ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2024
·
IF 10.8
Zeyu Cai,Chuanxin Ma,Yi Hao,Weili Jia,Yini Cao,Honghong Wu,Xinxin Xu,Lanfang Han,Chunyang Li,Heping Shang,Anqi Liang,Jason C. White,Baoshan Xing
Cerium dioxide nanoparticles (CeO2 NPs) have enzyme-like properties and scavenge excess ROS induced by stressors such as drought. However, the underlying molecular mechanisms by which CeO2 NPs enhance drought resistance are unknown. In this work, both foliar application and soil injection of CeO2 NPs were used to rice seedlings under a 30 day moderate drought (40% soil relative moisture). Foliar application of 4 mg of CeO2 NPs per pot reduced excess reactive oxygen species and abscisic acid (ABA) in rice leaves, thereby maintaining chloroplast structural integrity and photosynthetic output, ultimately increasing drought-stressed rice biomass by 31.3%. Genes associated with photosynthesis and ribosome activity provided the foundation by which CeO2 NPs enhanced rice drought resistance. Importantly, these genes were tightly regulated by ABA due to the large number of abscisic acid responsive elements in their promoter regions. CeO2 NPs also upregulated the expression of soluble sugar and fatty acid synthesis associated genes in drought-stressed rice, thereby contributing to osmotic balance and membrane lipid stability. These results highlight the potential of CeO2 NPs to enhance rice photosynthesis and drought-resistant biomolecule accumulation by regulating ABA-dependent responses. This work provides further evidence demonstrating nanomaterials have great potential to sustainably promote stress resistance and climate resilient crops.
研究领域:
植物学
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植物生理学
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分子生物学
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农学
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产品关联
DOI
PubMed
高影响因子
MACROMOLECULES
·
2020
·
IF 5.918
Qingbin He,Yanjie Zhang,Haolong Li,Quan Chen
In this study, we designed an ABA-type copolymer physically end-cross-linked by polyoxometalate and developed a versatile and robust pathway to tune its structural and rheological properties. The copolymer precursors are composed of a central poly(n-butyl acrylate) (nBA) block with DP ≅ 100 and two short vinyl imidazole (VIm) moieties as chain ends. Phase segregation occurs gradually for the precursors with an increase of the number of VIm per end, NVIm, from 1 to 7.6, and clear segregation is observed for precursor with the highest NVIm = 7.6, using small-angle X-ray scattering. For those precursors having NVIm ≤ 4.6, introduction of a small amount of silicotungstic acid, H4[SiW12O40], induces phase segregation, owing to a strong electrostatic attraction between [SiW12O40]4– ion and protonated VIm monomer. As a result, the copolymer chains are physically cross-linked, forming thermoplastic copolymers, for which the size of core, plateau modulus, and solid-to-liquid transition temperature can be tuned in wide ranges by the content of silicotungstic acid. The solid-to-liquid transition temperature is controlled by an energy barrier for dissociation of the VIm end moieties. Analysis of the solid-to-liquid transition temperature enables us to determine a scaling relationship between the energy barrier and the size and degree of protonation of the end moiety. The near-proportionality between the activation energy Ea and the length of the end moiety suggests a strong motional coupling of the ionic associations belonging to the same end moiety.
研究领域:
材料科学
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高分子化学
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产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2019
·
IF 3.571
Xueyuan Han,Linchun Mao,Wenjing Lu,Xiaopeng Wei,Tiejin Ying,Zisheng Luo
Wound-induced suberization is an essentially protective healing process for wounded fruit to reduce water loss and microbial infection. It has been demonstrated that abscisic acid (ABA) could promote wound suberization, but the molecular mechanism of ABA regulation remains little known. In this study, the transcript level of Achn030011 (designated as AchnKCS), coding a β-ketoacyl-coenzyme A synthase (KCS) involved in suberin biosynthesis, was found to be significantly upregulated by ABA in wounded kiwifruit. A bZIP transcription factor (Achn270881), a possible downstream transcription factor in the ABA signaling pathway, was screened and designated as AchnbZIP12 according to its homology with related Arabidopsis transcription factors. A yeast one-hybrid assay demonstrated that AchnbZIP12 could interact with the AchnKCS promoter. Furthermore, significant trans-activation of AchnbZIP12 on AchnKCS was verified. The transcript level of AchnbZIP12 was also upregulated upon treatment with ABA. These results imply that AchnbZIP12 acts as a positive regulator in ABA-mediated AchnKCS transcription during wound suberization of kiwifruit.
研究领域:
植物学
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分子生物学
✓ 已验证
DOI
PubMed
高影响因子
Canadian Journal of Chemistry
·
2017
·Vol. 95
·期 5
·
IF 1.08
Lei Wang,Shaoqing Wen,Zhanxiong Li
A series of novel amphiphilic ABA-type poly(tridecafluorooctylacrylate)-poly(ethylene glycol)-poly(tridecafluorooctylacrylate) (henceforth referred to as p-TDFA-PEG-p-TDFA) triblock oligomers were successfully synthesized via atom transfer radical polymerization (ATRP) using well-defined Br-PEG-Br as macroinitiator and copper as catalyst. The block oligomers were characterized by Fourier transform infrared (FTIR) spectroscopy and 1H and 19F nuclear magnetic resonances (NMR). Gel permeation chromatography (GPC) showed that the block oligomers have been obtained with narrow molecular weight distributions of 1.22–1.33. X-ray photoelectron spectroscopy (XPS) was carried out to confirm the attachment of p-TDFA-PEG-p-TDFA onto the silicon substrate, together with the chemical compositions of p-TDFA-PEG-p-TDFA. The wetabilities of the oligomer films were measured by water contact angles (CAs). Water CAs of p-TDFA-PEG-p-TDFA film were measured and their morphologies were tested by atomic force microscopy (AFM). The result showed that the CAs of the oligomer films, which possess fluoroalkyl groups assembled on the outer surface, increase after heating due to the migration of fluoroalkyl groups and the resulted microphase separation of the p-TDFA-PEG-p-TDFA.
研究领域:
材料科学
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表面化学
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DOI
高影响因子
BMC PLANT BIOLOGY
·
2025
·Vol. 25
·期 1
·1-14
·
IF 4.8
Chen Jing,Li Shoujie,Si Can,Liang Hanzhi,Duan Jun,He Chunmei
Background Abscisic acid (ABA) plays a crucial role in plant growth, development, and stress responses. The pyrabactin resistance-like (PYL) ABA receptor proteins, which are key components of ABA signal recognition and transmission. Dendrobium officinale is a precious traditional Chinese medicinal plant with known drought resistance. Nevertheless, the PYL genes of D. officinale, along with other orchid plants, remains unanalyzed.Results In this study, the PYL family was identified from D. officinale, consisting of 11 PYL proteins. A phylogenetic analysis showed that these DoPYL proteins were classified into three subfamilies. All the DoPYL proteins featured both Motif 1 and Motif 2. Notably, the members of subfamily I and III were intron-less, while those of subfamily II consistently displayed 2 introns. Analysis of expression patterns revealed that these DoPYL genes exhibit diverse spatiotemporal expression profiles in different organs. DoPYL1, DoPYL2, DoPYL4, DoPYL8 DoPYL9 and DoPYL10 followed a decreasing trend as protocorm-like bodies (PLBs) developed into plantlets. Under ABA and mannitol treatments, DoPYL1, DoPYL2, DoPYL4, DoPYL5, DoPYL6 and DoPYL10 exhibited significant down-regulation, while the expression of other genes remained unchanged. Following cold and salinity treatment, there was a notable up-regulation in the expression of the majority of DoPYL genes. PEG-simulated drought stress treatment led to the down-regulation of DoPYL genes. Furthermore, the DoPYL1 protein was localized in the cell membrane, cytoplasm and nucleus. During Yeast two-hybrid Library screening, we identified 40 potential interacting proteins of DoPYL1. Yeast two-hybrid verification confirmed that DoPYL1 interacted with DoRpb3, DoUbox, DoGrx, and DoATPsyn proteins.Conclusion Our results not only contribute to an understanding of the potential role of PYL proteins in growth, development and stress responses in Orchidaceae plants, but also provide additional theoretical guidance for breeding new varieties of D. officinale with resistance to abiotic stresses.
研究领域:
植物分子生物学
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药用植物学
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逆境生理学
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信号转导
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基因家族分析
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产品关联
DOI
PubMed
高影响因子
Microbial Cell Factories
·
2024
·Vol. 23
·期 1
·1-16
·
IF 4.3
Wang Yifan,Shu Dan,Li Zhemin,Luo Di,Yang Jie,Chen Dongbo,Li Tianfu,Hou Xiaonan,Yang Qi,Tan Hong
Background Currently, industrial fermentation of Botrytis cinerea is a significant source of abscisic acid (ABA). The crucial role of ABA in plants and its wide range of applications in agricultural production have resulted in the constant discovery of new derivatives and analogues. While modifying the ABA synthesis pathway of existing strains to produce ABA derivatives is a viable option, it is hindered by the limited synthesis capacity of these strains, which hinders further development and application. Results In this study, we knocked out the bcaba4 gene of B. cinerea TB-31 to obtain the 1′,4′- trans -ABA-diol producing strain ZX2. We then studied the fermentation broth of the batch-fed fermentation of the ZX2 strain using metabolomic analysis. The results showed significant accumulation of 3-hydroxy-3-methylglutaric acid, mevalonic acid, and mevalonolactone during the fermentation process, indicating potential rate-limiting steps in the 1′,4′- trans -ABA-diol synthesis pathway. This may be hindering the flow of the synthetic pathway. Additionally, analysis of the transcript levels of terpene synthesis pathway genes in this strain revealed a correlation between the bchmgr , bcerg12 , and bcaba1-3 genes and 1′,4′- trans -ABA-diol synthesis. To further increase the yield of 1′,4′- trans -ABA-diol, we constructed a pCBg418 plasmid suitable for the Agrobacterium tumefaciens -mediated transformation (ATMT) system and transformed it to obtain a single-gene overexpression strain. We found that overexpression of bchmgr , bcerg12 , bcaba1 , bcaba2 , and bcaba3 genes increased the yield of 1′,4′- trans -ABA-diol. The highest yielding ZX2 A3 strain was eventually screened, which produced a 1′,4′- trans -ABA-diol concentration of 7.96 mg/g DCW (54.4 mg/L) in 144 h of shake flask fermentation. This represents a 2.1-fold increase compared to the ZX2 strain. Conclusions We utilized metabolic engineering techniques to alter the ABA-synthesizing strain B. cinerea , resulting in the creation of the mutant strain ZX2, which has the ability to produce 1′,4′- trans -ABA-diol. By overexpressing the crucial genes involved in the 1′,4′- trans -ABA-diol synthesis pathway in ZX2, we observed a substantial increase in the production of 1′,4′- trans -ABA-diol.
研究领域:
生物技术
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微生物学
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代谢工程
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DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2019
·
IF 6.361
Haotian Shi,Teng Qiu,H. Daniel Ou-Yang,Huangbing Xu,Qingchuan Lu,Ying Zheng,Kexin Liu,Lifan He,Longhai Guo,Xiaoyu Li
A temperature sensitive sol-gel transition induced by the self-assembly of amphiphilic copolymers and its application in industry have been the objects of increasing study. We demonstrate here a two-step, reversible addition-fragmentation chain transfer (RAFT) polymerization of an ABA-type copolymer consisting of poly( N,N -dimethylacrylamide)- b -poly(diacetone acrylamide)- b -poly( N,N -dimethylacrylamide) (PDMAA- b -PDAAM- b -PDMAA). This copolymer can be easily dispersed in water, and this dispersion is critical for its lower critical solution temperature (LCST)-type sol-gel transition, which was monitored using dynamic light scattering (DLS), transmission electron microscopy (TEM), and rheology analysis, in addition to temperature-dependent 1 H nuclear magnetic resonance ( 1 H NMR) and Fourier transform infrared spectroscopy (FTIR). Results revealed an abnormal sphere-to-worm micellar transition of this ABA copolymer at the LCST point, which could be affected by the length of the PDAAM block (B-block), the length as well as the distribution of the PDMAA block (A-block), and the concentration of the copolymer dispersion. Thus, copolymer dispersion could be feasibly used for drug loading at a low temperature, which could then be transformed into a gel at an elevated temperature. The loading and controllable release of the model drug of paracetamol into and out of a copolymer gel was further determined. The sustained release behavior was also studied using the Rigter-Peppas model.
研究领域:
材料科学
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生物医学工程
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高分子化学
✓ 已验证
DOI
高影响因子
BMC PLANT BIOLOGY
·
2022
·Vol. 22
·期 1
·1-11
·
IF 5.26
Han Xueyuan,Wei Xiaopeng,Lu Wenjing,Wu Qiong,Mao Linchun,Luo Zisheng
Background Our previous study has demonstrated that the transcription of AchnKCS involved in suberin biosynthesis was up-regulated by exogenous abscisic acid (ABA) during the wound suberization of kiwifruit, but the regulatory mechanism has not been fully elucidated. Results Through subcellular localization analysis in this work, AchnbZIP29 and AchnMYB70 transcription factors were observed to be localized in the nucleus. Yeast one-hybrid and dual-luciferase assay proved the transcriptional activation of AchnMYB70 and transcriptional suppression of AchnbZIP29 on AchnKCS promoter. Furthermore, the transcription level of AchnMYB70 was enhanced by ABA during wound suberization of kiwifruit, but AchnbZIP29 transcription was reduced by ABA. Conclusions Therefore, it was believed that ABA enhanced the transcriptional activation of AchnMYB70 on AchnKCS by increasing AchnMYB70 expression. On the contrary, ABA relieved the inhibitory effect of AchnbZIP29 on transcription of AchnKCS by inhibiting AchnbZIP29 expression. These results gave further insight into the molecular regulatory network of ABA in wound suberization of kiwifruit.
研究领域:
植物生物学
·
分子遗传学
·
转录调控
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DOI
PubMed
高影响因子
RSC Advances
·
2014
·Vol. 4
·期 69
·36675-36681
·
IF 3.708
Jiangang Gao,Shaobo Yu,Benpei Zheng,Qingping Song,Xingxing Peng,Ying Lin,Gang Zou,Qijin Zhang
A triblock PEG-based polymer (CP1113) with a clear ABA structure was synthesized from 1,13-tridecanedioic acid (DA13) and mono-methoxy-poly(ethylene glycol) (MePEG) via condensation reaction. An inclusion complex (IC1113) with β-cyclodextrin (CD) was prepared by co-precipitation and the IC formation was confirmed by transmittance determination with a UV-Vis spectrometer. Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), and thermogravimetry analysis (TGA) were adopted to characterize IC1113 and the experimental data showed that a channel-type polypseudorotaxane was formed. For further investigating the inclusion behaviors, 1H-NMR spectroscopy was used to determine the molar ratio between CP1113 and β-CD and 2D ROSEY spectra were recorded to elucidate the IC formation mechanism. The results revealed that β-CD threaded onto the polymer main chain at a molar ratio of 7.8 : 1 and the molar ratio of EG segments to β-CD in IC1113 was ca. 8.28 : 1. It was concluded that β-CD accommodated both the methylene and the PEG segments and herein hydrophobic and hydrogen-bonds interactions played the most important roles with a hydrophobic stabilizing and hydrogen-bond inducing effect, which “amplified” the hydrophobic interaction between β-CD and the methylene segment along the hydrophilic PEG segments.
研究领域:
材料科学
·
化学
✓ 已验证
DOI
高影响因子
Nature Plants
·
2024
·
IF 15.8
Lin Ziwei,Guo Ying,Zhang Ruiyuan,Li Yiming,Wu Yue,Sheen Jen,Liu Kun-hsiang
Abscisic acid (ABA) regulates plant stress adaptation, growth and reproduction. Despite extensive ABA–Ca 2+ signalling links, imaging ABA-induced increases in Ca 2+ concentration has been challenging, except in guard cells. Here we visualize ABA-triggered [Ca 2+ ] dynamics in diverse organs and cell types of Arabidopsis thaliana using a genetically encoded Ca 2+ ratiometric sensor with a low-nanomolar Ca 2+ -binding affinity and a large dynamic range. The subcellular-targeted Ca 2+ ratiometric sensor reveals time-resolved and unique spatiotemporal Ca 2+ signatures from the initial plasma-membrane nanodomain, to cytosol, to nuclear oscillation. Via receptors and sucrose-non-fermenting1-related protein kinases (SnRK2.2/2.3/2.6), ABA activates low-nanomolar Ca 2+ transient and Ca 2+ -sensor protein kinase (CPK10/30/32) signalling in the root cap cycle from stem cells to cell detachment. Surprisingly, unlike the prevailing NaCl-stimulated micromolar Ca 2+ spike, salt stress induces a low-nanomolar Ca 2+ transient through ABA signalling, repressing key transcription factors that dictate cell fate and enzymes that are crucial to root cap maturation and slough. Our findings uncover ABA–Ca 2+ –CPK signalling that modulates root cap cycle plasticity in adaptation to adverse environments.
研究领域:
植物生物学
·
分子生物学
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细胞生物学
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信号传导
✓ 已验证
DOI
PubMed
高影响因子
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
·
2025
·
IF 2.2
Guixiang He,Feirong Zeng,Ge Song,Yuqiong Li,Pian Yang,Jihong Zhang,Yan Gao
CrRLK1L receptor kinases, particularly FERONIA ( FER ), play critical roles in plant stress adaptation and hormonal signaling, yet their functional characterization in economically vital crops like Brassica napus remains limited. Here, we identified 108 CrRLK1L family members through genome wide screening in B. napus and focused on BnFERONIA , a homolog evolutionarily conserved with FERONIA orthologs in Arabidopsis and related crucifers. CRISPR/Cas9 generated BnFERONIA knockout mutants ( cas9-fer ) in Arabidopsis exhibited contrasting abiotic stress responses: hypersensitivity to salt stress (100 mM NaCl), with root elongation was significantly inhibited compared to wild-type, and enhanced tolerance to osmotic stress (300 mM mannitol), displaying 20% longer roots. Furthermore, cas9-fer mutants showed pronounced ABA hypersensitivity, with germination rates significantly suppressed under 0.25 μM ABA and vegetative growth stunted, indicating the role of BnFERONIA in attenuating ABA signaling. Strikingly, osmotic-induced germination remained unaffected in mutants, suggesting there was a compensatory mechanisms during the early stress perception. This research provides the first functional evidence of BnFERONIA gene during stress adaptation, offering a molecular framework for creating stress-resistant oilseed crops by specifically manipulating the CrRLK1L pathways.
研究领域:
植物学
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植物科学
·
分子生物学
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遗传学
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农学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of CO2 Utilization
·
2024
·
IF 7.2
Yansong Ren,Tianyun Zhang,Shuanjin Wang,Dongmei Han,Sheng Huang,Hui Guo,Min Xiao,Yuezhong Meng
Although triblock polycarbonate thermoplastic elastomers (TPEs) have recently attracted great interests due to their biodegradability, insufficient attentions have been paid to improving the elastomeric properties. Through a tandem reaction strategy involving CO 2 / allyl glycidyl ether (AGE) / cyclohexene oxide (CHO), poly(cyclohexene carbonate)-b-poly(allyl glycidyl ether carbonate)-b-poly(cyclohexene carbonate) (PCAC) is successfully synthesized using a metal-free Lewis acid-base pair catalyst. The synthesized PCACs are pure well-defined ABA triblock all-polycarbonate, which is supported by 1 H NMR, gel permeation chromatography (GPC), and diffusion-ordered spectroscopy (DOSY). A simple modification by grafting alkylthiol chains to PCAC results in excellent TPEs named PCAC-g-S-C a H 2a+1 (PCASaC). The synthesized TPEs are characterized by atomic force microscopy (AFM), thermogravimetric analysis (TG), differential scanning calorimetry (DSC), and mechanical test. They demonstrate a semi-network and semi-domain phase separation structure, wide service temperature range, good strength, high elongation, and extremely high elastic recovery properties. This low-cost, biodegradable, high-performance TPE shows great potential for applications in biomedical, wearable products, sports equipment, and other fields.
研究领域:
材料科学
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聚合物化学
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可持续材料
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生物医学工程
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）