APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
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2014
·Vol. 174
·期 8
·2818-2826
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IF 1.687
Shu H.,Xu L.,Li Z.,Li J.,Jin Z.,Chang S.
Banana is an important tropical fruit worldwide. Parthenocarpy and female sterility made it impossible to improve banana varieties through common hybridization. Genetic transformation for banana improvement is imperative. But the low rate that banana embryogenic callus was induced made the transformation cannot be performed in many laboratories. Finding ways to promote banana somatic embryogenesis is critical for banana genetic transformation. After tobacco arabinogalactan protein gene NtEPc was transformed into Escherichia coli (DE3), the recombinant protein was purified and filter-sterilized. A series of the sterilized protein was added into tissue culture medium. It was found that the number of banana immature male flowers developing embryogenic calli increased significantly in the presence of NtEPc protein compared with the effect of the control medium. Among the treatments, explants cultured on medium containing 10 mg/l of NtEPc protein had the highest chance to develop embryogenic calli. The percentage of lines that developed embryogenic calli on this medium was about 12.5 %. These demonstrated that NtEPc protein can be used to promote banana embryogenesis. This is the first paper that reported that foreign arabinogalactan protein (AGP) could be used to improve banana somatic embryogenesis.
研究领域:
植物学
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遗传学
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分子生物学
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DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
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2024
·
IF 3.3
Kaihua Liu,Zhijie Liu,Junjian Miao,Yiqun Huang,Keqiang Lai
BACKGROUND N ε-carboxymethyllysine (CML), N ε-carboxyethyllysine (CEL) and α -aminoadipic acid (AAA) are important foodborne hazards and their intake can cause a variety of diseases in humans. It is extremely important to investigate the formation mechanism of CML, CEL and AAA, as well as their association with each other when aiming to control their production. RESULTS A multi-response kinetic model was developed within the glucose-lysine Maillard reaction model system. The concentrations of glucose, lysine, glyoxal (GO), methylglyoxal (MGO), CML, CEL and AAA were quantified at different temperature (100–160 °C) and at different intervals (0–60 min). The experimental data were fitted to the proposed model to calculate kinetic parameters for the corresponding steps. The results indicated that the production of CML was primarily relied on the direct oxidative cleavage of the Amadori product, rather than the reaction between GO and Lys, whereas CEL and AAA were generated through the reaction of MGO with Lys. Significantly, the reaction between α -dicarbonyl compounds and Lys preferentially generated CML and CEL, resulting in the lower concentrations of AAA compared to CML and CEL. CONCLUSION The multi-response kinetic model developed in the present study can be applied well to the Maillard reaction. The relationship between the formation mechanisms of CML, CEL and AAA is also explained. © 2024 Society of Chemical Industry.
研究领域:
食品科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
Sustainability
·
2022
·Vol. 14
·期 21
·14300
·
IF 3.889
Qinghong Wang,Siyu Li,Xin Wang,Zhuoyu Li,Yali Zhan,Chunmao Chen
The extensive use of pharmaceuticals and personal care products (PPCPs) causes high concentrations of pharmaceutical metabolites to exist in aquatic environments. Though the removal of parent PPCPs has raised concerns, the degradation of pharmaceutical metabolites was rarely investigated. In this study, the degradation of 4-acetylaminoantipyrine (4-AAA), a typical dipyrone metabolite frequently detected worldwide in surface water and wastewater, was initially studied using persulfate (PDS)-based advanced oxidation processes (AOPs). Compared with commonly used activation methods of alkali, ultrasonic, ultraviolet, and Fe2+, 4-AAA achieved its best degradation (98.9%) within 30 min in a thermally activated PDS system due to the promotion of both radical production and the reaction rate with the rise in temperature. The optimum degradation of 4-AAA could be achieved with the temperature of 80 °C regardless of initial pH values, indicating a wide suitable pH range. Moreover, over 80% of the degradation of 4-AAA could be achieved with the presence of Cl−(0–16 mM), HCO3−(0–8 mM), and humic acid (0–30 mg/L), further indicating the application potential of the system. Both sulfate radicals (SO4•−) and hydroxyl radicals (•OH) contributed to 4-AAA degradation and the contribution of •OH increased with the pH rising from 3 to 11 due to the transformation from SO4•−when reacting with OH−. Three hydroxylated and ring-opening intermediates were detected during the 4-AAA degradation. The ECOSAR prediction indicated that the acute toxicity of most intermediates decreased than 4-AAA while the chronic toxicity increased, which suggested the transformation of intermediates should be further focused on in SO4•−and •OH based AOPs. This study would provide technical reference for the control of 4-AAA in wastewater treatment processes, raise concerns on the influence of PPCPs metabolites, and throw light on reducing the harm of PPCPs and their metabolites in aquatic environments.Keywords:4-acetylaminoantipyrine (4-AAA);heat activation;persulfate;degradation pathway;toxicity
研究领域:
环境科学
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水处理
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高级氧化过程
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DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2025
·
IF 6.1
Yicheng Wang,Miaoyun Qiu,Bixuan Yue,Mingliang Wang,Luoxing Xia,Fengfeng Li,Chaobo Yang,Zhigao Song,Yushan Liao,Wei Liu,Zhi Zeng
Di-(2-ethylhexyl) phthalate (DEHP), a widely utilized plasticizer, has been increasingly associated with cardiovascular risks. However, the involvement of DEHP in abdominal aortic aneurysm (AAA) remains unclear. To address this gap, C57BL/6 J mice were intragastrically administered with DEHP for 4 weeks in a CaCl₂-induced AAA model. We found that DEHP exacerbated AAA progression, as evidenced by a notable enlargement in the maximal diameter of the abdominal aorta, enhanced vascular inflammation, and accelerated elastin degradation. In vitro experiments confirmed that DEHP, along with its primary metabolite mono-(2-ethylhexyl) phthalate (MEHP), induced M1 macrophage polarization, leading to increased secretion of inducible nitric oxide synthase (iNOS), matrix metalloproteinase-9 (MMP9), and pro-inflammatory factors, including tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β). Mechanistically, we demonstrated that DEHP and MEHP triggered the activation of the nuclear factor-kappa B (NF-κB) pathway by upregulating phosphorylated p65 and inducing p65 nuclear translocation, which drove M1 macrophage polarization. These findings were further confirmed using a mouse AAA model in vivo. Additionally, DEHP and MEHP promoted the proliferation and migration of primary human aortic vascular smooth muscle cells (VSMCs). Phenotypic switching of VSMCs was markedly accelerated following DEHP and MEHP treatment, potentially through the PI3K-AKT pathway, as characterized by reducing contractile markers (ACTA2 and CNN1) and enhancing proliferative markers (PCNA and SPP1). Overall, our study underscores the pivotal role that DEHP plays in exacerbating AAA by promoting M1 macrophage polarization and VSMCs phenotypic switching, which provides new insights into the adverse health effects of environmental pollution-relevant cardiovascular disease progression.
研究领域:
环境毒理学
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心血管疾病
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分子生物学
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免疫学
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细胞生物学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）