Marine Drugs
·
2024
·Vol. 22
·期 11
·513
·
IF 4.9
Xiaohan Zang,Yongcheng Wang,Cong Han,Lishuang Cui,Haojie Liu,Shuimiao Tian,Kechun Liu,Peihai Li,Chen Sun,Qing Xia,Yun Zhang
Hyperlipidemia and consequent endothelial inflammation, along with foam cell generation, promote the progression of atherosclerosis (AS). Here, we aimed to investigate the effects of 2-acetamidophenol (2-AAP), which was selected by zebrafish phenotypic screening, in alleviating AS by relieving hyperlipidemia and inhibiting foam cell formation, as well as the underlying mechanisms. In a zebrafish hyperlipidemia model, 2-AAP increased lipid-lowering efficacy; alleviated TC, TG, LDL-C, and MDA levels; elevated HDL-C and T-SOD levels; significantly improved intravascular macrophage aggregation; and improved blood flow. In an ox-LDL-induced RAW264.7 model, 2-AAP inhibited lipid phagocytosis in RAW264.7 cells; reduced the intracellular TC, TG, FC, and CE contents; and decreased the CE/TC ratio, thus slowing foam cell generation. In addition, 2-AAP alleviated intracellular ROS and ferrous ion accumulation in RAW264.7 cells, reduced the MDA content, and increased GPX4 viability. Furthermore, transcriptome analyses and gene expression validation showed 2-AAP treatment upregulates genes related to GSH synthesis and transport, such as gclc, gclm, gss, and gpx4a, and enhanced the expression levels of genes involved in the storage and transportation of iron ions, such as fpn1, fth, and g6pd, indicating that 2-AAP dramatically regulated the ferroptosis and glutathione metabolic pathways. Overall, our study demonstrated that 2-AAP demonstrated potential in AS by alleviating hyperlipidemia and attenuating the ferroptosis pathway and provided evidence supporting the future application of 2-AAP in AS treatment.
研究领域:
心血管疾病
·
药理学
·
分子生物学
·
细胞生物学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PROGRESS IN ORGANIC COATINGS
·
2022
·
IF 6.206
Qing Xiang,Yujie Qiang,Lei Guo
In this study, 4-amino-antipyrine (AAP) was grafted on graphene oxide (GO) via the hydrothermal strategy (GO@AAP) to improve the corrosion resistant property of epoxy coating . Transmission electron microscope (TEM), scanning probe microscope (SPM) and Raman techniques were employed to investigate the morphology and structure of GO@AAP nanohybrid , which also effectively confirmed the successful AAP grafted on GO surface . Electrochemical and salt spray results indicated that incorporating as-synthesized GO@AAP into epoxy coating powerfully enhanced the anti-corrosion property, which was greatly consistent with the mild steel morphology after corrosion experiment. Besides, after 60 days of immersion, the |Z| 0.01 Hz was 3.7 × 10 8 Ω·cm 2 , which was two order of magnitude higher than that of pure epoxy coating. Local electrochemical impedance spectroscopy (LEIS) was visually demonstrated the synergistic effect of physical shielding and corrosion inhibition of GO@AAP in 3.5 wt% NaCl solution. This work provides a new method to achieve the barrier and inhibition activities of epoxy coatings, which are suitable for heavy corrosion resistant application in harsh environment.
研究领域:
材料科学
·
腐蚀与防护
✓ 已验证
DOI
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 11
·1-9
·
IF 5.3
Zhang Shengyuan,Yang Ying,Yang Dezhi,Yang Yaling
A metal–organic-framework (MOF) fluorescent sensor is reported based on NH 2 -MIL-101(Fe) propelled pesticide and alkaline phosphatase (ALP) catalytic reaction. Different from previous reports, a cascade reaction system combined with MOF structural changes to generate fluorescence was employed. The rationale is that ALP can hydrolyze L-ascorbic acid 2-phosphate (AAP) into L-ascorbic acid (AA), which can reduce Fe 3+ to decompose structurally NH 2 -MIL-101(Fe), resulting in 2-aminoterephthalic acid (NH 2 -BDC) with intense fluorescence. The fluorescence can be decreased to different degrees due to inhibition of organophosphate pesticides (OPPs) on the activity of ALP. By taking chlorpyrifos (CPY) as the model compound of an OPP pesticide and adding ALP and CPY into the NH 2 -MIL-101(Fe) framework, the resulting cascade reaction fluorescence sensors exhibit a good sensitivity for CPY and ALP sensing. The working ranges are 0.02–2 μg/L and 0.2–20 mU/mL with detection limits (LOD) of 5.31 ng/L and 0.05 mU/mL, respectively. The proposed sensor has been actually applied to satisfactory detection of CPY and ALP in food and serum samples. This fluorescence-based assay may extend the application of MOF-based biosensors. Graphical Abstract
研究领域:
分析化学
·
环境科学
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生物化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Ning Xia,Fengli Gao,Gang Liu,Yong Chang,Lin Liu
Background Small molecule mimics offer the advantages of easy preparation, good thermodynamic stability, and reproducible catalytic activity. However, most of the reported organic artificial mimics face challenges including low catalytic activity, oxidative self-destruction, and auto-aggregation into inactive dimers. Therefore, novel organic mimics with high catalytic activity as well as good thermal and environmental stability are highly desirable. Results We found that pyrroloquinoline quinone (PQQ), a nutritionally important growth factor, displayed significant peroxidase-like activity to catalyze the oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) into blue oxidized TMB (oxTMB) by H 2 O 2 . Its catalytic activity surpassed that of other organic small molecules reported previously, including fluorescein derivatives and adenosine/guanosine triphosphate. Based on the reduction of oxTMB by the enzymatically generated ascorbic acid (AA), the PQQ/TMB-based system was used to monitor alkaline phosphatase (ALP) activity with ascorbic acid 2−phosphate (AAP) as the substrate. This sensing system allowed for the determination of ALP with a detection limit of 1 mU/mL. Furthermore, we discovered that PQQ can coordinate with Cu 2+ to form metal-organic nanoparticles. The building blocks of two active cofactors endowed the nanoparticles with impressive functionalities for biocatalytic applications. With Cu-PQQ nanoparticles as signal labels, colorimetric immunoassays of prostate specific antigen (PSA) were achieved with a lowest detectable concentration down to 0.1 pg/mL. Significance This work is valuable for the design of novel biosensors by utilizing PQQ as an artificial enzyme because of its high catalytic activity, good stability, ease of storage, and simple chemical structure.
研究领域:
生物化学
·
分析化学
·
纳米技术
·
生物传感器
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Yujing Kong,Fang Yuan,Fei Yang,Cuiling Zhang,Yuezhong Xian
Manipulating cell–cell interactions is of great significance in cell communication and cell-based therapies. Although efforts have been made to construct cell–cell assembly by stimuli-responsive host–guest interactions, controllable cell–cell interactions by near-infrared (NIR) light triggered reversible assembly remain a challenge. Herein, we develop a NIR-controlled system based on β-cyclodextrin (β-CD) modified upconversion nanoparticles (UCNPs) for reversible and noninvasive manipulation of cell assembly and disassembly, which is realized by host–guest interactions between E/Z-photoisomerization of arylazopyrazole (AAP) and β-CD under the NIR irradiation. UCNPs can convert NIR to ultraviolet light, which leads to the transformation of AAP from the E-isomer to the Z-isomer. And it can be reverted back to the E-isomer under visible light irradiation. This reversible photoisomerization can modulate the host–guest interaction between β-CD and AAP, thus leading to reversible cell assembly and disassembly. Furthermore, by precise regulating cell–cell interactions by NIR light, cell–cell communication and molecular transportation can be realized. Given the diversity of host and guest molecules and the advantages of NIR light in biological applications, reversible cell–cell assembly has great potential for the regulation of cell behaviors and cell-based therapies.
研究领域:
生物医学工程
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纳米技术
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细胞生物学
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治疗学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 7
·1-8
·
IF 5.3
Han Lei,Cai Shuanglong,Chen Xiaogeng
The development of an ultrasensitive and precise measurement of a breast cancer biomarker (cancer antigen 15-3; CA15-3) in complex human serum is essential for the early diagnosis of cancer in groups of healthy populations and the treatment of patients. However, currently available testing technologies suffer from insufficient sensitivity toward CA15-3, which severely limits early large-scale screening of breast cancer patients. We report a versatile electrochemical immunoassay method based on atomically cobalt-dispersed nitrogen-doped carbon (Co-NC)–modified disposable screen-printed carbon electrode (SPCE) with alkaline phosphatase (ALP) and its metabolite, ascorbic acid 2-phosphate (AAP), as the electrochemical labeling and redox signaling unit for sensitive detection of low-abundance CA15-3. During electrochemical detection by differential pulse voltammetry (DPV), it was found that the Co-NC-SPCE electrode did not have a current signal response to the AAP substrate; however, it had an extremely favorable response current to ascorbic acid (AA). Based on the above principle, the target CA15-3-triggered immunoassay enriched ALP-catalyzed AAP produces a large amount of AA, resulting in a significant change in the system current signal, thereby realizing the highly sensitive detection of CA15-3. Under the optimal AAP substrate concentration and ALP catalysis time, the Co-NC-SPCE-based electrochemical immunoassay demonstrated a good DPV current for CA15-3 in the assay interval of 1.0 mU/mL to 10,000 mU/mL, with a calculated limit of detection of 0.38 mU/mL. Since Co-NC-SPCE has an excellent DPV current response to AA and employs split-type scheme, the constructed electrochemical immunoassay has the merits of high preciseness and anti-interference, and its clinical diagnostic results are comparable to those of commercial kits. Graphical abstract
研究领域:
分析化学
·
生物化学
·
电化学
·
癌症诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Xiaodan Zhang,Ai Yuan,Xuanxiang Mao,Qiumeng Chen,Yuming Huang
We report that the Co/Mn oxides nanocomposite, obtained by directly pyrolying cobalt doped Mn-BTC precursor, is a new oxidase mimetic for colorimetric sensing of acid phosphatase (ACP). By tuning the molar ratios of cobalt ions to Mn-BTC (BTC = 1,3,5-benzenetricarboxylate) precursor, four bimetal Co/Mn-BTCs and their derivatives were obtained. These Co/Mn oxides showed 1.4-fold to 2.6-fold higher oxidase-like activity than that of Mn 2 O 3 derived from Mn-BTC, indicating that Co-doping is an effective way for improving and tuning oxidase-like activity of manganese oxides. The rambutan-like Co/Mn oxides with Co/Mn molar ratio of 0.33 (denoted CMO-0.33) in the MOFs precursors exhibited the highest oxidase-like activity. The estimated Michaelis constant for 3,3′,5,5′-tetramethylbenzidine (TMB) was 2.26-fold lower than that of Mn 2 O 3 , indicating its stronger affinity to TMB. Based on the inhibitory effect of ascorbic acid (AA) on the TMB-CMO-0.33 system, a new colorimetric strategy was paved for screening ACP activity when coupled with L-ascorbic acid 2-phosphate trisodium salt (AAP) because ACP could catalyze hydrolysis of AAP to generate AA. The proposed colorimetric biosensor allows detection of ACP in the range 0.02–1.0 U/L with a limit of detection of 8.2 mU/L. Its potential utilization in ACP assay in human serum samples is successfully demonstrated.
研究领域:
材料科学
·
纳米技术
·
分析化学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
Nanoscale Advances
·
2023
·
IF 4.7
Guo-Ying Chen,Mao-Ling Luo,Li Chen,Tong-Qing Chai,Jia-Li Wang,Ling-Xiao Chen,Feng-Qing Yang
The combined application of nanozymes and natural enzymes has received widespread attention in recent years. In this work, a simple and efficient method was used to synthesize a composite material of CuO nanoparticles modified zeolitic imidazolate framework-8 (CuO NPs@ZIF-8) with multiple enzyme activities (glucose oxidase-like and hydrolase-like activities) to detect the activity of natural enzymes through fluorescence and colorimetric (UV-Vis) dual-mode detections. The hydrolase- and oxidase-like activities of CuO NPs@ZIF-8 show an acceptable affinity with L-ascorbic acid 2-phosphate trisodium (AAP) and o-phenylenediamine (OPD). Using the developed sensor, highly sensitive detection of natural enzymes glucose oxidase (GOX) and alkaline phosphatase (ALP) was achieved through both fluorescent and colorimetric analysis with a wide linear range (fluorescence for GOX: 0.86-1.23×105 mU/mL, UV-Vis for GOX: 0.081-1.62×105 mU/mL; fluorescence for ALP: 0.042-1.20×104 mU/mL, UV-Vis for ALP: 0.0046-1.23×104 mU/mL) and low LOQs (fluorescence for GOX: 0.86 mU/mL, UV-Vis for GOX: 0.081 mU/mL; fluorescence for ALP: 0.042 mU/mL, UV-Vis for ALP: 0.0046 mU/mL). Compared to the other fluorescent and colorimetric sensors, this sensor has better catalytic activity due to the addition of GOX and ALP, which can amplify the detection signal and improve the sensitivity. This is the first time that composite material CuO NPs@ZIF-8 with "tandem enzyme" activity was synthesized and applied in the detection of enzyme activity. Additionally, the proposed fluorescent and UV-Vis platforms exhibit the capability to detect GOX and ALP in serum samples with satisfactory recovery, indicating potential application prospects in biochemical analysis.
研究领域:
分析化学
·
生物化学
·
纳米技术
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Shi Gang Liu,Lei Han,Na Li,Yu Zhu Fan,Yu Zhu Yang,Nian Bing Li,Hong Qun Luo
The development of ratiometric fluorescent strategy is very significant and challenging in bioanalysis. Herein, graphitic carbon nitride (g-C 3 N 4 ) nanosheets and cobalt oxyhydroxide (CoOOH) nanoflakes are exploited for ratiometric fluorescence assay of alkaline phosphatase (ALP) activity. In the g-C 3 N 4 /CoOOH nanohybrid, g-C 3 N 4 nanosheets serve as a signal unit and the CoOOH nanoflakes function as a recognition element, and initially, the fluorescence of g-C 3 N 4 is quenched by the CoOOH nanoflakes. In the absence of target, the CoOOH nanoflakes of the nanohybrid system are able to oxidize o-phenylenediamine (OPD), and the resultant oxidation product (OxOPD) quenches the blue emission of g-C 3 N 4 and meanwhile emits orange fluorescence which acts as another signal element. However, an efficient redox reaction between ascorbic acid (AA) and CoOOH can cause decomposition of the CoOOH nanoflakes, and additionally, ALP can catalytically hydrolyze L-ascorbic acid-2-phosphate (AAP) to generate AA. Thus, in the presence of target, the CoOOH nanoflakes are destroyed by AA preferentially and the OPD is rarely oxidized to OxOPD, accompanied with strong blue emission of g-C 3 N 4 and weak orange fluorescence from OxOPD. Target-dependent dual-signal change made the ratiometric assay possible, and also AA-induced signal variation was investigated and attributed to the stronger reducing capacity of AA than OPD. The ratiometric sensing platform for ALP activity assay provides a new perspective for the applications of two-dimensional nanomaterials to develop novel and sensitive biosensors.
研究领域:
生物化学
·
分析化学
·
纳米技术
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Ting Ting Li,Hui Xi Li,Qiang Yu,Wen Qing Jia,Shu Ting Li,Wei Zhu,Zhen Chen,Lei Han
It is vital to exploit sensitive and portable means for the reliable assay of acid phosphatase (ACP) activity and its inhibitors screening in clinical diagnosis and drug therapy. Herein, the Pt-Ni alloy nanoparticles (Pt-Ni ANPs) with enhanced oxidase-mimetic activity were successfully prepared and applied to oxidize the achromatous 3,3′,5,5′-tetramethylbenzidine (TMB) to blue oxTMB. The oxTMB, as an effective photothermal agent, can transform photon energy to heat under an 808 nm laser illumination. The absorbance and temperature of the reaction solution increased significantly when TMB was oxidized to oxTMB by the oxidase-like Pt-Ni ANPs. However, the ACP can hydrolyze 2-Phospho-L-ascorbic acid trisodium salt (AAP) to produce ascorbic acid (AA), and then the production of AA, as a powerful reductant, can efficiently reduce oxTMB and react with singlet oxygen ( 1 O 2 ) to suppress the oxidase-mimicking activity of Pt-Ni ANPs, causing an obvious decrease in absorption spectrum and temperature of the assay system. A colorimetric and photothermal dual-mode detection platform was established for ACP activity assay by integrating colorimetric and temperature signals. Moreover, the potential application of this strategy was also verified by sensing ACP activity in human serum samples and its inhibitor screening. This dual-mode sensing platform not only improves sensitivity and precision but also meets the needs of diversity detection, which offers new insights for efficient monitoring of ACP activity and inhibitor screening in resource-poor settings.
研究领域:
分析化学
·
生物传感器
·
医学诊断
·
纳米技术
·
临床化学
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2013
·
IF 5.437
Mo Wang,Huanshun Yin,Nannan Shen,Zhenning Xu,Bing Sun,Shiyun Ai
In this work, a photoelectrochemical (PEC) biosensor was fabricated for sensitive and specific detection of microRNA based on Bi 2 S 3 nanorods and enzymatic signal amplification . Using the catalytic effect of alkaline phosphatase on l -ascorbic acid 2-phosphate trisodium salt (AAP), ascorbic acid (AA) was in situ generated and used as electron donor . Based on this, a signal-on protocol was successively achieved for microRNAs detection due to the dependence of photocurrent response on the concentration of electron donor of AA. The results demonstrated that the photocurrent response enhanced with increasing the hybridized concentration of microRNA. Under the amplification of the immunogold labeled streptavidin (SA-AuNPs), a low detection limit of 1.67 fM was obtained. The fabricated biosensor showed good detection stability and specificity, and it could discriminate only one-base mismatched microRNA sequence. Moreover, the down-regulated expression of microRNA-21 in DF-1 chicken fibroblast cells infected with subgroup J avian leukemia virus (ALVs) was confirmed by the developed method, indicating that microRNA-21 might be a new biomarker for avian leukemia. This work opens a different perspective for microRNAs detection and early diagnose of avian leukemia.
研究领域:
生物传感器
·
分子生物学
·
病毒学
·
癌症研究
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 12
·1-9
·
IF 5.833
Tang Zhuoyun,Wei Zeliang,Huang Ke,Wei Yinhao,Li Dongdong,Yan Shixin,Huang Jin,Geng Jia,Tao Chuanmin,Chen Piaopiao,Ying Binwu
Human immunodeficiency virus (HIV) infection inflicts significant economic and social burdens on many countries worldwide. Given the substantial morbidity and mortality from HIV infection, there is an urgent need for accurate and early detection of the virus. In this study, immunofluorescence and visual techniques are described that detect the HIV-1 p24 antigen, which relied on selective recognition of Ag + /Ag nanoparticles (Ag NPs) and Cu 2+ /Cu + using cadmium telluride quantum dots (CdTe QDs). After the sandwich immunoreactions were accomplished, the alkaline phosphatase (ALP) hydrolyzed L-ascorbic acid 2-phosphate (AAP) to form ascorbic acid (AA) that further reduces Ag + and Cu 2+ to Ag NPs and Cu + , respectively. This method was highly sensitive and selective and could detect as low as 1 pg/mL of p24 antigen by naked eyes and had a good linearity in the concentration range 1–100 pg/mL. When using Ag + and Cu 2+ as media, the limit of detection (LOD) of the new method was 0.3 pg/mL and 0.2 pg/mL, respectively. Compared with clinical electrochemiluminescence immunoassay (ECLIA) results and clinical data, this method demonstrated good consistency for the quantification of HIV-1 p24 antigen in 34 clinical serum samples. In addition, this method could accurately distinguish HIV from other viruses and infections such as hepatitis B virus , systemic lupus erythematosus , hepatitis C virus , Epstein-Barr virus , cytomegalovirus , lipemia , and hemolysis . Therefore, our dual-mode analysis method may provide additional solutions to identify clinical HIV infection. Graphical abstract An immunofluorescence and visualization dual-mode strategy for the detection of p24 antigen was constructed based on immune recognition reaction and a phenomenon that cadmium telluride quantum dots (CdTe QDs) can selectively recognize Ag + /Ag nanoparticles (Ag NPs) and Cu 2+ /Cu + .
研究领域:
病毒学
·
免疫学
·
医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）