JOURNAL OF BIOLOGICAL CHEMISTRY
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2025
·
IF 3.9
Bao-Di Ma,Jia-Yi Li,Jian-He Xu,Tao Yu,Xu-Dong Kong
Methanol dehydrogenase (MDH), a representative of Type III alcohol dehydrogenases (ADHs), plays a pivotal role in methanol assimilation pathways, making it a key enzyme for the biosynthesis of chemicals and fuels from one-carbon feedstocks. An activator protein belonging to the Nudix hydrolase family, ACT, was found to increase the activity of MDH by 40-fold. Despite the widespread observation of this in vitro activation phenomenon in pairs of type III alcohol dehydrogenases and Nudix hydrolases, the mechanistic details have remained unresolved for decades. Here, we uncover a regulation mechanism in which MDH activation arises from the hydrolytic removal of ADP-ribose (ADPR), a potent inhibitor derived from NAD + degradation, by the ADPRase activity of ACT. This discovery challenges the previously proposed 'activation' models, revealing that ACT-mediated ADPR clearance disinhibits MDH rather than directly enhancing catalysis. By combining crystallographic analysis, kinetics, and inhibition assays, we demonstrate that ADPR inhibits MDHs with submicromolar K i values, highlighting its potential regulatory role in metabolic networks. Our findings redefine the widespread ‘activation’ of type III ADHs, providing valuable insights into alcohol metabolism and new directions for engineering synthetic methanol utilization pathways.
研究领域:
生物化学
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结构生物学
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酶学
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代谢工程
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微生物学
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DOI
PubMed
高影响因子
Nature Nanotechnology
·
2025
·
IF 34.9
Huang Wei-Qiang,You Wei,Zhu Ya-Qi,Gao Fan,Wu Zhi-Zhi,Chen Guang,Xiao Jun,Shao Qi,Wang Long-Hai,Nie Xuan,Zhang Ze,Hong Chun-Yan,You Ye-Zi
Autophagosome cancer vaccines can promote cross-presentation of multiple tumour antigens and induce cross-reactive T cell responses. However, so far, there is no effective method for obtaining a highly immunogenic autophagosomal cancer vaccine because autophagosomes, once formed, quickly fuse with lysosomes and cannot easily escape from cells. Here we report a functional Ti 2 NX nanodot that caps the autophagosome membrane lipid phosphatidylinositol-4-phosphate, blocking the fusion of autophagosomes with lysosomes and producing stable nanodot-coated autophagosomes in tumours. The formed nanodot-coated autophagosomes can escape from cancer cells to lymph nodes, where they activate tumour-specific T cells. We show that our approach reduces tumour burden and provide long-term immune surveillance protection for cured mice. This work provides a method for the direct formation of personalized autophagosome-based cancer vaccines in vivo, offering a promising strategy for tumour treatment.
研究领域:
肿瘤学
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免疫学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
Sustainability
·
2022
·Vol. 14
·期 19
·12872
·
IF 3.889
Zihan Lang,Lidong Xue
Triclosan (TCS) has been widely employed as active ingredient in household products and has received attention for its hepatotoxicity, endocrine disruption and disturbance on immune function. Polylactic acid (PLA) has been highlighted as an alternative biodegradable microplastic, and the knowledge about the adsorption affinity towards TCS is limited. In this study, the ability to act as carrier of TCS by PLA and non-biodegradable microplastics, including polystyrene (PS), polyvinyl chloride (PVC) and polyethylene (PE) with different particle sizes were explored. The influence factors (e.g., dosage, pH and salinity), adsorption kinetics and isotherms were also investigated. Batch experiment results indicated that the TCS adsorption onto PLA and non-biodegradable microplastics exhibited a pronounced pH-dependent pattern (pH of 4, 7 and 10), and the adsorption capacity decreased gradually as pH increased. Furthermore, the adsorption capacity of TCS on PS, PVC and PE decreased as salinity increased from 0 to 3.5%, while no significant inhibition for the sorption capacity of PLA was observed. The adsorption kinetic data of TCS was best fitted with the pseudo-second order model. The Freundlich model with R2(0.999) was suitable to describe the adsorption isotherms of TCS on PLA, while the isotherms data of TCS on PS, PVC and PE was fitted by linear and Freundlich model. The higher adsorption capacity of PLA (38.6 mg g−1) compares to those of PS, PVC and PE (31.3, 11.4 and 9.64 mg g−1, respectively), illustrated by the fact that the physicochemical properties of microplastics have a noticeable impact on adsorption process, and the biodegradable PLA is a stronger vector than the non-biodegradable microplastics.Keywords:triclosan;polylactic acid;vector;pH-dependent
研究领域:
环境科学
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材料科学
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DOI
高影响因子
PLANT MOLECULAR BIOLOGY
·
2025
·Vol. 115
·期 4
·1-21
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IF 3.8
Xu Yao,Li En,Cao Weiping,Zhang Yingchao,Zhang Xinfang,Liu Zhaoyu,Cai Shanshan,Wang Jiayu,Cheng Fansheng,Chen Ruibing,Gao Ting
Scutellaria baicalensis , a traditional medicinal plant originating in China, is widely cultivated for its therapeutic properties. The main bioactive substances in S. baicalensis are flavonoids, which exhibit extensive antibacterial and antiviral activities. However, the contents of these valuable natural product ingredients are relatively low in the plant. MYB transcription factors play crucial roles in regulating plant secondary metabolism, including flavonoid biosynthesis. While the regulation of MYB transcription factors has been extensively studied in various species, research on their role in S. baicalensis remains relatively scarce. In this study, we identified SbMYB111, belonging to the S7 subgroup of R2R3-MYB transcription factors, which functions as a transcriptional activator and is localized in the nucleus. Through heterologous overexpression of SbMYB111 in Arabidopsis thaliana and suppression expression in S. baicalensis , we demonstrated that SbMYB111 acts as a positive regulator in the biosynthesis of flavonoids. Furthermore, the yeast one-hybrid and dual-luciferase reporter gene assays validated that SbMYB111 activates the expression of SbC4H2 , a key enzyme gene in the flavonoid biosynthesis pathway. This study provides a theoretical basis for understanding the transcriptional regulation mechanism of flavonoid synthesis and further developing medicinal resources of S. baicalensis .
研究领域:
植物学
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生物化学与分子生物学
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遗传学
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DOI
PubMed
高影响因子
Current Medical Science
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2024
·
IF 2
Yao Chen-guang,Zhao Zi-jia,Tan Ting,Yan Jiang-ning,Chen Zhong-wei,Xiong Jun-tao,Li Han-luo,Wei Yan-hong,Hu Kang-hong
Objective Lindqvist-type polyoxometalates (POMs) exhibit potential antitumor activities. This study aimed to examine the effects of Lindqvist-type POMs against breast cancer and the underlying mechanism. Methods Using different cancer cell lines, the present study evaluated the antitumor activities of POM analogues that were modified at the body skeleton based on molybdenum-vanadium-centered negative oxygen ion polycondensations with different side strains. Cell colony formation assay, autophagy detection, mitochondrial observation, qRT-PCR, Western blotting, and animal model were used to evaluate the antitumor activities of POMs against breast cancer cells and the related mechanism. Results MO-4, a Lindqvist-type POM linking a proline at its side strain, was selected for subsequent experiments due to its low half maximal inhibitory concentration in the inhibition of proliferation of breast cancer cells. It was found that MO-4 induced the apoptosis of multiple types of breast cancer cells. Mechanistically, MO-4 activated intracellular mitophagy by elevating mitochondrial reactive oxygen species (ROS) levels and resulting in apoptosis. In vivo , breast tumor growth and distant metastasis were significantly reduced following MO-4 treatment. Conclusion Collectively, the results of the present study demonstrated that the novel Lindqvist-type POM MO-4 may exhibit potential in the treatment of breast cancer.
研究领域:
肿瘤学
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药理学
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分子生物学
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细胞生物学
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DOI
PubMed
高影响因子
RSC Advances
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2017
·Vol. 7
·期 87
·55459-55470
·
IF 3.108
Caixia Liu,Qiuxai Shen,Wenwen Zheng,Yao Lv,Xinyu Chen,Xiaoheng Li,Qiqi Zhu,Xiaoling Guo,Renshan Ge,Chao Li
In order to evoke an efficient immune response to prevent and eliminate infectious and malignant diseases, it is critical to develop more effective and safer vaccines or adjuvants to enhance the antigenicity of natural antigens. The objective of this study is to produce nanoparticles composed of poly(methyl vinyl ether-co-maleic anhydride) (PVM/MA) and the model antigen ovalbumin (OVA), termed as p-OVAs, to assess the antigen sustained release property and the effectiveness in the immune responses in BALB/c mice. The results showed that the p-OVAs were spherical, evenly distributed with an average diameter of ∼290 nm, and had a sustained release property in vitro. After immunization of BALB/C mice with p-OVA, strong OVA-specific cellular and humoral immune responses were observed in vivo. The whole blood cell tests and histological results also suggested that immunization with p-OVA is both effective and relatively safe. Overall, here we provide new insight into future vaccine and/or adjuvant design with PVM/MA, which is promising for the immunotherapy of cancer and autoimmune diseases.
研究领域:
药学
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免疫学
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纳米技术
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生物医学工程
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产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
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2014
·
IF 3.916
Qingyuan Niu,Kezheng Gao,Wenhui Wu
A cellulose nanofibril film is modified by chemical assembly of boronate-terminated conjugated polymer chains at its specific sites, C-6 carboxyl groups. The modified cellulose nanofibril film is used as a fluorescent sensor for nitroaromatic vapor. Thanks to the specific reactive sites, numerous loose cavities or pathways located in the film sensor's out-layer have been formed, and the fraction of easily accessible cavities of the novel fluorescent film sensor is up to 0.97, which could benefit the penetration and diffusion of analyte vapor. Therefore, the novel fluorescent film sensor exhibits high sensitivity toward nitroaromatic vapor with a fast response. The fluorescence quenching efficiency of the chemical-assembly film sensor is about 3 times larger than that of the spin-cast film sensor using the same conjugated polymer for 600 s exposure to DNT vapor. In addition, the novel fluorescent film sensor shows good reversibility.
研究领域:
材料科学
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化学传感
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产品关联
DOI
PubMed
高影响因子
Journal of Cleaner Production
·
2021
·
IF 9.297
Maocai Shen,Zhuotong Zeng,Long Li,Biao Song,Chengyun Zhou,Guangming Zeng,Yaxin Zhang,Rong Xiao
As an important contributor of environmental microplastics , wastewater treatment plants have attracted extensive attention. The negative effect of suspended particulate matter on disinfection effect has also been established. The main aims of this study were to understand the potential effects of granular polyethylene microplastic and fibrous polyamide microplastics on ultraviolet and chlorine disinfection in water/wastewater treatment. In the laboratory, microplastics were exposed to different disinfection methods, which represented the disinfection dose and disinfection conditions of real water and wastewater treatment. Escherichia coli (8099) were selected as the experimental object. Microplastic suspended solids were closely related to water turbidity and UV transmittance at 254 nm. The occurrence of microplastics significantly reduced the efficiency of ultraviolet and chlorine disinfection in water/wastewater treatment, and the efficiency decreased with the increase of microplastics concentration or the decrease of disinfectant dosage. When the concentration of microplastics reached a certain level (>50 mg L −1 ), even if the exposure dose continued to increase, the disinfection efficiency could not be improved. Microplastics can reduce the concentration of disinfectants around them by reacting with disinfectants, thus protecting the microorganisms enriched on the surface of microplastics. Although the harsh conditions tested here are rarely met in a typical water treatment process, it represented a worse result. These results were intended to be used by water treatment plants to predict the effects of microplastics in the secondary effluent on UV and chlorine disinfection processes .
研究领域:
环境科学
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微生物学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
·
IF 8.2
Hao Xu,Jiaxu Li,David Julian McClements,Hao Cheng,Jie Long,Xinwen Peng,Zhenlin Xu,Man Meng,Yidong Zou,Guanxiong Chen,Zhengyu Jin,Long Chen
Starch has great potential to replace petroleum-based plastics in food packaging applications. However, starch films often exhibit poor mechanical and barrier properties, and are vulnerable to moisture and bacterial contamination . This study proved that the incorporation of eggshell powder (ES) enhanced the hydrogen bonding in starch-based films significantly, which contributed to improved tensile strength , Young's modulus , and water resistance of the films. The performance of ES-incorporated films could be optimized by adjusting the size, concentration, and surface property of ES in the film matrix. Notably, adsorbing epigallocatechin gallate (EGCG) on the surface of porous ES contributed to enhanced dispersibility of the fillers in the film matrix, which increased the tortuous path of light, water vapor, and oxygen have to take through the films, resulting in increased UV screening performance, water vapor and oxygen barrier property of the films by 60 %, 7.2 %, and 27.9 %, respectively. Meanwhile, loading EGCG in ES also enable superior antibacterial activity of the final films. This study suggests that eggshell fillers offer a sustainable means of improving the functional performance of starch-based films, which may increase their application as packaging materials in the food industry.
研究领域:
食品科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
iScience
·
2025
·Vol. 28
·期 12
·
IF 4.1
Dan Chen,Qingqing Ma,Xin Li,Tao Yang,Shang Wu,Yanju Wang,Deyu Hu
Intercellular movement plays an important role in plant virus infection and transmission, which provides an opportunity for antiviral agents that interfere with the intercellular motion. However, there are still few compounds that directly interfere with the movement of viruses between cells. Herein, a series of aurone derivatives containing sulfonamide active groups were designed and synthesized. Among them, compound D13 effectively inhibits virus infection by forming a stable hydrogen bond with Pro154 (P154) of potato virus Y (PVY) coat protein (CP). Further studies have shown that D13 can effectively interfere with the process of P154-mediated viral intercellular trafficking in plasmodesmata (PD). This study emphasizes the core position of virus intercellular movement in plant virus diseases and its inhibition strategy and confirms that the aurone derivative D13 is a candidate molecule for antiviral pesticides or intercellular movement inhibitors, providing a new direction for plant virus prevention and control.
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Zhenfei Yang,Xinnian Xia,Ming Fang,Longlu Wang,Yutang Liu
The selective oxidation of 5-hydroxymethylfurfural (HMF) to 2,5-dicarbofuran (DFF), and 2,5-furanedicarboxylic acid (FDCA) is essential to its practical application. Herein, a novel Pt/ZIS/MnO 2 (PMZIS) photocatalysts with photothermal effect were well designed for selective and controlled oxidation of HMF toward DFF or FDCA. The product distribution of HMF photo-oxidation on PMZIS can be regulated by the photothermal effect. At a low temperature (10 °C) with PMZIS, DFF is the primary oxidation product with the yield of 54.6% and selectivity of 85.4%. At a high temperature (60–70 °C) induced by photothermal effect, the FDCA can be obtained with the yield of 61.5 % and selectivity of 82.9%. Moreover, the Z-scheme transfer pathway and Pt cocatalysts realize the spatial charge separation structure of PMZIS heterojunctions. This unique structure greatly inhibits charge recombination and maximizes the activation ability of molecular oxygen to efficiently generate of the reactive oxygen species (e.g., 1O 2 and O 2– ), thus significantly improving the photocatalytic performance. This work would provide valuable insight in the product regulation through photothermal effect for the photocatalytic specific selective oxidation of organic molecules.
研究领域:
化学
·
材料科学
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产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2023
·
IF 9.5
Chenguang Ding,Bo Wang,Jin Zheng,Mingzhen Zhang,Yang Li,Hsin-Hui Shen,Yingcong Guo,Bingxuan Zheng,Puxun Tian,Xiaoming Ding,Wujun Xue
Ischemia/reperfusion (I/R) injury causes excessive oxidative events and initiates destructive inflammatory responses, and it is an important promoter to the pathology of various pathema states. Ferroptosis is an iron-dependent type of nonapoptotic cell death accompanied by the accumulation of membrane lipid peroxide and consumption of polyunsaturated fatty acid, and it plays a key role in I/R injury diseases. Moreover, the excessive production of inflammatory cytokines contributes to the development of acute kidney injury. Here, we reported neutrophil membrane-coated copper-based nanoparticles (N–Cu5.4O@DFO NPs) for I/R kidney injury treatment. The highly biocompatible and stable N–Cu5.4O@DFO NPs showed excellent antioxidant and iron ion scavenging abilities in vitro. Our finding showed that the N–Cu5.4O@DFO NPs strategy could significantly accumulate in the inflammatory kidney, reduce oxidative damage events and inflammatory response, and finally achieve synergistic therapy against renal I/R injury. This work promotes the development of nanoantioxidant agents with multiple antioxidant properties for the therapy of other I/R injury diseases.
研究领域:
纳米医学
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药理学
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肾脏生理学
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炎症研究
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氧化应激
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）