Biomolecules
·
2024
·Vol. 14
·期 5
·581
·
IF 4.8
Jiaming Cao,Boyuan Xiang,Baojie Dou,Jingfei Hu,Lei Zhang,Xinxin Kang,Mingsheng Lyu,Shujun Wang
Peptides possessing antihypertensive attributes via inhibiting the angiotensin-converting enzyme (ACE) were derived through the enzymatic degradation ofTrichiurus lepturus(ribbonfish) using alkaline protease. The resulting mixture underwent filtration using centrifugation, ultrafiltration tubes, and Sephadex G-25 gels. Peptides exhibiting ACE-inhibitory properties and DPPH free-radical-scavenging abilities were isolated and subsequently purified via LC/MS-MS, leading to the identification of over 100 peptide components. In silico screening yielded five ACE inhibitory peptides: FAGDDAPR, QGPIGPR, IFPRNPP, AGFAGDDAPR, and GPTGPAGPR. Among these, IFPRNPP and AGFAGDDAPR were found to be allergenic, while FAGDDAPRR, QGPIGPR, and GPTGPAGP showed good ACE-inhibitory effects. IC50values for the latter peptides were obtained from HUVEC cells: FAGDDAPRR (IC50= 262.98 μM), QGPIGPR (IC50= 81.09 μM), and GPTGPAGP (IC50= 168.11 μM). Peptide constituents derived from ribbonfish proteins effectively modulated ACE activity, thus underscoring their therapeutic potential. Molecular docking and modeling corroborated these findings, emphasizing the utility of functional foods as a promising avenue for the treatment and prevention of hypertension, with potential ancillary health benefits and applications as substitutes for synthetic drugs.
研究领域:
食品科学
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生物化学
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药理学
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产品关联
DOI
PubMed
高影响因子
Food & Function
·
2018
·Vol. 9
·期 4
·2270-2281
·
IF 3.289
Han Wu,Xin Rui,Wei Li,Yu Xiao,Jianzhong Zhou,Mingsheng Dong
This study explored a novel strategy to develop solid-state whole-grain oats as a novel carrier of lactic acid bacteria and a nutraceutical supplement rich in ACE inhibitory peptides. Oats were fermented by Lactobacillus plantarum B1-6, Rhizopus oryzae, or a combination of L. plantarum B1-6 and R. oryzae. L. plantarum showed a much better growth performance in oats when it was combined with R. oryzae than when it was cultured alone, as evidenced by an increase in viable cell count to 9.70 log cfu g−1 after 72 h of fermentation. The coinoculated fermented oats (CFO) and the R. oryzae-fermented oats (RFO) were then selected for investigations on protein hydrolysis and on the functional properties of the released bioactive peptides. The results showed that the soluble protein contents changed from 7.05 mg g−1 to 14.43 and 10.21 mg g−1 for CFO and RFO, respectively. However, the degree of hydrolysis and the content of peptides with molecular masses less than 10 000 Da indicated that the CFO proteins can be degraded to a greater degree. As analyzed by electrophoresis and reversed-phase high-performance liquid chromatography, the protein and peptide profiles of CFO and RFO demonstrated that the proteins from CFO were more obviously hydrolyzed and more small peptides were obtained. In addition, both CFO and RFO presented higher ACE inhibitory activities than unfermented oats, whereas the protein extracts from CFO exerted a lower IC50 value of 0.42 mg protein per mL compared with the protein extracts from the other samples. This research has broadened our knowledge on the development of whole-grain oat products as a probiotic carrier and on the difference between mixed solid-state fermentation (SSF) and fungi SSF in terms of protein degradation and the capacity to release ACE inhibitory peptides. Our approach could be used to obtain probiotic food products and probably to develop oats as a potential therapeutic ingredient targeting hypertension.
研究领域:
食品科学
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微生物学
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营养学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Heyong Wang,Meiluan Wang,Guixiang Huang,Mingye Zhao,Yongzhen Peng,Ning Zhu,Zheng Fang,Yujing Hu,Kai Guo
Optically active ethyl ( R )-2-hydroxy-4-phenylbutanoate (( R )-HPBE), is a key component in the production of angiotensin-converting enzyme (ACE) inhibitors, which are essential for treating hypertension. However, the high cost of starting materials has been a barrier to the industrial synthesis of ( R )-HPBE. Herein, we have established for the first time a four-step enzymatic reaction in continuous flow for the efficient synthesis of ( R )-HPBE from inexpensive benzaldehyde and pyruvic acid. This route also overcomes the issues of chemical disequilibrium and incompatibility of reaction conditions that are common in multi-enzyme cascades. The immobilization methods for aldolase/ene-reductase/keto-reductase were developed, and the immobilized enzymes were initially used in three individual flow modules to produce ( R )-2-hydroxy-4-phenylbutanoic acid (( R )-HPB), achieving high space–time yields of 3103 g (OPBEA) L −1 d −1 , 1685 g (OPBE) L −1 d −1 and 1709 g ( R )-HPB L −1 d −1 respectively. Moreover, integrating three modules in continuous flow achieved a space–time yield of about 350 g ( R )-HPB L −1 d −1 after 108 h, and maintained high operational stability with a conversion of >99 % and a product ee of >99.9 % after 192 h. Subsequently, the ethyl esterification of ( R )-HPB as the fourth module for producing ( R )-HPBE obtained a high space–time yield of 2117 g ( R )-HPBE L −1 d −1 , marking a substantial 74.5-fold increase over the batch system.
研究领域:
生物化学
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制药工程
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酶工程
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DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2022
·
IF 6.056
Renjie Zhou,Cheng Yang,Ting Xie,Jian Zhang,Chenqiang Wang,Ziqiang Ma,Lianfu Zhang
Amadori compounds (ACs) are the first stable product formed in the initial stage of the Maillard reaction and are widely present in processed foods. In addition to being widely known as the flavor precursors, ACs have attracted attention in recent years because many functions have been revealed, including ACE inhibition. In this work, we synthesized four ACs ( N -(1-Deoxy-D-fructos-1-yl)-glycine (Fru-Gly), N -(1-Deoxy-D-fructos-1-yl)-proline (Fru-Pro), N -(1-Deoxy-D-fructos-1-yl)-serine (Fru-Ser) and N -(1-Deoxy-D-fructos-1-yl)-threonine (Fru-Thr)) and characterized them using UPLC-Q-TOF-MS spectra. The four ACs’ angiotensin-converting enzyme (ACE) inhibitory activities were verified for the first time, the IC 50 value range was 1.447–4.204 mmol/L, the Fru-Gly has the best effect. And all the four ACs showed good digestion stability, the content remained above 70% after 5 h of continuous digestion simulation. In order to reveal the mechanism of ACs inhibiting ACE, we selected Fru-Gly, beacuse of the best inhibitory effect, to carried out enzyme kinetics, circular dichroism spectroscopy, fluorescence spectroscopy, thermodynamics and molecular docking analysis. The results showed that Fru-Gly was a noncompetitive inhibitor, it spontaneously binds to the inactive site of ACE by hydrogen bond and causes the conformation change of ACE to inactivate it. The reaction is easier to proceed at low temperatures.
研究领域:
食品科学
·
生物化学
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DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2017
·
IF 3.154
Long Liu,Yanan Wei,Qing Chang,Huaju Sun,Kungang Chai,Zuqiang Huang,Zhenxia Zhao,Zhongxing Zhao
A novel, moderately hydrophilic peptide (RYL) with high ACE-inhibitory activity was screened ultrafast via a concept of waste conversion using waste. This novel peptide was screened from silkworm pupa using an Fe-doped porous biocarbon (FL/Z-SE) derived from silkworm excrement. FL/Z-SE possessed magnetic properties and specific selection for peptides due to Fe’s dual functions. The selected RYL, which has moderate hydrophilicity (LogP = −0.22), exhibited a comparatively high ACE-inhibitory activity (IC50 = 3.31 ± 0.11 μM). The inhibitory kinetics and docking-simulation results show that, as a competitive ACE inhibitor, RYL formed five hydrogen bonds with the ACE residues in the S1 and S2 pockets. In this work, both the screening carbon material and the selected ACE-inhibitory peptide were derived from agricultural waste (silkworm excrement and pupa), which offers a new way of thinking about the development of advanced uses of the silkworm byproducts and wastes.
研究领域:
生物技术
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环境科学
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药学
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产品关联
DOI
PubMed
高影响因子
PROCESS BIOCHEMISTRY
·
2023
·
IF 4.4
Qi Liu,Shan Shao,Jingyu Bao,Syed Jalil Shah,Shumin Yue,Xinqi Luan,Qing Liu,Linguang Xing,Zhongfeng Shi,Zhenxia Zhao,Zhongxing Zhao
The 3D-QSAR model is one of the most effective techniques for predicting the peptide bioactivity based on their structure properties. The accuracy of model prediction is highly dependent on the bio-activity of the peptides in its dataset. However, the same peptide used for constructing the database was reported to have obviously different activities in different published articles, which may significantly decrease the prediction accuracy. Based on this, we chose ACE inhibitory (ACE-I) dipeptides that can be absorbed directly into the human small intestine as research object. Ten dipeptides were randomly selected from the literatures for molecular docking with ACE and synthesized to test their activity. Ultimately, a linear equation was developed between bio-activities and docking scores, which was used to revalidate the reliable ACE-I activities of all reported dipeptides for constructing a credible dataset of 3D-QSAR model. The prediction accuracy was significantly enhanced and the model we established had a high Q 2 = 0.840 and R 2 = 0.998. Furthermore, dipeptide CW was predicted and tested in vitro to achieve the highest ACE-I activity (IC 50 = 0.16 μM), and the inhibition mechanism was also investigated. This study introduced a new method for predicting bio-activity with high precision using the 3D-QSAR model.
研究领域:
生物信息学
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药物化学
·
生物化学
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产品关联
DOI
高影响因子
PROCESS BIOCHEMISTRY
·
2023
·
IF 4.4
Xiaoxuan Cai,Miaoen Huang,Xixiang Huang,Huan Liu,Tianji Wang,Li Li,Weiguang Yang,Hui Luo,Yingnian Lu
The organism Sipunculus nudus L is a good resource for marine peptides because of its rich protein. However, the disadvantage of biological peptides is their short metabolic half-life, therefore, structural modification of peptides is necessary. In this research, the enzymatic hydrolysis of Sipunculus nudus L was performed using trypsin and then peptides were isolated and purified from the hydrolysates, four novel tripeptides were identified by LC-MS-MS as Ser-Arg-Pro (SRP, m/z = 358.39), Pro-Arg-Pro (PRP, m/z = 368.43), Arg-Pro-Ala (RPA, m/z = 342.39), and Lue-Pro-Lys (LPK, m/z = 356.46), their ACE inhibitory activity was investigated with IC 50 value as following 0.046, 0.42, 0.97, and 6.54 mM, respectively. Because of its highest ACE inhibitory activity, SRP was further selected to modify the chemical structure for a sustained release agent DSPE-PEG-SRP. Compared with 7 h of free SRP, the release of DSPE-PEG-SRP in vitro was approximately 120 h. The ACE inhibition rate of SRP released from the synthetic product and the raw material SRP was almost the same, molecular docking demonstrated that ACE inhibitory activity was attributed to the formation of hydrogen bonds between SRP and ACE active sites . In conclusion, DSPE-PEG-SRP, a novel sustained release agent, has great potential and is expected to be further applied to treat hypertension.
研究领域:
海洋生物学
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药学
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生物化学
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产品关联
DOI
高影响因子
Foods
·
2022
·Vol. 11
·期 14
·2083
·
IF 5.561
Ye Dong,Wen Yan,Yi-Qi Zhang
The physicochemical, structural properties, antioxidant, and angiotensin I-converting enzyme (ACE) inhibitory activities of fish skin protein hydrolysate (SPH) that were freeze-dried (SPH-FD) and spray-dried (SPH-SD) were investigated. SPH-SD showed abundant volatile compounds, higher DPPH radical scavenging activity and ferrous iron chelating activity than SPH-FD, while the ABTS radical scavenging activity and ACE inhibitory activity were not influenced by the drying method. Amino acid compositions showed a higher proportion of proline and hydroxyproline residues in SPH-FD. The major molecular weights were both distributed below 1000 Da. SPH-SD had spherical structures, while SPH-FD had glass shard-like structures. The results indicated that the drying method could affect the physicochemical properties of hydrolysates, and SPH-SD showed potential prospects in developing functional fortified foods.Keywords:fish skin hydrolysate;freeze drying;spray drying;antioxidant;ACE inhibitory activity
研究领域:
食品科学
·
生物化学
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DOI
PubMed
高影响因子
Foods
·
2025
·Vol. 14
·期 7
·1209
·
IF 5.1
Kanzhen Liu,Cuiping Pang,Qinghua Li,Jianghua Li,Guocheng Du,Guoqiang Zhang
Bioactive components of abalone and other marine organisms have attracted significant attention owing to their functional performance. The development of peptides with bioactivity like angiotensin-converting enzyme inhibitory (ACEi) and antioxidant properties is of great significance for chronic disease management and drug discovery. In this study, according to the issues of low utilization rate and bioactive content from the hydrolysate of abalone, single-factor and orthogonal experiments were designed to improve the utilization rate of abalone protein, and step hydrolysis with specific proteases was used to improve the overall biological activity of the hydrolysate. A total of 1937 peptide sequences were obtained from the highly bioactive components after separation and peptidomic analysis. Through virtual screening and molecular docking, 14 peptides exhibiting ACEi activity were identified and synthesized for experimental verification, with IC 50 values ranging from 0.05 to 0.54 mg/mL. Notably, nine of these peptides were powerful antioxidants. The developed step enzymatic hydrolysis and in silico screening-assisted preparation also provided a feasible and efficient method for exploring more bioactive peptides from diverse biomasses.
研究领域:
海洋生物学
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药物化学
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食品科学
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产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Xianjiang Ye,Ran Yang,Zhizhi Yang,Baote Huang,Talha Riaz,Chao Zhao,Jicheng Chen
Food-derived peptides with angiotensin-I-converting enzyme inhibitory (ACEI) activity have garnered significant research attention. This study focused on examining ACEI peptides from Porphyra haitanensis and investigating their potential inhibitory mechanisms through a combination of in silico and in vitro methods. Five novel potential ACEI peptides (ASF: Ala-Ser-Phe, EIIL: Glu-Ile-Ile-Leu, LCVPR: Leu-Cys-Val-Pro-Arg, LDVVN: Leu-Asp-Val-Val-Asn, and LSPSW: Leu-Ser-Pro-Ser-Trp) were identified through a combination of peptidomics and in silico bioinformatics analysis, of which LSPSW showed the highest activity (IC 50 : 5.27 ± 0.53 μM). After simulated gastrointestinal digestion (SGD), the activities of LCVPR and LSPSW remained almost unchanged. The results from inhibition kinetics and molecular docking demonstrated that ACEI peptides with differing inhibition mechanisms could successfully bind to ACE through hydrogen bonds. Based on network pharmacology analysis and cellular experiments, LSPSW exerted antihypertensive effects by regulating the expression levels of peroxisome proliferator activated receptor gamma (PPARG), prostaglandin-endoperoxide synthase 2 (PTGS2), and renin (REN). This research provided a novel theoretical foundation for the high-value application of P. haitanensis .
研究领域:
食品科学
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生物信息学
·
药理学
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产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2017
·
IF 2.329
Honest N.E. Kessy,Kai Wang,Lei Zhao,Molin Zhou,Zhuoyan Hu
Enzyme-assisted extraction and biotransformation of phenolic compounds from litchi pericarps were investigated. Pectinase, β-glucosidase and tannase were used individually and sequentially. The effects of various enzymes and hydrolysis conditions on recovery and biotransformation of phenolic compounds were evaluated. The results indicated that the optimum conditions for enzymatic extraction were 3% (w/w) of pectinase, 1% (w/w) of tannase and 1% (w/w) of β-glucosidase, incubation time and temperature were 180 min and 35 °C, respectively. Under these conditions, pectinase treatment was more efficient in liberating phenolic compounds compared to tannase and β-glucosidase. Sequential enzyme treatment produced more phenolic compounds than the untreated litchi pericarps. As revealed by HPLC, apart from the phenolic compounds observed in pectin and pectin–tannase treated litchi pericarps, quercetin was observed in the pectinase-β-glucosidase and pectinase-β-glucosidase-tannase treated samples, indicating that flavonols probably underwent transformation from glycosylated (rutin) to deglycosylated (quercetin) during sequential enzymatic treatments. Ferric reducing antioxidant power, DPPH radical scavenging capacity, and angiotensin I-converting enzyme inhibition capacity of the enzyme produced extracts were significantly improved compared to the untreated sample. These results suggest that enzyme-assisted produced extracts could be a promising ingredient in developing natural functional foods, and in pharmaceutical products against hypertension in humans.
研究领域:
食品科学
·
植物化学
·
药理学
✓ 已验证
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2021
·
IF 4.952
Peng Zhang,Chang Chang,Haijie Liu,Qiaojuan Yan,Zhengqiang Jiang
Three protein-rich materials were pretreated with a self-designed alkaline electrolyzed water (AEW) pretreatment equipment to promote the release of angiotensin I-converting enzyme (ACE) inhibitory peptides by improving hydrolysis efficiency. According to degree of hydrolysis (DH) and ACE inhibitory activity, defatted walnut meal (DWM) was selected to study antihypertensive activity by hydrolysis in assistance of AEW pretreatment. It was found DWM pretreated with AEW at pH 11.5 followed by Alcalase hydrolysis exhibited the highest DH (21.96%), protein recovery rate (94.76%), and ACE inhibitory activity (IC 50 of 0.137 mg/mL), in which AEW pretreatment resulted in the transformation of α-helix and β-turn to random coil and the destruction of hydrogen bonds to expose more cleavage sites to enhance hydrolysis efficiency. Thereafter, ACE inhibitory peptides GIENYRVV (IC 50 of 0.102 mg/mL) and AGRISTVNSHTLPVL (IC 50 of 0.20 mg/mL) were identified from the hydrolysate. Overall, AEW pretreatment was an innovative strategy to promote the release of antihypertensive peptides during hydrolysis.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）