Characterization of the ABA receptor PYL gene family and identification of DoPYL1-interacting proteins in Dendrobium officinale
Background Abscisic acid (ABA) plays a crucial role in plant growth, development, and stress responses. The pyrabactin resistance-like (PYL) ABA receptor proteins, which are key components of ABA signal recognition and transmission. Dendrobium officinale is a precious traditional Chinese medicinal plant with known drought resistance. Nevertheless, the PYL genes of D. officinale, along with other orchid plants, remains unanalyzed.Results In this study, the PYL family was identified from D. officinale, consisting of 11 PYL proteins. A phylogenetic analysis showed that these DoPYL proteins were classified into three subfamilies. All the DoPYL proteins featured both Motif 1 and Motif 2. Notably, the members of subfamily I and III were intron-less, while those of subfamily II consistently displayed 2 introns. Analysis of expression patterns revealed that these DoPYL genes exhibit diverse spatiotemporal expression profiles in different organs. DoPYL1, DoPYL2, DoPYL4, DoPYL8 DoPYL9 and DoPYL10 followed a decreasing trend as protocorm-like bodies (PLBs) developed into plantlets. Under ABA and mannitol treatments, DoPYL1, DoPYL2, DoPYL4, DoPYL5, DoPYL6 and DoPYL10 exhibited significant down-regulation, while the expression of other genes remained unchanged. Following cold and salinity treatment, there was a notable up-regulation in the expression of the majority of DoPYL genes. PEG-simulated drought stress treatment led to the down-regulation of DoPYL genes. Furthermore, the DoPYL1 protein was localized in the cell membrane, cytoplasm and nucleus. During Yeast two-hybrid Library screening, we identified 40 potential interacting proteins of DoPYL1. Yeast two-hybrid verification confirmed that DoPYL1 interacted with DoRpb3, DoUbox, DoGrx, and DoATPsyn proteins.Conclusion Our results not only contribute to an understanding of the potential role of PYL proteins in growth, development and stress responses in Orchidaceae plants, but also provide additional theoretical guidance for breeding new varieties of D. officinale with resistance to abiotic stresses.
危险品化学品经营许可证（带存储）