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AFB1抗体
Biomedical Optics Express · 2023 ·Vol. 14 ·期 9 ·4660-4676 · IF 3.4

Humanoid-shaped WaveFlex biosensor for the detection of food contamination

Ragini Singh,Wen Zhang,Xuecheng Liu,Bingyuan Zhang,Bingyuan Zhang,Santosh Kumar

High-toxicity secondary metabolites called aflatoxin are naturally produced by the fungus Aspergillus. In a warm, humid climate, Aspergillus growth can be considerably accelerated. The most dangerous chemical among all aflatoxins is aflatoxin B1 (AFB1), which has the potential to cause cancer and several other health risks. As a result, food forensicists now urgently need a method that is more precise, quick, and practical for aflatoxin testing. The current study focuses on the development of a highly sensitive, specific, label-free, and rapid detection method for AFB1 using a novel humanoid-shaped fiber optic WaveFlex biosensor (refers to a plasmon wave-based fiber biosensor). The fiber probe has been functionalized with nanomaterials (gold nanoparticles, graphene oxide and multiwalled carbon nanotubes) and anti-AFB1 antibodies to enhance the sensitivity and specificity of the developed sensor. The findings demonstrate that the developed sensor exhibits a remarkable low detection limit of 34.5 nM and exceptional specificity towards AFB1. Furthermore, the sensor demonstrated exceptional characteristics such as high stability, selectivity, reproducibility, and reusability. These essential factors highlight the significant potential of the proposed WaveFlex biosensor for the accurate detection of AFB1 in diverse agricultural and food samples.© 2023 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement

研究领域: 食品科学 · 生物技术 · 光学 · 纳米技术 · 毒理学
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OPTICS EXPRESS · 2023 ·Vol. 31 ·期 3 ·4783-4802 · IF 3.8

Plasmonic sensor based on offset-splicing and waist-expanded taper using multicore fiber for detection of Aflatoxins B1 in critical sectors

Xuecheng Liu,Ragini Singh,Muyang Li,Guoru Li,Rui Min,Carlos Marques,Bingyuan Zhang,Bingyuan Zhang,Santosh Kumar

In this work, authors have developed a portable, sensitive, and quick-response fiber optic sensor that is capable of detection of Aflatoxins B1 (AFB1) quantitatively and qualitatively. Using multi-mode fiber (MMF) and multi-core fiber (MCF), the MMF-MCF-MCF-MMF fiber structure based on symmetric transverse offset splicing and waist-expanded taper is fabricated. The evanescent waves are enhanced to form a strong evanescent field by etching the fiber surface with hydrofluoric acid. To successfully excite the localized surface plasmon resonance phenomenon, gold nanoparticles are deposited on the optical fiber probe's surface. Further, to modify the fiber optic probes, Niobium carbide (Nb2CTx) MXene and AFB1 antibodies are functionalized. Nb2CTx MXene is employed to strengthen the biocompatibility of the sensor and increase the specific surface area of the fiber probe, while AFB1 antibody is used to identify AFB1 micro-biomolecules in a specific manner. The reproducibility, reusability, stability, and selectivity of the proposed fiber probe are tested and validated using various concentration of AFB1 solutions. Finally, the linear range, sensitivity, and limit of detection of the sensing probe are determined as 0 - 1000 nM, 11.7 nm/µM, and 26.41 nM, respectively. The sensor offers an indispensable technique, low-cost solution and portability for AFB1-specific detection in agricultural products and their byproducts with its novel optical fiber structure and superior detecting capability. It is also useful for marine species like fish and consequently affecting health of human body.

研究领域: 光学传感 · 食品安全 · 生物医学工程 · 材料科学 · 环境监测
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