SEPARATION SCIENCE AND TECHNOLOGY
·
2019
·
IF 1.354
Mei Wang,Na Rao,Yuqing Liu,Jiale Li,Qunpeng Cheng,Jianfen Li
TETA or TETA-AEP mixtures were used as an activator to enhance CO2 capture in the MDEA solution. The effect of amount and type of activators, and the viscosity of absorbents on CO2 capture were discussed. The results showed that the positive effect of TETA-AEP mixtures on CO2 capture in the MDEA solution was greater than that of TETA. The optimal absorbent was No. V, whose CO2 absorption capacity/desorption efficiency was 3.08 times/1.18 times of No. І. The viscosity had a little influence on CO2 absorption and an obvious effect on CO2 desorption.
研究领域:
化学工程
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL IMMUNOPHARMACOLOGY
·
2025
·
IF 4.7
Zhaokun shi,Qinyu Peng,Shangqi Sun,Min Xiong,Xue Zhu,Huijuan Wang,Shiping Liu,Zhenwen Li,Lu Wang,Guang Li,Liying Zhan
Septic encephalopathy (SE), a neurocritical complication with alarmingly high mortality rates, represents a major diagnostic and therapeutic challenge in intensive care units (ICUs). Current diagnostic protocols rely predominantly on nonspecific clinical symptom assessment, while conventional laboratory biomarkers exhibit insufficient sensitivity for early detection. This diagnostic ambiguity contributes to delayed interventions and unfavorable patient prognoses. Mechanistically, prior studies in Alzheimer's disease (AD) have established that genetic ablation of C/EBP β or AEP (asparagine endopeptidase) mitigates neuroinflammation and disease progression. Intriguingly, our investigation reveals a pathophysiological paradox in SE: Conditional AEP knockout significantly attenuated sepsis-induced hippocampal damage, whereas C/EBP β knockdown unexpectedly exacerbated neuronal injury — findings directly opposing AD-related mechanisms. Notably, this study identifies mitochondrial dysfunction in hippocampal neurons as a previously unrecognized hallmark of SE, characterized by cristae fragmentation and mitochondrial membrane potential collapse. Multimodal analyses further demonstrate that AEP inhibition rescues synaptic plasticity through dual modulation of STat3 signaling and oxidative stress cascades. Importantly, our paradoxical findings regarding C/EBP β suggest context-dependent regulation of neuroinflammation, challenging the prevailing assumption of pathway conservation across neuropathologies. These translational insights not only elucidate the C/EBP β/AEP axis as a novel therapeutic target in SE but also warrant caution against extrapolating AD-derived mechanisms to sepsis-related encephalopathies.
研究领域:
神经科学
·
重症监护医学
·
分子生物学
·
免疫学
·
病理生理学
·
线粒体生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2021
·Vol. 19
·期 1
·1-18
·
IF 10.435
Wang Shan-Shan,Liu Zi-Kai,Liu Jing-Jing,Cheng Qing,Wang Yan-Xia,Liu Yan,Ni Wen-Wen,Chen Hong-Zhuan,Song Mingke
Background Discovery of early-stage biomarkers is a long-sought goal of Alzheimer’s disease (AD) diagnosis. Age is the greatest risk factor for most AD and accumulating evidence suggests that age-dependent elevation of asparaginyl endopeptidase (AEP) in the brain may represent a new biological marker for predicting AD. However, this speculation remains to be explored with an appropriate assay method because mammalian AEP exists in many organs and the level of AEP in body fluid isn’t proportional to its concentration in brain parenchyma. To this end, we here modified gold nanoparticle (AuNPs) into an AEP-responsive imaging probe and choose transgenic APPswe/PS1dE9 (APP/PS1) mice as an animal model of AD. Our aim is to determine whether imaging of brain AEP can be used to predict AD pathology.Results This AEP-responsive imaging probe AuNPs-Cy5.5-A&C consisted of two particles, AuNPs-Cy5.5-AK and AuNPs-Cy5.5-CABT, which were respectively modified with Ala–Ala–Asn–Cys–Lys (AK) and 2-cyano-6-aminobenzothiazole (CABT). We showed that AuNPs-Cy5.5-A&C could be selectively activated by AEP to aggregate and emit strong fluorescence. Moreover, AuNPs-Cy5.5-A&C displayed a general applicability in various cell lines and its florescence intensity correlated well with AEP activity in these cells. In the brain of APP/PS1 transgenic mice , AEP activity was increased at an early disease stage of AD that precedes formation of senile plaques and cognitive impairment. Pharmacological inhibition of AEP with δ-secretase inhibitor 11 (10 mg kg−1, p.o.) reduced production of β-amyloid (Aβ) and ameliorated memory loss. Therefore, elevation of AEP is an early sign of AD onset. Finally, we showed that live animal imaging with this AEP-responsive probe could monitor the up-regulated AEP in the brain of APP/PS1 mice.Conclusion sThe current work provided a proof of concept that assessment of brain AEP activity by in vivo imaging assay is a potential biomarker for early diagnosis of AD.Graphical abstract
研究领域:
神经科学
·
生物医学工程
·
分子生物学
·
神经退行性疾病
✓ 已验证
DOI
PubMed
高影响因子
ENERGY & FUELS
·
2025
·
IF 5.3
Yan Huang,Tao Song,Zhiyuan Dong,Lehang Liang,Weixin Kong,Xinling Zhong,Tao Wang,Mengxiang Fang,Wei Li,Sujing Li
In order to address the limitations of traditional organic amine absorbent, such as the limited CO2 absorption loading, difficulties in CO2 product regeneration, and high regeneration energy consumption during regeneration, a mixed amine absorbent consisting of 7.5 wt % 1,5-diamino-2-methylpentane (DA2MP) + 7.5 wt % N-aminoethylpiperazin (AEP) + 15 wt % N-methyldiethanolamine (MDEA) (D-A-M) has been developed. This formulation employed linear amine DA2MP as the primary absorbent, while cyclic amine AEP and tertiary amine MDEA served as the auxiliary absorbents. The development was achieved through a rigorous screening process. The D-A-M absorbent demonstrated a CO2 absorption capacity of 0.78 mol CO2·mol–1 and a regeneration efficiency of 80.38%. Additionally, it exhibited excellent cycling stabilities over five absorption–desorption cycles with the regeneration efficiency consistently exceeding 70%. The reaction mechanism between the absorbent and CO2, as revealed by 13C NMR analysis, involves the sequential reaction of primary, secondary, and tertiary amine groups. Electrochemical corrosion tests indicated that the CO2-saturated D-A-M absorbent showed significantly reduced corrosivity compared to the saturated 30 wt % MEA absorbent. In the pilot-scale experiment, the D-A-M absorbent achieved a CO2 capture rate of 97.5% and corrected regeneration energy consumption of 3.27 GJ·ton CO2–1, highlighting its considerable potential for industrial application.
✓ 已验证
产品关联
DOI
高影响因子
Greenhouse Gases-Science and Technology
·
2025
·
IF 2.8
Xiaoming Xu,Ying Xie,Yang Xia,Mengxiang Fang,Jie Cheng,Chenxiang Yao,Zihao Liu,Hao Xiang,Nengchuang Zhu,Gengsheng Liu
With the increasing global energy demand and its environmental impacts, carbon capture, utilization, and storage technology (CCUS) has become a crucial means to reduce CO 2 emissions and address climate change. This study investigated the thermal and oxidative degradation characteristics of two amine blend absorbents, methyldiethanolamine (MDEA)/piperazine (PZ) and MDEA/2-(piperazin-1-yl) ethanamine (AEP) and explored the effect of sulfur compounds on their stability. The experimental results indicated that MDEA/PZ exhibited a lower thermal degradation rate, whereas MDEA/AEP, due to the thermal instability of the AEP component, showed poorer degradation performance. In terms of oxidative degradation, monoethanolamine (MEA) demonstrated a higher oxidative degradation rate, whereas MDEA/PZ and MDEA/AEP exhibited lower oxidative degradation rates. The addition of inhibitors reduced the oxidative degradation rates of MDEA/PZ and MDEA/AEP, with antimony potassium tartrate (APT) proving to be the most effective inhibitor. In sulfur-containing environments, sulfur compounds (hydrogen sulfide and carbonyl sulfide) significantly enhanced both the thermal and oxidative degradation rate of MDEA/PZ and MDEA/AEP, particularly for MDEA/AEP, where the thermal degradation rate increased by 25.2%. This study revealed the impact of sulfur compounds on the thermal and oxidative degradation of absorbents. The findings provide important experimental data and theoretical support for optimizing the stability of carbon capture absorbents.
✓ 已验证
产品关联
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2021
·
IF 7.312
Li Shen,Fan Liu,Yao Shen,Cheng Sun,Yuchi Zhang,Qiaoli Wang,Sujing Li,Wei Li
Biphasic solvents have been attracting increasing attention in CO 2 capture recently due to its energy-saving potential. However, most current biphasic solvents still face the problem of limited desorption performance. To solve the problem, a novel biphasic solvent of 1-(2-aminoethyl)piperazine (AEP)/1-propanol/H 2 O solution was proposed. The absorption capacity was 1.26 mol CO 2 ·mol −1 amine, which was 2.3 times that of MEA. The regeneration efficiency was 74% and the cyclic capacity after 5 absorption-desorption experiments was 0.90 mol CO 2 ·mol −1 amine, which was 2.7 times that of MEA. The absorption-desorption performance of AEP/1-propanol/H 2 O solution was much better compared with most current biphasic solvents. Meanwhile, AEP/1-propanol/H 2 O solution had a low regeneration heat of 2.74 GJ·t −1 -CO 2 , which was 27.9% lower than that of MEA. Moreover, 13 C NMR analysis was used to investigate the absorption-desorption mechanism and species distribution. The multiple amino groups in AEP could react with CO 2 to produce carbamate and carbonate. The generated absorption products were mainly concentrated in the CO 2 -rich phase, while the CO 2 -lean phase contained much 1-propanol. Most of the absorption products were decomposed during regeneration, so the high desorption performance was achieved. Overall, AEP/1-propanol/H 2 O solution is a promising energy-saving candidate for industrial CO 2 capture due to its competitive absorption-desorption performance and low regeneration energy.
研究领域:
化学工程
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
Scientific Reports
·
2024
·Vol. 14
·期 1
·1-16
·
IF 3.8
Wang Qian,Gong Dehong,Zhang Zhongxiao,Zhu Jiangdong,Luo Qingling
The high energy consumption of Carbon Capture Utilization and Storage(CCUS) promotes the research of integrated absorption and mineralization technology. A novel DEEA/AEP biphasic absorbent is used to absorb \(\hbox {CO}_{2}\) . After phase separation, only one phase enriched with the absorption product is sent into the mineralization system, then reacts with \(\hbox {Ca(OH)}_{2}\) to produce \(\hbox {CaCO}_{3}\) at normal temperature and pressure. The effects of temperature, Ca/C and dispersion of the system were investigated, and cyclic utilization experiment was also conducted. The process was characterized and analyzed by Thermal gravimetric analysis (TGA), X-ray diffraction (XRD), and nuclear magnetic resonance ( 13 C NMR). The results showed that at 50 \(^{\circ }\) C, Ca/C = 1, the extent of mineralization of \(\hbox {CO}_{2}\) -enriched phase mineralization was 95.73% after ultrasonic treatment for 30 min. During 6 cycles, the volume percentage of \(\hbox {CO}_{2}\) -enriched phase could be maintained at 52–55%, and the \(\hbox {CO}_{2}\) load was 11.92mol/L. The results above indicate that the \(\hbox {CO}_{2}\) -enriched phase mineralization technology after absorption by biphasic absorbent can effectively capture \(\hbox {CO}_{2}\) , realize recycling, and reduce CCUS energy consumption.
研究领域:
环境科学
·
化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 12.5
Zehui Shang,Chunying Zhu,Taotao Fu,Youguang Ma
A liquid-liquid AEP/DEEA/n-butanol/H 2 O absorbent is developed. • Multi-variable optimization of absorption and desorption is performed. • NMR reveals carbamate and bicarbonate/carbonate absorption pathways. • Desorption energy AEP/DEEA/n-butanol/H 2 O is 66% of that of 30 wt% MEA/H 2 O. High regeneration energy remains a major obstacle to the practical application of alkanolamine-based absorbents for CO 2 capture. To reduce the regeneration energy demand, a liquid-liquid phase-change absorbent composed of polyamine 1-(2-aminoethyl)piperazine (AEP), 2-(diethylamino)ethanol (DEEA), n-butanol and H 2 O was developed. The effects of composition and operating conditions on the absorption–desorption performance were systematically investigated, identifying 1.8:1.2 AEP/DEEA, 4:3 n-butanol/H 2 O, 30 wt% total amine, and 303.15 K as suitable microchannel absorption conditions. 13 C and 1 H NMR analyses showed that AEP mainly absorbed CO 2 via the carbamate pathway, whereas the tertiary amine site of AEP and DEEA mainly promoted CO 2 hydration to form HCO 3 − /CO 3 2− . Under the optimal composition of 18 wt% AEP, 12 wt% DEEA, 40 wt% n-butanol, and 30 wt% H 2 O, the volume fraction and CO 2 loading of the rich phase reached 48.68% and 3.42 mol CO 2 ·kg −1 solution, respectively. Under desorption at 363.15 K with 2 mL lean-phase addition for 60 min, the desorption efficiency and cyclic capacity reached 58.91% and 1.95 mol CO 2 ·kg −1 solution, respectively, while the apparent desorption energy consumption was only 66% of that of 30 wt% MEA. Overall, this phase-change absorbent achieved effective synergy among rapid absorption, reversible regeneration, and phase-separation intensification, demonstrating its potential for low-energy CO 2 capture.
✓ 已验证
产品关联
DOI
高影响因子
RSC Chemical Biology
·
2022
·Vol. 3
·期 10
·1276-1281
·
IF 0
Xuan Ding,Ziyi Wang,Bin Zheng,Shengchao Shi,Yibing Deng,Hanyang Yu,Peng Zheng
Enzymatic protein ligation has become the most powerful and widely used method for high-precision atomic force microscopy single-molecule force spectroscopy (AFM-SMFS) study of protein mechanics. However, this methodology typically requires the functionalization of the glass surface with a corresponding peptide sequence/tag for enzymatic recognition and multiple steps are needed. Thus, it is time-consuming and a high level of experience is needed for reliable results. To solve this problem, we simplified the procedure using two strategies both based on asparaginyl endopeptidase (AEP). First, we designed a heterobifunctional peptide-based crosslinker, GL-peptide-propargylglycine, which links to an N3-functionalized surface via the click reaction. Then, the target protein with a C-terminal NGL sequence can be immobilized via the AEP-mediated ligation. Furthermore, we took advantage of the direct ligation between primary amino in a small molecule and protein with C-terminal NGL by AEP. Thus, the target protein can be immobilized on an amino-functionalized surface via AEP in one step. Both approaches were successfully applied to the AFM-SMFS study of eGFP, showing consistent single-molecule results.
研究领域:
生物化学
·
生物物理学
·
分子生物学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
危险品化学品经营许可证（带存储）