxic impacts have yet to be fully comprehended. Hence, this study's objective was to determine anthraquinone's genotoxicity using various studies such as the Ames test , Mammalian erythrocyte micronucleus test, and in-vitro mammalian chromosome aberration study. Additionally, the study assessed its effects on maternal gestational toxicity and the fetus's teratogenicity through prenatal developmental toxicity research in rats. Results indicated that anthraquinone did not manifest mutagenic effects on Salmonella typhimurium histidine-deficient, did not cause chromosomal aberrations in Chinese hamster ovary cell subclone CHO-K1, and did not exhibit a genotoxic effect on mouse bone marrow erythrocytes. However, in the prenatal developmental toxicity study, administering anthraquinone orally to pregnant rats from day 5 to day 19 of gestation resulted in decreased body weight and food consumption of pregnant rats, along with a higher number of visceral malformations in the fetuses in the highest dose group (217.6 mg/kg BW). Additionally, two pregnant rats died in this group. The study has established the no observed adverse effect level (NOAEL) as 21.76 mg/kg BW, while the lowest observed adverse effect level (LOAEL) was 217.6 mg/kg BW.
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JOURNAL OF MASS SPECTROMETRY
·
2024
·Vol. 59
·期 7
·e5058
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IF 1.9
Xintong Zhao,Juan Ren,Ze Wang,Xiangfeng Chen
Analysis of noncovalent interactions between natural products and proteins is important for rapid screening of active ingredients and understanding their pharmacological activities. In this work, the intensity fading MALDI-TOF mass spectrometry (IF-MALDI-MS) method with improved reproducibility was implemented to investigate the binding interactions between saponins from Panax notoginseng and lysozyme. The benchmark IF-MALDI-MS experiment was established using N , N ′, N ″-triacetylchitotriose-lysozyme as a model system. The reproducibility of ion intensities in IF-MALDI-MS was improved by scanning the whole sample deposition with a focused laser beam. The relative standard deviation (RSD) of deposition scanning IF-MALDI-MS is 5.7%. Similar decay trends of the relative intensities of notoginseng saponins against increasing amounts of lysozyme were observed for all six notoginseng saponins. The half-maximal fading concentration (FC 50 ) was calculated to quantitatively characterize the binding affinity of each ligand based on the decay curve. According to the FC 50 values obtained, the binding affinities of the six notoginseng saponins were evaluated in the following order: notoginsenoside S > notoginsenoside Fc > ginsenoside Rb1 > ginsenoside Rd > notoginsenoside Ft1 > ginsenoside Rg1. The binding order was in accordance with molecular docking studies, which showed hydrogen bonding might play a key role in stabilizing the binding interaction. Our results demonstrated that deposition scanning IF-MALDI-MS can provide valuable information on the noncovalent interactions between ligands and proteins.
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FITOTERAPIA
·
2024
·
IF 2.5
Wenjun Cai,Shuo Zhang,Yaonan Wang,Changli Liu,Rong Luo
Aurantii Fructus Immaturus (AFI) was structurally divided into two parts named “peel” and “pulp”. The exocarp and mesocarp of materials named “peel”. The endocarp separated into multiple compartments and the cystic hair attached to it named “pulp”. In order to explore the distribution and content of constituents in AFI, an efficient method to explore the distribution of constituents was developed based on matrix assisted laser desorption/ionization fourier transform ion cyclotron resonance mass spectrometry imaging (MALDI-FTICR-MSI). After simple processing, thirty-two constituents with distinct localization in the mass range of 101–1200 Da were identified by MALDI-FTICR-MSI. In addition, the identified four flavnoids (poncirin, sinensetin, 3,5,6,7,8,3′,4′-heptemthoxyflavone, and tangeritin) were analyzed for differences between using LC-MS. Quantitative analysis results supported the quantitative results from MALDI-FT-ICR-MSI. The results implied that different parts had different constituents in AFI, and demonstrated MALDI-MSI have high potential in the direct analysis of constituents.
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JOURNAL OF CHROMATOGRAPHY A
·
2025
·
IF 4
Xu Zhang,Xiangbo Ji,Zifang Peng,Yanhao Zhang,Zongwei Cai,Shusheng Zhang
Due to the complicated sample matrix and low concentration, analysis of small-molecule emerging environmental pollutants generally required complex sample preparation and long instrumental detection. It guarantees the sensitivity but is not conducive to rapid screening. In this work, a p -aminoazobenzene ( p -AAB) with excellent energy absorption capability was used to modify multilayer Ti 3 C 2 T X (MXene) to prepare novel material p -AAB/MXene. The modification significantly improved the laser absorption of original material, and made p -AAB/MXene could be employed as matrix for surface-assisted laser desorption/ionization-time of flight-mass spectrometry (SALDI-TOF MS) analysis of small-molecule emerging pollutants. More importantly, p -AAB/MXene could be used as adsorbent to enrich target compounds, then directly sent to SALDI-TOF MS detection without any other sample pre-treatment. Dual characteristics of enrichment material and matrix made p -AAB/MXene-based SALDI-TOF MS method successfully be applied to rapid and accurate detection of emerging environmental pollutants, p -phenylenediamine-quinones (PPDQs) and diamide insecticides (DAIs), in food (beverage) and environmental (PM 2.5 ) samples. High sensitivity (LOD at ng mL -1 level) and satisfactory precision (RSD < 11%) indicated the qualified analytical performance of developed approach. The determined concentrations also elucidated the broad occurrence of PPDQs and DAIs in beverages and PM 2.5 . The results further confirmed the SALDI-TOF MS using p -AAB/MXene as both adsorbent and matrix had considerable potential for efficient and time-saving analysis of trace and ultra-trace small-molecule emerging environmental organic pollutants in complicated samples.
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Frontiers in Pharmacology
·
2024
·
IF 4.4
Wu Lirong,Wang Jieyi,Lei Jing,Ge Kun,Qu Chun,Liu Jiajian,Huang Fengjie,Sun Dongnan,Chao Xiaowen,Chen Tianlu,Zhao Aihua,Jia Wei,Zheng Xiaojiao,Xie Guoxiang
Background: Porcine bile powder (PBP) is a traditional Chinese medicine that has been used for centuries in various therapeutic applications. However, PBP has not previously undergone comprehensive component analysis and not been evaluated for safety through standard in vivo toxicological studies.Methods: In our study, we characterized the component of PBP by liquid chromatography-mass spectrometry. The acute and subchronic oral toxicity, genotoxicity, and teratogenicity studies of PBP were designed and conducted in Kunming mice and Sprague-Dawley (SD) rats.Results: The chemical analysis of PBP showed that the main components of PBP were bile acids (BAs), especially glycochenodeoxycholic acid. There were no signs of toxicity observed in the acute oral test and the subchronic test. In the genotoxicity tests, no positive results were observed in the bacterial reverse mutation test. Additionally, in the mammalian micronucleus test and mouse spermatocyte chromosomal aberration test, no abnormal chromosomes were observed. In the teratogenicity test, no abnormal fetal development was observed.Conclusion: Our findings demonstrate that PBP, composed mainly of BAs, is non-toxic and safe based on the conditions tested in this study.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2024
·Vol. 416
·期 29
·6869-6877
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IF 3.8
Cui Xiaoqing,Geng Haoyuan,Guo Huanying,Wang Lei,Zhu Zihan,Zhang Yaqi,Chen Panpan,Wang Xiao,Sun Chenglong
Berberine (BR), an alkaloid isolated from the Chinese traditional medicine Coptidis rhizoma, exhibits therapeutic effects on several diseases including bacterial infections, diabetes, and hyperlipidemia, but the oral availability is poor. In this work, we prepared the chitosan microneedle array–loaded BR (BR-CS MNAs) to transdermally deliver BR, and the spatial distribution of BR in heterogeneous skin tissues was analyzed and imaged by matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI). Some endogenous phospholipids with specific spatial distribution were used to differentiate the epidermis and dermis regions of the skin. The results showed that BR was effectively delivered and could permeate to both epidermis and dermis regions of the skin. This demonstrated the feasibility of MALDI-MSI to evaluate the transdermal delivery efficiency of microneedle arrays and suggested BR could be transdermally delivered by CS MNAs. Graphical Abstract The chitosan microneedle array–loaded BR (BR-CS MNAs) was prepared and used to transdermal deliver BR. The spatial distribution of BR during the transdermal deliver process was analyzed and imaged by matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI). Some endogenous phospholipids with specific spatial distribution were used to help differentiate the epidermis and dermis regions of skin
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ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Xiaoqing Cui,Shiping Chen,Haoyuan Geng,Panpan Chen,Yaqi Zhang,Min Li,Xiao Wang,Chenglong Sun
Cancer cells are marked by metabolic reprogramming. Mapping metabolites and their related functional proteins contributes to a comprehensive understanding of metabolic regulation during cancer progression. Mass spectrometry imaging enables the visualization of metabolites in heterogeneous cancer tissues. However, direct mass spectrometry analysis of functional proteins is challenging due to their high molecular weight and low ionization efficiency. Herein, we developed three mass probes for matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) analysis of Ki67, glutaminase, and arginase on biological samples. Mass probes were created by assembling thiolated mass tags (MTs) and specific antibodies on gold nanoparticles (AuNPs). The probes were able to target Ki67, glutaminase, and arginase for MALDI-MSI analysis. Thiolated MTs were dissociated online during MALDI-MSI. This empowered the conversion of Ki67, glutaminase, and arginase signals to MT ions, realizing functional protein analysis by MALDI-MSI. Additionally, MALDI-MSI was able to detect related metabolites directly in adjacent tissue sections, providing complementary data on metabolites and their related functional proteins. This helped to deepen our understanding of cancer metabolism from multiple perspectives.
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