ADVANCED FUNCTIONAL MATERIALS
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2025
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IF 19
Shibo Liu,Hao Wang,Xiaohan Mei,Zehao Yu,Dapeng Zeng,Xiangjun Pan,Boda Ying,Xinyu Xu,Chuanyao Dong,Si Pu,Xiaoming Dong,Ruiyan Li,Yanguo Qin
Rapid rehabilitation of the ligament-bone interface following anterior cruciate ligament (ACL) reconstruction remains challenging due to poor vascularization and inadequate integration between graft, bone tunnel, and scaffold. Conventional rehabilitation stimuli are limited by their shallow tissue penetration, which fails to effectively target the bone tunnel. This study proposes a synergistic strategy using a dual calcium/copper reservoir-functionalized scaffold with trans-tissue mild photothermal therapy (mPTT, 40–42 °C) to promote phase-specific healing. A 3D-printed polyetherimide (PEI) scaffold incorporating Calcium carbonate (CaCO 3 ) serves as a calcium reservoir, while it is modified with a Cupric oxide-polydopamine (CuO-PDA) coating, serving both as a copper reservoir and a photothermal agent. Upon implantation, the rapid release of Cu 2+ accelerates early-stage vascularization, while sustained release of Ca 2+ creates a calcium-enriched osteogenic microenvironment, promoting osteogenic differentiation and bone regeneration. Crucially, near-infrared light-triggered activation of the CuO-PDA coating enables trans-tissue mPTT. This upregulates heat shock protein expression, which synergistically enhances reservoir-driven osteogenesis and collagen remodeling. This study marks the first application of mPTT in ligament-bone healing. This synergistic platform facilitates direct thermal stimulation and in situ repair at the ACL reconstruction site, offering a novel therapeutic strategy to enhance post-surgical recovery following ligament reconstruction with bone–interface fixation scaffolds.
研究领域:
骨科生物材料
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组织工程
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再生医学
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运动医学
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生物医学工程
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分子骨科学
✓ 已验证
产品关联
DOI
高影响因子
LANGMUIR
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2023
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IF 3.9
Fang Chen,Li Yin,Teng He,Tao Chen,Hangbo Yue,Chufen Yang
To enhance the stability of the polymeric micelles and optimize their drug-controlled release ability, three disulfide-linked polyethylene glycol methyl ether methacrylate–disulfide–poly(ε-caprolactone-co-γ-amine−ε-caprolactone) (PPEGMA-SS-P(CL-co-ACL)) polymers were synthesized and characterized by 1H NMR, GPC, and FT-IR successfully, and their dual pH/reduction-responsive cross-linked polymeric micelles were prepared for paclitaxel (PTX) delivery by using 2,3-dimethylmaleic anhydride (DMMA) as the cross-linking agent. The PTX loading capacity (LC) and encapsulation efficiency (EE) values of the cross-linked micelles formed by PPEGMA8-SS-P(CL47-co-ACL15) achieved were 23.96% and 71.58%, slightly higher than those of un-cross-linked micelles. Both particle sizes of blank micelles and in vitro drug release of PTX-loaded micelles confirmed that compared with those un-cross-linked micelles, the cross-linked micelles were more stable at pH 7.4 + 0 mM DTT, with a PTX cumulative release of 13% at 120 h, while the PTX cumulative release of the cross-linked micelles at pH 5.0 + 10 mM DTT were close to that of un-cross-linked micelles after 60 h, indicating the successful reversible cross-linking and smooth drug release of the cross-linked micelles. The cytotoxicity assay showed that PPEGMA8-SS-P(CL47-co-ACL15) and its cross-linked micelles had low cell cytotoxicity, and both PTX-loaded micelles revealed a certain inhibitory effect on HepG2 cells. These results revealed that the dual pH/reduction-responsive cross-linked polymeric micelles prepared from PPEGMA8-SS-P(CL47-co-DCL15) were a promising candidate for PTX delivery.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Science
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2025
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IF 14.1
Yaping Yue,Tingting Lu,Xiaoyuan Guo,Biao Liu,Shiteng Lv,Heba A. M. Abdalla,Xuelei Lai,Ruihui Zhang,Jianpin Guo,Yu Zhao,Dao-Xiu Zhou,Shaoli Zhou
SAGA (Spt-Ada-Gcn5 acetyltransferase) is a highly conserved histone acetyltransferase (HAT) complex in eukaryotes, playing a crucial role in regulating gene transcription during development. The complex consists of two core components: GCN5, the HAT subunit, and ADA2, which primarily functions as an adaptor and enhances the complex HAT activity. Beyond its well-established roles, it is discovered in this work that ADA2 in rice possesses a broadly existing intrinsically disordered region (IDR) that directs the formation of nuclear condensates. Moreover, ADA2 is found to interact with a subunit of ATP-citrate lyase (ACL), an enzyme that produces acetyl-CoA, leading to the formation of a GCN5-ADA2-ACL (GAA) complex. ADA2 promotes the condensation of both GCN5 and ACL in vivo. Within these condensates, ACL contributes to the production and enrichment of acetyl-CoA, thereby promoting histone acetylation. Genetic evidence showed that knock-out or suppression of these genes led to similarly diminished root meristem zone sizes and reduced branch primordia numbers, accompanied by significant reductions in genomic H3K9 acetylation and transcriptional attenuation of essential genes for meristem function. In summary, the findings unveil a novel mechanism of HAT action by forming phase separation to enrich acetyl-CoA within nuclear puncta, facilitating histone acetylation at target genes essential for meristem development.
研究领域:
植物生物学
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分子遗传学
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表观遗传学
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发育生物学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PROGRESS IN ORGANIC COATINGS
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2023
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IF 6.6
Junhua Chen,Qiuping Ke,Shiting Li,Xuan Wang,Zhihao Zeng,Can Liu,Ruli Mo,Pran Gopal Karmaker,Zhihui Xie,Qiwen Yong
In this study, a UV-curable anti-smudge cathodic electrophoretic coating was developed by free radical copolymerization of various acrylic monomers using heptadecafluorodecyl acrylate (FOEA) as a functional monomer to provide a low surface energy structure to the acrylate precursor. The precursor was then grafted with the synthetic double-bond terminated IPDI-HEA on the precursor, and finally dipentaerythritol penta/hexaacrylate (5-Acl) was introduced as a crosslinker for the subsequent cathodic electrophoresis and UV-curing process. The chemical structure and surface fluorine content of the fluorinated waterborne polyacrylate (FWPA) were confirmed by 1 H NMR, FT-IR, and XPS , respectively. The surface morphology and roughness of the coating were assessed using SEM and AFM instruments. The contact and sliding angles of water, diiodomethane, and hexadecane were determined. The self-cleaning capability was demonstrated by measuring the oil-based and aqueous epoxy paints, as well as carbon powder sliding on the coated tinplate surface. The anti-graffiti property was also integrated with a red marker. The resistance to strong acid, salt spray, impact, friction, and bending were characterized. All these results showed remarkable self-cleaning and anti-graffiti capabilities of the as-prepared UV-curable electrophoretic coating. Meanwhile, the coating displayed exceptional robustness in resisting chemical corrosion and mechanical damage. This newly developed UV-curable anti-smudge cathodic electrophoretic coating has great application potential in various metal protection industries.
研究领域:
材料科学
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涂层技术
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表面化学
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腐蚀工程
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Bioengineering and Biotechnology
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2023
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IF 5.7
Eunshinae Cho,Yi Qiao,Changan Chen,Junjie Xu,Jiangyu Cai,Yamin Li,Jinzhong Zhao
Features of black phosphorous (BP) nano sheets such as enhancing mineralization and reducing cytotoxicity in bone regeneration field have been reported. Thermo-responsive FHE hydrogel (mainly composed of oxidized hyaluronic acid (OHA), poly-ε-L-lysine (ε-EPL) and F127) also showed a desired outcome in skin regeneration due to its stability and antibacterial benefits. This study investigated the application of BP-FHE hydrogel in anterior cruciate ligament reconstruction (ACLR) both in in vitro and in vivo , and addressed its effects on tendon and bone healing. This BP-FHE hydrogel is expected to bring the benefits of both components (thermo-sensitivity, induced osteogenesis and easy delivery) to optimize the clinical application of ACLR and enhance the recovery. Our in vitro results confirmed the potential role of BP-FHE via significantly increased rBMSC attachment, proliferation and osteogenic differentiation with ARS and PCR analysis. Moreover, In vivo results indicated that BP-FHE hydrogels can successfully optimize the recovery of ACLR through enhancing osteogenesis and improving the integration of tendon and bone interface. Further results of Biomechanical testing and Micro-CT analysis [bone tunnel area (mm2) and bone volume/total volume (%)] demonstrated that BP can indeed accelerate bone ingrowth. Additionally, histological staining (H&E, Masson and Safranin O/fast green) and immunohistochemical analysis (COL I, COL III and BMP-2) strongly supported the ability of BP to promote tendon-bone healing after ACLR in murine animal models.
研究领域:
组织工程
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再生医学
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骨科手术
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生物材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）