查找使用阿拉丁产品的科研文献

[18F]AlF-NOTA-ADH-1: A new PET molecular radiotracer for imaging of N-cadherin-positive tumors

Liu Zhenfeng,Wen Guanghua,Huang Yuqiao,Dong Yanzhao,Wang Zewei,Alhaskawi Ahmad,Zhang Shuyi,Wang GuoLin,Ye Qianni,Zhou Haiying,Lu Hui,Dong Mengjie

Background: The cell adhesion molecule (CAM) N-cadherin has become an important target for tumor therapy. The N-cadherin antagonist, ADH-1, exerts significant antitumor activity against N-cadherin-expressing cancers.Methods: In this study, [18F]AlF-NOTA-ADH-1 was radiosynthesized. An in vitro cell binding test was performed, and the biodistribution and micro-PET imaging of the probe targeting N-cadherin were also studied in vivo.Results: Radiolabeling of ADH-1 with [18F]AlF achieved a yield of up to 30% (not decay-corrected) with a radiochemical purity of >97%. The cell uptake study showed that Cy3-ADH-1 binds to SW480 cells but weakly binds to BXPC3 cells in the same concentration range. The biodistribution results demonstrated that [18F]AlF-NOTA-ADH-1 had a good tumor/muscle ratio (8.70±2.68) in patient-derived xenograft (PDX) tumor xenografts but a lower tumor/muscle ratio (1.91±0.69) in SW480 tumor xenografts and lowest tumor/muscle ratio (0.96±0.32) in BXPC3 tumor xenografts at 1 h post-injection (p.i.) These findings were in accordance with the immunohistochemistry results. The micro PET imaging results revealed good [18F]AlF-NOTA-ADH-1 tumor uptake in pancreatic cancer PDX xenografts with strong positive N-calcium expression, while lower tumor uptake in SW480 xenografts with positive expression of N-cadherin, and significantly lower tumor uptake in BXPC3 xenografts with low expression of N-cadherin, which was consistent with the biodistribution and immunohistochemistry results. The N-cadherin-specific binding of [18F]AlF-NOTA-ADH-1 was further verified by a blocking experiment involving coinjection of a non radiolabeled ADH-1 peptide, resulting in a significant reduction in tumor uptake in PDX xenografts and SW480 tumor.Conclusion: [18F]AlF-NOTA-ADH-1 was successfully radiosynthesized, and Cy3-ADH-1 showed favorable N-cadherin-specific targeting ability by in vitro data. The biodistribution and microPET imaging of the probe further showed that [18F]AlF-NOTA-ADH-1 could discern different expressions of N-cadherin in tumors. Collectively, the findings demonstrated the potential of [18F]AlF-NOTA-ADH-1 as a PET imaging probe for non-invasive evaluation of the N-cadherin expression in tumors.

Identification of the ADH gene family in Trichosporon asahii and the role of TaADH_like in pathogenicity and fluconazole resistance

Liu Zhen,Ma Xiaoping,Zeng Xiangwen,Li Zhiguo,Liu Ruiguo,Luo Rongyan,Wang Weichen,Tahir Muhammad Salman,Wang Chengdong,Gu Yu

Alcohol dehydrogenase has been studied in regulation of fungal growth and development, stress response and pathogenesis, but its function in T. asahii remains unexplored. In this study, we analyzed the ADH gene family in T. asahii for the first time, identifying six ADH genes and containing conserved ADH_N and ADH_Zinc_N domains. We constructed an overexpression strain of the most significantly differentially expressed gene TaADH_like and compared its phenotypes with those of the wild-type strain, focusing on colony morphology, biofilm biomass, stress response, drug resistance, and pathogenicity. The results showed that TaADH_like overexpression reduced sensitivity to hypoxic conditions, altered the hyphae-to-yeast transition, and led to slower growth, decreased colonization ability, reduced tissue damage, and lower lethality. Increased osmotic stress sensitivity and the involvement of the HOG MAPK pathway in the hyphae-to-yeast conversion contributed to the reduced colonization capacity of T. asahii . Furthermore, the overexpression of TaADH_like promoted biofilm formation and led to a slight enhancement in fluconazole resistance in T. asahii . This study is the first to elucidate the function of the alcohol dehydrogenase gene in T. asahii , providing a foundation for future genetic research on this pathogen.

Improvement of chemosensitivity and inhibition of migration via targeting tumor epithelial-to-mesenchymal transition cells by ADH-1-modified liposomes

Zhaoming Guo,Wenqing Li,Yue Yuan,Kun Zheng,Yu Tang,Kun Ma,Changhao Cui,Li Wang,Bing He,Qiang Zhang

How to overcome drug resistance and prevent tumor metastasis is key to the success of malignant tumor therapy. In this paper, ADH-1 peptide-modified liposomes (A-LP) have been successfully constructed for restoring chemosensitivity and suppressing cancer cell migration. With a particle size of about 90 nm, this functionalized nanocarrier was loaded with fluorescent probe or paclitaxel (PTX). Cellular uptake studies showed that A-LP facilitated the delivery of anticancer drug to tumor cells undergoing EMT. Interestingly, this nanocarrier enhanced chemosensitivity by assessing the cell activity using CCK-8 assay. Further, the results of Wound scratch assay and Transwell migration assay showed the inhibition effect of this nanocarrier on tumor cell migration. Moreover, this nanocarrier exhibited significant tumor-targeting ability and anti-tumor efficacy in vivo. Collectively, A-LP might be a novel targeted drug delivery system to enhance the efficacy of chemotherapy and prevent tumor metastasis.

Novel pH-Responsive CaO2@ZIF-67-HA-ADH Coating That Efficiently Enhances the Antimicrobial, Osteogenic, and Angiogenic Properties of Titanium Implants

Xinlin Li,Mengfei Xu,Zhaoli Geng,Xinyi Xu,Xu Han,Lin Chen,Ping Ji,Yi Liu

Titanium-based implants often lead to premature implant failure due to the lack of antimicrobial, osteogenic, and angiogenic properties. To this end, a new strategy was developed to fabricate CaO2@ZIF-67-HA-ADH coating on titanium surfaces by combining calcium peroxide (CaO2) nanoparticles, zeolite imidazolate framework-67 (ZIF-67), and the chemical coupling hyaluronic acid–adipic acid dihydrazide (HA-ADH). We characterized CaO2@ZIF-67-HA-ADH with scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectroscopy, X-ray photoelectron spectroscopy (XPS), and inductively coupled plasma–atomic emission spectrometry (ICP–AES). The results demonstrated that CaO2@ZIF-67-HA-ADH was pH-sensitive and decomposed rapidly under acidic conditions, and it released inclusions slowly under neutral conditions. Antibacterial experiments showed that the CaO2@ZIF-67-HA-ADH coating had excellent antibacterial properties and effectively killed methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa (PAO-1). Cell experiments revealed that the CaO2@ZIF-67-HA-ADH coating promoted pro-osteoblast adhesion, proliferation, and differentiation and also promoted the migration and angiogenesis of human umbilical vein endothelial cells (HUVECs), exhibiting excellent osteogenic and angiogenic properties. In in vivo animal implantation experiments, the CaO2@ZIF-67-HA-ADH coating exhibited strong antimicrobial activity early after implantation and excellent osseointegration later after implantation. In conclusion, the pH-responsive CaO2@ZIF-67-HA-ADH coating conferred excellent antibacterial, osteogenic, and angiogenic properties to titanium implants, which effectively enhanced osseointegration of the implants and prevented bacterial infection; the coating shows promise for use in the treatment of bone defects.

Ganshuang granule plays a pharmacological role in anti-alcoholic and anti-hangover via regulating alcohol metabolism and affecting neurotransmitters

Qiannan Li,Yinghui Lu,Jinfeng Shang,Qi Song,Jiakang Jiao,Lei Bi,Tingyue Jiang,Xin Liu

Background To explore the effect of Ganshuang granule on anti-alcoholic and anti-hangover and its potential mechanism.Methods SPF SD rats’ drunken model and SPF Kunming mice’s hangover model were used as models.Results Ganshuang granule could significantly reduce sleep time, the time to climb in mice, and significantly prolong the tolerance time and shorten sleep time in rats (p < 0.05). The blood ethanol concentration of rats in each administration group was lower than that in the model group at each time point (p < 0.05). Compared with the control group, the activities of ADH and ALDH in the liver of the model group were significantly decreased (p < 0.05); the content of DA and 5-HT in the striatum of the model group was significantly increased (p < 0.05); and the activity of AchE in the hippocampus was significantly decreased (p < 0.05). The above processes could be improved and regulated in the drug administration group. Compared with the control group, there was no significant difference between ADH and ALDH in the serum of the model group (p > 0.05). However, the activities of ADH and ALDH in the liver of drunk rats could be upregulated by Ganshuang granule (p < 0.05).Conclusion Ganshuang granule has the pharmacological effects of anti-alcoholic and anti-hangover, which is related to regulating the activities of ADH and ALDH in the liver, the contents of DA and 5-HT in striatum, and the activity of AchE in the hippocampus.

Preparation and properties of Pue-loaded HA-ADH-PS nanomicelles

Huiru Wang,Yuanyuan Li,Yunpeng Min,Hang Zhang,Linkun Hao,Ru Zhang,Yunying Jiang,Yimin Song

Preparation, identification, and functional properties of ADH-activating and hepatoprotective potential egg white peptides

Yang Bingxin,Ma Lulu,Pan Li,Zeng Qian,Chang Xinping,Liu Xin,Dong Shijian,Wu Jijun,Li Shugang

Alcoholic liver disease (ALD) is a global health concern caused by chronic excessive alcohol consumption, and dietary intervention is a key strategy for improving ALD. This study aimed to develop egg white peptides (EWP) with enhanced alcohol metabolism functionality. By optimizing the stepwise enzymatic hydrolysis process, we successfully achieved activation rates of alcohol dehydrogenase (ADH) and ABTS free radical scavenging capacity in EWP of 35.76% and 43.12%, respectively. EWP showed adequate stability during processing and in vitro digestion. LC-MS/MS analysis identified 8,220 peptides. Computer-aided analysis screened four novel ADH-activating peptides (LAPGW, WLF, WPPP, and WFLPR). Molecular docking analysis confirmed that these peptides exhibit strong and stable binding to ADH through hydrophobic interactions, hydrogen bonds, and van der Waals forces. In an alcohol-damaged HepG2 cell model, these peptides significantly reduced levels of cellular damage markers (transaminases) by more than 29%. Mechanistic studies indicated these peptides exerted protective effects against alcoholic hepatocyte injury by accelerating alcohol metabolism and potentially modulating antioxidant enzyme activities—such as glutathione synthesis and superoxide dismutase—to synergistically alleviate oxidative stress. Graphical

An Industrial Scale Synthesis of Adipicdihydrazide (ADH)/Polyacrylate Hybrid with Excellent Formaldehyde Degradation Performance

Rui Zhu,Renjie Chen,Yunxia Duo,Saigang Zhang,Delong Xie,Yi Mei

A simple and versatile route for industrial scale synthesis of adipicdihydrazide (ADH)/polymer hybrids with excellent performance of formaldehyde degradation is proposed in this paper. The ADH compound is uniformly dispersed in poly(methyl methacrylate-butyl acrylate-methacrylic acid) (P(MMA-BA-MAA)) latex, which is validated by UV and dispersibility tests. The results illustrate that ADH has excellent compatibility and dispersion stability without affecting the film formation of the polymer latex. Furthermore, scanning electron microscope (SEM) and mapping analysis of the hybrid films also demonstrate that ADH is homogenously dispersed in the polymer matrix. Compared with neat polymers, the thermal properties of hybrid films are improved, for example,T0.5increases by 8.3 °C. According to qualitative tests of the 4-amino-3-hydrazino-5-mercapto-1,2,4-triazol-red/green/blue (AHMT-RGB) method, the hybrid films demonstrate high formaldehyde removal efficiency. On the basis of the semi-quantitative test of Fourier Transform infrared spectroscopy (FTIR) measurements, the rate of formaldehyde degradation can reach 1.034 × 102mol/(h·m3) for the hybrid film with 5 wt% ADH.Keywords:adipicdihydrazide;formaldehyde degradation;polyacrylate latex;hybrid film

Comparison of different ginsenosides with C-3 or C-6 sugar moieties on activities in alcohol-induced liver injury mice

Weimin Wang,Kaixuan Zhou,Zengshuai Fu,Yucheng Huang,Jianjun Deng,Daidi Fan,Haixia Yang

Background Alcohol-induced liver injury contributes to various liver diseases, with dietary interventions of ginsenosides being a promising solution. Ginsenosides, the major active compounds of Panax ginseng Meyer , are divided into protopanaxadiol (PPD) and protopanaxatriol (PPT) based on C-3 or C-6 glycosylation, which have anti-inflammatory and antioxidant effects, but structure-activity relationships remain unclear. Methods Using C57BL/C mice with alcohol-induced liver injury, we evaluated Rg5 and F4 effects on liver function, inflammation, lipid deposition, apoptosis, alcohol metabolism, and lipid synthesis. Results Rg5 (60 mg/kg) demonstrated significantly superior efficacy to F4 in alleviating alcohol-induced liver injury, reducing lipid deposition through 33.9% and 25.8% decreases in serum triglyceride (TG) and total cholesterol (TC) levels versus the F4 group. Additionally, Rg5 attenuated hepatic apoptosis by reducing BAX and cleaved-CASPASE-3 protein expression by 26.3% and 28.4% compared to F4. Rg5 enhanced alcohol metabolism through activation of alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH), thereby restoring AMP-activated protein kinase (AMPK) phosphorylation by 26.1% while reducing sterol regulatory element-binding protein 1 (SREBP-1) expression by 27.8% (compared to F4), with efficacy approaching that of positive control silymarin. Molecular docking revealed that the C-6 sugar moiety of F4 induced hydrogen bond donor repulsion, increasing hydrogen bond length and weakening binding stability with ADH, ALDH and AMPK, providing a structural basis for Rg5's superior hepatoprotective activity. Conclusion These findings highlight the critical influence of glycosylation position on hepatoprotective activity of ginsenosides, and provide insights into the structural modification of ginsenosides, which could contribute to the treatment of alcohol-related liver disease.

Development of compatible photocatalyst-enzyme hybrid system via Intensifying enzyme tolerance for enhanced chiral alcohol synthesis

Chun-Xian Cai,Zi-Wen Zhou,Chun-Xiu Liu,Yun-Jie Wei,Xiao-Qi Yu,Li-Hong Zhou,Na Wang

Photo-enzyme catalysis featuring sustainability and selectivity is attractive, but remains challenges from efficiency and enzyme inactivation. This work provides a compatible and efficient photocatalyst-enzyme hybrid system for chiral alcohol synthesis. A covalent organic framework (COF) possessing broad visible light absorption edge to 554 nm was used for photocatalytic NADPH regeneration, achieving 82 % yield in 90 min. Then the influence of photocatalysis on coupled enzyme Lb ADH was unveiled by fluorescence spectra and circular dichroism. Further a facile and mild strategy of entrapping Lb ADH into Ca 2+ /Mn 2+ doped alginate gel was carried out. Impressively, enzyme immobilized strategy not only enhances 3.2 times activity, but also protects Lb ADH from diverse harsh environments, thereby improving photo-enzyme catalytic yield from 26 % to 82 %. To our delight, this one-pot photo-enzyme catalytic system achieves highly yield (up to 99 %) with excellent enantioselectivity (90–99 ee %) for various chiral alcohols, and fulfills semipreparative scale preparation of ( R )-1-[3,5-Bis(trifluoromethyl)phenyl] ethanol, prochiral intermediate of Aprepitant. This work sheds light on the new synthetic route of chiral compounds and development of photo-enzyme catalytic field.

Preparation of ZIF@ADH/NAD-MSN/LDH Core Shell Nanocomposites for the Enhancement of Coenzyme Catalyzed Double Enzyme Cascade

Le Wang,Pengxue Sun,Yiyu Yang,Hanzhen Qiao,Hailong Tian,Dapeng Wu,Shuoye Yang,Qipeng Yuan,Jinshui Wang

The field of enzyme cascades in limited microscale or nanoscale environments has undergone a quick growth and attracted increasing interests in the field of rapid development of systems chemistry. In this study, alcohol dehydrogenase (ADH), lactate dehydrogenase (LDH), and mesoporous silica nanoparticles (MSN) immobilized nicotinamide adenine dinucleotide (NAD+) were successfully immobilized on the zeolitic imidazolate frameworks (ZIFs). This immobilized product was named ZIF@ADH/NAD-MSN/LDH, and the effect of the multi-enzyme cascade was studied by measuring the catalytic synthesis of lactic acid. The loading efficiency of the enzyme in the in-situ co-immobilization method reached 92.65%. The synthesis rate of lactic acid was increased to 70.10%, which was about 2.82 times that of the free enzyme under the optimal conditions (40 °C, pH = 8). Additionally, ZIF@ADH/NAD-MSN/LDH had experimental stability (71.67% relative activity after four experiments) and storage stability (93.45% relative activity after three weeks of storage at 4 °C; 76.89% relative activity after incubation in acetonitrile-aqueous solution for 1 h; 27.42% relative activity after incubation in 15% N, N-Dimethylformamide (DMF) solution for 1 h). In summary, in this paper, the cyclic regeneration of coenzymes was achieved, and the reaction efficiency of the multi-enzyme biocatalytic cascade was improved due to the reduction of substrate diffusion.Keywords:confinement effect;multi-enzyme catalytic cascade;in-situ synthesis;coenzyme recycling;co-immobilization enzyme

Determination of ADH in textiles using the HPLC-MS/MS method and the study of its adsorption behaviour towards formaldehyde

Jinxiong Tao,Ziwei Lin,Haixuan Zhang,Zhuoming Wu,Haihui Cao

In the textile industry, formaldehyde-based resins are used as finishers to make the fabrics crease-resistant, which are the main source of formaldehyde in textiles. In our practical study, there are cases that prove that textile products containing adipic dihydrazide (ADH) will continuously adsorb formaldehyde from the surrounding environment during storage. In this study, a high performance liquid chromatography-tandem mass spectrometry method was established for the precise determination of ADH in textiles. The method was optimized in terms of instrument conditions, extraction temperature, extraction time, and extraction mode. Under optimum test conditions, ADH was determined precisely with the linearity range of 0.05–2 mg L−1 and correlation coefficient (R2) of 0.9993. Recovery rate and repeatability were tested; the data showed that the recovery rate of ADH in textiles was in the range of 85–100%, and the RSD (relative standard deviation) was less than 10%. The ADH-positive textile samples were placed in designed environments for some time to adsorb formaldehyde. The adsorbed amounts of formaldehyde in the textile samples first increase and then decrease with time. The maximum amount of formaldehyde a sample can adsorb increases with an increase in its ADH content and will stop increasing once its ADH content exceeds 1700 mg kg−1. The placement environment has a little effect on the maximum adsorption capacity of the samples towards formaldehyde, but can significantly affect the adsorption rate and equilibrium adsorption capacity.