FITOTERAPIA
·
2019
·
IF 2.431
Fenqin Zhao,Qianqian Zhang,Yan Yan,Haiyan Jia,Xinran Zhao,Xiying Li,Lihua Zheng,Guang Han
Dendranthema morifolium cv. ‘jinsidaju’, cultivated only in Kaifeng, has been eaten for more than 1000 years. During the antioxidant-activity-guided studies on its chemistry and health care function, two new bisabolane-type sesquiterpenes , (6 R ,7 R )-7-hydroxybisabol-2,9 E ,11-triene-4-one (jinsidajuol A, 1 ) and (6 R ,7 R )-7-hydroxy-11-methoxybisabol-2,9 E -diene-4-one (jinsidajuol B, 2 ), and thirteen known compounds ( 3 – 15 ) were isolated from the flowers. Their structures were elucidated by 1D and 2D NMR spectroscopy and HRMS. 1 and 2 are the first example of bisabolane-type sesquiterpenes isolated from the genus Dendranthema. Compounds 6 – 8 , 12 and 13 exhibited strong scavenging activities on the ABTS radical cation with IC 50 3.33, 5.67, 2.00, 2.50, 5.33 μg/mL, respectively. The IC 50 values of all compounds on HepG2 human hepatoma tumor cell line were higher than 50 μM.
研究领域:
药用植物学
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生物化学
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植物化学
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DOI
PubMed
高影响因子
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
·
2023
·
IF 2.9
Dongyu Gu,Shanshan Tang,Chang Liu,Dajun He,Jing Tian,Yi Yang
Coprinus comatus is an edible and medicinal fungus. In this study, the antioxidant activity of the fermentation product of C. comatus was investigated through optimization of fermentation process. The results indicated that the fermentation product of C. comatus had obvious scavenging ability for 2,2’-Azino-bis(3-ethylbenzothiazoline)-6-sulphonic acid (ABTS) free radical. The EC50 of the n-butanol extract from the fermentation product on ABTS·+ was 0.65 ± 0.02 mg/mL. On this basis, the liquid fermentation conditions of C. comatus were optimized through single factor and response surface optimization experiments according to the scavenging ability of ABTS·+ to improve the antioxidant capacity of the fermentation product. The results showed that when the 14% of C. comatus was fermented in a culture medium with a C/N ratio of 48:1 for 6 days, the ABTS·+ scavenging ability was the strongest, and the EC50 of n-butanol extract was 0.57 ± 0.01 mg/mL, which was 12.31% higher than the initial activity. This study laid the foundation for the development of C. comatus.
研究领域:
微生物学
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食品科学
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生物技术
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DOI
PubMed
高影响因子
Foods
·
2024
·Vol. 13
·期 2
·230
·
IF 4.7
Hua Li,Yurong Mao,Danni Ma,Hua Li,Ruixin Liu,Sirithon Siriamornpun
Water boiling under atmospheric pressure (CAP) and water boiling under high pressure (CHP) are two popular domestic cooking methods for Chinese porridge making. In this study, we aimed to evaluate the effects of these two methods on the phenolic acid composition, antioxidant activity, and starch digestibility of triticale porridges. The contents of total free and total bound phenolic acids in the CHP sample were 1.3 and 1.6 times higher than those in the CAP counterpart, respectively, although the DPPH and ABTS values of these two samples were comparable. CAP induced more small pieces of starch than CHP, and the gelatinization enthalpy was 19% higher in the CHP sample than that in the CAP. Both cooking methods increased the starch digestibility, while the CHP sample (58.84) showed a lower GI than the CAP (61.52). These results may promote the application of triticale in health-promoting staple foods.Keywords:phenolic acid;DPPH radical scavenging ability;ABTS radical scavenging ability;resistant starch;starch digestion;glycemic index;cereal
研究领域:
食品科学
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营养学
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化学
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DOI
PubMed
高影响因子
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
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2015
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IF 0.476
Yaping Li,Lili Wang,Yanbei Tu,Jin Yan,Kailin Xu,Hui Li
Emodin (EM), with two p K a values (p K a 1 = 8.0 and p K a 2 = 10.9), presents low solubility and dissolution rate, which results in low bioavailability. In this report, two EM sodium salts, C 15 H 9 O 5 − · N a + (EM-1Na) and C 15 H 8 O 5 2− · 2 N a + (EM-2Na), were prepared by adding various amounts of sodium hydroxide . Salt forms were characterized by FT-IR, XRPD, 1 H NMR, DSC , and SEM, as well as solubility and dissolution rate studies in triple-deionized water, buffer at pH 1.2 and pH 6.8, respectively. The aqueous solubility of EM was notably enhanced by more than 20,000 and 50,000 times, corresponding to EM-1Na and EM-2Na. Meanwhile, the dissolution rate exhibited significant enhancement and the dissolution time was shortened. The results showed the dissolution of EM-1Na and EM-2Na was 25 and 35 times greater than EM in 60 min. The results of β-amyloid (Aβ) self-aggregation inhibition and MIC assay did not show apparent difference from those of EM, indicating that the functional groups were not broken down. Moreover, salt formation significantly enhanced the bioavailability of EM, consistent with the ABTS free radical scavenging test results. The significant decrease in IC 50 values in the range of 18,394 μg/mL to 29.07 μg/mL indicated a more effective anti-AD action.
研究领域:
药剂学
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药物化学
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药物递送
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神经科学
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微生物学
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DOI
高影响因子
TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH
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2012
·Vol. 11
·期 4
·545-551
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IF 0.82
E Yuan,B Liu,W Li,Q Li
Purpose: To improve the aqueous solubility of phloridzin by complexing it with hydroxypropyl-β-cyclodextrin (HP-β-CD).Methods: The complex of phloridzin with HP-β-CD was prepared by freeze-drying method. The physicochemical properties of the complex were investigated by ultraviolet-visible spectrometry (UV), infrared spectrometry (IR), differential scanning calorimetry (DSC) and x-ray diffractometry (XRD). The antioxidant activity was examined by DPPH and ABTS radical-scavenging activities.Results: Phloridzin in the complex was molecularly dispersed in HP-β-CD matrix. The complex was an effective scavenger of DPPH and ABTS radicals. At a concentration of 0.8 mg/mL and 30 μg/mL, DPPH and ABTS radical scavenging activities of the complex were 83.7 and 74.9 %, respectively.Conclusion: By forming inclusion complex with HP-β-CD, the solubility of phloridzin in water was significantly enhanced. The complex showed strong DPPH and ABTS radical scavenging activities.
研究领域:
药剂学
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药物化学
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分子药理学
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天然产物化学
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药物递送系统
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抗氧化研究
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DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2022
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IF 6.056
Yan Xu,Jing Zhang,Teng Pan,Fazheng Ren,Hailing Luo,Hao Zhang
Chlorogenic acid (CGA), a hydrophilic polyphenolic compound, is difficult to use in lipophilic systems. Accordingly, to improve its lipophilicity and explore the effect of alkyl chain unsaturation on antioxidant activity, CGA was acylated with fatty acids of the same chain length, but an increasing degree of unsaturation, namely, stearic acid [C18:0], oleic acid [C18:1], linoleic acid [C18:2], and linolenic acid [C18:3]. The major products were monoesters, as confirmed by ESI-MS, 1 H NMR, 13 C NMR, and Fourier transform infrared spectra (FT-IR) analysis. The lipophilicity of CGA derivatives was enhanced by acylation, and decreased with an increasing degree of fatty acid unsaturation. All CGA derivatives showed a high level of antioxidant activity. In detail, CGA-C18:0, CGA-C18:1, and CGA-C18:2 showed similar antioxidant activities, while CGA-C18:3 had the lowest antioxidant activity. The DPPH radical and ABTS radical cation scavenging activities of CGA-C18:3 were equivalent to 66.9% and 62.0% of those of CGA, respectively. The results of peroxide value (PV) indicated that the oxidative stability of CGA derivatives was enhanced. These results indicated that CGA derivatives could serve as potential sources of antioxidants for lipophilic food systems.
研究领域:
食品化学
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药学
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有机化学
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产品关联
DOI
高影响因子
Food Bioscience
·
2023
·
IF 5.2
Hongchi Zhou,Sixuan Wang,Weichao Liu,Lixuan Chang,Xuemei Zhu,Guangqing Mu,Fang Qian
This study aimed to investigate the safety and probiotic properties of Lactobacillus paraplantarum LS-5 isolated from traditional northeastern sauerkraut , and to assess the antioxidant capacity of different solvent extracts (ethanol, water, and hot water) of L. paraplantarum LS-5-inoculated sauerkraut. L. paraplantarum LS-5 was shown to have various probiotic characteristics, such as good survival ability under artificial gastrointestinal conditions, antagonistic activity of various pathogens, non-biogenic amines, antibiotic susceptibility , and non-hemolytic. The 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonate (ABTS) radical scavenging activity of the L. paraplantarum LS-5-inoculated sauerkraut alcohol extract (71.13%) was significantly higher than water extract (59.59%) ( p < 0.05). The ferric reducing antioxidant power (FRAP) of sauerkraut alcohol extract (614.93 Trolox μmol L −1 ) exhibited a slightly stronger ability compared to water extract (388.53 Trolox μmol L −1 ) and the heated sauerkraut water extract (383.20 Trolox μmol L −1 ) at extract concentrations of 5.0 mg mL −1 . Its 1, 1-diphenyl1-2-picrylhydrazyl (DPPH) radical scavenging activity was also stronger. It was found by principal component analysis (PCA) and Pearson that the DPPH radical-scavenging activities of sauerkraut extracts were significantly correlated with the content of polyphenols and flavonoids ( p < 0.05). Unlike kimchi , Northeastern sauerkraut is consumed mainly by heating, so this study could contribute to understanding the antioxidant capacity of different solvent extracts of sauerkraut, and before and after heating water extracts.
研究领域:
微生物学
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食品科学
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营养学
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生物化学
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产品关联
DOI
高影响因子
FITOTERAPIA
·
2023
·
IF 3.4
Yang Liu,Limei Ran,Yahong Wang,Peng Wan,Hongli Zhou
The polysaccharides from Sea buckthorn leaves (SBLPs) were extracted by hot water and purified by DEAE cellulose , then separated into six polysaccharides (SBLP-S) by DEAE-52 column. Six separated polysaccharides were characterized by Ultraviolet Spectroscopy, Infrared Spectrum, High Performance Liquid Chromatographic and Congo red analysis. The antioxidant activity and immunological activity were investigated in vitro . The results revealed that the monosaccharide composition of SBLP-S-1, SBLP-S-2, SBLP-S-3, SBLP-S-5 and SBLP-S-6 contained Man , GlcN , Rib , Rha , GluA , GalA , Glu, Gal, Xyl , Ara and Fuc , among them, rare glucosamine was found. And SBLP-S-4 contained all above components except GlcN and GluA. FT-IR showed that SBLP-S were sulfated polysaccharide containing uronic acid . Molecular weights of SBLP-S were 338.659, 401.305, 599.849, 393.904, 626.895 and 176.862 kDa. The Congo-red test indicated that SBLP-S-2, SBLP-S-4, SBLP-S-5, and SBLP-S-6 had triple helix conformation. Crude polysaccharides had the strong scavenging activities on DPPH radicals, ABTS radicals and hydroxyl radicals . The six polysaccharides had the activity of immune stimulation on RAW264.7 cell. SBLP-S-2 promoted the phagocytosis best and SBLP-S-6 promoted the NO production best. The results suggested that SBLPs could be used as potential antioxidants and immunomodulatory agents in pharmaceutical and functional food fields.
研究领域:
药理学
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食品科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHYSICAL CHEMISTRY B
·
2019
·
IF 2.923
Yi Xu,Jing Yang,Yao Lu,Ling-Ling Qian,Zhi-Yin Yang,Rui-Min Han,Jian-Ping Zhang,Leif H. Skibsted
Luteolin differs as a radical scavenger dramatically from apigenin in response to Cu(II) coordination despite a minor structural difference. Coordination of Cu(II) increases the radical scavenging efficiency of luteolin, especially at low pH, while decreases the efficiency of apigenin at both low and higher pH as studied by ABTS•+ radical scavenging. Luteolin forms a 1:1 complex with Cu(II) binding to 4-carbonyl and 5-phenol for pH <6 and to 3′,4′-catechol for pH >6. Apigenin forms a 1:2 complex independent of pH coordinated to 4-carbonyl and 5-hydroxylyl. Cu(II) coordinated to luteolin, as studied by pH jump stopped-flow, translocates with rate constants of 11.1 ± 0.3 s–1 from 4,5 to 3′,4′ sites and 1.0 ± 0.1 s–1 from 3′,4′ to 4,5 sites independent of Cu(II) concentration, pointing toward the dissociation of Cu(II) from an intermediate with two Cu(II) coordination as rate determining. 3′,4′-Catechol is suggested to be a switch for Cu(II) translocation with deprotonation initiating 4,5 to 3′,4′ translocation and protonation initiating 3′,4′ to 4,5 translocation. For dicoordinated apigenin, the coordination symmetry balances an electron withdrawal effect of Cu(II) resulting in a decrease of phenol acidity and less radical scavenging efficiency compared to parent apigenin. Compared to that of parent luteolin, the radical scavenging rate of both 4,5 and 3′,4′ Cu(II)-coordinated luteolin is enhanced through increased phenol acidity by electron withdrawal by Cu(II), as confirmed by density functional theory (DFT) calculations. Coordination and translocation of Cu(II) accordingly increases the antioxidant activity of luteolin at pH approaching the physiological level and is discovered as a novel class of natural molecular machinery derived from plant polyphenols, which seems to be of importance for protection against oxidative stress.
研究领域:
化学
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生物化学
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药理学
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植物科学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2016
·
IF 4.052
Qian Li,Xieyi Wang,Jun Chen,Chengmei Liu,Ti Li,David Julian McClements,Taotao Dai,Jiyan Liu
An extract isolated from Choerospondias axillaris peels was separated into five fractions using size-exclusion chromatography. The structural composition and mean degree of polymerization (mDP) of these fractions were then characterized by acid-catalysis followed by HPLC analysis. The antioxidant activity of each fraction was determined using a combination of chemical-based methods (DPPH , ABTS + radical scavenging activity, ferric-reducing antioxidant power, and phosphomolybdate assay) and a cellular-based assay. All fractions tested were found to have high total phenolics contents and were rich in proanthocyanidins. The mDP of fractions (F1–F5) ranged from 1.92 to 9.25. When tested by the chemical-based assays, the antioxidant activity of the fractions did not depend on molecular weight of the phenolics. Conversely, when tested by the cellular-based assay the antioxidant activity actually decreased with increasing molecular weight of the proanthocyanidins. These experiments highlight the limitations of using chemical-based assays to establish the antioxidant activity of proanthocyanidins within biological systems.
研究领域:
药理学
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生物化学
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食品科学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Xiao Yu,Yanan Chen,Zhiguo Qi,Quan Chen,Yujin Cao,Qingshan Kong
The antioxidant activity of corn peptides is related to their molecular weight and structure. Corn gluten meal (CGM) was hydrolyzed using a combination of Alcalase, Flavorzyme and Protamex, and the hydrolysates were subjected to antioxidant activity analysis after further fractionation. Corn peptides with molecular weights less than 1 kDa (CPP1) exhibited excellent antioxidant activity. A novel peptide, Arg-Tyr-Leu-Leu (RYLL), was identified from CPP1. RYLL displayed preferable scavenging capacities for ABTS radicals and DPPH radicals, with IC 50 values of 0.122 mg/ml and 0.180 mg/ml, respectively. Based on quantum calculations, RYLL had multiple antioxidant active sites, and tyrosine was the main active site due to the highest energy of the highest occupied molecular orbit (HOMO). Moreover, the simple peptide structure and hydrogen bond network of RYLL contributed to the exposure of the active site. This study elucidated the antioxidant mechanism of corn peptides, which could provide an understanding for CGM hydrolysates as natural antioxidants.
研究领域:
食品科学
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生物化学
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药学化学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Nutrition
·
2022
·
IF 6.59
Luo Bi,Lv Jia,Li Kejie,Liao Peiran,Chen Peng
This study aimed to extract polysaccharides from Citrus medica L. var. sarcodactylis (finger citron fruits) and analyze their structures and potential bioactivities. A new polysaccharide named K-CMLP was isolated and purified by Diethylaminoethylcellulose (DEAE)-Sepharose Fast Flow and DEAE-52 cellulose column chromatography with an average molecular weight of 3.76 × 103 kDa. Monosaccharide composition analysis revealed that K-CLMP consisted of rhamnose, galactose, and glucose, with a molar ratio of 6.75:5.87:1.00. Co-resolved by methylation and two-dimensional nuclear magnetic resonance (NMR), K-CLMP was alternately connected with 1, 2-Rha and 1, 4-Gal to form the backbone, and a small number of glucose residues was connected to O-4 of rhamnose. The results of DPPH⋅ and ABTS+⋅ radical scavenging assays indicated that both crude polysaccharide Citrus medica L. var. polysaccharide (CMLP) and K-CLMP exhibited strong free-radical-scavenging properties in a dose-dependent manner. In addition, K-CMLP significantly inhibited the production of pro-inflammatory cytokines (IL-6 and TNF-α) and reactive oxygen species (ROS) in RAW 264.7 cells treated with LPS. These results provide a basis for further use as one of the potential functions of food or natural medicine.
研究领域:
药理学
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生物化学
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食品科学
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植物科学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）