TALANTA
·
2023
·
IF 6.1
Yiqian Kong,Zongyi Li,Qi Liu,Juncheng Song,Yinghua Zhu,Jinping Lin,Lili Song,Xiangyang Li
In this work, V 2 C Mxene nano-enzyme materials (V 2 C MNs) with excellent peroxidase-like activity and fluorescence quenching performance were prepared, and it was modified using 6-carboxyfluorescein-labelled aptamers (ssDNA-FAM) to construct a novel dual-mode sensor V 2 C@ssDNA-FAM, with detection limits of 0.0477 ng mL −1 and 0.2789 ng mL −1 of fluorescence (linear range of 0.1–550 ng mL −1 ) and colorimetric (linear range of 1–1000 ng mL −1 ) modes, respectively. Meanwhile, an ANN intelligent detection platform has been constructed, which could automatically track and analyze the fluorescence and colorimetric signal of the detection system through machine learning and immediately obtain the AFB 1 concentration, and the detection limits of the fluorescence (linear range of 0.1–500 ng mL −1 ) and colorimetric (linear range of 1–800 ng mL −1 ) channels of it were 0.0905 ng mL −1 and 0.6845 ng mL −1 , respectively. The recovery rates of fluorescence, colorimetric sensing detection and ANN-assisted fluorescence and colorimetric sensing detection of real samples ranged from 95.40% to 101.76%. The method constructed in this work was superior to most existing literature reports and had great potential for application in the field of food quality testing.
研究领域:
食品安全检测
·
生物传感
·
人工智能辅助分析
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nano Materials Science
·
2025
·
IF 17.9
Yaqing Zhou,Zunquan Zhao,Yang Cao,Yaqing Zhao,Aimei Zhang,Ailing Zhang,Jialei Bai,Yuan Peng
Covalent organic framework (COF) materials have the advantages of good chemical stability, regular pores, high specific surface area, and it is widely used in adsorption, catalysis, and biosensors. However, the construction of photonic crystal (PC) with COF remains a challenge. In this work, we developed a new type of COF PC, which had an inverse opal array structure, called covalent organic framework - inverse opal photonic crystal (COF-IOPC). The COF-IOPC was prepared by sacrificial template method, which was simple and rapid. The SiO 2 microspheres was used to prepare the opal template through the restricted meniscus self-assembly method, and then, the COF pre-reaction solution was added to fulfill the crevices of the opal template. By controlling the size of the SiO 2 particles of the opal template, the photonic band gap of the COF-IOPC could be manipulated to exhibit different structural colors. Furthermore, the COF-IOPC could be used to detect Aflatoxin B 1 (AFB 1 ) without label. The spiked real samples were detected through the national standard method Liquid Chromatography - Tandem Mass Spectrometry (LC-MS/MS) as comparison, and the results indicated that COF-IOPC sensing method had a well accuracy with the LC-MS/MS method. The successful construction of this novel material further expands the application field of COF material.
研究领域:
材料科学
·
分析化学
·
生物传感器
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
Chemosensors
·
2025
·Vol. 13
·期 9
·342
·
IF 3.7
Juncheng Chen,Caizhang Wu,Zhike Zhao,Ruihao Xue
An electrochemical aptamer sensor for the sensitive detection of aflatoxin B1(AFB1) in corn samples was developed using nanocomposites loaded with copper nanoparticles (CuNPs) on zeolitic imidazolate framework-8 (ZIF-8), which were modified on a glassy carbon electrode (GCE). The CuNPs@ZIF-8 nanocomposites served as modifying materials for electrodes, exhibiting a high specific surface area and excellent compatibility with aptamers, thereby enhancing the electron transfer rate and increasing the aptamer loading and immobilization efficiency. The electrochemical properties before and after modification were investigated and validated using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) techniques, while the sensor’s performance was analyzed through quantitative detection via differential pulse voltammetry (DPV). Furthermore, various conditions, including the volume of ZIF-8 dispersion, electrodeposition time of copper nanoparticles, aptamer concentration, and AFB1incubation time, were optimized. The results indicated that the sensor exhibited a wide linear range (10.0 to 1.0 × 106pg/mL), with a lower limit of detection (LOD) of 1.13 pg/mL under optimized conditions, outperforming other reported aptamer sensors. The spiked recoveries in corn samples ranged from 96.663% to 105.72%. In conclusion, this sensor presents a novel solution for low-cost and high-sensitivity detection of AFB1.
研究领域:
分析化学
·
食品安全
·
电化学传感器
·
纳米材料
·
环境监测
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Zhihui Wu,Da-Wen Sun,Hongbin Pu,Qingyi Wei,Xuanran Lin
Mycotoxin B1 (AFB1) contamination in agricultural products pose a deadly danger to animal and human health and its rapid and reliable detection is thus very important. Herein, a ratiometric surface-enhanced Raman scattering (SERS) aptasensor for AFB1 detection was developed, in which 1,2-bis(4-pyridyl) ethylene (BPE) was used to trigger the assembly of Au nanoparticle dimers (AuNP dimers) and form intensive SERS “hot spots”, and MXenes nanosheets could load aptamer-modified AuNP dimers due to the hydrogen bonding and the chelation between the phosphate groups of aptamers and the Ti ion of MXenes. With the presence of AFB1 preferentially binding to AFB1 aptamer, AuNP dimers were separated from MXenes nanosheets, leading to a decrease in SERS intensity. Regression analysis in the range from 0.001 to 100 ng·mL −1 showed the limit of detection (LOD) being 0.6 pg·mL −1 in standard solution, indicating that the great prospects of the AuNP dimers/MXenes SERS substrate for detecting AFB1.
研究领域:
分析化学
·
纳米技术
·
食品安全
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）