JOURNAL OF FOOD PROCESS ENGINEERING
·
2024
·Vol. 47
·期 6
·e14654
·
IF 2.7
Chengquan Wang,Chengdong Gu,yanna Rong,Xin Zhao,Lu Qian,Mengting Liu,Xingyi Huang,Jing Qian
In this work, a paper-based electrochemical aptamer sensor was developed for the detection of aflatoxin B1 (AFB1) using a combination of MXene–Ti 3 C 2 T x and nucleic acid aptamers. The prepared single-layer or few-layer MXene suspension is suction-filtered onto MXene paper, which is cut to prepare MXene electrodes. To accomplish AFB1 specific detection, an amino-labeled AFB1 aptamer is mounted on the surface of the carboxy-functionalized MXene electrode. When AFB1 is present, it particularly binds to the aptamer to form a 3D structure, reducing the efficiency of electron transmission on the sensor surface. The difference in impedance signal change at the electrode/electrolyte interface is used to quantify AFB1. The results indicated that the detection range is 0.05–100 ng/mL, the detection limit is 0.04 ng/mL, and the recovery rate of AFB1 in corn samples is 97.8%–111.52% with the optimal detection conditions. The MXene paper-based label-free aptasensor is versatile and can detect different targets by simply swapping out the aptamers of different targets. The sensor also has a wide range of applications in food analysis and environmental testing. Practical applications A paper-based electrochemical aptamer sensor was developed to detect aflatoxin B1 using a combination of MXene–Ti 3 C 2 T x and nucleic acid aptamers.The design is based on the preparation of MXene electrodes by pumping and filtering monolayer or multilayer MXene suspensions onto MXene paper and cutting.The MXene paper-based label-free aptamer sensor was designed to be versatile, allowing the detection of different targets by simply replacing the aptamer with one from a different targets.
研究领域:
食品安全
·
环境科学
·
生物传感技术
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Wenting Li,Yongqiang Shi,Xinai Zhang,Xuetao Hu,Xiaowei Huang,Nini Liang,Tingting Shen,Xiaobo Zou,Jiyong Shi
Herein, a bifunctional electrochemical biosensor based on the DNA tetrahedral scaffolds (TDNs) was proposed, OTA@TDNs and AFB1@TDNs were adopted for electrochemical signal output in response to OTA and AFB1 concentration, simultaneously. In order to increase the conductivity of the biosensor, highly porous gold (HPG) was loaded on electrode surface by pulse electrodeposition. Under optimal conditions, the P Fc displayed a linear range with AFB1 concentration between 0.05 ∼ 360 ng·mL −1 with the LOD of 3.5 pg·mL −1 . And the P MB selective and sensitive responses to OTA are achieved with a linear range of 0.05 ∼ 420 ng·mL −1 and a LOD of 2.4 pg·mL −1 . This biosensor has high sensitivity, selectivity and stability for OTA and AFB1 detection in peanut samples. The approach streamlines the experimental procedure, leading to significantly improve the detection efficiency of mycotoxins. Collectively, this method suggest a novel approach for the detection and monitoring of OTA and AFB1 in food sample.
研究领域:
生物技术
·
食品安全检测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CONTROL
·
2023
·
IF 6
Jinxiang Wei,Yifei He,Zhiyi Song,Imran Mahmood Khan,Zhouping Wang,Caiyun Jiang,Xiaoyuan Ma
Herein, a surface-enhanced Raman scattering (SERS) and fluorescence dual-signal aptasensor has been developed for aflatoxin B 1 (AFB 1 ) detection. Fluorescence signal source CdTe quantum dots were decorated on the outer of silica nanoparticles and further functionalized with AFB 1 aptamer. Raman reporter molecules DTNB were decorated on the surface of gold nanoparticles (AuNPs) together with the aptamer complementary chains. The two parts of nanomaterials were combined by the base complementary pairing principle, to form satellite nanostructures with high SERS and low fluorescence signals following LSPR and FRET effect. The addition of AFB 1 led to the shedding of AuNPs due to the higher sensitivity of the aptamer towards the target and resulting in recovered fluorescence and decreased SERS signals. The developed method showed a linear relationship in the range of 10 −4 -10 3 ng/mL, with detection limit of 0.094 pg/mL. This technology had strong application prospects due to simplicity of operation and high accuracy.
研究领域:
分析化学
·
食品安全
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Lanqing Yang,Qiuya Li,Baoqing Bai,Tao Bo,Ligang Yu,Xu Jing,Shu Qin,Huilin Liu,Ying Zhang,Yukun Yang
Aflatoxin B1 (AFB1), a highly toxic mycotoxin prevalent in agricultural products, poses severe health threats. Addressing the critical need to mitigate AFB1-induced food safety risks, the primary objective of this study was to develop an accurate and ultrasensitive colorimetric/electrochemical (CL/EC) dual-mode aptasensor for AFB1 detection. To achieve this aim, we employed 3,3′,5,5′-tetramethylbenzidine (TMB) as a dual-function indicator. The catalytic oxidation of TMB generated oxidized oxTMB, concurrently producing colorimetric and electrochemical signal. Central to realizing the sensor's dual functionality, BSA@HOF-101 nano-enzymes were generated by in situ encapsulation of bovine serum albumin (BSA) in hydrogen-bonded organic frameworks (HOFs), which were indispensable enzyme mediators for the dual function of TMB. To specifically detect AFB1, BSA@HOF-101 was coupled with aptamer (Apt). The CL/EC dual-mode aptamer sensor offered detection of AFB1 across a concentration range from 10 −11 to 10 −7 g/mL. In addition, the sensor was successfully applied to the detection of AFB1 in corn and peanut samples with recoveries as high as 97.4 %–104.0 %. Collectively, this work established a robust analytical strategy with a dual-mode readout, providing a promising solution for the rapid and precise on-site monitoring of AFB1 contamination.
研究领域:
分析化学
·
纳米技术
·
食品安全
·
生物传感器
·
环境监测
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2020
·Vol. 167
·期 2
·027508
·
IF 3.721
Kaili Cui,Guanghan Li,Luyan Wang,Wenjuan Guo,Meishan Pei
In this work, a novel ON/OFF aptasensor was designed with a composite film consisting of carboxylated graphene oxide (GO) and poly(4-vinyl pyridine) (P4VP) for the accurate and sensitive detection of AFB1. The "on" and "off" behavior of the electrochemical sensor could be controlled by the pH sensitive P4VP. Through continuous switching of solution pH value below and above pKa of P4VP (pKa = 4.9), the conversion in the reversible electron transfer between protonized or deprotonized P4VP and the electrochemical probe was used to control the "ON" and "OFF" state of the electrochemical sensor. The condition was optimized when the electrochemical sensor is kept "ON" state, finding that the aptasensor showed highest current signal and the best electrochemical performance at pH = 4. Here the addition of P4VP not only could significantly improve the current signal at pH = 4 but also increase the stability of the modified electrode. Carboxylated GO was able to form an amide linkage with an amino-modified aptamer to fix the aptamer more firmly and increase the immobilized quantity of aptamer. On the basis of the excellent electrochemical performance of GO-P4VP composite, the designed biosensor exhibited a wide linear range from 0.01 to 50 ng ml−1 and a low detection limit of 14.75 pg ml−1.
研究领域:
分析化学
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生物传感
·
食品安全检测
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FOOD AND DRUG ANALYSIS
·
2022
·
IF 6.157
Wenwen Chen,Qiang Chen,Wei Zhang,De Zhang,Zhi Yu,Ying Song,Xiubing Zhang,Dejiang Ni,Pei Liang
The problem of aflatoxin contamination emerged gradually in the field of food safety. Surface-enhanced Raman spectroscopy (SERS) is an ultra-sensitive and non-destructive spectroscopy technology with extensive application prospects in the detection field. In this paper, with the detection of AFB1 as the target, Au@Ag NPs substrate with uniform morphology and strong SERS effect was prepared. Furthermore, the intermediates formed by hydrogen bonding between AFB1 and melamine facilitate the binding of toxin molecules to the substrate. Moreover, the AFB1-melamine-Au@Ag NPs detection system was established by optimizing the melamine dosage, and the limit of detection can reach 10 −8 mol/L (M). In this method, AFB1 (concentration range of 10 −4 M – 10 −7 M) in tea oil, the Raman signal intensity of AFB1 shows an excellent linear, logarithmic relationship, and the correlation coefficient is 0.9685. Therefore, this work has achieved simple, sensitive, and stable AFB1 detection and has broad application potential in food safety detection.
研究领域:
分析化学
·
食品科学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Xueming He,Yue Zhang,Xiaoyun Yang,Min Chen,Yanyan Pang,Fei Shen,Yong Fang,Qin Liu,Qiuhui Hu
An optical detection platform based on laser induced spectroscopy and double integrating spheres techniques was developed to obtain absorption ( μ a ), reduced scattering coefficients ( μ' s ) and fluorescence intensity of oil. The validation experiment carried on liquid phantoms showed that the developed system could achieve high linearity, and the results of spectra analysis indicated that the fluorescence intensity has a significant negative correlation with both μ a and μ' s . A total of 1620 oils with six categories were detected. The reason for the difference of fluorescence and μ a spectra was analyzed by comparing the measured chlorophyll, polyphenol and α-tocopherol contents. Linear discriminant analysis combined with principal component analysis based on fluorescence and μ a spectra was employed, to calibrate the AFB 1 classification models. The discrimination results manifested that by integrating μ a with fluorescence signal, the correct classification rate could be improved by more than 10%, and the false negative rate was greatly reduced.
研究领域:
食品科学
·
光学检测
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD AND CHEMICAL TOXICOLOGY
·
2024
·
IF 3.9
Hong Huang,Ziyan Li,Zhanghua Qi,Linxi Ma,Gang Hu,Changwei Zou,Tingtao Chen
The highly toxic aflatoxin B1 (AFB1) is considered one of the primary risk factors for hepatocellular carcinoma, while effective measures after AFB1 exposure remain to be optimized. This study utilized cell-surface-display technique to construct an engineered S. cerevisiae -pYD1-ScFv-AFB1 (S.C-AF) that specifically binds AFB1, and verified the potential mechanism of S.C-AF in vivo through AFB1-induced (gastric perfused with 0.3 mg/kg/d AFB1 per day) liver injury mouse model. In this experiment, the C57BL/6 mouse model of AFB1-induced liver injury was treated with S.C (gastric perfused with 1 × 10 9 CFU/mL S.C per day) and S.C-AF (gastric perfused with 1 × 10 9 CFU/mL S.C-AF per day) for 4 weeks, respectively. With probiotic properties optimized, S.C.-AF achieved an in vitro AFB1 binding capacity 1.7 times higher than S. cerevisiae. Furthermore, S.C-AF could alleviate AFB1-induced liver injury by reducing proinflammatory cytokine secretion and apoptotic protein expression, enhancing antioxidative capacity via Nrf2 activation, and simultaneously reversing intestinal tight junction protein deficiency, increasing intestinal barrier permeability, and improving intestinal dysbiosis caused by AFB1 exposure. S.C-AF alleviates AFB1-induced liver lesions, which might be a novel intervention to mitigate aflatoxin toxicity.
研究领域:
微生物学
·
毒理学
·
分子生物学
·
胃肠病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Wenpeng Jiang,Wenli Zhu,Qingli Yang,Wei Wu,Xiudan Hou
An electrochemical/colorimetric dual-model sensor was developed for the specific detection of aflatoxin B1, utilizing the high catalytic activity and magnetic separation properties of CoFe 2 O 4 @Au along with the conformational alterations in the hairpin DNA structure. The CoFe 2 O 4 @Au nanocomposite demonstrated excellent peroxidase-like activity, characterized by the relatively low Michaelis constant ( K m = 0.14 mM and 0.94 mM) and the relatively high reaction rate ( V max = 4.43 × 10 −8 M·s −1 and 9.01 × 10 −8 M·s −1 ). Besides, the visualization results could be obtained using a smartphone in colorimetric mode. The detection linear ranges of the electrochemical workstation, ultraviolet-visible spectrophotometer, and smartphone were 0.001–100 ng mL −1 , 0.05–100 ng mL −1 , and 0.1–100 ng mL −1 , respectively, with corresponding limits of detection were 0.34 pg mL −1 , 0.016 ng mL −1 , and 0.043 ng mL −1 . Furthermore, the matrix effect values of these methods were 91.03 %–112.96 % in peanut, corn, and wheat. This work provided a new direction to explore dual-mode sensor to improve the detection accuracy in real samples.
研究领域:
食品科学
·
分析化学
·
生物传感技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2023
·
IF 6.1
Yiqian Kong,Zongyi Li,Qi Liu,Juncheng Song,Yinghua Zhu,Jinping Lin,Lili Song,Xiangyang Li
In this work, V 2 C Mxene nano-enzyme materials (V 2 C MNs) with excellent peroxidase-like activity and fluorescence quenching performance were prepared, and it was modified using 6-carboxyfluorescein-labelled aptamers (ssDNA-FAM) to construct a novel dual-mode sensor V 2 C@ssDNA-FAM, with detection limits of 0.0477 ng mL −1 and 0.2789 ng mL −1 of fluorescence (linear range of 0.1–550 ng mL −1 ) and colorimetric (linear range of 1–1000 ng mL −1 ) modes, respectively. Meanwhile, an ANN intelligent detection platform has been constructed, which could automatically track and analyze the fluorescence and colorimetric signal of the detection system through machine learning and immediately obtain the AFB 1 concentration, and the detection limits of the fluorescence (linear range of 0.1–500 ng mL −1 ) and colorimetric (linear range of 1–800 ng mL −1 ) channels of it were 0.0905 ng mL −1 and 0.6845 ng mL −1 , respectively. The recovery rates of fluorescence, colorimetric sensing detection and ANN-assisted fluorescence and colorimetric sensing detection of real samples ranged from 95.40% to 101.76%. The method constructed in this work was superior to most existing literature reports and had great potential for application in the field of food quality testing.
研究领域:
食品安全检测
·
生物传感
·
人工智能辅助分析
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 10
·1-13
·
IF 5.3
Guo Liang,Zhou Shijin,Xue Jinyan,Liu Zenghui,Xu Shuqing,He Zhangxu,Yang Huaixia
A sandwich electrochemical sensor was fabricated based on multi-walled carbon nanotubes/ordered mesoporous carbon/AuNP (MWCNTs/CMK-3/AuNP) nanocomposites and porous core–shell nanoparticles Au@PdNPs to achieve rapid and sensitive detection of AFB 1 in complex matrices. MWCNTs/CMK-3/AuNP nanocomposite, which was prepared by self-assembly method, served as a substrate material to increase the aptamer loading and improve the conductivity and electrocatalytic activity of the electrode for the first signal amplification. Then, Au@PdNPs, which were synthesized by one-pot aqueous phase method, were applied as nanocarriers loaded with plenty of capture probe antibody (Ab) and signal molecule toluidine blue (Tb) to form the Au@PdNPs-Ab-Tb bioconjugates for secondary signal amplification. The sensing system could still significantly improve the signal output intensity even in the presence of ultra-low concentration target compound due to the dual signal amplification of MWCNTs/CMK-3/AuNP nanocomposites and Au@PdNPs-Ab-Tb. The method exhibited high selectivity, low detection limit (9.13 fg/mL), and strong stability to differentiate AFB 1 from other mycotoxins. Furthermore, the sensor has been successfully applied to the quantitative determination of AFB 1 in corn, malt, and six herbs, which has potential applications in food safety, quality control, and environmental monitoring. Graphical Abstract
研究领域:
分析化学
·
食品安全
·
环境监测
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Zenghui Liu,Jinyan Xue,Luyao Chen,Lele Ma,Huaixia Yang,Yaping Zhang,Mingsan Miao
A new, simple and low-cost electrochemical analysis approach employing click polymerization as the signal amplification strategy was developed for the highly sensitive assay of aflatoxin B 1 (AFB 1 ). First, the aptamer served as the capture probe for AFB 1 self-assembled on the surface of the Au electrode via Au-S bonding. After complementary DNA (c-DNA) modified with azide groups was hybridized with the aptamer, a massive click polymer was introduced as a signal tag via click chemistry. Finally, the captured AFB 1 competed with the c-DNA to bind the aptamer and the c-DNA was shed from the electrode surface and the current intensity was reduced. The electrochemical aptamer sensor is easy to operate, selective and sensitive, and also exhibits excellent reliability in detecting AFB 1 in actual samples. A rapid, simple, efficient and sensitive platform for AFB 1 analysis is proposed in this work, and the strategy can be further extended to trace analysis of other analytes, safety detection, environmental monitoring and disease diagnosis after replacing the corresponding capture probes, with promising applications in the fields of food hygiene and safety.
研究领域:
分析化学
·
生物化学
·
食品安全
·
环境监测
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）