Natural Product Communications
·
2014
·
IF 0.924
Zihao Zhou,Jun Tang,Xueping Song
The chemical composition of the essential oil from roots and rhizomes of Ligularia hodgsonii was analyzed by gas chromatography (GC) and GC-mass spectrometry (MS). Forty-seven components were identified, representing 77.0% of the total oil. The main constituents were l-(+)-ascorbic acid 2,6-dihexadecanoate (15.7%), selina-6-en-4-ol (8.4%) and 9,10-dimethyl-1,2,3,4,5,6,7,8-octahydroanthracene (6.6%). The free radical scavenging activities of the essential oil, aqueous extract, ethanolic extract, and crude polysaccharides of L. hodgsonii, as well as some of their major components, were investigated using DPPH and ABTS assays. The essential oil displayed a lower capacity to quench free radicals than the extracts; the ethanolic and aqueous extracts showed considerably higher antioxidant potential that deserves further study.
研究领域:
植物化学
·
药理学
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DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2015
·
IF 4.169
L.X. Chen,D.J. Hu,S.C. Lam,L. Ge,D. Wu,J. Zhao,Z.R. Long,W.J. Yang,B. Fan,S.P. Li
Snow chrysanthemum ( Coreopsis tinctoria Nutt.), a world-widely well-known flower tea material, has attracted more and more attention because of its beneficial health effects such as antioxidant activity and special flavor. In this study, a high performance liquid chromatography coupled with diode array detection and mass spectrometry (HPLC-DAD-MS) and 2,2′-azinobis(3-ethylbenzthiazoline-sulfonic acid)diammonium salt (ABTS) based assay was employed for comparison and identification of antioxidants in different samples of snow chrysanthemum. The results showed that snow chrysanthemum flowers possessed the highest while stems presented the lowest antioxidant capacities. Fourteen detected peaks with antioxidant activity were temporarily identified as 3,4′,5,6,7-pentahydroxyflavanone-O-hexoside, chlorogenic acid, 2R-3′,4′,8-trihydroxyflavanone-7-O-glucoside, flavanomarein, flavanocorepsin, flavanokanin, quercetagitin-7-O-glucoside, 3′,5,5′,7-tetrahydroxyflavanone-O-hexoside, marein, maritimein, 1,3-dicaffeoylquinic acid, coreopsin, okanin and acetyl-marein by comparing their UV spectra, retention times and MS data with standards or literature data. Antioxidants existed in snow chrysanthemum are quite different from those reported in Chrysanthemum morifolium , a well-known traditional beverage in China, which indicated that snow chrysanthemum may be a promising herbal tea material with obvious antioxidant activity.
研究领域:
植物化学
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食品科学
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药用植物研究
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DOI
高影响因子
CHEMISTRY OF NATURAL COMPOUNDS
·
2020
·Vol. 56
·期 5
·821-826
·
IF 0.653
Shao Jin,Zhao Tong,Ma Hui-Ping,Jia Zheng-Ping,Jing Lin-Lin
A convergent synthesis route of 6-hydroxygenistein (6-OHG) was reported, starting from cheap and readily available biochanin A, via methylation, bromination, methoxylation, and demethylation. The structure of the products was confirmed by MS, IR, 1 H NMR, and 13 C NMR analysis. The antiradical activity of 6-OHG was determined using six different methods, namely, DPPH assay, ABTS assay, nitric oxide assay, superoxide assay, reducing power assay, and phosphomolybdenum assay using ascorbic acid (V C ) as positive control. The results show that 6-OHG possesses similar or greater antiradical activity than V C . In conclusion, 6-OHG can be useful as an antioxidant agent.
研究领域:
有机化学
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药物化学
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天然产物化学
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DOI
高影响因子
Natural Product Communications
·
2015
·
IF 0.906
Jian-Wei Dong,Le Cai,Yun Xing,Jing Yu,Zhong-Tao Ding
2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radical cation (ABTS•+) is a stable free radical frequently used for estimating the total antioxidant capacity (TAC) of natural products. The existing methods for ABTS•+ radical-scavenging activity assays are diverse in pre-diluting solvents and reaction time, which lead to errors in the TAC estimations. To develop an effective and universal method for estimating the ABTS•+ capacity accurately and reasonably, five pre-dilution solvents [methanol, ethanol, phosphate buffer (Na2HPO4-NaH2PO4, 200 mM, pH = 7.4), PBS buffer (Na2HPO4-NaH2PO4-NaCl, 200 mM, pH = 7.4), and distilled water] and different reaction times were investigated in ABTS•+ assays of five typical antioxidants. The results showed that the solvent effects were very significant. When using different pre-diluting solvents, different detection wavelengths should be selected. ABTS-+ assay could be measured within 2–10 min to obtain a rough result, which was mostly 6 min in the literature. However, full and accurate evaluation of antioxidant reactivity rather than capacity requires recording ABTS•+ loss continuously during the whole reaction period. The present study makes a recommendation for estimating the ABTS•+ capacity accurately and reasonably.
研究领域:
生物化学
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分析化学
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DOI
高影响因子
JOURNAL OF FOOD SCIENCE
·
2015
·Vol. 80
·期 6
·C1162
·
IF 1.696
Hai-wei Jiang,Hong-yan Li*,Cheng-wei Yu,Ting-ting Yang,Jiang-ning Hu,Rong Liu,Ze-Yuan Deng*
en Isobolographic analysis was used to assess the antioxidant interactions (synergism, addition, and antagonism) of 4 common vegetables (tomato [T], carrot [C], eggplant [E], and purple potato [P]). The lipophilic (L) extracts of T and C (main carotenoids), the hydrophilic (H) extracts of E and P (main phenolics) were mixed by the certain ratios (1:9, 3:7, 1:1, 7:3, 9:1, w/w) and their antioxidant activities were investigated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2’-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) radical scavenging assays, respectively. Most of the binary mixtures (LC-HE, LC-HP, HE-HP, LT-HE, and LT-HP combinations) showed the synergistic antioxidant effects. In DPPH assay, the greatest antioxidant activity of vegetable combinations was 1:9 LT-HP (EC 50 : 2.45 ± 0.13 mg/mL), followed by 9:1 HE-HP (EC 50 : 3.62 ± 0.12 mg/mL) and 1:9 LC-HE (EC 50 : 3.74 ± 0.47 mg/mL). In ABTS assay, the greatest antioxidant activity of vegetable combinations was 9:1 HE-HP (EC 50 : 4.20 ± 0.10 mg/mL), followed by 7:3 HE-HP (EC 50 : 4.41 ± 0.63 mg/mL) and 1:1 HE-HP (EC 50 : 5.35 ± 0.85 mg/mL). Among these combinations, 1:1 LC-HE combination showed the highest synergistic antioxidant effects in DPPH assay (synergistic rate: 87.4%), and 7:3 LC-HE combination showed the highest synergistic antioxidant effects in ABTS assay (synergistic rate: 87.0%). The mixtures of phenolics and carotenoids with suitable ratios in vegetables effectively enhanced the synergistic antioxidant effects. Practical Application pt In this study, the results indicate that the combination of different vegetables with suitable ratios could improve the antioxidant activities through synergistic effects of phytochemicals. Also, isobolographic analysis could be well used in evaluating the antioxidant interactions between antioxidants.
研究领域:
食品科学
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营养学
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生物化学
·
植物化学
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产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2017
·Vol. 22
·期 1
·171
·
IF 2.861
Xiangcong Wang,Maocai Yan,Qibao Wang,Huannan Wang,Zhengyang Wang,Jiayi Zhao,Jing Li,Zhen Zhang
Indole-2-carboxylic acid copper complex (ICA-Cu) was successfully prepared and characterized through elemental analysis, IR, UV-Vis,1H-NMR, TG analysis, and molar conductance, and its molecular formula was [Cu2(C9H6O2N)4(H2O)2]·2H2O. The binding ability of ICA-Cu to calf thymus DNA (CT-DNA) was examined by fluorescence spectrometry and the viscosity method. The results indicated that, upon the addition of increasing amounts of CT-DNA, the excitation and emission intensity of ICA-Cu decreased obviously and the excitation spectra shifted towards a long wavelength. ICA-Cu could displace ethidium bromide (EB) from the EB-DNA system, making the fluorescence intensity of the EB-DNA system decrease sharply; the quenching constantKSVvalue was 3.99 × 104M−1. The emission intensity of the ICA-Cu-DNA system was nearly constant, along with the addition of Na+in a series of concentrations. The fluorescence of the complex could be protected after the complex interacted with DNA. A viscosity measurement further supported the result that the ICA-Cu complex may interact with DNA in an intercalative binding mode. The antioxidant activities of ICA-Cu were evaluated by a 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, a hydroxyl radical (OH) scavenging assay, and a 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) assay. The ICA-Cu exhibited the highest inhibitory effects on the ABTS radical (94% inhibition at 60 µM), followed by OH and DPPH radicals (the degrees of inhibition being 71% and 56%, respectively). The in vitro cytotoxicity activity of ICA-Cu against two human breast cancer cell lines, MDA-MB-231 and MCF-7, was investigated by 3-[4,5-dimethyltiazol2-yl]-2.5-diphenyl-tetrazolium bromide (MTT) assay and cellular morphological analysis. The results showed that, upon increasing the concentration of ICA-Cu, an increase was observed in growth-inhibitory activity and the inhibition percentage were greater than 90% at 20 µM in both cell lines. Also, cellular morphological changes in the two cell lines agreed with the cytotoxicity results.Keywords:DNA-binding;antioxidant;anticancer;indole-2-carboxylicacid;copper(II) complexes
研究领域:
化学
·
生物化学
·
药理学
·
癌症研究
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产品关联
DOI
PubMed
高影响因子
Biomass Conversion and Biorefinery
·
2022
·
IF 4.05
Shang Xian-chao,Zhang Yu-qin,Zheng Yan-fen,Li Yiqiang
Polyphenols and flavonoids are abundant in spent onion skins (SOSs), being present in amounts are 2–sixfold greater times of than those in the inner bulb. In this study, an eco-friendly and efficient extraction method based on temperature-responsive deep eutectic solvents (TRDESs) was used to obtain natural flavonoids from SOSs. Microwave-assisted extraction yields of quercetin, kaempferol, luteolin, and quercetin-3-O-β-D-glucoside were evaluated to determine the efficiencies of a series of TRDESs. An extraction system composed of ethanolamine and m -cresol at ratio of 1:1 (20% water in TRDES system) was found to possess the highest efficiency. The extraction conditions were screened and optimized for efficiency using a Box-Behnken design. As a result, an extraction power of 554 W, extraction temperature of 76 °C, extraction time of 16 min, and solid–liquid ratio of 14 mg/mL were determined to be the optimal conditions. Under these extraction conditions, the total flavonoid extraction yields reached 47.83 ± 0.21 mg/g. The macroporous resin HPD 600 was selected for the separation and recovery of the TRDESs extraction mixture and the experimental measurements revealed that it allowed recovery rate more than 90%. In vitro activity testing of the flavonoid extract demonstrated excellent FRAP assay, DPPH assay and ABTS assay results for the SOSs extract prepared using a TRDES, and its inhibitory effects on S. aureus , E. carotovora , and B. subtilis were marked.
研究领域:
化学工程
·
环境科学
·
药学
·
食品科学
·
分析化学
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产品关联
DOI
高影响因子
JOURNAL OF FOOD ENGINEERING
·
2022
·
IF 6.203
Qingyin Dai,Xi Huang,Ruijing Jia,Yanyuan Fang,Zhiyong Qin
An antimicrobial composite film based on sodium alginate (SA)/cellulose nanofibers (CNFs) containing peanut red skin extract (PSE) was prepared by crosslinking with Ca 2+ for the purpose of fruit preservation. The bioactive chemical constituents present in PSE were investigated using liquid chromatography–mass spectrometry (LC–MS). The SA/CNFs/Ca 2+ /PSE (SCCP) film exhibited high strength, good water resistance, and excellent ultraviolet barrier performance. The release behavior and antioxidant and antibacterial activity of the SCCP film were investigated, and its applicability to fruit preservation was evaluated. Higher radical scavenging activity of the film was observed in the ABTS assay radical compared with the DPPH assay, especially in 10 % ethanol; the maximum ABTS scavenging activity was 99.28 %. For surfaces covered by the SCCP film, the rates of fruit decay and weight loss were lower than in the control group. Furthermore, the films exhibited significant antibacterial effects against both gram-negative and gram-positive bacteria.
研究领域:
食品科学
·
材料科学
·
生物技术
·
化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）